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Production of novel drug derivery system with light-induced disruption of liposomes (LiDL)

Research Project

Project/Area Number 20K21482
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 47:Pharmaceutical sciences and related fields
Research InstitutionOkayama University

Principal Investigator

Sudo Yuki  岡山大学, 医歯薬学域, 教授 (10452202)

Co-Investigator(Kenkyū-buntansha) 山田 勇磨  北海道大学, 薬学研究院, 准教授 (60451431)
Project Period (FY) 2020-07-30 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2022: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2021: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2020: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsロドプシン / 光 / 生物物理 / pH / リポソーム / DDS / 薬物送達 / 物理系薬学
Outline of Research at the Start

【目的】物理化学(代表者)と薬剤学(分担者・協力者)の融合による光誘起崩壊リポソーム:Light-induced Disruption of Liposomes(LiDL)の開発と、それに基づく新奇薬物送達手法の確立。【背景】狙った時間と場所に薬物を届け・働かせることは、薬学における大きな『夢』である。【計画】光受容タンパク質・ロドプシンとpH感受性ポリマーおよび任意の化合物(薬物)を内封させたリポソームを開発することで、時空間制御性に優れた『光』により、狙った時間・場所で薬物を放出させる新奇手法を確立する。【意義】薬学における『夢』の一つを叶える手法となり、大きな波及効果をもたらす。

Outline of Final Research Achievements

The purpose of this research is to establish a novel drug delivery method with the light-induced disruption of liposomes (LiDL). Indeed, we created liposomes containing light-driven H+ pump rhodopsin and a pH-sensitive polymer, in which fluorescent molecules were enclosed. When the liposomes were exposed to light, the liposomes were acidified and the shape of the pH-sensitive polymer was changed, followed by liposome collapse and compound release. This method is expected to become a novel drug delivery system (DDS) that releases drugs by using light with high spatiotemporal resolution.

Academic Significance and Societal Importance of the Research Achievements

本研究により、異なる学術領域である物理化学(代表者)と薬剤学(分担者・協力者)の融合による光誘起崩壊リポソーム:Light-induced Disruption of Liposomes(LiDL)の開発と、それに基づく新奇薬物送達手法の確立が達成された。狙った時間と場所に薬物を届け・働かせることは、薬学における大きな『夢』であり、光を用いる本手法は、その『夢』の達成に向けての有用な手法として、薬学のみならず広く自然科学分野に大きな波及効果をもたらすものと期待される。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (45 results)

All 2023 2022 2021 2020 Other

All Int'l Joint Research (1 results) Journal Article (30 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 23 results,  Open Access: 20 results) Presentation (8 results) (of which Int'l Joint Research: 2 results,  Invited: 8 results) Remarks (1 results) Patent(Industrial Property Rights) (5 results) (of which Overseas: 2 results)

  • [Int'l Joint Research] The Pennsylvania State University/Montana State University(米国)

    • Related Report
      2022 Annual Research Report
  • [Journal Article] Identification of a Functionally Efficient and Thermally Stable Outward Sodium-Pumping Rhodopsin (<i>Be</i>NaR) from a Thermophilic Bacterium2023

    • Author(s)
      Kurihara Marie、Thiel Vera、Takahashi Hirona、Kojima Keiichi、Ward David M.、Bryant Donald A.、Sakai Makoto、Yoshizawa Susumu、Sudo Yuki
    • Journal Title

      Chemical and Pharmaceutical Bulletin

      Volume: 71 Issue: 2 Pages: 154-164

    • DOI

      10.1248/cpb.c22-00774

    • ISSN
      0009-2363, 1347-5223
    • Year and Date
      2023-02-01
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Detection of Membrane Potential-Dependent Rhodopsin Fluorescence Using Low-Intensity Light Emitting Diode for Long-Term Imaging2023

    • Author(s)
      Kawanishi Shiho、Kojima Keiichi、Shibukawa Atsushi、Sakamoto Masayuki、Sudo Yuki
    • Journal Title

      ACS Omega

      Volume: 8 Issue: 5 Pages: 4826-4834

    • DOI

      10.1021/acsomega.2c06980

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structure and mechanism of oxalate transporter OxlT in an oxalate-degrading bacterium in the gut microbiota2023

