Establishment of a simple and rapid method for assessing amyloid polymorphism for pathophysiological diagnosis

• Project/Area Number: 20K21396
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 43:Biology at molecular to cellular levels, and related fields
• Research Institution: Kobe University
• Principal Investigator: Chatani Eri 神戸大学, 理学研究科, 准教授 (00432493)
• Project Period (FY): 2020-07-30 – 2023-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2022: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2021: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2020: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 蛋白質 / ミスフォールディング / アミロイド / 多形

Outline of Research at the Start

一種類のタンパク質から様々なアミロイド線維構造が形成するという「アミロイド多形」は、アミロイドーシスにおいて異なる病態や感染性をもたらすと考えられる。しかし、これを簡便かつ鋭敏に識別できる手法は十分に確立されていない。そこで本研究では、アミロイド線維がヨウ素で染まる現象に着眼し、ヨウ素をプローブとしてアミロイド多形を評価する方法を構築する。さらにこれを利用し、アミロイド多形の毒性への関わりと感染時の構造伝播の様子を調べる。同時に、病態診断への応用も目指してアミロイド多形のイメージングにも挑戦する。

Outline of Final Research Achievements

This study has been conducted aiming to construct a simple and rapid method for identifying amyloid polymorphism. Through the analysis of insulin amyloid fibrils, we have demonstrated that iodine staining is useful for distinguishing amyloid polymorphs. Furthermore, as an application example of this method, we tracked the preservation and changes of the structure of amyloid fibrils during seeding reactions. We also tried iodine staining of multiple types of amyloid fibrils and confirmed the applicability of iodine staining to a wide range of proteins.

Academic Significance and Societal Importance of the Research Achievements

アミロイド多形は、異なる病態や感染性をもたらす構造的な要因であることが指摘されているものの、迅速かつ鋭敏に識別する方法は見出されていなかった。ヨウ素染色はアミロイド線維の構造多形を幅広く識別することが示されたことから、現状を打開し、アミロイド研究での活用だけでなく新たな病態診断法への展開が期待される。

Research Products

• [Int'l Joint Research] ザガジグ大学(エジプト)
• [Int'l Joint Research] 台湾国立陽明交通大学(その他の国・地域)
• [Int'l Joint Research] ザガジグ大学(エジプト)
• [Int'l Joint Research] ザガジグ大学(エジプト)
• [Journal Article] Two-stage optical trapping and assembling of protein at air/solution interface (2023)
  • Author(s): Yi Po-Wei、Chiu Wei-Hsiang、Toyouchi Shuichi*、Bresoli-Obach Roger*、Hofkens Johan、Chatani Eri、Hosokawa Yoichiroh*、Sugiyama Teruki*、Masuhara Hiroshi*
  • Journal Title: Applied Physics Express Volume: 16 Issue: 2 Pages: 025501-025501
  • DOI: 10.35848/1882-0786/acb3ab
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Tracking the Structural Development of Amyloid Precursors in the Insulin B Chain and the Inhibition Effect by Fibrinogen (2022)
