Application of a viral vector to develop anti-poultry red mite vaccines targeting antigens expressed on the plasma membrane of midgut cells

• Project/Area Number: 20K21357
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 42:Veterinary medical science, animal science, and related fields
• Research Institution: Hokkaido University
• Principal Investigator: Murata Shiro 北海道大学, 獣医学研究院, 准教授 (10579163)
• Project Period (FY): 2020-07-30 – 2023-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2022: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2021: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2020: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ワクモ / 外部寄生虫 / マレック病 / マレック病ウイルス / 組換えウイルス / 組換えワクチン / 弱毒生ワクチン / ウイルスベクター / 中腸 / 細胞膜タンパク質

Outline of Research at the Start

ワクモ(鳥類の外部寄生虫)による吸血被害は、薬剤耐性ワクモの出現により、世界中の養鶏場で深刻化している。新規防除法としてワクチンが注目されている。本ワクチンは、ワクモが吸血時に抗体を取り込むことで効果をもたらすため、最初に抗体に暴露される腸管の細胞膜上に発現する分子は最も影響を受けやすいと考えられる。しかしその多くは複数回膜貫通型など、組換えタンパク質の作製には困難が予想される。本研究では、組換えウイルス感染細胞に細胞膜タンパク質としてワクチン抗原を発現させることで、有効な免疫応答を誘導すること目的としており、利用が困難であった抗原の応用や、高い効果を持つワクチン開発への発展が期待される。

Outline of Final Research Achievements

Poultry red mites (PRMs) are haematophagous ectoparasites which cause serious economic losses to the poultry industry, and vaccine approaches are focused on as a novel strategy to control PRMs. This vaccine acts when antibodies in the host blood are taken up into PRMs during blood sucking, and therefore, the molecules associated with the plasma membrane of cells in the intestinal tracts are considered to be susceptible. In this study, we successfully identified effective antigens, consistent with above criteria. However, plasma membrane-associated proteins often have complicated structures. To overcome the problems on the protein expression, we investigated the potential of the viral vector using attenuated strains of Marek’s disease virus (MDV). We examined several regions in the virus genome for the insertion of the vaccine antigen gene. However, the protein expression levels were low, and therefore, further manipulation is required for the application of MDV as a viral vector.

Academic Significance and Societal Importance of the Research Achievements

本ワクチンによって産生される抗体はワクモの腸管に最初に曝露されるため、腸管の細胞膜上に発現する分子は有用なワクチン抗原になると考えられる。本研究では、上記の条件に合致し、有用なワクチン抗原の同定に成功したため、より効果の高いワクチン開発に向けて有用な知見を提供できたと考えられる。一方、ワクチン抗原の発現やワクチン使用時の利便性に関する問題解決のため、ウイルスベクターの応用についても検討したが、本研究ではワクチン抗原の発現量の増加に関する改良を必要とする結果であった。本研究の結果を基盤として、さらなる改変を加えることで、効果および利便性の高いワクモ防除用ワクチンの開発への貢献が期待される。

Research Products

• [Journal Article] Investigation of peripheral blood responses in chickens infested with Dermanyssus gallinae. (2023)
  • Author(s): Fujisawa S, Murata S, Sato T, Oishi E, Taneno A, Konnai S, Ohashi K.
  • Journal Title: Parasitology International Volume: 95 Pages: 102754-102754
  • DOI: 10.1016/j.parint.2023.102754
  • Peer Reviewed
• [Journal Article] Analysis of gene expression in poultry red mite, <i>Dermanyssus gallinae</i>, by RNAscope <i>in situ</i> hybridization (2023)
  • Author(s): Seo H, Murata S, Ichii O, Namba T, Win SY, Sato T, Oishi E, Taneno A, Maekawa N, Okagawa T, Konnai S, Ohashi K.
  • Journal Title: Journal of Veterinary Medical Science Volume: 85 Issue: 5 Pages: 532-535
  • DOI: 10.1292/jvms.22-0544
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] Potential of ferritin 2 as an antigen for the development of a universal vaccine for avian mites, poultry red mites, tropical fowl mites, and northern fowl mites. (2023)
  • Author(s): Win, S. Y., Murata, S., Fujisawa, S., Seo, H., Sato, J., Motai, Y., Sato, T., Oishi, E., Taneno, A., Htun, L. L., Bawm, S., Okagawa, T., Maekawa, N., Konnai, S., Ohashi, K.
