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Analytical Chemistry at the Single Molecule Scale

Research Project

Project/Area Number 20K20458
Project/Area Number (Other) 19H05545 (2019)
Research Category

Grant-in-Aid for Challenging Research (Pioneering)

Allocation TypeMulti-year Fund (2020)
Single-year Grants (2019)
Review Section Medium-sized Section 43:Biology at molecular to cellular levels, and related fields
Research InstitutionKyoto University (2020-2023)
Institute of Physical and Chemical Research (2019)

Principal Investigator

Taniguchi Yuichi  京都大学, 高等研究院, 教授 (90556276)

Co-Investigator(Kenkyū-buntansha) 川井 隆之  国立研究開発法人理化学研究所, 生命機能科学研究センター, 客員研究員 (60738962)
金 水縁  京都大学, 高等研究院, 特定講師 (50758886)
大野 雅恵  京都大学, 高等研究院, 特定講師 (10581738)
Project Period (FY) 2020-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥26,000,000 (Direct Cost: ¥20,000,000、Indirect Cost: ¥6,000,000)
Fiscal Year 2022: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2021: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2020: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2019: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Keywords1分子蛍光検出 / 超高感度顕微鏡 / 分析化学 / 医療分析 / プロテオミクス / 1分子蛍光検出 / 1分子 / 蛍光イメージング / 生物物理学
Outline of Research at the Start

今日では、検体に含まれる分子の成分や含有量を調べるために、様々な蛍光検出を用いた分析化学の手法が開発されています。本研究では、1分子蛍光検出の原理を生体分子の検出・分析に適用することで、1分子粒度の分析化学の方法論を開拓することを目的とします。キャピラリ電気泳動などの様々な分析化学的手法への1分子蛍光検出系の導入を試み、その1分子感度化を目指します。

Outline of Final Research Achievements

We successfully enhanced various biochemical analytical methods such as ELISA and gel electrophoresis to single-molecule sensitivity, which is theoretically the highest level of sensitivity, based on our unique optics system. To achieve this, we performed developments of techniques such as an optical system, a liquid scanning system, an image analysis system, and a fluorescence labeling method. Furthermore, using these technologies, we performed high-throughput quantification of the number of various proteins contained in a single cell. The developed 'Single-Cell Proteome Profiling Method' enables the evaluation of a single cell's proteome affordably and conveniently. It holds promise for advancing in various life sciences research and clinical diagnostics that require quantification of rare molecules.

Academic Significance and Societal Importance of the Research Achievements

本研究の大きな意義の一つは、分析化学解析の感度を極限まで追求する点である。一般的なピペットマン操作でも扱えるマイクロリットルの液体内の分子の数を捉えられるため、アト・ゼプトモーラーレベルの極低濃度の検出を行うことが可能であり、一般的な蛍光検出法と比較すると感度が数億倍程度向上する。さらに、1分子粒度での解析ができることを利用して、単なる濃度情報だけではなく、各分子の細かな物性情報を解析することが可能であり、新たな分析化学の方法論の創出につながる。疾病の初期段階の診断や、多様な疾病の診断、低コスト化、侵襲性の低減化など、現代の分子医療診断の高度化に大きく貢献できるものと期待できる。

Report

(6 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Annual Research Report
  • Research Products

    (53 results)

All 2023 2022 2021 2020 2019 Other

All Int'l Joint Research (3 results) Journal Article (11 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 10 results,  Open Access: 6 results) Presentation (37 results) (of which Int'l Joint Research: 14 results,  Invited: 35 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 2 results)

  • [Int'l Joint Research] King’s College London(英国)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] ストラスブール大学(フランス)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] コペンハーゲン大学(デンマーク)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Recent methodological advances towards single-cell proteomics2023

    • Author(s)
      KIM Sooyeon、KAMARULZAMAN Latiefa、TANIGUCHI Yuichi
    • Journal Title