  • Author(s): Naoki Yamamoto, Rintaro Inoue, Yoshiteru Makino, Hiroshi Sekiguchi, Naoya Shibayama, Akira Naito, Masaaki Sugiyama, and Eri Chatani
  • Journal Title: The Journal of Physical Chemistry B Volume: 126 Issue: 51 Pages: 10797-10812
  • DOI: 10.1021/acs.jpcb.2c05136
  • Peer Reviewed / Open Access
• [Journal Article] Pathway Dependence of the Formation and Development of Prefibrillar Aggregates in Insulin B Chain (2022)
  • Author(s): Yoshikawa Yuki、Yuzu Keisuke、Yamamoto Naoki、Morishima Ken、Inoue Rintaro、Sugiyama Masaaki、Iwasaki Tetsushi、So Masatomo、Goto Yuji、Tamura Atsuo、Chatani Eri
  • Journal Title: Molecules Volume: 27 Issue: 13 Pages: 3964-3964
  • DOI: 10.3390/molecules27133964
  • Peer Reviewed / Open Access
• [Journal Article] Cooperative Optical Trapping of Polystyrene Microparticle and Protein Forming a Submillimeter Linear Assembly of Microparticle (2021)
  • Author(s): Po-Wei Yi,Wei-Hsiang Chiu,Tetsuhiro Kudo,Teruki Sugiyama,Roger Bresoli-Obach,Johan Hofkens,Eri Chatani,Ryohei Yasukuni,Yoichiroh Hosokawa,Shuichi Toyouchi,Hiroshi Masuhara
  • Journal Title: J. Phys. Chem. C Volume: 125 Issue: 34 Pages: 18988-18999
  • DOI: 10.1021/acs.jpcc.1c05796
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Current Understanding of the Structure, Stability and Dynamic Properties of Amyloid Fibrils (2021)
  • Author(s): Chatani Eri、Yuzu Keisuke、Ohhashi Yumiko、Goto Yuji
  • Journal Title: International Journal of Molecular Sciences Volume: 22 Issue: 9 Pages: 4349-4349
  • DOI: 10.3390/ijms22094349
  • NAID: 120007038650
  • Peer Reviewed / Open Access
• [Journal Article] アミロイド線維前駆中間体の観測とそれを標的とした線維形成阻害―インスリン由来モデルペプチドとフィブリノーゲンを用いた検証― (2021)
  • Author(s): 山本 直樹,茶谷 絵理
  • Journal Title: 生物物理 Volume: 61 Pages: 236-239
  • NAID: 130008068985
  • Peer Reviewed / Open Access
• [Journal Article] アミロイド線維の伝播に見られる構造の保存と変化 (2021)
  • Author(s): 柚佳祐,茶谷絵理
  • Journal Title: C & I Commun Volume: 46 Pages: 1921-1921
• [Journal Article] Mechanism of Nucleation in the Formation of Amyloid Fibrils (2021)
  • Author(s): 茶谷 絵理
  • Journal Title: MEMBRANE Volume: 46 Issue: 1 Pages: 19-24
  • DOI: 10.5360/membrane.46.19
  • NAID: 130007977587
  • ISSN: 0385-1036, 1884-6440
  • Peer Reviewed
• [Journal Article] Functional Assembly of Caenorhabditis elegans Cytochrome b-2 (Cecytb-2) into Phospholipid Bilayer Nanodisc with Enhanced Iron Reductase Activity. (2021)
  • Author(s): Hamed A Abosharaf,Yuki Sakamoto,Aliaa M Radwan,Keisuke Yuzu,Mika Fujimura,Thoria Diab,Tarek M Mohamed,Eri Chatani,Tetsunari Kimura,Motonari Tsubaki
  • Journal Title: Biomolecules Volume: 11 Issue: 1 Pages: 96-96