  • Journal Title: Frontiers in Veterinary Science Volume: 印刷中 Pages: 1182930-1182930
  • DOI: 10.3389/fvets.2023.1182930
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Enhancement of Vaccine-Induced T-Cell Responses by PD-L1 Blockade in Calves (2023)
  • Author(s): Okagawa T, Konnai S, Nakamura H, Ganbaatar O, Sajiki Y, Watari K, Noda H, Honma M, Kato Y, Suzuki Y, Maekawa N, Murata S, Ohashi K.
  • Journal Title: Vaccines (Basel) Volume: 11(3) Issue: 3 Pages: 559-559
  • DOI: 10.3390/vaccines11030559
  • Peer Reviewed / Open Access
• [Journal Article] Suppressive modulation of host immune responses by Dermanyssus gallinae infestation. (2023)
  • Author(s): Fujisawa, S., Murata, S., Isezaki, M., Win, S-Y., Sato, T., Oishi, E., Taneno, A., Maekawa, N., Okagawa, T., Konnai, S., Ohashi, K.
  • Journal Title: Poultry Science Volume: 102 Issue: 4 Pages: 102352-102352
  • DOI: 10.1016/j.psj.2023.102532
  • Peer Reviewed / Open Access
• [Journal Article] Characterization of SpsQ from Staphylococcus pseudintermedius as an affinity chromatography ligand for canine therapeutic antibodies (2023)
  • Author(s): Takeuchi H, Nakajima C, Konnai S, Maekawa N, Okagawa T, Usui M, Tamura Y, Suzuki Y, Murata S, Ohashi K.
  • Journal Title: PLOS ONE Volume: 18 Issue: 1 Pages: e0281171-e0281171
  • DOI: 10.1371/journal.pone.0281171
  • Peer Reviewed / Open Access
• [Journal Article] Molecular characterization of feline immune checkpoint molecules and establishment of PD-L1 immunohistochemistry for feline tumors (2023)
  • Author(s): Maekawa N, Konnai S, Asano Y, Otsuka T, Aoki E, Takeuchi H, Kato Y, Kaneko MK, Yamada S, Kagawa Y, Nishimura M, Takagi S, Deguchi T, Ohta H, Nakagawa T, Suzuki Y, Okagawa T, Murata S, Ohashi K.
  • Journal Title: PLOS ONE Volume: 18 Issue: 1 Pages: e0281143-e0281143
  • DOI: 10.1371/journal.pone.0281143
  • Peer Reviewed / Open Access
• [Journal Article] Combined Immune Checkpoint Blockade Enhances Antiviral Immunity against Bovine Leukemia Virus. (2023)
  • Author(s): Nakamura H, Konnai S, Okagawa T, Maekawa N, Sajiki Y, Watari K, Kamitani K, Saito M, Kato Y, Suzuki Y, Murata S, Ohashi K.
  • Journal Title: J Virol. Volume: 97(1) Issue: 1
  • DOI: 10.1128/jvi.01430-22
  • Peer Reviewed / Open Access
• [Journal Article] Diagnosis and Early Prediction of Lymphoma Using High-Throughput Clonality Analysis of Bovine Leukemia Virus-Infected Cells (2022)
  • Author(s): Okagawa T, Shimakura H, Konnai S, Saito M, Matsudaira T, Nao N, Yamada S, Murakami K, Maekawa N, Murata S, Ohashi K.
  • Journal Title: Microbiol Spectr. Volume: 10(6) Issue: 6
  • DOI: 10.1128/spectrum.02595-22
  • Peer Reviewed / Open Access
• [Journal Article] Prostaglandin E2-Induced Immune Suppression via Cytotoxic T-Lymphocyte Antigen 4 in Paratuberculosis (2022)
  • Author(s): Sajiki Y, Konnai S, Watari K, Okagawa T, Tanaka A, Kawaji S, Nagata R, Maekawa N, Suzuki Y, Kato Y, Murata S, Mori Y, Ohashi K.
  • Journal Title: Infection and Immunity Volume: 90 Issue: 10
  • DOI: 10.1128/iai.00210-22
  • Peer Reviewed / Open Access
• [Journal Article] Exploration of serum biomarkers in dogs with malignant melanoma receiving anti-PD-L1 therapy and potential of COX-2 inhibition for combination therapy (2022)
  • Author(s): Maekawa N, Konnai S, Asano Y, Sajiki Y, Deguchi T, Okagawa T, Watari K, Takeuchi H, Takagi S, Hosoya K, Kim S, Ohta H, Kato Y, Suzuki Y, Murata S, Ohashi K.
