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Analysis of molecular mechanisms regulating differentiation of taste bud cells using taste organoid culture system

Research Project

Project/Area Number 20K18461
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 57010:Oral biological science-related
Research InstitutionKyushu Dental College

Principal Investigator

Matsuyama Kae  九州歯科大学, 歯学部, 助教 (10848360)

Project Period (FY) 2020-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords味蕾 / オルガノイド / 分化 / Mash1
Outline of Research at the Start

QOLが重要視される現代において、味覚障害は注目を集める疾患のひとつである。高齢者では味蕾の数の減少が報告されているが、味蕾の維持や構成する細胞群の分化に関しては、不明な点が多い。本研究では、味蕾オルガノイドを活用し、舌上皮の幹細胞から味蕾細胞への分化プロセスにおける転写因子Mash1の役割を調べる。本研究により味蕾細胞の分化制御メカニズムを明らかにすることは味覚障害の病態解明につながると考える。

Outline of Final Research Achievements

We examined an Ascl1-expressing cell lineage using circumvallate papillae (CVP) of newborn mice and taste organoids, which allow studying the differentiation of taste bud cells in fine detail ex vivo. Using lineage-tracing analysis, we observed that Ascl1 lineage cells expressed type II and III taste cell markers both CVP of newborn mice and taste organoids. However, the coexpression rate in type II cells was lower than that in type III cells. Furthermore, we found that the generation of the cells which express type II and III cell markers was suppressed in taste organoids lacking Ascl1-expressing cells. These findings suggest that Ascl1-expressing precursor cells can differentiate into both type III and a subset of type II taste cells.

Academic Significance and Societal Importance of the Research Achievements

味蕾の発生、恒常性の維持には複数のシグナル伝達経路が関わることが報告されているものの、詳細なメカニズムは不明なままである。本研究により、転写因子Ascl1がIII型細胞だけでなく一部のII型細胞の分化過程で発現することが明らかになった。食生活は栄養学的側面だけでなく、精神的健康にも大きく影響する。QOLが重要視される現代において、味蕾の発生過程における生物学的プロセスを解き明かすことは、味覚障害の病態解明、さらには治療法の開発に貢献すると考える。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (8 results)

All 2023 2022 2021 2020

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (5 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Ascl1-expressing cell differentiation in initially developed taste buds and taste organoids2023

    • Author(s)
      Matsuyama Kae、Takai Shingo、Shigemura Noriatsu、Nakatomi Mitsushiro、Kawamoto Tatsuo、Kataoka Shinji、Toyono Takashi、Seta Yuji
    • Journal Title

      Cell and Tissue Research

      Volume: - Issue: 3 Pages: 631-641

    • DOI

      10.1007/s00441-023-03756-8

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mash1-expressing cells may be relevant to type III cells and a subset of PLCβ2-positive cell differentiation in adult mouse taste buds.2021

    • Author(s)
      Hsu CC, Seta Y, Matsuyama K, Kataoka S, Nakatomi M, Toyono T, Gunjigake KK, Kuroishi KN, Kawamoto T.
    • Journal Title

      Cell Tissue Res

      Volume: 383(2) Issue: 2 Pages: 667-675

    • DOI

      10.1007/s00441-020-03283-w

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Msx1 Heterozygosity in Mice Enhances Susceptibility to Phenytoin-Induced Hypoxic Stress Causing Cleft Palate2020

    • Author(s)
      Park Jinsil、Nakatomi Mitsushiro、Sasaguri Masaaki、Habu Manabu、Takahashi Osamu、Yoshiga Daigo、Matsuyama Kae、Kataoka Shinji、Toyono Takashi、Seta Yuji、Peters Heiko、Tominaga Kazuhiro
    • Journal Title

      The Cleft Palate-Craniofacial Journal

      Volume: - Issue: 6 Pages: 123-128

    • DOI

      10.1177/1055665620962690

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Differentiation of Ascl1-expressing precursor cells in taste buds2022

    • Author(s)
      Matsuyama, K., Kataoka, S., Toyono, T. and Seta, Y.
    • Organizer
      Asia-Pacific Conference in Fukuoka
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] Differentiation of Mash1-expressing precursor cells in taste buds2022

    • Author(s)
      Kae Matsuyama, Shinji Kataoka, Takashi Toyono, Yuji Seta
    • Organizer
      第64回歯科基礎医学会学術大会
    • Related Report
      2022 Research-status Report
  • [Presentation] The analysis of Mash1-expressing cell lineage in taste bud organoids2021

    • Author(s)
      Kae Matsuyama, Shinji Kataoka, Takashi Toyono, Yuji Seta
    • Organizer
      Asia-Pacific Conference in Fukuoka
    • Related Report
      2021 Research-status Report
  • [Presentation] 3D培養システムを用いた味細胞分化におけるMash1の機能解析2021

    • Author(s)
      松山 佳永, 川元 龍夫, 瀬田 祐司
    • Organizer
      第80回日本矯正歯科学会学術大会&第5回国際会議
    • Related Report
      2021 Research-status Report
  • [Presentation] Mash1 cell lineage analysis using taste bud organoid culture2021

    • Author(s)
      松山 佳永, 片岡 真司, 中富 満城, 豊野 孝, 瀬田 祐司
    • Organizer
      第126回日本解剖学会総会・全国学術集会/第98回日本生理学会大会 合同大会
    • Related Report
      2020 Research-status Report

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Published: 2020-04-28   Modified: 2025-01-30  

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