  • DOI: 10.3390/biom11010096
  • NAID: 120007162571
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Breakdown of supersaturation barrier links protein folding to amyloid formation. (2021)
  • Author(s): Masahiro Noji,Tatsushi Samejima,Keiichi Yamaguchi,Masatomo So,Keisuke Yuzu,Eri Chatani,Yoko Akazawa-Ogawa,Yoshihisa Hagihara,Yasushi Kawata,Kensuke Ikenaka,Hideki Mochizuki,Jozsef Kardos,Daniel E Otzen,Vittorio Bellotti,Johannes Buchner,Yuji Goto
  • Journal Title: Communications biology Volume: 4 Issue: 1 Pages: 120-120
  • DOI: 10.1038/s42003-020-01641-6
  • NAID: 120007028968
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Theoretical modeling for electronic structure of polyiodide species included in α-cyclodextrin (2020)
  • Author(s): Masaki Okuda,Takato Hiramatsu,Mitsuru Yasuda,Mika Ishigaki,Yukihiro Ozaki,Michitoshi Hayashi,Keisuke Tominaga,Eri Chatani
  • Journal Title: J. Phys. Chem. B Volume: 124 Issue: 20 Pages: 4089-4096
  • DOI: 10.1021/acs.jpcb.0c01749
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Exploration of insulin amyloid polymorphism using Raman spectroscopy and imaging (2020)
  • Author(s): Mika Ishigaki,Kana Morimoto,Eri Chatani,Yukihiro Ozaki
  • Journal Title: Biophys. J. Volume: 118 Issue: 12 Pages: 2997-3007
  • DOI: 10.1016/j.bpj.2020.04.031
  • Peer Reviewed
• [Journal Article] Iodine staining as a useful probe for distinguishing insulin amyloid polymorphs. (2020)
  • Author(s): Takato Hiramatsu,Naoki Yamamoto,Seongmin Ha,Yuki Masuda,Mitsuru Yasuda,Mika Ishigaki,Keisuke Yuzu,Yukihiro Ozaki,Eri Chatani
  • Journal Title: Sci. Rep. Volume: 10 Issue: 1 Pages: 16741-16741
  • DOI: 10.1038/s41598-020-73460-y
  • NAID: 120006897864
  • Peer Reviewed / Open Access
• [Journal Article] タンパク質に対する圧力研究の新展開-基本熱力学から,分子構造に基づく理解,アミロイド線維まで (2020)
  • Author(s): 櫻井一正,茶谷絵理,後藤祐児
  • Journal Title: 化学 Volume: 75 Pages: 39-44
  • NAID: 40022384891
• [Journal Article] Multistep Changes in Amyloid Structure Induced by Cross-Seeding on a Rugged Energy Landscape. (2020)
  • Author(s): Keisuke Yuzu,Naoki Yamamoto,Masahiro Noji,Masatomo So,Yuji Goto,Tetsushi Iwasaki,Motonari Tsubaki,Eri Chatani
  • Journal Title: Biophys. J. Volume: 120 Issue: 2 Pages: 284-295
  • DOI: 10.1016/j.bpj.2020.12.005
  • Peer Reviewed / Open Access
• [Presentation] Two-stage optical trapping mechanism of protein at its air/solution interface (2023)
  • Author(s): Po-Wei Yi, Wei-Hsiang Chiu, Shuichi Toyouchi, Roger Bresol-Obach, Johan Hofkens, Eri Chatani, Yoichiroh Hosokawa, Teruki Sugiyama, and Hiroshi Masuhara
