Why can endophytic commensal bacteria colonize plants by evading the host immunity mechanism?

• Project/Area Number: 20K20572
• Research Category: Grant-in-Aid for Challenging Research (Pioneering)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 39:Agricultural and environmental biology and related fields
• Research Institution: Okayama University
• Principal Investigator: Noutoshi Yoshiteru 岡山大学, 学術研究院環境生命科学学域, 准教授 (70332278)
• Co-Investigator(Kenkyū-buntansha): 川口 章 国立研究開発法人農業・食品産業技術総合研究機構, 西日本農業研究センター, 上級研究員 (80520486)
• Project Period (FY): 2020-07-30 – 2023-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥26,000,000 (Direct Cost: ¥20,000,000、Indirect Cost: ¥6,000,000); Fiscal Year 2022: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000); Fiscal Year 2021: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2020: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
• Keywords: ブドウ根頭がんしゅ病 / バイオコントロール / 微生物農薬 / 片利共生微生物 / 共生 / 寄生 / Rhizobium属細菌 / 植物微生物相互作用 / リゾビウム属細菌 / 片利共生細菌 / 植物根圏微生物 / 宿主定着能 / 植物免疫 / エンドファイト / 植物生育促進細菌 / 片利共生 / バイオコントロール細菌 / 植物 / 定着

Outline of Research at the Start

植物は微生物を感知して自然免疫系を誘導し、それらを排除する精緻な防御システムを持つことが明らかになってきた。一方、植物の内部には根粒菌や菌根菌のような相利共生微生物に加え、エンドファイトと呼ばれる片利共生微生物が常在する。ではこれらの共生微生物はどのように宿主の免疫機構を回避しているのだろうか。本研究では、「植物に共生できる微生物は利益供与の有無に依らず、宿主の防御機構を回避する手段や能力を持つ」と仮定し、その証明からメカニズムの解明に迫る。具体的には、ブドウ根頭がんしゅ病を抑制するバイオコントロール細菌を用い、その宿主定着特性を失う変異体の単離とその解析を実施する。

Outline of Final Research Achievements

Grapevine crown gall disease is caused by Allorhizobium vitis and can be suppressed by A. vitis VAT03-1 and ARK-1. Using these antagonist bacteria as materials, we aimed to elucidate the mechanism by which commencal bacteria establish mutualistic interaction with host plants to provide a basis for the proper utilization of biocontrol agents. This study showed that plants exude carbon produced by photosynthesis from their roots and deliver it to the soil, and the bacteria grow and colonize plant roots by using plant-derived carbon sources and inorganic substances around the roots. The bacteria then return soil nutrients to their hosts from a spontaneously dead population. The results also suggest that the immune priming effect of the colonized bacteria in the host plant may contribute to some extent to disease suppression.

Academic Significance and Societal Importance of the Research Achievements

菌根菌は土壌から無機成分を吸収して宿主に供給し、その代わりに光合成産物を得る相利共生糸状菌である。植物根圏に定着する細菌は、一般に植物由来の炭素源を得るのみの片利共生微生物と認識されるが、一定条件下において、菌根菌よりは極めて範囲ではあるが、根周辺から無機成分を集めて供給し、その生長を促進する相利共生の側面を持つことが示唆された。1対1のシンプルな解析系でこそ得られた知見と考えている。根に集積した細菌には、病原菌に拮抗活性を持つものや、植物の免疫応答を強めるものがあることから、植物-微生物の相互作用を制御することで、作物の生長制御や病害抑制といった農業利用への展開が期待される。

