Human induced pluripotent stem cell derived cardiomyocyte maturation by DNA integrative free-delivery of key regulators
| Project/Area Number |
20K17078
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 53020:Cardiology-related
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2021: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2020: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | Cardiac maturation / TNNI3 / hiPSC-CM / Stem Cell Research / Cell Cycle / cardiac maturation / tnni3 / small molecules / heart / epicardium / eht / tissue bioengineering / disease modeling / stem cells / cellular bioengineering / nanomedic / hiPSC-EPI / Heart |
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| Outline of Research at the Start |
1./ To conduct functional assays to assess cardiac functionality
2./ Elucidation of molecular mechanisms: (1) activation of gene repressors or activators or (2) activation of signaling cascades via kinase phosphorylations
3./ Developing a DNA integrative-free system for key factors delivery.
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| Outline of Final Research Achievements |
We extensively worked in developing safe methods to increase the maturation of hiPSC-derived cardiomyocytes to improve clinical applications in regenerative medicine.
Eventually, we developed methods to increase the adult-like properties of hiPSC-CM that can be transferred to clinical applications.
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| Academic Significance and Societal Importance of the Research Achievements |
We developed methods with clinical applications to increase the maturation of cardiac tissues derived from human iPS exploiting the control of the cardiac cell cycle.
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Report
(4 results)
Research Products
(8 results)