  • Organizer: 第70回応用物理学会春季学術講演会
  • Invited
• [Presentation] アミロイド前凝集体を標的とした分子シャペロンαB-クリスタリンの線維形成阻害様式 (2023)
  • Author(s): 茶谷絵理、國尾祐貴、柚佳祐、山本直樹、守島健、奥田綾、井上倫太郎、杉山正明、Junna Hayashi、John A. Carver
  • Organizer: 第14回 タンパク質の異常凝集とその防御・修復機構に関する研究会
  • Invited
• [Presentation] アミロイド核形成段階におけるオリゴマーおよびプロトフィブリルの形成機構 (2023)
  • Author(s): 柚佳祐、今村比呂志、野崎拓郎、藤井悠生、守島健、奥田綾、井上倫太郎、杉山正明、茶谷絵理
  • Organizer: 第14回 タンパク質の異常凝集とその防御・修復機構に関する研究会
  • Invited
• [Presentation] アミロイド線維の核形成メカニズムの解明 (2023)
  • Author(s): 茶谷絵理
  • Organizer: 第14回ナノ・マイクロ計測化学セミナー
  • Invited
• [Presentation] Study of oligomer and protofibril mediated amyloid nucleation: Mechanistic insights from insulin and its B chain (2023)
  • Author(s): Eri Chatani
  • Organizer: JSPS Core to Core Program and Technical University of Munich Joint Meeting: Amyloid Symposium Munich 2023
  • Int'l Joint Research / Invited
• [Presentation] トランスサイレチン49-127断片のアミロイド線維化反応の特徴と変異効果 (2022)
  • Author(s): 中尾星哉、柚佳祐、松村美里、大橋祐美子、茶谷絵理
  • Organizer: 若手フロンティア研究会2022
• [Presentation] ドデシル硫酸ナトリウムによるα-Synucleinの初期凝集体の捕捉と観察 (2022)
  • Author(s): 北野さくら、柚佳祐、笹田航、木村哲就、茶谷絵理
  • Organizer: 若手フロンティア研究会2022
• [Presentation] Mechanisms of oligomer and protofibril formation suggested through studies of insulin and its B chain (2022)
  • Author(s): Eri Chatani
  • Organizer: JSPS Core-to-Core Program and University of Wollongong Joint meeting: Protein aggregation, proteostasis and crocodiles
  • Int'l Joint Research / Invited
• [Presentation] Local conformation of intrinsically disordered regions of Sup35 produces temperature sensitivity of liquid-liquid phase separation (2022)
  • Author(s): Yumiko Ohhashi, Suguru Nishinami, Kentaro Shiraki, and Eri Chatani
  • Organizer: Proteostasis & Disease Symposium 2022
  • Int'l Joint Research / Invited
• [Presentation] Amyloid fibril formation of transthyretin induced by fragmentation (2022)
  • Author(s): Keisuke Yuzu, Misato Matsumura, Naoki Yamamoto, Masatomo So, Keiichi Yamaguchi, Yuji Goto, and Eri Chatani
  • Organizer: Proteostasis & Disease Symposium 2022
  • Int'l Joint Research
• [Presentation] Formation of two types of tau oligomers depending on redox conditions (2022)
  • Author(s): Ayumi Masui, Keisuke Yuzu, Keiichi Yamaguchi, Yuji Goto, Yasushi Kawata, and Eri Chatani
  • Organizer: Proteostasis & Disease Symposium 2022
  • Int'l Joint Research
• [Presentation] Conformational transitions of amyloid fibrils during cross-seeding (2022)
  • Author(s): Eri Chatani, Keisuke Yuzu, Naoki Yamamoto, Masahiro Noji, Masatomo So, Yuji Goto, Tetsushi Iwasaki, and Motonari Tsubaki
