Investigation of a novel analysis method for the determination of new biomarkers for alcoholic beverage consumption.
Project/Area Number |
20K18989
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 58040:Forensics medicine-related
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Research Institution | Fukuoka University |
Principal Investigator |
ウォーターズ ブライアン 福岡大学, 医学部, 講師 (00609480)
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Project Period (FY) |
2020-04-01 – 2024-03-31
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Project Status |
Granted (Fiscal Year 2022)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2022: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | alcohol biomarker / white wine / malic acid derivative / GC-MS / forensic toxicology / drinking markers / ethyl glucoside / malic acid ethyl ester / 4-ethoxy-3-hydroxy / 4-oxobutanoic acid / Alcoholic beverages / Biomarkers / Volatiles / Fast gas chromatography / Tandem mass spectrometry / alcohol / biomarker / GC-MS/MS / SPME |
Outline of Research at the Start |
1) Identification of compounds present in beverages. 2) Comparison of analysis methods. 3) Testing of extraction methods to optimize the methods. 4) Optimization of instrument conditions. 5) Application of the methods to actual samples. 6) Interpretation of the results to the scientific community.
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Outline of Annual Research Achievements |
In 2022, we focused on the analysis of a malic acid ester, which we had difficulty confirming the idenitity of because of a lack of a reference standard. This derivative was one of the compounds we focused on as a possible marker, particularly in white wine. We conducted a study where we analyzed urine samples from volunteers who consumed white wine, and we found that this derivative was present in most samples. In the coming year, we want to determine if the concentration of this derivative in urine correlates with the amount of white wine consumed. Also, is this derivative only present in white wine drinkers, or in other alcoholic beverages? Future research will explore its use of as a marker for white wine consumption and investigate its detection window.
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Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
In the first phase (2020) of this research, we were able to isolate compounds from alcoholic beverages that could act as drinking markers. In the second year (2021), we were able to eliminate compounds that were complicated to analyze, those that did not stay in the body longer than ethanol, and those that were not unique to alcohol consumption. Ethyl glucoside was idenitfied as a possible marker for the consuption of sake, and 2 original articles were published. In 2022, we turned our focus to a derivative of malic acid that appears to be present in the urine of white wine drinkers. Focusing on this one compound helps us to reliably develop an extraction and analysis method. Hopefully this work will continue to yield useful presentations and published papers.
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Strategy for Future Research Activity |
The project will continue with the optimization of the extraction and analysis methods for the malic acid derivative. Although no known reference standard is available, we can still optimize the extraction and analysis methods. This year we will look at alternate extraction methods like simplified liquid extraction (SLE) and QuEChERS. GC-MS(/MS) analysis and LC-MS/MS analysis will be using for analysis. Urine is the most efficient sample to use because of its ease of collection, but we hope to also expand to whole blood. Our hope to be able to make this a routine analysis that can any laboratory can easily add to their battery of tests. Now that COVID restrictions for conferences are loosening, we hope to be able to present and publish our results more readily.
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Report
(3 results)
Research Products
(15 results)