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Construction of a white-rot fungi-bacteria complex system that completes lignocellulose conversion by microbial reaction

Research Project

Project/Area Number 20K21341
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 40:Forestry and forest products science, applied aquatic science, and related fields
Research InstitutionUniversity of Miyazaki

Principal Investigator

Kamei Ichiro  宮崎大学, 農学部, 教授 (90526526)

Co-Investigator(Kenkyū-buntansha) 上村 直史  長岡技術科学大学, 工学研究科, 准教授 (50646528)
Project Period (FY) 2020-07-30 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2021: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2020: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords白色腐朽菌 / 細菌 / 木材腐朽 / 微生物間相互作用 / 複合微生物系 / リグニン / 共培養 / セルロース / ブタノール発酵 / 複合微生物
Outline of Research at the Start

本研究は、木材等のリグノセルロースバイオマスを構成する3つの主要高分子成分であるリグニン、セルロースおよびヘミセルロースを、複合微生物構築を基礎とした新規一貫バイオプロセスにより完全分解・変換する技術を開発するために、主要高分子成分を分解する異種微生物を共存させる技術を開発し、木材腐朽をミミックしたリグノセルロース変換技術開発に挑戦する。

Outline of Final Research Achievements

When nine species of wood-rotting fungi and the bacterial strain SYK-6 were grown against each other on PDA medium, bacterium was found to act in an inhibitory manner on the mycelial growth of many wood-rotting fungi. On the other hand, the inhibitory effect of the bacterium for wood-rotting fungi on the weight loss of wood dust was almost negligible, while the wood sawdust degradation by Trametes versicolor, Sarcomyxa serotine, Ceriporiopsis subvermispora, and Daedalea dickinsii was tended to be enhanced by the bacterial addition. Furthermore, lignin degradation was enhanced in co-culture with Sarcomyxa serotine and Ceriporiopsis subvermispora with bacterium.

Academic Significance and Societal Importance of the Research Achievements

本研究成果は、特定の細菌株と木材腐朽菌を共培養することで、木材の腐朽を効率よく進めるコンソーシアを構築可能であることを示すものである。生物的に大変困難である木材の分解を複合微生物系で促進可能であることを見出したことは、木材腐朽菌単独で研究が進む木材分解において、学術的意義がある。今後の課題として、細菌株がどのようにして木材腐朽菌のリグニン分解を促進しているのかについて、リグニン分解断片の分析定量を行う必要があるが、これらリグノセルロースの分解における複合微生物の意義を明らかにする第一歩として本研究成果は役立つと考えられる。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (20 results)

All 2023 2022 2021 2020 Other

All Int'l Joint Research (2 results) Journal Article (8 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 8 results,  Open Access: 2 results) Presentation (6 results) (of which Invited: 1 results) Book (4 results)

  • [Int'l Joint Research] Ha Long University(ベトナム)

    • Related Report
      2021 Research-status Report
  • [Int'l Joint Research] Institut Teknologi Sepuluh Nopember(インドネシア)

    • Related Report
      2021 Research-status Report
  • [Journal Article] Butanol production from alkaline pretreated bamboo using co-culture of wood-rotting fungus Phlebia sp. MG-60-P2 and bacterium Clostridium saccharoperbutylacetonicum2023

    • Author(s)
      Chu Luong Tri、Le Duy Khuong、Kamei Ichiro
    • Journal Title

      Biomass Conversion and Biorefinery

      Volume: inpress

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Selective Homologous Expression of Recombinant Manganese Peroxidase Isozyme of Salt-Tolerant White-Rot Fungus Phlebia sp. MG-60, and Its Salt-Tolerance and Thermostability2022

    • Author(s)
      Kamei, I., Tomitaka, N., Taichi, M., Yamasaki, Y.
    • Journal Title

      Journal of Microbiology and Biotechnology

      Volume: 32 Issue: 2 Pages: 248-255

    • DOI

      10.4014/jmb.2108.08042

    • Related Report
      2022 Annual Research Report 2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Biodegradation of DDT by Co-cultures of Pleurotus eryngii and Pseudomonas aeruginosa2021

    • Author(s)
      Maulianawati Diana、Purnomo Adi Setyo、Kamei Ichiro
    • Journal Title

      HAYATI Journal of Biosciences

      Volume: 28 Issue: 3 Pages: 240-240

    • DOI

      10.4308/hjb.28.3.240

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Interaction and Effects of Bacteria Addition on Dichlorodiphenyltrichloroethane Biodegradation by Daedalea dickinsii2021