  • Organizer: Proteostasis & Disease Symposium 2022
  • Int'l Joint Research
• [Presentation] The chaperone function of αB-crystallin through the formation of a complex with the amyloid precursor of the insulin B-chain (2022)
  • Author(s): Yuki Kokuo, Keisuke Yuzu, Naoki Yamamoto, Ken Morishima, Rintaro Inoue, Masaaki Sugiyama, Junna Hayashi, John A. Carver, and Eri Chatani
  • Organizer: Proteostasis & Disease Symposium 2022
  • Int'l Joint Research
• [Presentation] アミロイド線維の発生と伝播の分子機構 (2022)
  • Author(s): 茶谷絵理
  • Organizer: 奈良先端科学技術大学院大学CDG共催光ナノセミナー
  • Invited
• [Presentation] Liquid-liquid phase separation and amyloid formation of Sup35 from four different yeast species (2022)
  • Author(s): 大橋祐美子、西奈美卓、白木賢太郎、茶谷絵理
  • Organizer: 第60回日本生物物理学会年会
• [Presentation] トランスサイレチン断片のアミロイド線維形成 (2022)
  • Author(s): 柚佳祐、松村美里、山本直樹、宗正智、山口圭一、後藤祐児、茶谷絵理
  • Organizer: 第60回日本生物物理学会年会
• [Presentation] Cooperative optical trapping dynamics of two proteins at solution surface (2022)
  • Author(s): Wei-Hsiang Chiu, Po-Wei Yi, Shuichi Toyouchi, Yoichiroh Hosokawa, Roger Bresol-Obach, Johan Hofkens, Eri Chatani, and Hiroshi Masuhara
  • Organizer: 第82回応用物理学会秋季学術講演会
• [Presentation] Study on solvent conditions of prion protein droplet formation for amyloid nucleation analysis (2022)
  • Author(s): Taiki Ozawa, Suguru Nishinami, Shunsuke Tomita, Yumiko Ohhashi, Motohiro Kasuya, Eri Chatani, Yoko Maruyama, Kentaro Shiraki, Akihide Hibara, and Mao Fukuyama
  • Organizer: 令和4年度化学系学協会東北大会
• [Presentation] 近赤外分光法を用いた，タンパク質の２次構造変化に伴う水分子の水素結合ネットワークの変化についての分析 (2022)
  • Author(s): 石垣美歌、加藤慎基、茶谷絵理
  • Organizer: 日本分析化学会第69年会
• [Presentation] Fluorescence imaging and Raman spectral analyses of optically evolved assembling of lysozyme at air/solution interface (2022)
  • Author(s): Po-Wei Yi, Shuichi Toyouchi, Wei-Hsiang Chiu, Roger Bresol-Obach, Johan Hofkens, Eri Chatani, Ryohei Yasukuni, Yoichiroh Hosokawa, Teruki Sugiyama, and Hiroshi Masuhara
  • Organizer: 2022年光化学討論会
• [Presentation] アミロイド線維の形成－核生成と伝播－の機構解明 (2022)
  • Author(s): 茶谷 絵理
  • Organizer: 広島大学 第25回RcMcD融合研究セミナー
  • Invited
• [Presentation] トランスサイレチン49-127断片のアミロイド線維化反応における塩の影響 (2022)
  • Author(s): 中尾星哉、柚佳祐、松村美里、大橋祐美子、茶谷絵理
  • Organizer: 第22回日本蛋白質科学会年会
• [Presentation] アミロイド線維形成におけるαシヌクレインの初期凝集機構の解明 (2022)
  • Author(s): 北野さくら、柚佳祐、笹田航、木村哲就、茶谷絵理
  • Organizer: 第22回日本蛋白質科学会年会
• [Presentation] タウのアミロイド線維形成に対する酸化還元状態の影響 (2022)
  • Author(s): 益井歩未、柚佳祐、山口圭一、後藤祐児、河田康志、茶谷絵理
  • Organizer: 第22回日本蛋白質科学会年会
• [Presentation] 低分子熱ショックタンパク質であるαB-クリスタリンは，インスリンB鎖のアミロイド前駆体と複合体を形成し，成長を阻害する (2022)