    • Author(s)
      Jaunet-Lahary Titouan、Shimamura Tatsuro、Hayashi Masahiro、Nomura Norimichi、Hirasawa Kouta、Shimizu Tetsuya、Yamashita Masao、Tsutsumi Naotaka、Suehiro Yuta、Kojima Keiichi、Sudo Yuki、Tamura Takashi、Iwanari Hiroko、Hamakubo Takao、Iwata So、Okazaki Kei-ichi、Hirai Teruhisa、Yamashita Atsuko
    • Journal Title

      Nature Communications

      Volume: 14 Issue: 1 Pages: 1730-1730

    • DOI

      10.1038/s41467-023-36883-5

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Convergent evolution of animal and microbial rhodopsins2023

    • Author(s)
      Kojima Keiichi、Sudo Yuki
    • Journal Title

      RSC Advances

      Volume: 13 Issue: 8 Pages: 5367-5381

    • DOI

      10.1039/d2ra07073a

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Editorial: Forewords to the special issue “Recent advances in retinal protein research”2023

    • Author(s)
      Sudo Yuki、Terakita Akihisa、Kandori Hideki
    • Journal Title

      Biophysics and Physicobiology

      Volume: 20 Issue: Supplemental Pages: n/a

    • DOI

      10.2142/biophysico.bppb-v20.s001

    • ISSN
      2189-4779
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Introduction of Session 1, “Photochemistry of retinal proteins”2023

    • Author(s)
      Sudo Yuki
    • Journal Title

      Biophysics and Physicobiology

      Volume: 20 Issue: Supplemental Pages: n/a

    • DOI

      10.2142/biophysico.bppb-v20.s021

    • ISSN
      2189-4779
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 膜蛋白質の可溶化(抽出)(3)2023

    • Author(s)
      小島慧一、須藤雄気
    • Journal Title

      蛋白質科学会アーカイブ

      Volume: 16

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mutations conferring SO42- pumping ability on the cyanobacterial anion pump rhodopsin and the resultant unique features of the mutant2022

    • Author(s)
      Yuhei Doi, Jo Watanabe, Rytota Nii, Takashi Tsukamoto, Makoto Demura, Yuki Sudo, Takashi Kikukawa
    • Journal Title

      Scientific Reports

      Volume: 12 Issue: 1 Pages: 16422-16422

    • DOI

      10.1038/s41598-022-20784-6

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 光をくすりに~光でがん細胞を自滅させる新しいがん治療法への期待2022

    • Author(s)
      須藤雄気、小島慧一
    • Journal Title

      MDB技術予測レポート

      Volume: na

    • Related Report
      2022 Annual Research Report
  • [Journal Article] 0.0000000003%の奇跡2022

    • Author(s)
      須藤雄気
    • Journal Title

      Seibutsu Butsuri

      Volume: 62 Issue: 1 Pages: 1-1

    • DOI

      10.2142/biophys.62.1

    • ISSN
      0582-4052, 1347-4219
    • Related Report
      2022 Annual Research Report
    • Open Access
  • [Journal Article] Activity of Regional Branch2022

    • Author(s)
      須藤 雄気
    • Journal Title

      Seibutsu Butsuri

      Volume: 62 Issue: 5 Pages: 312-313

    • DOI

      10.2142/biophys.62.312

    • ISSN
      0582-4052, 1347-4219
    • Related Report
      2022 Annual Research Report
    • Open Access
  • [Journal Article] Expression of microbial rhodopsins in Escherichia coli and their extraction and purification using styrene-maleic acid copolymers2022

    • Author(s)
      Kojima Keiichi、Sudo Yuki
    • Journal Title

      STAR Protocols

      Volume: 3 Issue: 1 Pages: 101046-101046

    • DOI

      10.1016/j.xpro.2021.101046

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Development of an Outward Proton Pumping Rhodopsin with a New Record in Thermostability by Means of Amino Acid Mutations2022

    • Author(s)
      Yasuda Satoshi、Akiyama Tomoki、Kojima Keiichi、Ueta Tetsuya、Hayashi Tomohiko、Ogasawara Satoshi、Nagatoishi Satoru、Tsumoto Kouhei、Kunishima Naoki、Sudo Yuki、Kinoshita Masahiro、Murata Takeshi
    • Journal Title

      The Journal of Physical Chemistry B

      Volume: 126 Issue: 5 Pages: 1004-1015

    • DOI

      10.1021/acs.jpcb.1c08684

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Phototriggered Apoptotic Cell Death (PTA) Using the Light-Driven Outward Proton Pump Rhodopsin Archaerhodopsin-32022