  • Journal Title: Scientific Reports Volume: 12 Issue: 1 Pages: 9265-9265
  • DOI: 10.1038/s41598-022-13484-8
  • Peer Reviewed / Open Access
• [Journal Article] Suppressive effects of Ixodes persulcatus sialostatin L2 against Borrelia miyamotoi-stimulated immunity (2022)
  • Author(s): Sajiki Y, Konnai S, Okagawa T, Maekawa N, Isezaki M, Yamada S, Ito T, Sato K, Kawabata H, Logullo C, da Silva Vaz Jr I, Murata S, Ohashi K.
  • Journal Title: Ticks and Tick-borne Diseases Volume: 13 Issue: 4 Pages: 101963-101963
  • DOI: 10.1016/j.ttbdis.2022.101963
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] In vitro evaluation of a cysteine protease from poultry red mites, Demanyssus gallinae, as a vaccine antigen for chickens (2022)
  • Author(s): Ariizumi, T., Murata, S., Fujisawa, S., Isezaki, M., Sato, T., Oishi, E., Taneno, A., Ichii, O., Maekawa, N., Okagawa, T., Konnai, S., Ohashi, K.
  • Journal Title: Poultry Science Volume: 101 Issue: 3 Pages: 101638-101638
  • DOI: 10.1016/j.psj.2021.101638
  • Peer Reviewed / Open Access
• [Journal Article] Effect of Insertion and Deletion in the Meq Protein Encoded by Highly Oncogenic Marek’s Disease Virus on Transactivation Activity and Virulence (2022)
  • Author(s): Sato, J., Murata, S., Yang, Z., Kaufer, B.B., Fujisawa, S., Seo, H., Maekawa, N., Okagawa, T., Konnai, S., Osterrieder, N., Parcells, M.S., Ohashi, K.
  • Journal Title: Viruses Volume: 14 Issue: 2 Pages: 382-382
  • DOI: 10.3390/v14020382
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Estradiol-induced immune suppression via prostaglandin E2 during parturition in bovine leukemia virus-infected cattle (2022)
  • Author(s): Sajiki Yamato、Konnai Satoru、Okagawa Tomohiro、Maekawa Naoya、Goto Shinya、Kohara Junko、Nitanai Atsushi、Takahashi Hirofumi、Kubota Kentaro、Takeda Hiroshi、Murata Shiro、Ohashi Kazuhiko
  • Journal Title: PLOS ONE Volume: 17 Issue: 3 Pages: 1-13
  • DOI: 10.1371/journal.pone.0263660
  • Peer Reviewed / Open Access
• [Journal Article] Enhancement of interleukin-2 production by bovine peripheral blood mononuclear cells treated with the combination of anti-programmed death-ligand 1 and cytotoxic T lymphocyte antigen 4 chimeric monoclonal antibodies (2022)
  • Author(s): WATARI Kei、KONNAI Satoru、OKAGAWA Tomohiro、MAEKAWA Naoya、SAJIKI Yamato、KATO Yukinari、SUZUKI Yasuhiko、MURATA Shiro、OHASHI Kazuhiko
  • Journal Title: Journal of Veterinary Medical Science Volume: 84 Issue: 1 Pages: 6-15
  • DOI: 10.1292/jvms.21-0552
  • NAID: 130008139952
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] Characterization of a copper transporter 1 from Dermanyssus gallinae as a vaccine antigen (2021)
  • Author(s): Fujisawa Sotaro、Murata Shiro、Isezaki Masayoshi、Ariizumi Takuma、Sato Takumi、Oishi Eiji、Taneno Akira、Maekawa Naoya、Okagawa Tomohiro、Ichii Osamu、Konnai Satoru、Ohashi Kazuhiko
  • Journal Title: Parasitology Volume: 149 Issue: 1 Pages: 105-115
  • DOI: 10.1017/s0031182021001608
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Characterization of a novel cysteine protease inhibitor from poultry red mites: potential vaccine for chickens (2021)
  • Author(s): Fujisawa S, Murata S, Isezaki M, Ariizumi T, Sato T, Oishi E, Taneno A, Maekawa N, Okagawa T, Ichii O, Konnai S, Ohashi K.
  • Journal Title: Vaccines (Basel) Volume: 9 Issue: 12 Pages: 1472-1472
  • DOI: 10.3390/vaccines9121472
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Research Note: Characterization of S-Meq containing the deletion in Meq protein's transactivation domain in a Marek's disease virus strain in Japan (2021)
  • Author(s): Murata, S., Yamamoto, E., Sakashita, N., Maekawa, N., Okagawa, T., Konnai, S., Ohashi, K.
