| Project/Area Number |
20K18103
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 56030:Urology-related
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| Research Institution | International University of Health and Welfare (2021-2022)
Nippon Medical School (2020) |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2021: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2020: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | spermatogenesis / testis specific lncRNAs / arsenic / long non-coding RNA / mouse testis / testis development / testis-specific lncRNAs / testis-specific lncRNA / mouse spermatogenesis / testis |
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| Outline of Research at the Start |
Long non-coding RNAs (lncRNAs) are key regulators in biological processes such as spermatogenesis. We newly identified mouse testis-specific lncRNAs: 1700108J01Rik and 1700101O22Rik. I would like to examine functional role of these lncRNAs and their target gene regulatory network in spermatogenesis.
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| Outline of Final Research Achievements |
Long non-conding RNAs (lncRNAs) are involved in the modulation of gene expression in various cellular processes including spermatogenesis. However, the expression of testis-specific lncRNAs and their functions are still unclear. The testis-exclusive lncRNAs had been identified previously and ISH results showed cell-specific expression of J01Rik lncRNA in spermatogenesis. In this study, I analyzed the expression patterns of lncRNAs during mouse testicular developmental periods and examined roles of testis-specific lncRNAs. I also examined the effects of arsenic exposure on J01Rik-expressed GC2 cells as environmental contaminants impact on reproduction and development.
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| Academic Significance and Societal Importance of the Research Achievements |
This study provides new information about a functional testis-specific lncRNA and its regulatory network in spermatogenesis. Newly-identified JO1Rik lncRNA was exclusively expressed in spermatocytes and it may be involved in regulation of cellular processes (cell cycle etc.)
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