The stability and maintenance mechanisms of the microbial ecosystem
| Project/Area Number |
20K15544
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 39060:Conservation of biological resources-related
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Suzuki Kenshi 東京大学, 大学院農学生命科学研究科(農学部), 特任助教 (80870188)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2021: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2020: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 微生物生態系 / 代謝ネットワーク / 環境保全 / 環境保全再生 / 環境再生 / モデル微生物生態系 |
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| Outline of Research at the Start |
生物資源の保全は社会にとって必要不可欠である一方で、自然環境は刻々と減少しており、失われた自然を再生することが重要な課題となっている。本研究では生命に必須な物質循環の中核を担う微生物生態系の形成プロセス解明を目指す。その為に、複数種の分離菌株を用いたモデル微生物生態系を構築し、その動態を個々の微生物の代謝とその繋がり（代謝ネットワーク）として捉る。また、代謝ネットワークを化学反応式に落とし込むことでし、これまで概念として捉えられてきた微生物生態系の安定性の熱力学的定量評価方法の構築を目指す。
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| Outline of Final Research Achievements |
The maintenance mechanisms of a microbial ecosystem and its stability were investigated in this study. Since the natural microbial community is too complex to analyze, five different phenol-degrading bacteria were incubated in phenol-fed chemostat culture as a model microbial community. Several equilibrium points of microbial communities were observed when the five strains were sequentially inoculated with several patterns. RNA-seq analysis suggested that phenol was occupied by the dominant strain and others utilized metabolites from the dominant strain when the community structure stabilized at an early incubation phase. On the other hand, it was more competitive for phenol in unstable culture. These results suggested that the emergence of metabolic network among microbial strains were significantly important in stabilizing a microbial community structure. Thus, the design of the metabolic network is essential to design and to control microbial community.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では微生物生態系の形成過程が最終的な微生物群集構造を変化させることを示した。また、微生物間で代謝ネットワークが形成できるか否かがシステムの安定性に重要であることを示唆しており、微生物生態系の形成、維持変遷に関わる根幹的知見を得ることができたと判断できる。また、複合微生物群のデザイン、制御において、微生物種だけでなく形成される代謝ネットワークを考慮する重要性を示し、微生物利用技術の発展そして地球環境保全および再生に寄与できることから社会的意義も高いと評価できる。
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] Imbalance in Carbon and Nitrogen Metabolism in <i>Comamonas testosteroni</i> R2 Is Caused by Negative Feedback and Rescued by L-arginine2021
Author(s)
Abd Rahman Jabir Mohd Din, Kenshi Suzuki, Masahiro Honjo, Koki Amano, Tomoka Nishimura, Ryota Moriuchi, Hideo Dohra, Hidehiro Ishizawa, Motohiko Kimura, Yosuke Tashiro, and Hiroyuki Futamata
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Journal Title
Microbes and Environments
Volume: 36
Issue: 4
Pages: n/a
DOI
NAID
ISSN
1342-6311, 1347-4405
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Fluctuation of Bacterial Interactions Enables Coexistence of Different Bacterial Strains in Chemostat Conditions2021
Author(s)
M. Honjo, K. Suzuki, K. Amano, Y. Saito, K. Takeda, M. Kimura, H. Ishizawa, Y. Tashiro, H. Futamata
Organizer
World Microbe Forum, American Society for Microbiology, Federation of European Microbiological Societies
Related Report
Int'l Joint Research
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