  • Journal Title: Poultry Science Volume: 100 Issue: 11 Pages: 101461-101461
  • DOI: 10.1016/j.psj.2021.101461
  • Peer Reviewed / Open Access
• [Journal Article] In vitro characterization of adipocyte plasma membrane-associated protein from poultry red mites, Dermanyssus gallinae, as a vaccine antigen for chickens (2021)
  • Author(s): Fujisawa Sotaro、Murata Shiro、Takehara Masaki、Aoyama Julia、Morita Ayu、Isezaki Masayoshi、Win Shwe Yee、Ariizumi Takuma、Sato Takumi、Oishi Eiji、Taneno Akira、Maekawa Naoya、Okagawa Tomohiro、Ichii Osamu、Konnai Satoru、Ohashi Kazuhiko
  • Journal Title: Vaccine Volume: 39 Issue: 41 Pages: 6057-6066
  • DOI: 10.1016/j.vaccine.2021.08.104
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Programmed death‐ligand 1 expression in swine chronic infections and enhancement of interleukin‐2 production via programmed death‐1/programmed death‐ligand 1 blockade (2021)
  • Author(s): Ganbaatar, O., Konnai, S., Okagawa, T., Nojima, Y., Maekawa, N., Ichikawa, Y., Kobayashi, A., Shibahara, T., Yanagawa, Y., Higuchi, H., Kato, Y., Suzuki, Y., Murata, S., Ohashi, K.
  • Journal Title: Immunity, Inflammation and Disease Volume: 9 Issue: 4 Pages: 1573-1583
  • DOI: 10.1002/iid3.510
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Canine Transforming Growth Factor-β Receptor 2-Ig: A Potential Candidate Biologic for Melanoma Treatment That Reverses Transforming Growth Factor-β1 Immunosuppression (2021)
  • Author(s): Takeuchi H, Konnai S, Maekawa N, Takagi S, Ohta H, Sasaki N, Kim S, Okagawa T, Suzuki Y, Murata S, Ohashi K.
  • Journal Title: Front Vet Sci. Volume: 14;8 Pages: 656715-656715
  • DOI: 10.3389/fvets.2021.656715
  • Peer Reviewed / Open Access
• [Journal Article] Prostaglandin-related immune suppression in cattle (2021)
  • Author(s): Sajiki Y.、Konnai S.、Ikenaka Y.、Okagawa T.、Maekawa N.、Logullo C.、da Silva Vaz I.、Murata S.、Ohashi K.
  • Journal Title: Veterinary Immunology and Immunopathology Volume: 236 Pages: 110238-110238
  • DOI: 10.1016/j.vetimm.2021.110238
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Cross-species reactivity of antibodies against Ixodes persulcatus ferritin 2 to Rhipicephalus microplus (2021)
  • Author(s): Xavier, M.A., Konnai, S., Parizi, L.F., Githaka, N.W., Isezaki, M., Goto, S., Fujisawa, S., Yamada, S., Okagawa, T., Maekawa, N., Logullo, C., da Silva Vaz, Jr. I., Murata, S., Ohashi, K.
  • Journal Title: Japanese Journal of Veterinary Research Volume: 69 Issue: 1 Pages: 57-65
  • DOI: 10.14943/jjvr.69.1.57
  • NAID: 120006994840
  • ISSN: 0047-1917
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Intrauterine infection with Mycobacterium avium subsp. paratuberculosis in pregnant cattle diagnosed with Johne's disease (2021)
  • Author(s): Taniguchi, Y., Konnai, S., Sakakibara, S., Yamamoto, A., Okagawa, T., Maekawa, N., Murata, S., Ohashi, K.
  • Journal Title: Japanese Journal of Veterinary Research Volume: 69 Issue: 1 Pages: 51-55
  • DOI: 10.14943/jjvr.69.1.51
  • NAID: 120006994839
  • ISSN: 0047-1917
  • Peer Reviewed
• [Journal Article] Selection of reference genes for quantitative PCR analysis in poultry red mite (<i>Dermanyssus gallinae</i>) (2021)
  • Author(s): Ariizuim, T., Murata, S., Fujisawa, S., Isezaki, M., Maekawa, N., Okagawa, T., Sato, T., Oishi, E., Taneno, A., Konnai, S., Ohashi, K.
