生物応用に向けた常温・常圧・水中ではたらく合成分子モーターの設計と合成
| Project/Area Number |
20K21131
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 28:Nano/micro science and related fields
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
ラッペン ゲナエル 奈良先端科学技術大学院大学, 先端科学技術研究科, 教授 (60812576)
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| Co-Investigator(Kenkyū-buntansha) |
安原 主馬 奈良先端科学技術大学院大学, 先端科学技術研究科, 准教授 (90545716)
西野 智雄 奈良先端科学技術大学院大学, 先端科学技術研究科, 助教 (60824878)
尾本 賢一郎 奈良先端科学技術大学院大学, 先端科学技術研究科, 特任助教 (40820056)
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| Project Period (FY) |
2020-07-30 – 2024-03-31
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| Project Status |
Granted (Fiscal Year 2022)
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| Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2022: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2021: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2020: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | Molecular motor / Amphiphile / fluorescent tag / single molecule / membrane insertion / 分子マシン / 分子モーター / 超分子 / テクノミメティクス / 生物活性 |
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| Outline of Research at the Start |
本研究課題では、研究代表者らがこれまでに開発した単一方向にのみ回転できる合成分子モーターの力学的作用を利用した生物活性の誘導・制御に挑戦する。具体的には、細胞膜に作用する分子モーターを新たに設計・合成し、その回転によってもたらされる膜構造や物性の攪乱について、物理化学的および生化学的手法により評価を行うことで、分子スケールでのミクロな力学に基づいた新しい生物活性分子の提案を目指す。
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| Outline of Annual Research Achievements |
The synthesis of the new target has been advanced with the formation of the amphiphilic molecular motor functionalized with a chemical tag. As tag, we developed the synthesis of a rhodamine fragment which is very classic to be observed by optical microscopes.
Now, just one chemical step is missing to connect the amphiphilic motor with long alkyl chains and the rhodamine fluorescent derivative needed to follow the molecule in the artificial membrane. The coupling was not efficient yet so new connection strategies will be pursued in the next year.
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| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
The project has been slowed down due to the COVID-19 sanitary crisis but we plan to finish the synthesis of the final compound as soon as possible before testing its integration in artificial membranes hopefully in the middle of the next year.
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| Strategy for Future Research Activity |
The synthesis of the target is not finished yet. We need to pursue our effort in its synthesis and characterization. After the synthesis will be achieved, the targeted amphiphilic motor will be fully characterized, including the absorption and emission properties necessary for its detection inside of an artificial membrane.
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Report
(3 results)
Research Products
(18 results)
