A mechanical study of lumen formation through membrane bleb regulation under hemodynamic influence
| Project/Area Number |
20K20190
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 90110:Biomedical engineering-related
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
MaungYe SweSoe 国立研究開発法人理化学研究所, 生命機能科学研究センター, 基礎科学特別研究員 (60866408)
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2022: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2021: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | Microhemodynamics / Hematocrit asymmetry / Wall shear stress / Vascular morphogenesis / Membrane blebbing / RBC mechanics / Endothelial mechanics / Vascular mechanics / Lumen formation / Angiogenesis / Vascular remodeling / Zebrafish |
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| Outline of Research at the Start |
Using zebrafish experiments, I will quantify the relationship between membrane blebbing and hemodynamics. The lumen morphogenesis model constructed from the quantification will study the mechanobiology of lumen formation as a spatiotemporal regulation between phenomena across vessel networks.
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| Outline of Final Research Achievements |
I established a method for calculating spatiotemporal distributions of blood pressure and wall shear stress (WSS) in a zebrafish microvascular network using experiment-based blood flow imaging and computational fluid dynamics (CFD).
With this technique I could study how pressure and WSS are regulated in zebrafish with different blood network geometry and different blood viscosity levels. In the experiment with blood cell removal, embryonic blood flow was observed to have a tendency to maintain blood flow rates in response to the blood viscosity reduction. Applying the CFD, we found that this scenario led to lowered network pressure and WSS. When vessel diameters were reduced by genetic mutation, blood flow rates were observed to be reduced in experiments. Using the CFD, it was indicated that both situations of network pressure maintenance or pressure reduction fit the flow rate reduction trend. Consequently, WSS in vessel reduction scenarios was either maintained.
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| Academic Significance and Societal Importance of the Research Achievements |
In situ blood viscosity, wall shear stress and blood pressure are difficult parameters to measure in microvessels. This combined methodology that employs both experiment and computational modeling provides a key tool for quantification of mechanical parameters involved in vascular morphogenesis.
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Report
(4 results)
Research Products
(5 results)