    • Author(s)
      Nakao Shin、Kojima Keiichi、Sudo Yuki
    • Journal Title

      Journal of the American Chemical Society

      Volume: 144 Issue: 9 Pages: 3771-3775

    • DOI

      10.1021/jacs.1c12608

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 光がくすりになる!?―ロドプシンによる生命機能の光操作2022

    • Author(s)
      須藤雄気、小島慧一
    • Journal Title

      月刊「化学」

      Volume: 77 Pages: 64-65

    • Related Report
      2021 Research-status Report
  • [Journal Article] 光+ロドプシン=くすり2022

    • Author(s)
      須藤雄気、小島慧一、川西志歩
    • Journal Title

      フォトニクスニュース

      Volume: 7 Pages: 153-158

    • Related Report
      2021 Research-status Report
  • [Journal Article] バイオマスを2倍にする新技術:ロドプシンを用いた緑藻クラミドモナスの生育制御2022

    • Author(s)
      小島慧一、長瀬友里恵、田村丞、須藤雄気
    • Journal Title

      クリーンエネルギー

      Volume: 31 Pages: 49-57

    • Related Report
      2021 Research-status Report
  • [Journal Article] Microbial Rhodopsins as Multi-functional Photoreactive Membrane Proteins for Optogenetics2021

    • Author(s)
      Nakao Shin、Kojima Keiichi、Sudo Yuki
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 44 Issue: 10 Pages: 1357-1363

    • DOI

      10.1248/bpb.b21-00544

    • NAID

      130008095840

    • ISSN
      0918-6158, 1347-5215
    • Year and Date
      2021-10-01
    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] An optogenetic assay method for electrogenic transporters using Escherichia coli co‐expressing light‐driven proton pump2021

    • Author(s)
      Hayashi Masahiro、Kojima Keiichi、Sudo Yuki、Yamashita Atsuko
    • Journal Title

      Protein Science

      Volume: 30 Issue: 10 Pages: 2161-2169

    • DOI

      10.1002/pro.4154

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Functional expression of the eukaryotic proton pump rhodopsin OmR2 in Escherichia coli and its photochemical characterization2021

    • Author(s)
      Kikuchi Masuzu、Kojima Keiichi、Nakao Shin、Yoshizawa Susumu、Kawanishi Shiho、Shibukawa Atsushi、Kikukawa Takashi、Sudo Yuki
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 14765-14765

    • DOI

      10.1038/s41598-021-94181-w

    • NAID

      120007147387

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structure of a retinal chromophore of dark-adapted middle rhodopsin as studied by solid-state nuclear magnetic resonance spectroscopy2021

    • Author(s)
      Kawamura Izuru、Seki Hayato、Tajima Seiya、Makino Yoshiteru、Shigeta Arisu、Okitsu Takashi、Wada Akimori、Naito Akira、Sudo Yuki
    • Journal Title

      Biophysics and Physicobiology

      Volume: advpub Issue: 0 Pages: 177-185

    • DOI

      10.2142/biophysico.bppb-v18.019

    • NAID

      130008072373

    • ISSN
      2189-4779
    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Proton transfer pathway in anion channelrhodopsin-12021

    • Author(s)
      Tsujimura Masaki、Kojima Keiichi、Kawanishi Shiho、Sudo Yuki、Ishikita Hiroshi
    • Journal Title

      eLife

      Volume: 10

    • DOI

      10.7554/elife.72264

    • NAID

      120007183236

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Exploring the Retinal Binding Cavity of Archaerhodopsin-3 by Replacing the Retinal Chromophore With a Dimethyl Phenylated Derivative2021

    • Author(s)
      Tsuneishi Taichi、Takahashi Masataka、Tsujimura Masaki、Kojima Keiichi、Ishikita Hiroshi、Takeuchi Yasuo、Sudo Yuki
    • Journal Title

      Frontiers in Molecular Biosciences

      Volume: 8 Pages: 794948-794948

    • DOI

      10.3389/fmolb.2021.794948

    • NAID

      120007183231

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Further thermo‐stabilization of thermophilic rhodopsin from Thermus thermophilus JL ‐18 through engineering in extramembrane regions2021

    • Author(s)
      Akiyama Tomoki、Kunishima Naoki、Nemoto Sayaka、Kazama Kazuki、Hirose Masako、Sudo Yuki、Matsuura Yoshinori、Naitow Hisashi、Murata Takeshi
    • Journal Title

