| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2021: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2020: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Outline of Final Research Achievements |
The public database analysis revealed that the subunit of SWI/SNF complex such as SMARCA4, SMARCC1 co-localized with a certain cancer-associated transcription factor (TF) in promoter and enhancer regions of several cancer-related genes. Therefore, we performed co-immunoprecipitation experiments using antibodies against the TF and the subunit of SWI/SNF complex (SMARCA4, C1, C2, ARID1A). As a result, the precipitants by the antibody against the subunit of SWI/SNF complex (SMARCA4, C1, C2, ARID1A) contained this TF. On the other hand, the precipitants by the antibody raised against the TF somehow did not contain the subunit of SWI/SNF complex (SMARCA4, C1, C2, ARID1A). This might be because the TF makes complex with so many molecules. Therefore, although further investigation is required, our results suggest the possibility that this cancer-associated TF may be a new subunit of SWI/SNF complex.
|
