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Functional analysis of RAB23 in formation of locomotory apparatus of plant spermatozoids

Research Project

Project/Area Number 20K15824
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 44030:Plant molecular biology and physiology-related
Research InstitutionNational Institute for Basic Biology

Principal Investigator

Minamino Naoki  基礎生物学研究所, 細胞動態研究部門, 特任助教 (20823256)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2020: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords膜交通 / RAB GTPase / 精子 / 鞭毛 / ゼニゴケ
Outline of Research at the Start

一部の陸上植物は,動物と同様に運動能をもつ雄性配偶子である精子を形成する.鞭毛および多層構造体は,植物精子の運動能発現のために必須の構造であるが,その構築システムについては未解明な点が多い.
RAB23は,膜交通の制御因子であるRAB GTPaseのひとつで,鞭毛および繊毛をもつ生物に保存されるという特徴的な分布を示し,鞭毛の形成や運動に重要な役割を果たすと考えられる.本研究では,タイ類ゼニゴケをモデルとしたRAB23の分子機能の解明を通して,植物精子の鞭毛および多層構造体の構築システムの一端を明らかにする.

Outline of Final Research Achievements

Some plant lineages including bryophytes generate motile sperm cells called spermatozoids which harbor a locomotory apparatus such as a flagellum and multilayered structure. RAB23 is one of subfamilies of RAB GTPase which is a machinery component of the membrane trafficking system. In this study, we attempted to elucidate the function of RAB23 in plant spermatozoids using the liverwort, Marchantia polymorpha. To establish an analytical foundation, we delineated the developmental stages and observed the dynamics of various organelles during spermiogenesis in M. polymorpha. We found that MpRAB23 acts on forming flagella and should be involved in proper flagellar formation during spermiogenesis.

Academic Significance and Societal Importance of the Research Achievements

植物の精子の運動装置形成の仕組みはこれまであまり明らかになっておらず、本研究はその一端を明らかにしました。また鞭毛は植物だけでなく動物にも保存された構造であり、本研究により明らかになったRAB23の鞭毛形成における役割は、他の生物における鞭毛形成の仕組みを考察するうえでも重要な知見になることが期待されます。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (8 results)

All 2023 2022 2020

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 5 results) Presentation (3 results)

  • [Journal Article] Autophagy regulates plastid reorganization during spermatogenesis in the liverwort Marchantia polymorpha2023

    • Author(s)
      Norizuki Takuya、Minamino Naoki、Sato Miyuki、Ueda Takashi
    • Journal Title

      Frontiers in Plant Science

      Volume: 14 Pages: 1-8

    • DOI

      10.3389/fpls.2023.1101983

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Remodeling of organelles and microtubules during spermiogenesis in the liverwort Marchantia polymorpha2022

    • Author(s)
      Minamino Naoki、Norizuki Takuya、Mano Shoji、Ebine Kazuo、Ueda Takashi
    • Journal Title

      Development

      Volume: 149 Issue: 15 Pages: 1-14

    • DOI

      10.1242/dev.200951

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Phylogenetic distribution and expression pattern analyses identified a divergent basal body assembly protein involved in land plant spermatogenesis2022

    • Author(s)
      Koshimizu Shizuka、Minamino Naoki、Nishiyama Tomoaki、Yoro Emiko、Sato Mayuko、Wakazaki Mayumi、Toyooka Kiminori、Ebine Kazuo、Sakakibara Keiko、Ueda Takashi、Yano Kentaro
    • Journal Title

      New Phytologist

      Volume: 236 Issue: 3 Pages: 1182-1196

    • DOI

      10.1111/nph.18385

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Dynamic rearrangement and autophagic degradation of mitochondria during spermiogenesis in the liverwort Marchantia polymorpha2022

    • Author(s)
      Norizuki Takuya、Minamino Naoki、Sato Miyuki、Tsukaya Hirokazu、Ueda Takashi
    • Journal Title

      Cell Reports

      Volume: 39 Issue: 11 Pages: 110975-110975

    • DOI

      10.1016/j.celrep.2022.110975

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Membrane trafficking functions of the ANTH/ENTH/VHS domain‐containing proteins in plants2022

    • Author(s)
      Feng Yihong、Hiwatashi Takuma、Minamino Naoki、Ebine Kazuo、Ueda Takashi
    • Journal Title

      FEBS Letters

      Volume: 596 Issue: 17 Pages: 2256-2268

    • DOI

      10.1002/1873-3468.14368

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 基部陸上植物タイ類ゼニゴケにおけるESCRT-III複合体の機能解析2022

    • Author(s)
      南野尚紀、法月拓也、真野昌二、海老根一生、上田貴志
    • Organizer
      第64回日本植物生理学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] イメージング・AI技術を駆使して植物精子の形成と運動の仕組みを理解する2022

    • Author(s)
      南野尚紀、法月拓也、藤井春樹、八野田奨、海老根一生、真野昌二、堀田一弘、上田貴志
    • Organizer
      日本植物学会第86回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] ゼニゴケ精子変態過程におけるオルガネラ動態の解析2020

    • Author(s)
      南野尚紀,海老根一生,上田貴志
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Research-status Report

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Published: 2020-04-28   Modified: 2024-01-30  

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