The establishment of a biomimetic human iPSC-derived cornea-on-a-chip for the pre-clinical evaluation of ophthalmic nanomedicines
| Project/Area Number |
20K20168
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 90110:Biomedical engineering-related
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| Research Institution | Ritsumeikan University (2021-2022)
Kyoto University (2020) |
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Principal Investigator |
ABDALKADER Rodi 立命館大学, 立命館グローバル・イノベーション研究機構, 助教授 (20839964)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2020: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | Cornea on a chip / Microfluidic / iPSC / hPSCs / Cornea-on-a-chip / Nano toxicity / corneal epithelial cells / pluripotent stem cells / microfluidics / Cornea / Nanomedicine |
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| Outline of Research at the Start |
This research envisions the establishment of a dynamic human cornea model through the interdisciplinary approach combining human induced pluripotent stem cells (iPSC) and microfluidics technologies. This platform will enable us to evaluate the pre-clinical efficacy and safety of the sophisticated ophthalmic nanoparticles that have the therapeutic potential in age-related eye diseases. Through that, we will be able to spatiotemporally evaluate the efficacy/toxicity and dynamic movement of nanomedicines.
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| Outline of Final Research Achievements |
The purpose of this research is to establish an in vitro model of the human cornea for studying the pre-clinical efficacy and toxicity of ophthalmic drugs. To achieve this, we have: (1) Employed microfluidic technology to construct a human corneal epithelium model that can be subjected to eye blinking-like stimuli.(2) Successfully generated functional corneal epithelial cells from human iPS cells. (3) Used untargeted metabolomics analysis to test the spatiotemporal evaluation of nutrient and metabolite transport and secretion in the human cornea on a chip.
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| Academic Significance and Societal Importance of the Research Achievements |
The development of a human cornea model that replicates eye-like blinking can enhance the prediction of ophthalmic drug safety and efficacy. Current preclinical models lack accuracy in this regard. This advancement enables the in vitro reproduction and study of the human cornea.
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Report
(4 results)
Research Products
(15 results)
