Research for cancer invasion mechanism and responsible factor with intravital imaging technology

• Project/Area Number: 20K16192
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 49020:Human pathology-related
• Research Institution: Osaka University
• Principal Investigator: Matsui Takahiro 大阪大学, 大学院医学系研究科, 准教授 (50747037)
• Project Period (FY): 2020-04-01 – 2023-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 生体イメージング

Outline of Research at the Start

生体イメージング技術で担がんモデルマウスを観察し、腫瘍の発生初期から浸潤能獲得の過程における腫瘍細胞の“動き”を可視化する。そして腫瘍細胞の浸潤という現象から腫瘍の悪性化メカニズムを理解し、腫瘍の進行の早期検出が可能で、かつ臨床診断にも応用可能な因子を抽出する。

Outline of Final Research Achievements

Although it has long been recognized that the characteristics of tumor cell dynamics are extremely important factors in the progression of malignant tumors, it has been difficult to obtain information on cell dynamics from conventional tissue specimens. In this study, we observed model animals using intravital imaging technology and visualized the dynamics of tumor cells during tumor growth and invasion. As a result, we extracted the factors that can detect the tumor invasion at early stage and can be applied to the cancer diagnosis in clinical medicine. Moreover, we found that the established evaluation system can be actually applied to human tissue samples.

Academic Significance and Societal Importance of the Research Achievements

悪性腫瘍（がん）の早期発見については、現在の本邦の医療状況において最重要課題といえる課題であり、本研究で見出された成果の社会的意義は極めて大きい。また、組織病理学に立脚した基礎研究成果と光学イメージング技術とを融合した学際的な研究が、悪性腫瘍の理解に大きな意義があることを示すものでもある。現時点では国際的にも同様の研究の報告例はなく、世界的にも独自性の高い内容であり、学術的に大きな意義がある。