  • Author(s): 國尾祐貴、柚佳祐、山本直樹、Junna Hayashi、John A. Carver、守島健、井上倫太郎、杉山正明、茶谷絵理
  • Organizer: 第22回日本蛋白質科学会年会
• [Presentation] 断片化したトランスサイレチンのアミロイド線維形成 (2022)
  • Author(s): 柚佳祐、松村美里、山本直樹、宗正智、山口圭一、茶谷絵理
  • Organizer: 第22回日本蛋白質科学会年会
• [Presentation] Sup35天然変性領域の局所構造が液-液相分離の環境応答性を決定する (2022)
  • Author(s): 大橋祐美子、西奈美卓、白木賢太郎、茶谷絵理
  • Organizer: 第22回日本蛋白質科学会年会
• [Presentation] 液液相分離相からのアミロイド形成速度のサイズ依存 (2022)
  • Author(s): 福山真央、西奈美卓、冨田峻介、大橋祐美子、粕谷素洋、茶谷絵理、丸山洋子、白木賢太郎、火原彰秀
  • Organizer: 化学とマイクロ・ナノシステム学会 第45回研究会
  • Invited
• [Presentation] マイクロサイズプリオンタンパク質集合液滴中からのアミロイド核生成速度解析 (2022)
  • Author(s): 福山真央、西奈美卓、冨田峻介、大橋祐美子、粕谷素洋、茶谷絵理、白木賢太郎、火原 彰秀
  • Organizer: 第82回分析化学討論会
• [Presentation] Investigating protein assembly in the nucleation of amyloid fibrils (2022)
  • Author(s): Eri Chatani
  • Organizer: Molecular Basis of Proteinopathies
  • Int'l Joint Research / Invited
• [Presentation] アミロイド線維形成の初期に伴うタンパク質の集合・動態 (2022)
  • Author(s): 茶谷絵理
  • Organizer: 松ヶ崎サイエンスフォーラム
  • Invited
• [Presentation] Protein Assembly Formation by Cooperative Optical Trapping with Polyethylene Glycol at Solution Surface (2022)
  • Author(s): Wei-Hsiang Chiu,Po-Wei. Yi,Eri Chatani,Roger Bresoli-Obach,Johan Hofkens,Shuichi Toyouchi,Hiroshi Masuhara
  • Organizer: 第69回応用物理学会春季学術講演会
• [Presentation] Determination of amyloid nucleation rate in Sup35 droplet (2021)
  • Author(s): Mao Fukuyama,Suguru Nishinami,Shunsuke Tomita,Motohiro Kasuya,Yumiko Ohhashi,Eri Chatani,Kentaro Shiraki,Akihide Hibara
  • Organizer: 第二回分子夾雑化学国際会議
  • Int'l Joint Research
• [Presentation] タウのオリゴマー形成に対する酸化還元状態の影響 (2021)
  • Author(s): 益井 歩未,柚 佳祐,山口 圭一,後藤 祐児,河田 康志,茶谷 絵理
  • Organizer: 若手フロンティア研究会 2021
• [Presentation] αB-クリスタリンのアミロイド線維形成阻害機構 (2021)
  • Author(s): 國尾 祐貴,柚 佳祐,Junna Hayashi,John A. Caver,茶谷 絵理
  • Organizer: 若手フロンティア研究会 2021
• [Presentation] インスリンの由来種に依存したアミロイド核形成に先立つ初期凝集メカニズム (2021)
  • Author(s): 柚 佳祐,今村 比呂志,野崎 拓郎,藤井 悠生,守島 健,奥田 綾,井上 倫太郎,杉山 正明,茶谷 絵理
  • Organizer: 若手フロンティア研究会 2021
• [Presentation] Mechanism of protein assembly for the formation of amyloid fibrils (2021)
  • Author(s): Eri Chatani
  • Organizer: CEFMS Lecture on Protein Dynamics
  • Int'l Joint Research / Invited
• [Presentation] アミロイド線維の出現に至るまでのタンパク質分子集合メカニズムの理解 (2021)
  • Author(s): 茶谷 絵理
  • Organizer: 第44回日本分子生物学会年会
  • Invited
• [Presentation] Inhibition of amyloid fibrillation by αB-crystallin by preventing the growth of prefibrillar intermediates: Formation of a stable complex (2021)
  • Author(s): Yuki Kokuo,Keisuke Yuzu,Junna Hayashi,John A. Carver,Eri Chatani
  • Organizer: 第59回日本生物物理学会年会