Research Products

• [Int'l Joint Research] Virginia Tech(米国)
• [Int'l Joint Research] Virginia Tech(米国)
• [Journal Article] The function of the plant cell wall in plant?microbe interactions (2022)
  • Author(s): Ishida Konan, Noutoshi Yoshiteru
  • Journal Title: Plant Physiology and Biochemistry Volume: 192 Pages: 273-284
  • DOI: 10.1016/j.plaphy.2022.10.015
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Insight into inducing disease resistance with Allorhizobium vitis strain ARK-1, a biological control agent against grapevine crown gall disease (2022)
  • Author(s): Akira Kawaguchi, Yoshiteru Noutoshi
  • Journal Title: European Journal of Plant Pathology Volume: 162 Issue: 4 Pages: 981-987
  • DOI: 10.1007/s10658-021-02440-3
  • Peer Reviewed
• [Journal Article] Origin of pathogens of grapevine crown gall disease in Hokkaido in Japan as characterized by molecular epidemiology of Allorhizobium vitis strains (2021)
  • Author(s): Akira Kawagushi, Teruo Sone, Sunao Ochi, Yosuke Matsushita, Yoshiteru Noutoshi, Mizuho Nita
  • Journal Title: Life Volume: 11 Issue: 11 Pages: 1265-1265
  • DOI: 10.3390/life11111265
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Migration of biological control agent Rhizobium vitis strain ARK-1 in grapevine stems and inhibition of galls caused by tumorigenic strain of R. vitis (2021)
  • Author(s): Akira Kawaguchi, Yoshiteru Noutoshi
  • Journal Title: Journal of General Plant Pathology Volume: 88 Issue: 1 Pages: 63-68
  • DOI: 10.1007/s10327-021-01043-4
  • Peer Reviewed
• [Journal Article] Complete Genome Sequence Data of Tumorigenic Rhizobium vitis Strain VAT03-9, a Causal Agent of Grapevine Crown Gall Disease (2020)
  • Author(s): Yoshiteru Noutoshi, Atsushi Toyoda, Tomoya Ishii, Kirara Saito, Megumi Watanabe, Akira Kawaguchi
  • Journal Title: Molecular Plant-Microbe Interactions® Volume: 33 Issue: 11 Pages: 1280-1282
  • DOI: 10.1094/mpmi-07-20-0180-a
  • NAID: 120007004826
  • Peer Reviewed / Open Access
• [Journal Article] Complete Genome Sequence Data of Nonpathogenic and Nonantagonistic Strain of Rhizobium vitis VAR06-30 Isolated From Grapevine Rhizosphere (2020)
  • Author(s): Yoshiteru Noutoshi, Atsushi Toyoda, Tomoya Ishii, Kirara Saito, Megumi Watanabe, Akira Kawaguchi
  • Journal Title: Molecular Plant-Microbe Interactions® Volume: 33 Issue: 11 Pages: 1283-1285
  • DOI: 10.1094/mpmi-07-20-0182-a
  • NAID: 120007004828
  • Peer Reviewed / Open Access
• [Journal Article] Complete Genome Sequence Data of Nonpathogenic Strain Rhizobium vitis VAR03-1, a Biological Control Agent for Grapevine Crown Gall Disease (2020)
  • Author(s): Yoshiteru Noutoshi, Atsushi Toyoda, Tomoya Ishii, Kirara Saito, Megumi Watanabe, Akira Kawaguchi
  • Journal Title: Molecular Plant-Microbe Interactions® Volume: 33 Issue: 12 Pages: 1451-1453
  • DOI: 10.1094/mpmi-07-20-0181-a
  • NAID: 120007004827
  • Peer Reviewed / Open Access
• [Presentation] The impact of Rhizobium vitis VAR03-1 on the growth of Arabidopsis thaliana in different nutrient conditions. (2023)
  • Author(s): Hemelda N.M., Bao J., Matsui H., Yamamoto M., Toyoda K., Ichinose Y., Noutoshi, Y.
  • Organizer: 令和5年度日本植物病理学会大会
• [Presentation] The effect of sucrose on the interaction between Rhizobium vitis VAR03-1 and Arabidopsis thaliana. (2022)
  • Author(s): Hemelda N.M., Bao J., Matsui H., Yamamoto M., Toyoda K., Ichinose Y., Noutoshi, Y.
  • Organizer: Plant Microbe Research Network(PMRN) 第２回オンラインシンポジウム