      Proceedings of the Japan Academy, Series B

      Volume: 99 Issue: 8 Pages: 306-327

    • DOI

      10.2183/pjab.99.021

    • ISSN
      0386-2208, 1349-2896
    • Year and Date
      2023-10-11
    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Single-Molecule Detection in the Study of Gene Expression2022

    • Author(s)
      Kumar, V., Leclerc, S., Taniguchi, Y.
    • Journal Title

      Single-Molecule Science: From Super-Resolution Microscopy to DNA Mapping and Diagnostics

      Volume: - Pages: 127-141

    • DOI

      10.1017/9781108525909.015

    • ISBN
      9781108525909, 9781108423366, 9781108437479
    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Hi-CO: 3D genome structure analysis with nucleosome resolution2021

    • Author(s)
      Ohno Masae、Ando Tadashi、Priest David G.、Taniguchi Yuichi
    • Journal Title

      Nature Protocols

      Volume: 16 Issue: 7 Pages: 3439-3469

    • DOI

      10.1038/s41596-021-00543-z

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] iNucs: inter-nucleosome interactions2021

    • Author(s)
      Oveisi Mehrdad、Shukla Manu、Seymen Nogayhan、Ohno Masae、Taniguchi Yuichi、Nahata Sunil、Loos Remco、Mufti Ghulam J、Allshire Robin C、Dimitrov Stefan、Karimi Mohammad M
    • Journal Title

      Bioinformatics

      Volume: 37 Issue: 23 Pages: 4562-4563

    • DOI

      10.1093/bioinformatics/btab698

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] PHi-C: deciphering Hi-C data into polymer dynamics2020

    • Author(s)
      Shinkai Soya、Nakagawa Masaki、Sugawara Takeshi、Togashi Yuichi、Ochiai Hiroshi、Nakato Ryuichiro、Taniguchi Yuichi、Onami Shuichi
    • Journal Title

      NAR Genomics and Bioinformatics

      Volume: 2 Issue: 2

    • DOI

      10.1093/nargab/lqaa020

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 単一細胞レベル解析の新技術2020

    • Author(s)
      谷口 雄一・大野 雅恵
    • Journal Title

      月刊「細胞」2020年 11月号

      Volume: 52 Pages: 741-745

    • Related Report
      2020 Research-status Report
  • [Journal Article] A top-down algorithm for identifying the multi-scale hierarchical structure of chromosomes2020

    • Author(s)
      Kumar, V., Leclerc, S., Taniguchi, Y.
    • Journal Title

      Nucleic Acids Research

      Volume: 48 Issue: 5 Pages: e26-e26

    • DOI

      10.1093/nar/gkaa004

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Simultaneous Single-Cell Measurements Demonstrate a Positive Correlation between RNA Copy Number for Mitochondrial Division and Fusion Genes and Mitochondrial Fragmentation2019

    • Author(s)
      Yoshida Yamato、Taniguchi Yuichi
    • Journal Title

      CYTOLOGIA

      Volume: 84 Issue: 1 Pages: 15-23

    • DOI

      10.1508/cytologia.84.15

    • NAID

      130007628777

    • ISSN
      0011-4545, 1348-7019
    • Year and Date
      2019-03-25
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Simultaneous Measurements of RNA Molecules Transcribed from Mitochondrial Division or Fusion Genes in Single Cells2019

    • Author(s)
      Yoshida Yamato、Taniguchi Yuichi
    • Journal Title

      CYTOLOGIA

      Volume: 84 Issue: 1 Pages: 1-1

    • DOI

      10.1508/cytologia.84.1

    • NAID

      130007628776

    • ISSN
      0011-4545, 1348-7019
    • Year and Date
      2019-03-25
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level2019

    • Author(s)
      Leclerc Simon、Arntz Youri、Taniguchi Yuichi
    • Journal Title

      Journal of Visualized Experiments

      Volume: 146 Issue: 146

    • DOI

      10.3791/59199

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Resolving 3D Higher-order Molecular Structure of the Genome2019

