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post-translational modification of a transcription factor GTL1

Research Project

Project/Area Number 20K15827
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 44030:Plant molecular biology and physiology-related
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Shibata Michitaro  国立研究開発法人理化学研究所, 環境資源科学研究センター, 研究員 (20734817)

Project Period (FY) 2020-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2021: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2020: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords根毛 / シロイヌナズナ / 転写制御 / 栄養応答 / 転写因子 / 翻訳後修飾
Outline of Research at the Start

植物は生存戦略として、外部環境に応答した成長制御を行う。特に根の組織である「根毛」はその応答が顕著であり、土壌中の無機栄養素に応じてその長さが変わるという現象がよく知られている。一方、その現象の背景にある詳細な分子機構は明らかではない。これまでの研究から、転写因子GT2-LIKE1 (GTL1)が、根毛の成長を負に制御することが明らかとなった。そこで本研究計画では、植物が変動する環境に対し柔軟に適応するメカニズムへの理解を目指し、転写因子GTL1の活性を制御する分子機構の解明を行う。

Outline of Final Research Achievements

Root hair growth is tuned in response to the surrounding environment. While most previous studies focused on the enhancement of root hair growth during nutrient starvation, few studies investigated the root hair response in the presence of excess nutrients. I found that root hair growth of wild-type Arabidopsis plants is strongly suppressed with increasing nutrient availability. We further used gene expression profiling to analyze how excess nutrient availability affects root hair growth, and found that RHD6 subfamily genes, which are positive regulators of root hair growth, are down-regulated in this condition. On the other hand, defects in GTL1 and DF1, which are negative regulators of root hair growth, cause frail and swollen root hairs when excess nutrients are supplied. In conclusion, our data suggest that GTL1 and DF1 prevent unnecessary root hair formation under excess nutrient conditions.

Academic Significance and Societal Importance of the Research Achievements

これまで植物の栄養応答は「特定」の栄養素が「不足」した条件で研究が進められてきた。一方、本研究は「複数」の栄養素が「過剰」に存在する条件での応答に着目し、転写因子GTL1が栄養過剰条件において根毛の成長を適切に抑制するために必要な因子であることを明らかとした。昨今、過剰量の肥料が施されたことに由来する湖川の富栄養化や大気汚染などは大きな問題となっており、栄養過剰条件における植物の応答とその分子機構に切り込んだ本研究の成果は社会的にも学術的にも意義深いものである。

Report

(3 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • Research Products

    (8 results)

All 2022 2021 2020 Other

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (4 results) Remarks (2 results)

  • [Journal Article] Arabidopsis TBP-ASSOCIATED FACTOR 12 ortholog NOBIRO6 controls root elongation with unfolded protein response cofactor activity2022

    • Author(s)
      Kim June-Sik、Sakamoto Yuki、Takahashi Fuminori、Shibata Michitaro、Urano Kaoru、Matsunaga Sachihiro、Yamaguchi-Shinozaki Kazuko、Shinozaki Kazuo
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 119 Issue: 6

    • DOI

      10.1073/pnas.2120219119

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Trihelix transcription factors GTL1 and DF1 prevent aberrant root hair formation in an excess nutrient condition2022

    • Author(s)
      Michitaro Shibata, David S. Favero, Ryu Takebayashi, Arika Takebayashi, Ayako Kawamura, Bart Rymen, Yoichiroh Hosokawa, Keiko Sugimoto
    • Journal Title

      New Phytologist

      Volume: -

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Presentation] An environmental response on root hairs under phosphate starvation in Arabidopsis2022

    • Author(s)
      Michitaro Shibata, Ayako Kawamura, Keiko Sugimoto
    • Organizer
      第63回日本植物生理学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Excess nutrition suppresses Arabidopsis root hair growth2021

    • Author(s)
      Michitaro Shibata, Ayako Kawamura, Keiko Sugimoto
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Excess nutrition suppresses Arabidopsis root hair growth2021

    • Author(s)
      Michitaro Shibata, Ayako Kawamura, KeikoSugimoto
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Research-status Report
  • [Presentation] 栄養過剰条件下における シロイヌナズナ根毛の成長抑制機構2020

    • Author(s)
      柴田美智太郎、河村彩子、杉本慶子
    • Organizer
      日本植物学会 第84回大会
    • Related Report
      2020 Research-status Report
  • [Remarks] Researchmap

    • URL

      https://researchmap.jp/shibatamichi

    • Related Report
      2021 Annual Research Report
  • [Remarks] 理化学研究所 細胞機能研究チームのホームページ

    • URL

      http://cellfunction.riken.jp/

    • Related Report
      2020 Research-status Report

URL: 

Published: 2020-04-28   Modified: 2023-01-30  

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