• [Presentation] Early aggregation kinetics leading to the amyloid nucleation of bovine and human insulin (2021)
  • Author(s): Keisuke Yuzu,Hiroshi Imamura,Takuro Nozaki,Yuki Fujii,Ken Morishima,Aya Okuda,Rintaro Inoue,Masaaki Sugiyama,Keisuke Tominaga,Eri Chatani
  • Organizer: 第59回日本生物物理学会年会
• [Presentation] Analysis of Heparin-Induced Tau Oligomer Formation by Dynamic Light Scattering (2021)
  • Author(s): Ayumi Masui,Keisuke Yuzu,Keiichi Yamaguchi,Yuji Goto,Yasushi Kawata,Eri Chatani
  • Organizer: 第59回日本生物物理学会年会
• [Presentation] 線維前駆中間体に作用するαB-クリスタリンのアミロイド線維形成阻害機構 (2021)
  • Author(s): 國尾 祐貴,柚 佳祐,John A. Carver,茶谷 絵理
  • Organizer: 第94回日本生化学大会
• [Presentation] アミロイド核形成に先立つインスリンの由来種に依存した初期凝集メカニズム (2021)
  • Author(s): 柚 佳祐,今村 比呂志,野崎 拓郎,藤井 悠生,守島 健,奥田 綾,井上 倫太郎,杉山 正明,富永 圭介,茶谷 絵理
  • Organizer: 第94回日本生化学大会
• [Presentation] 動的光散乱法によるタウのオリゴマー形成機構の解析 (2021)
  • Author(s): 益井 歩未,柚 佳祐,後藤 祐児,山口 圭一,河田 康志,茶谷 絵理
  • Organizer: 第94回日本生化学大会
• [Presentation] Optically evolving condensation of protein: A sub-millimeter 3-dimensional domain formation from solution surface (2021)
  • Author(s): Shuichi Toyouchi,Po-Wei Yi,Wei-Hsiang Chiu,Roger Bresoli-Obach,Johan Hofkens,Eri Chatani,Teruki Sugiyama,Ryohei Yasukuni,Yoichiroh Hosokawa,Hiroshi Masuhara
  • Organizer: 2021年光化学討論会
• [Presentation] Optically Evolved Condensation of Lysozyme Study by Raman Microspectroscopy (2021)
  • Author(s): Po-Wei Yi,Wei-Hsiang Chiu,Tetsuhiro Kudo,Roger Bresoli-Obach,Johan Hofkens,Eri Chatani,Ryohei Yasukuni,Yoichiroh Hosokawa,Teruki Sugiyama,Shuichi Toyouchi,Hiroshi Masuhara
  • Organizer: 第82回応用学会秋季学術講演会
• [Presentation] Optical force-induced three-dimensional protein assembly growing from solution (2021)
  • Author(s): Roger Bresoli-Obach,Po-Wei Y,Wei-Hsiang Chiu,Tetsuhiro Kudo,Johan Hofkens,Eri Chatani,Ryohei Yasukuni,Yoichiroh Hosokawa,Teruki Sugiyama,Shuichi Toyouchi,Hiroshi Masuhara
  • Organizer: 19th Congress of the European Society for Photobiology
  • Int'l Joint Research
• [Presentation] Optical force-induced three-dimensional protein assembly growing from solution surface (2021)
  • Author(s): Po-Wei Yi,Wei-Hisang Chiu,Tetsuhiro Kudo,Roger Bresoli-Obach,Johan Hofkens,Eri Chatani,Ryohei Yasukuni,Yoichiroh Hosokawa,Teruki Sugiyama,Shuichi Toyouchi,Hiroshi Masuhara
  • Organizer: International Conference on Photochemistry 2021
  • Int'l Joint Research
• [Presentation] Sup35 の液- 液相分離におけるプリオンドメインの役割 (2021)
  • Author(s): 大橋 祐美子,西奈美 卓,白木 賢太郎,茶谷 絵理
  • Organizer: 第21回日本蛋白質科学会年会
• [Presentation] インスリンアミロイド線維の種間クロスシーディングに見られる構造の変化 (2021)
  • Author(s): 柚 佳祐,山本 直樹,野地 真広,宗 正智,後藤 祐児,岩崎 哲史,鍔木 基成,茶谷 絵理
