Nontarget screening for marine pollutants and elucidation of their biomagnification mechanisms

• Project/Area Number: 20K19984
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 64010:Environmental load and risk assessment-related
• Research Institution: Ehime University
• Principal Investigator: Goto Akitoshi 愛媛大学, 沿岸環境科学研究センター, 特定研究員 (90825689)
• Project Period (FY): 2020-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2021: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: ノンターゲットスクリーニング / GCxGC-HRToFMS / 有機ハロゲン化合物 / 生物濃縮 / 海洋生物

Outline of Research at the Start

海洋食物網を構成する低次-高次栄養段階生物を対象に、二次元ガスクロマトグラフ-高分解能飛行時間型質量分析計 (GCxGC-HRToFMS) 及びガスクロマトグラフ-高分解能二重収束型質量分析計 (GC-HRMS) を駆使したスクリーニング分析を実施することで、多様な有機ハロゲン化合物 (人工化学物質、海洋天然物質、構造・起源未知物質) の残留濃度や組成プロファイルの種差、そして生物濃縮機構の解明を試みる。

Outline of Final Research Achievements

The present study performed target/nontarget screening of marine pollutants in the environment and biota from Seto Inland Sea, Japan. The results of screening analyses revealed the bioaccumulation of various organohalogen compounds such as persistent organic pollutants (POPs), marine natural products, and structurally unknown compounds. Notably, several organochlorine compounds, identified as synthetic by-products or environmental/biological transformation products of dichlorodiphenyltrichloroethane (DDT) and chlordane (CHL), were found to accumulate in not only marine fish but also fish-eating birds. The results indicate chronic exposure of marine biota to these compounds, and their biomagnification potential in the marine food web.

Academic Significance and Societal Importance of the Research Achievements

本研究では、多次元クロマトグラフィーと精密質量分析を統合した分離計測技術を精緻化し、環境・生物汚染モニタリングに適用した結果、法的な監視や規制が及んでいない潜在的な有害物質群の検出に成功した。この成果は、応用計測科学や自然科学分野における研究の発展のみならず、自然環境や野生生物種の保全、そして化学物質の適正なリスク管理に資する有用な情報となり得る。

Research Products

• [Journal Article] Nontarget Screening of Organohalogen Compounds in the Liver of Wild Birds from Osaka, Japan: Specific Accumulation of Highly Chlorinated POP Homologues in Raptors (2021)
  • Author(s): Tue Nguyen Minh、Goto Akitoshi、Fumoto Mitsuo、Nakatsu Susumu、Tanabe Shinsuke、Kunisue Tatsuya
  • Journal Title: Environmental Science & Technology Volume: 55 Issue: 13 Pages: 8691-8699
  • DOI: 10.1021/acs.est.1c00357
  • Peer Reviewed / Open Access
• [Presentation] 海産の魚類を対象とした生物蓄積性有機ハロゲン化合物のサスペクトスクリーニング (2021)
  • Author(s): 後藤哲智, Nguyen Minh Tue, 田辺信介, 国末達也
  • Organizer: 第69回質量分析総合討論会
• [Presentation] 瀬戸内海の二枚貝と堆積物に残留する既知・未知有機ハロゲン化合物の生物濃縮性評価 (2021)
  • Author(s): 後藤哲智, Nguyen Minh Tue, 田辺信介, 国末達也
  • Organizer: 第29回環境化学討論会
• [Presentation] Evaluation of Biota-Sediment Accumulation Factors (BSAFs) for Known and Unknown Organohalogen Compounds in Japanese Mussels (2021)
  • Author(s): Goto, A., Tue, N. M., Tanabe, S. and Kunisue, T.
  • Organizer: 41th International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN 2021)
  • Int'l Joint Research
• [Presentation] Specific Accumulation of C15-Based Chlordane-Related Compounds in the Liver of Wild Birds from Japan (2021)
  • Author(s): Tue, N. M., Goto, A., Fumoto, M., Nakatsu, S., Tanabe, S. and Kunisue, T.
  • Organizer: 41th International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN 2021)
  • Int'l Joint Research