  • Journal Title: Journal of Veterinary Medical Science Volume: 83 Issue: 4 Pages: 558-565
  • DOI: 10.1292/jvms.20-0677
  • NAID: 130008023262
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] Characterisation of a cysteine protease from poultry red mites and its potential use as a vaccine for chickens (2021)
  • Author(s): Murata, S., Taniguchi, A., Isezaki, M., Fujisawa, S., Sakai, E., Taneno, A., Ichii, O., Ito, T., Maekawa, N., Okagawa, T., Konnai, S., Ohashi, K.
  • Journal Title: Parasite Volume: 28 Pages: 9-9
  • DOI: 10.1051/parasite/2021005
  • Peer Reviewed / Open Access
• [Journal Article] PD-L1 immunohistochemistry for canine cancers and clinical benefit of anti-PD-L1 antibody in dogs with pulmonary metastatic oral malignant melanoma (2021)
  • Author(s): Maekawa, N., Konnai, S., Nishimura, M., Kagawa, Y., Takagi, S., Hosoya, K., Ohta, H., Kim, S., Okagawa, T., Izumi, Y., Deguchi, T., Kato, Y., Yamamoto, S., Yamamoto, K., Toda, M., Nakajima, C., Suzuki, Y., Murata, S., Ohashi, K.
  • Journal Title: npj Precision Oncology Volume: 5 Issue: 1 Pages: 10-10
  • DOI: 10.1038/s41698-021-00147-6
  • Peer Reviewed / Open Access
• [Journal Article] The enhancement of Th1 immune response by anti-PD-L1 antibody in cattle infected with <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> (2021)
  • Author(s): Sajiki, Y., Konnai, S., Nagata, R., Kawaji, S., Nakamura, H., Fujisawa, S., Okagawa, T., Mawkawa, N., Kato, Y., Suzuki, Y., Murata, S., Mori, Y., Ohashi, K.
  • Journal Title: Journal of Veterinary Medical Science Volume: 83 Issue: 2 Pages: 162-166
  • DOI: 10.1292/jvms.20-0590
  • NAID: 130007990713
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] Direct evidence of the preventive effect of milk replacer?based probiotic feeding in calves against severe diarrhea (2021)
  • Author(s): Kayasaki, F., Okagawa, T., Konnai, S., Kohara, J., Sajiki, Y., Watari, K., Ganbaatar, O., Goto, S., Nakamura, H., Shimakura, H., Minato, E., Kobayashi, A., Kubota, M., Terasaki, N., Takeda, A., Noda, H., Honma, M., Maekawa, N., Murata, S., Ohashi, K.
  • Journal Title: Veterinary Microbiology Volume: 254 Pages: 108976-108976
  • DOI: 10.1016/j.vetmic.2020.108976
  • Peer Reviewed / Open Access
• [Journal Article] Tick saliva-induced programmed death-1 and PD-ligand 1 and its related host immunosuppression (2021)
  • Author(s): Sajiki, Y., Konnai, S., Ikenaka, Y., Gulay, K. C. M., Kobayashi, A. Parizi, L. F., Joo, B. C., Watari, K., Fujisawa, S., Okagawa, T., Maekawa, N., Logullo, C.,da Silva Vaz, I.,Murata, S., Ohashi, K.
  • Journal Title: Scientific Reports Volume: 11 Issue: 1 Pages: 1063-1063
  • DOI: 10.1038/s41598-020-80251-y
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A TLR7 agonist activates bovine Th1 response and exerts antiviral activity against bovine leukemia virus (2021)
  • Author(s): Sajiki, Y., Konnai, S., Okagawa, T., Maekawa, N., Nakamura, H., Kato, Y., Suzuki, Y., Murata, S., Ohashi, K.
  • Journal Title: Developmental & Comparative Immunology Volume: 114 Pages: 103847-103847
  • DOI: 10.1016/j.dci.2020.103847
  • Peer Reviewed / Open Access
• [Journal Article] Molecular detection and genetic characterization of infectious laryngotracheitis virus in poultry in Myanmar (2020)
  • Author(s): Yang, Z., Murata, S., Fujisawa, S., Takehara, M., Katakura, Ken., Hmoon, M. M., Win, S. Y., Bawm, S., Konnai, S., Ohashi, K.
