Development of an information/hypothesis-driven proteogenomics strategy
Project/Area Number |
20K21386
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Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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Allocation Type | Multi-year Fund |
Review Section |
Medium-sized Section 43:Biology at molecular to cellular levels, and related fields
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Research Institution | Niigata University |
Principal Investigator |
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Project Period (FY) |
2020-07-30 – 2023-03-31
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Project Status |
Completed (Fiscal Year 2022)
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Budget Amount *help |
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2021: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2020: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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Keywords | プロテオミクス / プロテオゲノミクス / 質量分析 / プロテオーム / タンパク質 / 定量プロテオミクス |
Outline of Research at the Start |
生命システムの本質である多様性を理解する上で、核酸オミクス情報とプロテオームの間の関数の解明が極めて重要な課題である。本研究は、申請者らが構築した大規模精密定量プロテオーム解析技術iMPAQT法を発展させ、核酸情報とプロテオーム情報の間に存在するブラックボックスの解明に挑むことを目的とする。任意のペプチド配列に対する同位体標識ペプチドを大規模に取得する技術とそれを支援するデータベースを構築することで、その実現を試みる。
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Outline of Final Research Achievements |
In this study, we developed a technology to link nucleic acid information and proteome information through "information/hypothesis-driven proteogenomics," a strategy that is opposite to existing proteogenomics research. We created an isotope-labeled internal standard production system using E. coli, and developed QuantiCode, an ultra-multiplex quantitative tag system for large-scale absolute quantification, and Quantimer, which functions as an internal standard. Furthermore, we developed the SLiM method, a new ultra-sensitive mass spectrometry technique that seamlessly links two mass spectrometry techniques called DIA and PRM, and conducted a proof-of-concept experiment for proteogenomics using this method.
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Academic Significance and Societal Importance of the Research Achievements |
本研究によって、任意のペプチド配列の存在を確定的に検証することが可能となり、プロテオームに存在する多数の未確認タンパク質を同定あるいは定量が可能となった。これらの新規タンパク質の発見や機能解析は、ゲノム機能の注釈やタンパク質のミスセンス変異やスプライシング異常などがタンパク質発現への影響を知る手段となるため、学術的な意義はとても大きい。また、真のプロテオームの把握は、基礎生物学的な意義だけでなく、疾患研究や創薬における基盤的情報となるため、将来的に人類の健康等に貢献できると期待される。
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Candesartan prevents arteriopathy progression in cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy model2021
Author(s)
Kato T, Manabe RI, Igarashi H, Kametani F, Hirokawa S, Sekine Y, Fujita N, Saito S, Kawashima Y, Hatano Y, Ando S, Nozaki H, Sugai A, Uemura M, Fukunaga M, Sato T, Koyama A, Saito R, Sugie A, Toyoshima Y, Kawata H, Murayama S, Matsumoto M, Kakita A, Hasegawa M, Ihara M, Kanazawa M, Nishizawa M, Tsuji S, Onodera O.
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Journal Title
Journal of Clinical Investigation
Volume: 131
Issue: 22
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Senolysis by glutaminolysis inhibition ameliorates various age-associated disorders2021
Author(s)
Johmura Yoshikazu、Yamanaka Takehiro、Omori Satotaka、Wang Teh-Wei、Sugiura Yuki、Matsumoto Masaki、Suzuki Narumi、Kumamoto Soichiro、Yamaguchi Kiyoshi、Hatakeyama Seira、Takami Tomoyo、Yamaguchi Rui、Shimizu Eigo、Ikeda Kazutaka、Okahashi Nobuyuki、Mikawa Ryuta、Suematsu Makoto、Arita Makoto、Sugimoto Masataka et al
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Journal Title
Science
Volume: 371
Issue: 6526
Pages: 265-270
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] The Autism-Related Protein SETD5 Controls Neural Cell Proliferation through Epigenetic Regulation of rDNA Expression.2020
Author(s)
Nakagawa T, Hattori S, Nobuta R, Kimura R, Nakagawa M, Matsumoto M, Nagasawa Y, Funayama R, Miyakawa T, Inada T, Osumi N, Nakayama KI, Nakayama K.
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Journal Title
iScience
Volume: 23
Issue: 4
Pages: 101030-101030
DOI
Related Report
Peer Reviewed / Open Access
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