  • Organizer: 第21回日本蛋白質科学会年会
• [Presentation] Early aggregation kinetics preceding the nucleation in distinct amyloid formation pathways between bovine and human insulin (2021)
  • Author(s): 柚 佳祐,今村 比呂志,野崎 拓郎,守島 健,奥田 綾,井上 倫太郎,杉山 正明,茶谷 絵理
  • Organizer: 第21回日本蛋白質科学会年会
• [Presentation] チオフラビンT 蛍光寿命アッセイによるインスリンアミロイド線維構造の識別 (2021)
  • Author(s): 小田 明典,犬伏 知生,柚 佳祐,茶谷 絵理,吉川 悦次,里園 浩
  • Organizer: 第21回日本蛋白質科学会年会
• [Presentation] Single Sub-Millimeter Linear Assembly of Polystyrene Microparticles (2021)
  • Author(s): Po-Wei. Yi,Wei-Hsiang Chiu,Tetsuhiro Kudo,Roger Bresoli-Obach,Johan Hofkens,Eri Chatani,Ryohei Yasukuni,Yoichiroh Hosokawa,Teruki Sugiyama,Shuichi Toyouchi,Hiroshi Masuhara
  • Organizer: 第68回応用物理学会春季学術講演会
• [Presentation] Structural difference and its seed-dependent propagation manner of human/bovine insulin amyloid fibrils as detected by iodine staining (2020)
  • Author(s): Keisuke Yuzu,Naoki Yamamoto,Masahiro Noji,Masatomo So,Yuji Goto,Tetsushi Iwasaki,Eri Chatani
  • Organizer: 第20回日本蛋白質科学会
• [Presentation] Amyloid Formation And Breakdown Of Supersaturation (2020)
  • Author(s): Masahiro Noji,Keiichi Yamaguchi,Eri Chatani,Yuji Goto
  • Organizer: 第20回日本蛋白質科学会年会
• [Presentation] Thioflavin T Fluorescence Lifetime Assay Of Homogeneous Insulin Amyloid Fibrils (2020)
  • Author(s): Akinori Oda,Tomoo Inubushi,Keisuke Yuzu,Eri Chatani,Etsuji Yoshikawa,Hiroshi Satozono
  • Organizer: 第20回日本蛋白質科学会年会
• [Presentation] Optical Trapping and Assembling of Protein at Solution Surface (2020)
  • Author(s): Po-Wei. Yi,Tetsuhiro Kudo,Eri Chatani,Ryohei Yasukuni,Yoichiroh Hosokawa,Teruki Sugiyama,Hiroshi Masuhara
  • Organizer: 第81回応用物理学会秋季学術講演会
• [Presentation] Cross-seeding of human and bovine insulin amyloid fibrils induces stepwise conformational transition via intermediate states (2020)
  • Author(s): Keisuke Yuzu,Naoki Yamamoto,Masahiro Noji,Masatomo So,Yuji Goto,Tetsushi Iwasaki,Motonari Tsubaki,Eri Chatani
  • Organizer: 第58回日本生物物理学会年会
• [Presentation] アミロイド線維形成とリン脂質二分子膜破壊との関係性の解明 (2020)
  • Author(s): Hiroki Takayama,Kaori Mageshi,Kenichi Morigaki,Eri Chatani
  • Organizer: 第58回日本生物物理学会年会
• [Presentation] Multi-step conformational changes via intermediate states induced by cross-seeding of human and bovine insulin amyloid fibrils (2020)
  • Author(s): Keisuke Yuzu,Naoki Yamamoto,Masahiro Noji,Masatomo So,Yuji Goto,Tetsushi Iwasaki,Motonari Tsubaki,Eri Chatani
  • Organizer: 第93回日本生化学会大会
• [Presentation] アミロイド線維形成過程におけるリン脂質二分子膜の膜破壊機構の解明 (2020)
  • Author(s): 高山 宙輝,曲師 香緒里,茶谷 絵理
  • Organizer: 第93回日本生化学会大会