  • Journal Title: BMC Veterinary Research Volume: 16 Issue: 1 Pages: 453-453
  • DOI: 10.1186/s12917-020-02666-z
  • Peer Reviewed / Open Access
• [Journal Article] Distinct polymorphisms in a single herpesvirus gene are capable of enhancing virulence and mediating vaccinal resistance (2020)
  • Author(s): Conradie, A. M., Bertzbach, L. D., Trimpert, J., Patria, J. N., Murata, S., Parcells M. S.,Kaufer, B. B.
  • Journal Title: PLOS Pathogens Volume: 16 Issue: 12 Pages: e1009104-e1009104
  • DOI: 10.1371/journal.ppat.1009104
  • Peer Reviewed / Open Access
• [Journal Article] Genetic characterization of a Marek’s disease virus strain isolated in Japan (2020)
  • Author(s): Murata, S., Machida, Y., Isezaki, M., Maekawa, N., Okagawa, T., Konnai, S., Ohashi, K.
  • Journal Title: Virology Journal Volume: 17 Issue: 1 Pages: 186-186
  • DOI: 10.1186/s12985-020-01456-1
  • Peer Reviewed / Open Access
• [Journal Article] Detection and molecular identification of Leucocytozoon and Plasmodium species from village chickens in different areas of Myanmar (2020)
  • Author(s): Win, S. Y., Chel, H. M., Hmoon, M. M., Htun, L. L., Bawm, S., Win, M. M., Murata, S., Nonaka, N., Nakao, R., Katakura, K.
  • Journal Title: Acta Tropica Volume: 212 Pages: 105719-105719
  • DOI: 10.1016/j.actatropica.2020.105719
  • Peer Reviewed
• [Journal Article] Transcriptome dynamics of blood-fed and starved poultry red mites, Dermanyssus gallinae (2020)
  • Author(s): Fujisawa, S., Murata, S., Isezaki, M., Oishi, E., Taneno, A., Maekawa, N., Okagawa, T., Konnai, S., Ohashi, K.
  • Journal Title: Parasitology International Volume: 78 Pages: 102156-102156
  • DOI: 10.1016/j.parint.2020.102156
  • NAID: 120007160301
  • Peer Reviewed
• [Journal Article] Enhanced Immunotherapeutic Efficacy of Anti?PD-L1 Antibody in Combination with an EP4 Antagonist (2020)
  • Author(s): Sajiki Yamato、Konnai Satoru、Cai Zimeng、Takada Kensuke、Okagawa Tomohiro、Maekawa Naoya、Fujisawa Sotaro、Kato Yukinari、Suzuki Yasuhiko、Murata Shiro、Ohashi Kazuhiko
  • Journal Title: ImmunoHorizons Volume: 4 Issue: 12 Pages: 837-850
  • DOI: 10.4049/immunohorizons.2000089
  • Peer Reviewed / Open Access
• [Journal Article] The Suppression of Th1 Response by Inducing TGF-β1 From Regulatory T Cells in Bovine Mycoplasmosis (2020)
  • Author(s): Sajiki Yamato、Konnai Satoru、Goto Shinya、Okagawa Tomohiro、Ohira Kosuke、Shimakura Honami、Maekawa Naoya、Gondaira Satoshi、Higuchi Hidetoshi、Tajima Motoshi、Hirano Yuki、Kohara Junko、Murata Shiro、Ohashi Kazuhiko
  • Journal Title: Frontiers in Veterinary Science Volume: 7 Pages: 2564-2569
  • DOI: 10.3389/fvets.2020.609443
  • NAID: 120007193553
  • Peer Reviewed / Open Access
• [Journal Article] PD-L1 expression in equine malignant melanoma and functional effects of PD-L1 blockade (2020)
  • Author(s): Ganbaatar, O., Konnai, S. Okagawa, T., Nojima, Y., Maekawa, N., Minato, E., Kobayashi, A., Ando, R., Sasaki, N., Miyakoshi, D., Ichii, O., Kato, Y., Suzuki, Y., Murata, S., Ohashi, K.
  • Journal Title: PLOS ONE Volume: 15 Issue: 11 Pages: e0234218-e0234218
  • DOI: 10.1371/journal.pone.0234218
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification and functional analysis of ferritin 2 from the Taiga tick Ixodes persulcatus Schulze (2020)
  • Author(s): Githaka, N. W., Konnai, S., Isezaki, M., Goto, S., Xavier, M. A., Fujisawa, S., Yamada, S., Okagawa, T., Maekawa, N., Logullo, C., da Silva Vaz, I., Murata, S., Ohashi, K. 4.巻 11
  • Journal Title: Ticks and Tick-borne Diseases Volume: 11 Issue: 6 Pages: 101547-101547
  • DOI: 10.1016/j.ttbdis.2020.101547
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Expression Analysis of Canine CMTM6 and CMTM4 as Potential Regulators of the PD-L1 Protein in Canine Cancers (2020)
  • Author(s): Takeuchi, H., Konnai, S., Maekawa, N., Minato, E., Ichikawa, Y., Kobayashi, A., Okagawa, T., Murata, S., Ohashi, K.
