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Elucidation of the mechanisms by which endobacteria give symbiotic ability to Mortierella spp.

Research Project

Project/Area Number 20K21301
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 39:Agricultural and environmental biology and related fields
Research InstitutionIbaraki University

Principal Investigator

Narisawa Kazuhiko  茨城大学, 農学部, 教授 (90431650)

Co-Investigator(Kenkyū-buntansha) 晝間 敬  東京大学, 大学院総合文化研究科, 准教授 (20714504)
西澤 智康  茨城大学, 農学部, 准教授 (40722111)
Project Period (FY) 2020-07-30 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2021: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2020: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Keywords内生細菌 / Mortierella属菌 / 共生 / 植物生育促進 / 植物共生
Outline of Research at the Start

本研究は、Mortierella属菌を対象として、内生する細菌との相互作用を解明し、腐生菌を共生菌に変え、作物生産に利用する革新的な挑戦である。我々が菌類として認識していた生物が、いわば菌類と細菌の共生体であるという、今までの菌学の概念を覆し、新たな生物共生系の存在を示す強いインパクトをもたらす。成果として①内生細菌は複数系統存在する②内生細菌の違いにより、共生や腐生を示すことが明らかとなることが期待される。さらに、宿主菌類と内生細菌の組み合わせにより、新たな有用物質の生産など、菌類の諸形質を制御することが可能となる。また、植物の生産性や品質向上などの利点をもたらすことにもつながる。

Outline of Final Research Achievements

We succeeded in culturing Mycoavidus sp. S2-EB, which is the BRE of Mortierella humilis S2. Mycoavidus sp. S2-EB was found to carry 2,575,677 bp chromosomes and 99,085 bp plasmids.
Confrontation culture of M. verticillata YTM181BF1 + Mycoavidus sp. S2-EB was performed on LCA medium. Originally, the BRE endogenous to M. verticillata YTM181 belongs to Group C, but what was detected after the confrontation culture was the BRE belonging to Group A used in the reintroduction method.
This result indicates the possibility of successful reintroduction of BRE into Mortierella spp.

Academic Significance and Societal Importance of the Research Achievements

本研究では、菌類-内生細菌を共生系としてとらえ、内生細菌を操る手法の開発を目的としている。この技術は、菌類の腐生性を共生に変え作物生産へ利用することへつながる。我々が菌類として認識していた生物が、いわば菌類と細菌の共生体であるという、これまでの菌類学の概念を覆し、新たな生物共生系の存在を示すことが可能となる。

Report

(3 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • Research Products

    (7 results)

All 2022 2021 2020

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 2 results) Presentation (3 results) (of which Invited: 1 results)

  • [Journal Article] Whole-Genome Sequence of Entomortierella parvispora E1425, a Mucoromycotan Fungus Associated with Burkholderiaceae-Related Endosymbiotic Bacteria2022

    • Author(s)
      Herlambang Afri、Guo Yong、Takashima Yusuke、Narisawa Kazuhiko、Ohta Hiroyuki、Nishizawa Tomoyasu
    • Journal Title

      Microbiology Resource Announcements

      Volume: 11 Issue: 1 Pages: 13-13

    • DOI

      10.1128/mra.01101-21

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The Effectiveness of a Dark Septate Endophytic Fungus, Cladophialophora chaetospira SK51, to Mitigate Strawberry Fusarium Wilt Disease and With Growth Promotion Activities2020

    • Author(s)
      Harsonowati Wiwiek、Marian Malek、Surono、Narisawa Kazuhiko
    • Journal Title

      Frontiers in Microbiology

      Volume: 11 Pages: 585-585

    • DOI

      10.3389/fmicb.2020.00585

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Aposymbiosis of a <i>Burkholderiaceae</i>-Related Endobacterium Impacts on Sexual Reproduction of Its Fungal Host2020

    • Author(s)
      Takashima Yusuke、Degawa Yousuke、Nishizawa Tomoyasu、Ohta Hiroyuki、Narisawa Kazuhiko
    • Journal Title

      Microbes and Environments

      Volume: 35 Issue: 2 Pages: n/a

    • DOI

      10.1264/jsme2.ME19167

    • NAID

      130007831730

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mycoavidus sp. Strain B2-EB: Comparative Genomics Reveals Minimal Genomic Features Required by a Cultivable Burkholderiaceae-Related Endofungal Bacterium2020

    • Author(s)
      Guo Yong、Takashima Yusuke、Sato Yoshinori、Narisawa Kazuhiko、Ohta Hiroyuki、Nishizawa Tomoyasu
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 86 Issue: 18 Pages: 18-20

    • DOI

      10.1128/aem.01018-20

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Presentation] クサレケカビ属菌における菌類-細菌共生体に関する研究2021

    • Author(s)
      高島勇介
    • Organizer
      日本菌学会第 65 回大会日本菌学会奨励賞
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 有機農法水田に自生する植物に定着する菌類叢の解析2020

    • Author(s)
      関根直人、成澤才彦
    • Organizer
      日本土壌微生物学会 2020年度大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 高温ストレス条件下でのVeronaeopsis simplex Y34接種トマトのHeat Shock Proteinの発現状態2020

    • Author(s)
      草谷奈津子、晝間敬、成澤才彦
    • Organizer
      日本土壌微生物学会 2020年度大会
    • Related Report
      2020 Research-status Report

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Published: 2020-08-03   Modified: 2023-01-30  

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