Research Products

• [Journal Article] PIK3CA mutation correlates with mTOR pathway expression but not clinical and pathological features in Fibfibroipose vascular anomaly (FAVA) (2022)
  • Author(s): Yumiko Hori, Katsutoshi Hirose, Michio Ozeki, Kenji Hata, Daisuke Motooka, Shinichiro Tahara, Takahiro Matsui, Masaharu Kohara, Hiroki Higashihara, Yusuke Ono, Kaishu Tanaka, Satoru Toyosawa & Eiichi Morii
  • Journal Title: Diagnostic Pathology Volume: 17 Issue: 1 Pages: 19-19
  • DOI: 10.1186/s13000-022-01199-3
  • Peer Reviewed / Open Access
• [Journal Article] Deep Learning Analysis of Histologic Images from Intestinal Specimen Reveals Adipocyte Shrinkage and Mast Cell Infiltration to Predict Postoperative Crohn Disease (2022)
  • Author(s): Kiyokawa H, Abe M, Matsui T, Kurashige M, Ohshima K, Tahara S, Nojima S, Ogino T, Sekido Y, Mizushima T, Morii E.
  • Journal Title: Am J Pathol . Volume: 22 Issue: 6 Pages: 106-7
  • DOI: 10.1016/j.ajpath.2022.03.006
  • Peer Reviewed / Open Access
• [Journal Article] A Novel Three-Dimensional Imaging System Based on Polysaccharide Staining for Accurate Histopathological Diagnosis of Inflammatory Bowel Diseases (2022)
  • Author(s): Nojima Satoshi、Ishida Shoichi、Terayama Kei、Matsumoto Katsuhiko、Matsui Takahiro、Tahara Shinichiro、Ohshima Kenji、Kiyokawa Hiroki、Kido Kansuke、Ukon Koto、Yoshida Shota Y.、Mitani Tomoki T.、Doki Yuichiro、Mizushima Tsunekazu、Okuno Yasushi、Susaki Etsuo A.、Ueda Hiroki R.、Morii Eiichi
  • Journal Title: Cellular and Molecular Gastroenterology and Hepatology Volume: 14 Issue: 4 Pages: 905-924
  • DOI: 10.1016/j.jcmgh.2022.07.001
  • Peer Reviewed / Open Access
• [Journal Article] Label‐free multiphoton excitation imaging as a promising diagnostic tool for breast cancer (2022)
  • Author(s): Matsui Takahiro、Iwasa Akio、Mimura Masafumi、Taniguchi Seiji、Sudo Takao、Uchida Yutaka、Kikuta Junichi、Morizono Hidetomo、Horii Rie、Motoyama Yuichi、Morii Eiichi、Ohno Shinji、Kiyota Yasujiro、Ishii Masaru
  • Journal Title: Cancer Science Volume: 113 Issue: 8 Pages: 2916-2925
  • DOI: 10.1111/cas.15428
  • Peer Reviewed / Open Access
• [Journal Article] Pancreatic fibrosis by extracellular volume fraction using Contrast-enhanced computed tomography and relationship with pancreatic cancer (2022)
  • Author(s): Hideyuki Fukui, Hiromitsu Onishi, Atsushi Nakamoto, Takahiro Tsuboyama, Takashi Ota, Keigo Yano, Yukihiro Enchi, Daisaku Yamada, Yu Takeda, Shogo Kobayashi, Yasunari Fukuda, Hidetoshi Eguchi, Takahiro Matsui, Mitsuaki Tatsumi, Noriyuki Tomiyama
  • Journal Title: European Journal of Radiology Volume: 156 Pages: 1-7
  • DOI: 10.1016/j.ejrad.2022.110522
  • Peer Reviewed
• [Journal Article] Tumor cell plasticity in endometrioid carcinoma is regulated by neuronal membrane glycoprotein M6?b (2022)
  • Author(s): Kusumoto Shinya、Ikeda Jun-Ichiro、Kurashige Masako、Maeno-Fujinami Etsuko、Tahara Shinichiro、Matsui Takahiro、Nojima Satoshi、Okuzaki Daisuke、Morii Eiichi
  • Journal Title: Oncology Letters Volume: 25 Issue: 2 Pages: 45-45
  • DOI: 10.3892/ol.2022.13631
  • Peer Reviewed
• [Journal Article] An immature inhibin‐α‐expressing subpopulation of ovarian clear cell carcinoma cells is related to an unfavorable prognosis (2021)
  • Author(s): Kusumoto Shinya、Kurashige Masako、Ohshima Kenji、Tahara Shinichiro、Matsui Takahiro、Nojima Satoshi、Hattori Satoshi、Morii Eiichi
  • Journal Title: Cancer Medicine Volume: 10 Issue: 5 Pages: 1485-1500
  • DOI: 10.1002/cam4.3801
  • Peer Reviewed / Open Access
• [Journal Article] Group 2 innate lymphoid cells support hematopoietic recovery under stress conditions (2021)
  • Author(s): Sudo Takao、Motomura Yasutaka、Okuzaki Daisuke、Hasegawa Tetsuo、Yokota Takafumi、Kikuta Junichi、Ao Tomoka、Mizuno Hiroki、Matsui Takahiro、Motooka Daisuke、Yoshizawa Ryosuke、Nagasawa Takashi、Kanakura Yuzuru、Moro Kazuyo、Ishii Masaru
  • Journal Title: Journal of Experimental Medicine Volume: 218 Issue: 5 Pages: 20200817-20200817
  • DOI: 10.1084/jem.20200817
  • Peer Reviewed / Open Access
• [Journal Article] A case of unusually persistent traumatic intrapulmonary hematoma (2021)
  • Author(s): Kawagishi Sachi、Kogita Yuya、Nakamichi Itsuko、Matsui Takahiro、Tokunaga Toshiteru
  • Journal Title: Respiratory Medicine Case Reports Volume: 34 Pages: 101507-101507
  • DOI: 10.1016/j.rmcr.2021.101507
  • Peer Reviewed
• [Journal Article] Nicotinamide N-methyltransferase is related to MELF pattern invasion in endometrioid carcinoma (2021)
  • Author(s): Tahara S, Nojima S, Ohshima K, Hori Y, Sato K, Kurashige M, Matsui T, Okuzaki D, Morii E.
  • Journal Title: Cancer Med . Volume: 10 Issue: 23 Pages: 8630-8640
  • DOI: 10.1002/cam4.4359
  • Peer Reviewed / Open Access
• [Journal Article] Deep Vein Thrombosis as an Initial Symptom of Malignant Tumor: A Case Report of Angiosarcoma in the Iliac Vein (2021)
  • Author(s): Matsui Takahiro、Nojima Satoshi、Morii Eiichi
  • Journal Title: Case Reports in Oncology Volume: 14 Issue: 3 Pages: 1542-1547
  • DOI: 10.1159/000519598
  • Peer Reviewed / Open Access
• [Journal Article] FAM111B enhances proliferation of KRAS ‐driven lung adenocarcinoma by degrading p16 (2020)
  • Author(s): Kawasaki Keisuke、Nojima Satoshi、Hijiki Sachiko、Tahara Shinichiro、Ohshima Kenji、Matsui Takahiro、Hori Yumiko、Kurashige Masako、Umeda Daisuke、Kiyokawa Hiroki、Kido Kansuke、Okuzaki Daisuke、Morii Eiichi
  • Journal Title: Cancer Science Volume: 111 Issue: 7 Pages: 2635-2646
  • DOI: 10.1111/cas.14483
  • Peer Reviewed / Open Access
• [Journal Article] Fibro-adipose vascular anomaly (FAVA): three case reports with an emphasis on the mammalian target of rapamycin (mTOR) pathway (2020)
  • Author(s): Hori Yumiko、Hirose Katsutoshi、Aramaki-Hattori Noriko、Suzuki Sachi、Nakayama Robert、Inoue Masanori、Matsui Takahiro、Kohara Masaharu、Toyosawa Satoru、Morii Eiichi
  • Journal Title: Diagnostic Pathology Volume: 15 Issue: 1 Pages: 98-98
  • DOI: 10.1186/s13000-020-01004-z
  • Peer Reviewed / Open Access
• [Journal Article] Nonlinear Optics with Near-Infrared Excitation Enable Real-Time Quantitative Diagnosis of Human Cervical Cancers (2020)
  • Author(s): Matsui Takahiro、Tamoto Ryo、Iwasa Akio、Mimura Masafumi、Taniguchi Seiji、Hasegawa Tetsuo、Sudo Takao、Mizuno Hiroki、Kikuta Junichi、Onoyama Ichiro、Okugawa Kaoru、Shiomi Mayu、Matsuzaki Shinya、Morii Eiichi、Kimura Tadashi、Kato Kiyoko、Kiyota Yasujiro、Ishii Masaru
  • Journal Title: Cancer Research Volume: 80 Issue: 17 Pages: 3745-3754
  • DOI: 10.1158/0008-5472.can-20-0348
  • Peer Reviewed / Open Access