| Project/Area Number |
20K16242
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 49050:Bacteriology-related
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| Research Institution | Osaka University |
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Principal Investigator |
Zwama Martijn 大阪大学, 産業科学研究所, 特任助教(常勤) (40827052)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2022: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | Multidrug resistance / Pathogens / Bacteria / Transporters / Phylogenetics / Evolution / Adaptation / Antibiotics / RND / Multidrug Resistance / MDR / Efflux pumps / AcrB / Transporter |
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| Outline of Research at the Start |
Multidrug resistance is a major threat to human health worldwide. With this research we aim to better understand drug resistance on a molecular level, by investigating RND efflux pumps from multiple previously unstudied bacteria. We will test substrate specificity patterns and inhibition efficiencies. We are also interested to see the possible coupling of the pumps with different outer membrane proteins: an area of recent interests for the development of efflux pump inhibitors.
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| Outline of Final Research Achievements |
Multidrug efflux pumps belonging to the RND superfamily from multiple Gram-negative bacteria have been cloned and expressed, and mutations have been introduced after meticulously studying the differences and similarities of hundreds of pumps. The results show interesting novel findings relevant and important to the field of multidrug resistance regarding the function of RND-type multidrug efflux pumps, their evolution, their conservation, and their function. During this research period, we have published research papers (as well as review articles) in multiple peer-reviewed journals.
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| Academic Significance and Societal Importance of the Research Achievements |
We expanded our understanding of multidrug resistance and provided novel insights into the function and adaptation of RND pumps. This has social significance as it contributes to healthcare strategies in combating multidrug resistance and addresses the global health challenge of drug resistance.
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