| Project/Area Number |
20K18989
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 58040:Forensics medicine-related
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| Research Institution | Fukuoka University |
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Principal Investigator |
Waters Brian 福岡大学, 医学部, 講師 (00609480)
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| Project Period (FY) |
2020-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2022: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | alcohol biomarker / ethyl glucoside / malic acid derivative / alcoholic beverages / GC-MS / forensic toxicology / forensic autopsy / forensic medicine / gas chromatography / mass spectrometry / malic acid ester / white wine / drinking markers / malic acid ethyl ester / 4-ethoxy-3-hydroxy / 4-oxobutanoic acid / Alcoholic beverages / Biomarkers / Volatiles / Fast gas chromatography / Tandem mass spectrometry / alcohol / biomarker / GC-MS/MS / SPME |
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| Outline of Research at the Start |
1) Identification of compounds present in beverages.
2) Comparison of analysis methods.
3) Testing of extraction methods to optimize the methods.
4) Optimization of instrument conditions.
5) Application of the methods to actual samples.
6) Interpretation of the results to the scientific community.
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| Outline of Final Research Achievements |
In this research, we searched for new biomarkers that can identify alcoholic beverage intake. After analyzing several alcoholic beverages and examining the urine of drinkers, we detected hordenine from beer drinkers, ethyl glucoside from beer, wine, and sake drinkers, a malic acid ester derivative from wine drinkers, and syringic acid from wine and whisky drinkers. Ethyl glucoside was detected at high concentrations specifically in sake drinkers. However, while not being present in whisky itself, it was also found in the urine of whisky drinkers, suggesting that it may be produced within the body. Further experiments confirmed that ethyl glucoside is produced in vitro from ethanol and sugars such as maltose. In the future, we will focus on the malic acid ester derivative by determining its exact chemical structure and by confirming its presence in various red and white wine varieties. We will also attempt to verify its potential as a specific biomarker for wine intake.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は、飲酒運転撲滅やアルコール依存症の診断・治療に貢献する新たな一歩を踏み出しました。従来の指標よりも長く体内に残る「エチルグルコシド」を飲酒運転の新指標として発見し、飲酒から時間が経過しても飲酒の有無を正確に判断できるようになりました。
さらに、この研究は、ワイン摂取量の客観的な指標開発にもつながり、個人の飲酒習慣把握やアルコール問題のリスク特定に役立つ可能性があります。ワイン中のリンゴ酸誘導体の存在も確認され、ワインの風味や品質への影響解明が期待されます。
本研究は、飲酒運転事故削減、アルコール依存症対策、ワイン品質評価向上など、社会に多大な貢献をもたらすことが期待されます。
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