  • Journal Title: Frontiers in Veterinary Science Volume: 7 Pages: 330-330
  • DOI: 10.3389/fvets.2020.00330
  • Peer Reviewed / Open Access
• [Journal Article] Upregulation of PD-L1 Expression by Prostaglandin E2 and the Enhancement of IFN-γ by Anti-PD-L1 Antibody Combined With a COX-2 Inhibitor in Mycoplasma bovis Infection (2020)
  • Author(s): Goto Shinya、Konnai Satoru、Hirano Yuki、Kohara Junko、Okagawa Tomohiro、Maekawa Naoya、Sajiki Yamato、Watari Kei、Minato Erina、Kobayashi Atsuhi、et al.
  • Journal Title: Frontiers in Veterinary Science Volume: 7 Pages: 12-12
  • DOI: 10.3389/fvets.2020.00012
  • Peer Reviewed / Open Access
• [Journal Article] Immunosuppressive Effects of Sialostatins L and L2 Isolated from the Taiga Tick Ixodes persulcatus Schulze (2020)
  • Author(s): Sajiki Y, Konnai S, Ochi A, Okagawa T, Githaka N, Isezaki M, Yamada S, Ito T, Ando S, Kawabata H, Da Silva Vaz Jr I, Logullo C, Maekawa N, Murata S, Ohashi K.
  • Journal Title: Ticks Tick born Dis. Volume: 11(2) Issue: 2 Pages: 101332-101332
  • DOI: 10.1016/j.ttbdis.2019.101332
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Characterization of cysteine proteases from avian mites to develop a vaccine with broad efficacy (2022)
  • Author(s): Win Shwe Yee, 村田 史郎, 藤澤 宗太郎, 瀬尾 光里, 佐藤 純平, 佐藤 匠, 大石, 英司, 種子野 章, 岡川 朋弘, 前川 直也, 今内 覚, 大橋 和彦
  • Organizer: 第165回日本獣医学会学術集会
• [Presentation] ワクモ由来の2種類のアスパラギン酸プロテアーゼの性状解析 (2022)
  • Author(s): 瀬尾 光里, 村田 史郎, Shwe Yee Win, 藤澤 宗太郎, 北條 巧, 伊勢崎 政美, 佐藤 匠, 大石 英司, 種子野 章, 市居 修, 前川 直也, 岡川 朋弘,今内 覚, 大橋 和彦
  • Organizer: 第165回日本獣医学会学術集会
• [Presentation] ワクモ（Demanyssus gallinae）由来シスタチン様分子の抗ワクモワクチン抗原としての評価 (2022)
  • Author(s): 藤澤 宗太郎, 村田 史郎, 伊勢崎 政美, 有泉 拓馬, 佐藤 匠, 大石 英司, 種子野 章, 前川 直也, 岡川 朋弘, 市居 修, 今内 覚, 大橋 和彦
  • Organizer: 第165回日本獣医学会学術集会
• [Presentation] 乳酸菌 Lactobacillus plantarum HOKKAIDO株による免疫抑制機序の解明 (2022)
  • Author(s): 池端 麻里, 今内 覚, 岡川 朋弘, 阿部 健太郎, 本間 満, 北村 亨, 前川 直也, 村田 史郎, 大橋 和彦
  • Organizer: 第165回日本獣医学会学術集会
• [Presentation] ネコ腫瘍組織におけるprogrammed death ligand 1 (PD-L1) の発現解析 (2022)
  • Author(s): 青木 絵理, 今内 覚, 前川 直也, 岡川 朋弘, 竹内 寛人, 佐藤 純平, 浅野 裕美恵, 大塚 拓海, 賀川 由美子, 加藤 幸成, 高木 哲, 鈴木 定彦, 村田 史郎, 大橋 和彦
  • Organizer: 第165回日本獣医学会学術集会
• [Presentation] Effects of an Insertion and a Deletion in the Marek’s Disease Virus Oncogene Meq on Transactivation Activity and Virulence (2022)
