| Project/Area Number |
20K15723
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 43010:Molecular biology-related
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Yoshida Makoto 国立研究開発法人理化学研究所, 開拓研究本部, 協力研究員 (20791453)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2020: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | condensin / chromosome / mitosis / HEAT / phosphorylation / chromosomes / SMC / HEAT repeats / ATPase / condensation / assembly / コンデンシンII / 染色体軸 / クロマチン / 染色体 |
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| Outline of Research at the Start |
The condensin II multisubunit complex coordinates with condensin I to assemble mitotic chromosomes in vertebrates. Despite its fundamental importance, the molecular function of condensin II remains largely unknown. To understand the role of condensin II, Xenopus laevis egg extract assays have been established to analyze reconstituted mammalian condensin II complexes. Biochemical analyses of reconstituted mammalian condensin II and mutant complexes will determine the functions of individual subunits of condensin II in chromosome assembly.
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| Outline of Final Research Achievements |
Changes in chromosomal structure that occur during mitosis are essential for successful cell division. Condensin I and II are protein complexes that play important roles in compacting and maintaining chromosome structures. Although the function of condensin I subunits in the assembly of mitotic chromosomes is becoming clear, condensin II subunits remain poorly understood. To elucidate the function of condensin II and its five subunits, the complex was expressed and successfully purified. By testing various mutant complexes, the study was able to elucidate the functional roles of each subunit as well as the similarities and differences between condensin I and II.
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| Academic Significance and Societal Importance of the Research Achievements |
Mis-regulation of chromosome organization, primarily mediated by condensin protein complexes, can lead to the development of diseases and cancer. This study elucidates the mechanism of condensin II protein complex to understand how cell division is achieved without errors in chromosome organization.
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