• [Presentation] The effect of sucrose on the interaction between Rhizobium vitis VAR03-1 and Arabidopsis thaliana. (2022)
  • Author(s): Hemelda N.M., Bao J., Matsui H., Yamamoto M., Toyoda K., Ichinose Y., Noutoshi, Y.
  • Organizer: 令和4年度日本植物病理学会関西部会
• [Presentation] Supplementation of Sucrose Disrupts Mutual Interaction between Rhizobium vitis VAR03-1, a Commensal Bacterial Strain, and Arabidopsis thaliana. (2022)
  • Author(s): Hemelda N.M., Bao J., Matsui H., Yamamoto M., Toyoda K., Ichinose Y., Noutoshi, Y.
  • Organizer: JSOL2022
• [Presentation] Rhizobium vitis VAR03-1 株のブドウ根頭がんしゅ病拮抗能を司るテイロシン rhizoviticin の機能解析 (2022)
  • Author(s): 土田菜月, 石井智也, 渡邉恵, 齊藤晶, 包継源, Niarsi Merry Hemelda, 佐藤繭子, 豊岡公徳, 石濱伸明, 白須賢, 豊田敦, 松原岳大, 松井英譲, 山本幹博, 豊田和弘, 一瀬勇規, 川口章, 能年義輝
  • Organizer: 令和４年度日本植物病理学会大会
• [Presentation] ブドウ根頭がんしゅ病拮抗細菌を用いた片利共生細菌のシロイヌナズナ根圏への定着能の解析 (2022)
  • Author(s): 包継源, Niarsi Merry Hemelda, 土田菜月, 渡邉恵, 松井英譲, 山本幹博, 豊田和弘, 一瀬勇規, 川口章, 能年義輝
  • Organizer: 令和４年度日本植物病理学会大会
• [Presentation] Characterization of rhizoviticin, a phage tail-like particle, as a determinant of biocontrol activity of Rhizobium vitis VAR03-1 on crown gall disease of grapevine (2021)
  • Author(s): Tsuchida, N., Ishii, T., Watanabe, M., Saito, K., Bao, J., Sato, M., Toyooka, K., Ishihama, N., Shirasu, K., Toyoda, A., Matsubara, T., Matsui, H., Yamamoto, M., Ichinose, Y., Toyoda, K., Kawaguchi, A., Noutoshi, Y.
  • Organizer: Plant Microbe Research Network(PMRN) 第1回オンラインシンポジウム
• [Presentation] Colonization mechanism of Rhizobium vitis VAR03-1, a biocontrol bacterial agent for grapevine crown gall disease, in Arabidopsis thaliana (2021)
  • Author(s): Bao, J., Matsui, H., Yamamoto, M., Ichinose, Y., Toyoda, K., Kawaguchi, A., Noutoshi, Y.
  • Organizer: Plant Microbe Research Network(PMRN) 第1回オンラインシンポジウム
• [Presentation] Rhizobium vitis VAR03-1 株のブドウ根頭がんしゅ病拮抗能を司るテイロシン rhizoviticin の機能解析 (2021)
  • Author(s): 土田菜月, 石井智也, 渡邉恵, 齊藤晶, 包継源, 佐藤繭子, 豊岡公徳, 石濱伸明, 白須賢, 豊田敦, 松原岳大, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 川口章, 能年義輝
  • Organizer: 令和３年度植物感染生理談話会
• [Presentation] ブドウ根頭がんしゅ病拮抗細菌を用いた片利共生細菌のシロイヌナズナ根圏への定着能の解析 (2021)
  • Author(s): 包継源, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 川口章, 能年義輝
  • Organizer: 令和３年度植物感染生理談話会
• [Presentation] Characterization of rhizoviticin, a phage tail-like particle, as a determinant of biocontrol activity of Rhizobium vitis VAR03-1 on crown gall disease of grapevine (2021)
  • Author(s): Tsuchida, N., Ishii, T., Watanabe, M., Saito, K., Bao, J., Sato, M., Toyooka, K., Ishihama, N., Shirasu, K., Toyoda, A., Matsubara, T., Matsui, H.,Yamamoto, M., Ichinose, Y., Toyoda, K., Kawaguchi, A., Noutoshi, Y.
  • Organizer: IPSR Plant Web Forum 2021
  • Int'l Joint Research
• [Presentation] Colonization mechanism of Rhizobium vitis VAR03-1, a biocontrol bacterial agent for grapevine crown gall disease, in Arabidopsis thaliana (2021)
  • Author(s): Bao, J., Matsui, H., Yamamoto, M., Ichinose, Y., Toyoda, K., Kawaguchi, A., Noutoshi, Y.
  • Organizer: IPSR Plant Web Forum 2021
  • Int'l Joint Research
• [Presentation] Rhizobium vitis VAR03-1 株のブドウ根頭がんしゅ病拮抗能を司るテイロシン rhizoviticin の機能解析 (2021)
  • Author(s): 土田菜月, 石井智也, 渡邉恵, 齊藤晶, 包継源, 佐藤繭子, 豊岡公徳, 石濱伸明, 白須賢, 豊田敦, 松原岳大, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 川口章, 能年義輝
  • Organizer: ウイルス学若手研究集会2021
• [Presentation] ブドウ根頭がんしゅ病拮抗細菌を用いた片利共生細菌のシロイヌナズナ根圏への定着能の解析 (2021)
  • Author(s): 包継源, 松井英譲, 山本幹博, 一瀬勇規, 豊田和弘, 川口章, 能年義輝
  • Organizer: ウイルス学若手研究集会2021