  • Author(s): Jumpei Sato, Shiro Murata, Zhiyuan Yang, Benedikt B. Kaufer, Sotaro Fujisawa, Hikari Seo, Naoya Maekawa, Tomohiro Okagawa, Satoru Konnai, Nikolaus Osterrieder, Mark S. Parcells, Kazuhiko Ohashi
  • Organizer: 46th International Herpesvirus Workshop
  • Int'l Joint Research
• [Presentation] ワクモ（Dermanyssus gallinae）の吸血に対する宿主免疫応答の解析 (2021)
  • Author(s): 藤沢 宗太郎, 村田 史郎, 伊勢崎 政美, 佐藤 匠, 大石 英司, 種子野 章, 前川 直也, 岡川 朋弘, 今内 覚, 大橋 和彦
  • Organizer: 第164回日本獣医学会学術集会
• [Presentation] 鼻疽に対する診断用抗原の探索および血清診断法の確立 (2021)
  • Author(s): 飯沼 由希帆, 岡川 朋弘, 市川 世識, 陸 拾七, 前川 直也, 村田 史郎, 今内 覚, 木村 享史, 大橋 和彦
  • Organizer: 第164回日本獣医学会学術集会
• [Presentation] Expression and functional analysis of swine PD-1/PD-L1 pathway (2021)
  • Author(s): Otgontuya Ganbaatar, 今内 覚, 岡川 朋弘, 野島 裕太郎, 前川 直也, 市川 世識, 小林 篤史, 芝原 友幸, 柳川 洋二郎, 鈴木 定彦, 村田 史郎, 大橋 和彦
  • Organizer: 第164回日本獣医学会学術集会
• [Presentation] ウシCTLA-4ならびにPD-L1を標的とした抗体併用法による免疫増強効果の検討 (2021)
  • Author(s): 渡 慧, 今内 覚, 岡川 朋弘, 前川 直也, 村田 史郎, 鈴木 定彦, 大橋 和彦
  • Organizer: 第164回日本獣医学会学術集会
• [Presentation] 牛伝染性リンパ腫発症母牛における子宮内感染の診断 (2021)
  • Author(s): 嶋倉 穂南, 今内 覚, 岡川 朋弘, 中村 隼人, 神谷 可菜, 齋藤 麻矢, 前川 直也, 村田 史郎, 大橋 和彦
  • Organizer: 第164回日本獣医学会学術集会
• [Presentation] マレック病ウイルスMeqタンパク質における挿入および欠損配列の病原性への影響 (2021)
  • Author(s): 佐藤 純平, 村田 史郎, 藤澤 宗太郎, 瀬尾 光里, 前川 直也, 岡川 朋弘, 今内 覚, 大橋 和彦
  • Organizer: 第164回日本獣医学会学術集会
• [Presentation] 牛白血病ウイルス感染症における免疫抑制受容体TIM-3の発現解析および機能解析 (2020)
  • Author(s): 中村 隼人, 今内 覚, 岡川 朋弘, 佐治木 大和, 渡 慧, 神谷 可菜, 齋藤 麻矢, 前川 直也, 村田 史郎, 大橋 和彦
  • Organizer: 第163回日本獣医学会学術集会
• [Presentation] ネコCytotoxic T-lymphocyte antigen-4 (CTLA-4) を標的とした新規免疫抑制製剤の開発における基礎的検討 (2020)
  • Author(s): 大塚 拓海, 今内 覚, 前川 直也, 渡 慧, 岡川 朋弘, 村田 史郎, 大橋 和彦
  • Organizer: 第163回日本獣医学会学術集会
• [Presentation] ワクモにおける定量的PCR法確立のための内在性コントロール遺伝子の探索 (2020)
  • Author(s): 有泉 拓馬, 村田 史郎, 藤澤 宗太郎, 伊勢崎 政美, 前川 直也, 岡川 朋弘, 種子野 章, 大石 英司, 今内 覚, 大橋 和彦
  • Organizer: 第163回日本獣医学会学術集会
• [Remarks] 北海道大学大学院獣医学研究院 感染症学教室ホームページ
  • URL: https://lab-inf.vetmed.hokudai.ac.jp
• [Patent(Industrial Property Rights)] 抗ワクモワクチン組成物及びその使用 (2021)
  • Inventor(s): 村田 史郎, 大橋 和彦, 藤澤 宗太郎, 他6名
  • Industrial Property Rights Holder: 国立大学法人北海道大学, ワクチノーバ株式会社
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2021-080691
  • Filing Date: 2021