    • Author(s)
      谷口 雄一, 大野 雅恵
    • Journal Title

      Seibutsu Butsuri

      Volume: 59 Issue: 6 Pages: 305-309

    • DOI

      10.2142/biophys.59.305

    • NAID

      130007751042

    • ISSN
      0582-4052, 1347-4219
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Unravelling genome structure at the nucleosome level with Hi-CO2023

    • Author(s)
      大野雅恵
    • Organizer
      第3回 Hi-C研究会
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] Ultrasensitive bioanalyses via 3D single-molecule imaging2023

    • Author(s)
      Sooyeon Kim
    • Organizer
      iCeMS & NCKU Biliteral symposium
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 分子解像度での生命理解に向けて2023

    • Author(s)
      谷口雄一
    • Organizer
      第7回IPRフロンティアセミナー
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] 一分子電気泳動法の開発と展開2023

    • Author(s)
      金 水縁、Latiefa Kamarulzaman、谷口 雄一
    • Organizer
      第45回日本光医学・光生物学会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 一分子蛍光電気泳動の開発とその応用2023

    • Author(s)
      金 水縁、Latiefa Kamarulzaman、谷口 雄一
    • Organizer
      第23回日本光生物学会協会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] ヌクレオソーム分解能での ゲノムの3次元構造解析2023

    • Author(s)
      谷口雄一
    • Organizer
      第2回Philosophy研究会
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] Nucleosome-level 3D organization of the genome2023

    • Author(s)
      Taniguchi, Y
    • Organizer
      16th International Symposium on Nanomedicine (ISNM2023)
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] Ultrasensitive bioanalyses based on single-molecule microscopic imaging2023

    • Author(s)
      Sooyeon Kim
    • Organizer
      25th iCeMS International Symposium
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 3D molecular structure analysis of the genome by coupling next-generation DNA sequencing and molecular dynamics simulation2022

    • Author(s)
      Taniguchi, Y.
    • Organizer
      The 45th Annual Meeting of the Molecular Biology Society of Japan
    • Related Report
      2022 Research-status Report
    • Invited
  • [Presentation] Nucleosome-level 3D organization of the genome2022

    • Author(s)
      Taniguchi, Y.
    • Organizer
      Huazhong University of Science and Technology Lecture
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] シングルセルにおける遺伝子発現ダイナミクスの定量化2022

    • Author(s)
      谷口雄一
    • Organizer
      第9回日本リウマチ学会ベーシックリサーチカンファレンス
    • Related Report
      2022 Research-status Report
    • Invited
  • [Presentation] 1分子イメージング法を利用した超高感度生化学分析法の開発2022

    • Author(s)
      谷口雄一
    • Organizer
      学術変革領域研究(B)「機能性ラマンプローブによる革新的多重イメージング」オンラインセミナー
    • Related Report
      2022 Research-status Report
    • Invited
  • [Presentation] Nucleosome-level 3D organization of the genome2022

    • Author(s)
      Taniguchi, Y.
    • Organizer
      15th Asia-Pacific Physics Conference (APPC15)
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Nucleosome-level 3D organization of the genome2022

    • Author(s)
      Taniguchi, Y
    • Organizer
      2nd WPI NanoLSI-iCeMS Joint Symposium
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 分子解像度での生命理解に向けて2022

    • Author(s)
      谷口雄一
    • Organizer
      東大物性研ワークショップ:開放系トポロジーと生体・量子・統計物理
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] Nucleosome-level 3D organization of the genome2021

    • Author(s)
      Taniguchi, Y
    • Organizer
      Pacifichem 2021
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 1分子検出に基づく次世代型生化学分析法の開発2021

    • Author(s)
      谷口雄一
    • Organizer
      ARO協議会第8回学術集会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] Nucleosome-level 3D organization of the genome2021

    • Author(s)
      Taniguchi, Y
    • Organizer
      CMUH-iCeMS Joint Meeting
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Nucleosome-level 3D organization of the yeast genome2021

    • Author(s)
      Taniguchi, Y
    • Organizer
      Cold Spring Harbor Asia Conference: Yeast and life science
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 1分子感度の生化学分析技術の開発2021