    • Author(s)
      Rizqi Hamdan Dwi、Purnomo Adi Setyo、Kamei Ichiro
    • Journal Title

      Current Microbiology

      Volume: 78 Issue: 2 Pages: 668-678

    • DOI

      10.1007/s00284-020-02305-8

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] 微生物反応のみでバイオマス変換を完結するー生物触媒としての白色腐朽菌による脱リグニン同時糖化発酵ー2021

    • Author(s)
      亀井一郎
    • Journal Title

      化学と生物

      Volume: 59 Pages: 137-143

    • NAID

      130008165569

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Synergistic interaction of a consortium of the brown-rot fungus Fomitopsis pinicola and the bacterium Ralstonia pickettii for DDT biodegradation2020

    • Author(s)
      Purnomo Adi Setyo、Sariwati Atmira、Kamei Ichiro
    • Journal Title

      Heliyon

      Volume: 6 Issue: 6 Pages: e04027-e04027

    • DOI

      10.1016/j.heliyon.2020.e04027

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Butanol production from cellulosic material by anaerobic co-culture of white-rot fungus Phlebia and bacterium Clostridium in consolidated bioprocessing2020

    • Author(s)
      Tri Chu Luong、Kamei Ichiro
    • Journal Title

      Bioresource Technology

      Volume: 305 Pages: 123065-123065

    • DOI

      10.1016/j.biortech.2020.123065

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Conservation of Xylose Fermentability in Phlebia Species and Direct Fermentation of Xylan by Selected Fungi2020

    • Author(s)
      Kamei Ichiro、Uchida Kana、Ardianti Virginia
    • Journal Title

      Applied Biochemistry and Biotechnology

      Volume: 192 Issue: 3 Pages: 895-909

    • DOI

      10.1007/s12010-020-03375-x

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Presentation] マイタケ栽培原木における共存細菌の探索と解析2021

    • Author(s)
      陳 富嘉、亀井 一郎
    • Organizer
      第66回リグニン討論会
    • Related Report
      2021 Research-status Report
  • [Presentation] 木材の糖化と発酵を可能にするきのこの力2021

    • Author(s)
      亀井一郎
    • Organizer
      第46回 日本応用糖質科学会九州支部講演会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] 白色腐朽菌 Phlebia brevispora と共存細菌 Enterobacter sp.との接触が 誘導する菌糸伸長促進効果と細菌の移動2021

    • Author(s)
      下川 洋太,亀井 一郎
    • Organizer
      第71回日本木材学会大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 木材蒸気式乾燥廃液からのセクイリン C およびアガサレジノールの回収2021

    • Author(s)
      長田 健吾,菅本 和寛,亀井 一郎
    • Organizer
      第71回日本木材学会大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 耐塩性白色腐朽菌 Phlebia sp. MG-60 株の塩ストレス応答時における MAP キナーゼ HOG1 の役割2021

    • Author(s)
      元田 多一,亀井 一郎
    • Organizer
      第71回日本木材学会大会
    • Related Report
      2020 Research-status Report
  • [Presentation] Diversity of bacterial communities on maitake cultivated log2021

    • Author(s)
      FU-CHIA CHEN, Ichiro Kamei
    • Organizer
      第71回日本木材学会大会
    • Related Report
      2020 Research-status Report
  • [Book] Fungal Biotechnology Prospects and Avenues2022

    • Author(s)
      Chu Luong Tri、Le Duy Khuong、Kamei Ichiro
    • Total Pages
      16
    • Publisher
      CRC Press
    • ISBN
      9781003248316
    • Related Report
      2022 Annual Research Report
  • [Book] Fungal Biotechnology Prospects and Avenues2022

    • Author(s)
      Edited By Sunil K. Deshmukh, Kandikere R. Sridhar, Susanna M. Badalyan
    • Total Pages
      450
    • Publisher
      CRC Press
    • ISBN
      9781032163857
    • Related Report
      2021 Research-status Report
  • [Book] きのこの生物活性と応用展開2021

    • Author(s)
      河岸洋和
    • Total Pages
      360
    • Publisher
      シーエムシー出版
    • ISBN
      9784781316222
    • Related Report
      2021 Research-status Report
  • [Book] Fungi in Fuel Biotechnology2020

    • Author(s)
      Ichiro Kamei
    • Total Pages
      25
    • Publisher
      Springer
    • ISBN
      9783030444884
    • Related Report
      2020 Research-status Report

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Published: 2020-08-03   Modified: 2024-01-30  

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