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Mechanism of insect cannibalism via chemical component of cuticular wax

Research Project

Project/Area Number 20K21304
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 39:Agricultural and environmental biology and related fields
Research InstitutionThe University of Tokyo

Principal Investigator

Nagata Shinji  東京大学, 大学院新領域創成科学研究科, 教授 (40345179)

Project Period (FY) 2020-07-30 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2022: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2021: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords昆虫 / 共食い / 体表ワックス / 雑食性
Outline of Research at the Start

フタホシコオロギを用いて、「共食い」の生理学的、栄養学的な条件を検討した結果、栄養分依存的かつパンデミック抑止的に「共食い」が起こることを見出した。また、体表ワックスの成分が「共食い」を抑制的に機能していることも見出した。そこで、本研究では、フタホシコオロギを用いて、①健康状態とCHCの組成の変化を分析し、②体力的に弱者のCHCの生合成遺伝子の変動について検討する。申請期間内には、CHC成分変動による被捕食者となる確率が上がる原因として、フタホシコオロギの代謝系、栄養状態などがCHC生合成と関連していることを突き止め、最終的にはフタホシコオロギでみられる共食いの分子メカニズムの解明に迫る。

Outline of Final Research Achievements

In this study, we pursued the relationship between the cannibalism and the composition of cuticular wax (cuticular hydrocarbon; CHC) in the two-spotted cricket. The enzymes involved in CHC biosynthetic pathway (FAS, Desat, ELOVL, and P450) were identified. These enzymes had several subtypes, and then we examine currently whether they are involved in CHC.
In addition, aggressive and cannibalistic behaviors, which were thought to be observed mainly in adults, were found to be observed at any time in the life cycle in crickets. In particular, we were able to efficiently reproduce cannibalistic behavior during the nyphal stage due to differences in body size. If this cannibalistic behavior in the larval stage can be used as a bioassay, our researches on the relationship between cannibalism and CHC will be further progressed.

Academic Significance and Societal Importance of the Research Achievements

昆虫の体表ワックスは個体認識あるいは種を認識するためのセミオケミカルとして機能している。フタホシコオロギを用いた本研究により、体表ワックスが共食い行動においても使用されることが初めて示された。本研究で同定したCHC生合成酵素などは、今後、脂質合成酵素など、共食い研究や化学生態学的研究だけでなく、代謝の生化学的なアプローチを行う研究に貢献することが期待される。
また、昆虫の生態系や代謝に関する研究においてだけでなく、行動学や生理学的な「共食い行動」という本能行動を分子レベルで理解するために貢献すると考えられる。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (14 results)

All 2023 2022 2021

All Journal Article (6 results) (of which Peer Reviewed: 5 results,  Open Access: 4 results) Presentation (8 results)

  • [Journal Article] Best practices for comprehensive annotation of neuropeptides of Gryllus bimaculatus2023

    • Author(s)
      Takako Mochizuki、Mika Sakamoto、Yasuhiro Tanizawa、Hitomi Seike、Zhen Zhu、Yi Jun Zhou、Keisuke Fukumura、Shinji Nagata、Yasukazu Nakamura
    • Journal Title

      Insects

      Volume: 14(2) Issue: 2 Pages: 121-121

    • DOI

      10.3390/insects14020121

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] CCHamide-2 signaling regulates food intake and metabolism in Gryllus bimaculatus2022

    • Author(s)
      Zhu Z, Tsuchimoto M, Nagata S
    • Journal Title

      Insects

      Volume: 13 Issue: 4 Pages: 324-324

    • DOI

      10.3390/insects13040324

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Transcriptional levels of Corazonin, Elevenin, and PDF in the differently colored two-spotted cricket Gryllus bimaculatus2022

    • Author(s)
      Seike H, Nagata S
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 86 Issue: 1 Pages: 23-30

    • DOI

      10.1093/bbb/zbab183

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fatty Acid-derived Chemicals in Insects; Biosynthetic Pathways and Functions2022

    • Author(s)
      永田晋治、清家瞳
    • Journal Title

      Oleoscience

      Volume: 22 Issue: 4 Pages: 165-171

    • DOI

      10.5650/oleoscience.22.165

    • ISSN
      1345-8949, 2187-3461
    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] 雑食性昆虫における栄養分選好性摂食行動の制御メカニズム2022

    • Author(s)
      永田晋治、清家瞳
    • Journal Title

      アグリバイオ

      Volume: 6 Pages: 79-82

    • Related Report
      2021 Research-status Report
  • [Journal Article] Identification and characterization of 24-dehydrocholesterol reductase (DHCR24) in the two-spotted cricket, Gryllus bimaculatus.2021

    • Author(s)
      Mack YS Isa, Dehari M, Morooka N, Nagata S
    • Journal Title

      Insects

      Volume: 12 Issue: 9 Pages: 782-782

    • DOI

      10.3390/insects12090782

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] フタホシコオロギにおいて肉食性餌として認識される化合物の探索2023

    • Author(s)
      清家瞳、永田晋治
    • Organizer
      日本農芸化学会2023年度大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] フタホシコオロギにおける免疫応答に関与するphenloxidaseの同定2023

    • Author(s)
      宮原 佐季、清家 瞳、永田 晋治
    • Organizer
      日本農芸化学会2023年度大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 昆虫種一斉分析によるステロール組成比と食性の関連性2023

    • Author(s)
      南波 竜政、永里 鴻多、永田 晋治
    • Organizer
      日本農芸化学会2023年度大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] フタホシコオロギGryllusbimaculatusの神経ペプチドと受容体の包括的アノテーションの事例紹介2022

    • Author(s)
      望月孝子、坂本美佳、清家瞳、祝貞、Zhu Yijun、永田晋治、中村保一
    • Organizer
      日本分子生物学会2022年度大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] フタホシコオロギの体色変化を制御するペプチドホルモンの探索2022

    • Author(s)
      清家瞳、永田晋治
    • Organizer
      日本分子生物学会2022年度大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Allatotropin regulates reproduction behaviors by controlling the physiology of the male accessory gland of the twospotted crickets, Gryllus bimaculatus2022

    • Author(s)
      祝貞、永田晋治
    • Organizer
      日本分子生物学会2022年度大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Nutrient selective behavior under endocrine control in the cricket2022

    • Author(s)
      Shinji Nagata, Zhou Yi-Jun, Sayuki Oka, Yusuke Tsukamoto, Hitomi Seike, Keisuke Fukumura
    • Organizer
      International congress of entomology 2022
    • Related Report
      2022 Annual Research Report
  • [Presentation] NPF signaling regulates feeding preference in the two-spotted cricket, Gryllus bimaculatus2022

    • Author(s)
      Hitomi Seike, Shinji Nagata
    • Organizer
      International congress of entomology 2022
    • Related Report
      2022 Annual Research Report

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Published: 2020-08-03   Modified: 2024-01-30  

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