    • Author(s)
      谷口雄一
    • Organizer
      AMED難治性疾患実用化研究事業2020年度成果報告会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] 1分子感度での生化学分析2021

    • Author(s)
      谷口雄一
    • Organizer
      第75回日本口腔科学会学術集会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] Molecular-resolved biology for understanding complex Biosystems2021

    • Author(s)
      Taniguchi, Y
    • Organizer
      iCeMS-MacDiarmid Institute Online Workshop
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Molecular-resolved biology for understanding complex biosystems2021

    • Author(s)
      Yuichi Taniguchi
    • Organizer
      iCeMS-MacDiarmid Institute Online Workshop
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Revealing nucleosome-resolved 3D genome architecture2021

    • Author(s)
      Yuichi Taniguchi
    • Organizer
      RIKEN BDR Symposium 2021
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Nucleosome-level 3D organization of the genome2020

    • Author(s)
      Taniguchi, Y.
    • Organizer
      RIKEN Life Science Retreat, Shizuoka, Japan
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] ゲノムの3次元高次分子構造を解く2020

    • Author(s)
      谷口雄一
    • Organizer
      東京大学生物科学セミナー、東京大学
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Nucleosome-level 3D organization of the genome2020

    • Author(s)
      Taniguchi, Y.
    • Organizer
      2020 POSTECH-APCTP Symposium, Pohang, Korea
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Nucleosome-level 3D organization of the genome2020

    • Author(s)
      Taniguchi, Y.
    • Organizer
      Seoul National University Seminar, Seoul, Korea
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ヌクレオソーム配列構造による遺伝子制御2019

    • Author(s)
      谷口雄一
    • Organizer
      第42回日本分子生物学会ワークショップ、福岡国際会議場
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 3D nucleosome-resolution genome structure analysis2019

    • Author(s)
      Taniguchi, Y.
    • Organizer
      14th Asia-Pacific Physics Conference (APPC14), Kuching, Malaysia,
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ヌクレオソーム分解能でのゲノム3次元構造の定量解析2019

    • Author(s)
      谷口雄一
    • Organizer
      定量生物学の会、北海道大学
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 1分子電気泳動解析2019

    • Author(s)
      谷口雄一
    • Organizer
      第69回日本電気泳動学会シンポジウム、慶應大学三田キャンパス
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] ヌクレオソーム分解能での3次元ゲノム構造解析2019

    • Author(s)
      谷口雄一
    • Organizer
      第57回日本生物物理学会シンポジウム、宮崎シーガイアコンベンションセンター
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] ゲノム DNA の3次元分子構造解析技術2019

    • Author(s)
      谷口雄一
    • Organizer
      大阪大学蛋白質研究所セミナー
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 細胞内でゲノムはどのような構造で収納されているか?2019

    • Author(s)
      谷口雄一
    • Organizer
      第31回高遠分子細胞生物学シンポジウム、高遠さくらホテル
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 分子レベルでの生命理解のための新技術2019

    • Author(s)
      谷口雄一
    • Organizer
      先端的バイオ計測法研究会、東京大学
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 1分子感度での『分子診断法』の開発2019

    • Author(s)
      谷口雄一
    • Organizer
      第63回理研イブニングセミナー、理研三宮拠点
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Patent(Industrial Property Rights)] 顕微鏡、焦準器具、流体保持器具、及び光学ユニット2019

    • Inventor(s)
      谷口 雄一、西村 和哉
    • Industrial Property Rights Holder
      理化学研究所
    • Industrial Property Rights Type
      実用新案
    • Filing Date
      2019
    • Related Report
      2019 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 顕微鏡、焦準器具、流体保持器具、及び光学ユニット2019

    • Inventor(s)
      谷口 雄一、西村 和哉
    • Industrial Property Rights Holder
      理化学研究所
    • Industrial Property Rights Type
      実用新案
    • Filing Date
      2019
    • Acquisition Date
      2019
    • Related Report
      2019 Annual Research Report
    • Overseas

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Published: 2019-07-04   Modified: 2025-01-30  

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