Development of technology for high-resolution analysis of genome structure in the nucleus of tissues at the single-cell level.
| Project/Area Number |
20K21398
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 43:Biology at molecular to cellular levels, and related fields
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| Research Institution | Kyushu University |
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Principal Investigator |
Ohkawa Yasuyuki 九州大学, 生体防御医学研究所, 教授 (80448430)
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| Project Period (FY) |
2020-07-30 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2021: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2020: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | エピゲノム / クロマチン / マルチオミクス / 空間オミクス |
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| Outline of Research at the Start |
組織形成においては、様々な細胞が、増殖、分化していく極めて不均一な集団変化が起こり、その上個々の細胞においても少なくとも３０００遺伝子座以上が協調的あるいは排他的に秩序だった制御を受け、遺伝子発現あるいは抑制に必要な高次構造の変換が行われる。そこで本研究では特定の細胞分化・組織形成を理解するため、全ゲノム上の遺伝子を対象とし不均一な組織細胞を単一細胞レベルで時系列変化を解析する新技術開発を行う。
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| Outline of Final Research Achievements |
Living organisms are formed by the proliferation and differentiation of stem or progenitor cells, which in turn differentiate and proliferate into cells and tissues that carry out various functions. In this process, selective gene expression takes place. Selective gene expression is a dynamic event in which a specific gene locus is converted into an activating chromatin structure via regulatory regions (enhancers and promoters) of genes that are widely distributed in the genome region, resulting in the acquisition of a genomic structure that promotes transcriptional activity of the gene. We have developed a technology that resolves technical biases in the process of developing omics technology, while at the same time developing a technique for analyzing genomic higher-order structure at the single cell level, which is considered technically extremely difficult. Along with the development of the new technology, we acquired reference data by conducting pilot experiments.
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| Academic Significance and Societal Importance of the Research Achievements |
各細胞に固有のバーコードを付加する基本技術の構築を行い理論的におおよそ2000万細胞の固有ラベルを達成する技術を開発した。また、トランスクリプトーム解析をモデルとして単一細胞レベルでそれぞれの細胞内で非破壊的に固有のラベルを付加する技術を確立した。現在これらラベルの読み取り技術および同時解析技術の開発を進めている。これら技術は空間オミクスと呼ばれる組織の中の細胞の情報を取得する技術に活用できるとともに、ゲノム高次構造の解析にも有効であり、今後生物学上において重要な知見が得られることが期待できる。
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Report
(3 results)
Research Products
(49 results)
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[Journal Article] Uhrf1 governs the proliferation and differentiation of muscle satellite cells.2022
Author(s)
Sakai H, Sawada Y, Tokunaga N, Tanaka K, Nakagawa S, Sakakibara I, Ono Y, Fukada SI, Ohkawa Y, Kikugawa T, Saika T, Imai Y.
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Journal Title
iScience.
Volume: 25
Issue: 3
Pages: 103928-103928
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Uterus-specific transcriptional regulation underlies eggshell pigment production in Japanese quail.2022
Author(s)
Ishishita S, Kitahara S, Takahashi M, Iwasaki S, Tatsumoto S, Hara I, Kaneko Y, Kinoshita K, Yamaguchi K, Harada A, Ohmori Y, Ohkawa Y, Go Y, Shigenobu S, Matsuda Y, Suzuki T.
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Journal Title
Related Report
Peer Reviewed / Open Access
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[Journal Article] Relayed signaling between mesenchymal progenitors and muscle stem cells ensures adaptive stem cell response to increased mechanical load.2021
Author(s)
Kaneshige A, Kaji T, Zhang L, Saito H, Nakamura A, Kurosawa T, Ikemoto-Uezumi M, Tsujikawa K, Seno S, Hori M, Saito Y, Matozaki T, Maehara K, Ohkawa Y, Potente M, Watanabe S, Braun T, Uezumi A, Fukada SI.
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Journal Title
Cell Stem Cell.
Volume: 29
Issue: 2
Pages: 265-280
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Modeling population size independent tissue epigenomes by ChIL-seq with single thin sections.2021
Author(s)
Maehara K, Tomimatsu K, Harada A, Tanaka K, Sato S, Fukuoka M, Okada S, Handa T, Kurumizaka H, Saitoh N, Kimura H, Ohkawa Y.
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Journal Title
Mol Syst Biol.
Volume: 17
Related Report
Peer Reviewed / Open Access
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[Journal Article] Targeted inhibition of EPAS1-driven IL-31 production by a small-molecule compound.2021
Author(s)
Kamikaseda Y, Uruno T, Kunimura K, Harada A, Saiki K, Oisaki K, Sakata D, Nakahara T, Kido-Nakahara M, Kanai M, Nakamura S, Ohkawa Y, Furue M, Fukui Y.
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Journal Title
J Allergy Clin Immunol.
Volume: 148
Issue: 2
Pages: 633-638
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Hoxa10 mediates positional memory to govern stem cell function in adult skeletal muscle.2021
Author(s)
Yoshioka K, Nagahisa H, Miura F, Araki H, Kamei Y, Kitajima Y, Seko D, Nogami J, Tsuchiya Y, Okazaki N, Yonekura A, Ohba S, Sumita Y, Chiba K, Ito K, Asahina I, Ogawa Y, Ito T, Ohkawa Y, Ono Y.
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Journal Title
Sci Adv.
Volume: 7
Issue: 24
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Direct reprogramming of human umbilical vein- and peripheral blood-derived endothelial cells into hepatic progenitor.2020
Author(s)
Hiroki Inada, Miyako Udono , Kanae Matsuda-Ito, Kenichi Horisawa, Yasuyuki Ohkawa, Shizuka Miura, Takeshi Goya, Junpei Yamamoto, Masao Nagasaki, Kazuko Ueno, Daisuke Saitou, Mikita Suyama, Yoshihiko Maehara, Wataru Kumamaru, Yoshihiro Ogawa, Sayaka Sekiya, Atsushi Suzuki.
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Journal Title
cellsNat Commun.
Volume: 11
Issue: 1
Pages: 5292-5292
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] TKIs induce alternative spliced BCR-ABLIns35bp variant via inhibition of RNA polymerase Ⅱ on genomic BCR-ABL.2020
Author(s)
Yuda J, Odawara J, Minami M, Muta T, Kohno K, Tanimoto K, Eto T, Shima T, Kikushige Y, Kato K, Takenaka K, Iwasaki H, Minami Y, Ohkawa Y, Akashi K, Miyamoto T.
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Journal Title
Cancer Sci
Volume: 111
Issue: 7
Pages: 2361-2373
DOI
Related Report
Peer Reviewed / Open Access
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