|Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|Outline of Research at the Start
Cholix toxin (ChxA) is a recently identified cytotoxin produced by Vibrio cholerae. The cytotoxic pathway and mouse lethality caused by liver injury has been gradually understood, however, the cell surface receptors are not yet known, which are necessary molecules for ChxA binding to target cells. My in silico analysis identified a potential toxin receptor. This study will evaluate my hypothesis by comparing toxin sensitivity of several cell lines expressing and non-expressing the candidate receptor molecule. In vitro binding assay and co-crystallization analysis will be also carried out.