      Proteins: Structure, Function, and Bioinformatics

      Volume: 89 Issue: 3 Pages: 301-310

    • DOI

      10.1002/prot.26015

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mechanism of absorption wavelength shifts in anion channelrhodopsin-1 mutants2021

    • Author(s)
      Tsujimura Masaki、Noji Tomoyasu、Saito Keisuke、Kojima Keiichi、Sudo Yuki、Ishikita Hiroshi
    • Journal Title

      Biochimica et Biophysica Acta (BBA) - Bioenergetics

      Volume: 1862 Issue: 2 Pages: 148349-148349

    • DOI

      10.1016/j.bbabio.2020.148349

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] マルチタレント光受容タンパク質「ロドプシン」2021

    • Author(s)
      須藤雄気、小島慧一
    • Journal Title

      現代化学

      Volume: 5 Pages: 50-53

    • Related Report
      2020 Research-status Report
  • [Journal Article] Lokiarchaeota archaeon schizorhodopsin-2 (LaSzR2) is an inward proton pump displaying a characteristic feature of acid-induced spectral blue-shift2020

    • Author(s)
      Kojima Keiichi、Yoshizawa Susumu、Hasegawa Masumi、Nakama Masaki、Kurihara Marie、Kikukawa Takashi、Sudo Yuki
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 20857-20857

    • DOI

      10.1038/s41598-020-77936-9

    • NAID

      120006955824

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Applicability of Styrene-Maleic Acid Copolymer for Two Microbial Rhodopsins, RxR and HsSRI.2020

    • Author(s)
      Tetsuya Ueta, Keiichi Kojima, Tomoya Hino, Mikihiro Shibata, Shingo Nagano, Yuki Sudo*
    • Journal Title

      Biophysical journal

      Volume: 119 Issue: 9 Pages: 1760-1770

    • DOI

      10.1016/j.bpj.2020.09.026

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Comparative Studies of the Fluorescence Properties of Microbial Rhodopsins: Spontaneous Emission Versus Photointermediate Fluorescence2020

    • Author(s)
      Kojima Keiichi、Kurihara Rika、Sakamoto Masayuki、Takanashi Tsukasa、Kuramochi Hikaru、Zhang Xiao Min、Bito Haruhiko、Tahara Tahei、Sudo Yuki
    • Journal Title

      The Journal of Physical Chemistry B

      Volume: 124 Issue: 34 Pages: 7361-7367

    • DOI

      10.1021/acs.jpcb.0c06560

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] A unique clade of light-driven proton-pumping rhodopsins evolved in the cyanobacterial lineage2020

    • Author(s)
      Hasegawa Masumi、Hosaka Toshiaki、Kojima Keiichi、Nishimura Yosuke、Nakajima Yu、Kimura-Someya Tomomi、Shirouzu Mikako、Sudo Yuki、Yoshizawa Susumu
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 16752-16752

    • DOI

      10.1038/s41598-020-73606-y

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Diversity and possibility of microbial rhodopsins2022

    • Author(s)
      Sudo Y.
    • Organizer
      The 19th International Conference on Retinal Proteins
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ロドプシンの生物物理化学研究からの挑戦:『光をくすりへ』2022

    • Author(s)
      須藤雄気
    • Organizer
      超異分野学会 東京大会2022
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] ロドプシンエンジニアリングによる生命機能の光制御・操作2022

    • Author(s)
      須藤雄気
    • Organizer
      第22回・日本蛋白質科学会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Optical control of biological functions by microbial rhodopsins2021

    • Author(s)
      Sudo Y.
    • Organizer
      Pacifichem2021
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 光をくすりにする!?―ロドプシンによる生命機能の光操作2021

    • Author(s)
      須藤雄気
    • Organizer
      日本薬学会北海道支部講演会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] Optical control of biological activities with multi-functional photoreactive membrane protein rhodopsin2021

    • Author(s)
      Sudo Y.
    • Organizer
      第59回・日本生物物理学会シンポジウム
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] 『光がくすりになる!?』2021

    • Author(s)
      須藤雄気
    • Organizer
      第42回・生体膜と薬物の相互作用シンポジウム
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] 『光 が くすりになる!?』2020

    • Author(s)
      須藤雄気
    • Organizer
      第8回青陵サイエンストーク
    • Related Report
      2020 Research-status Report
    • Invited
  • [Remarks] 研究室ホームページ

    • URL

      http://www.pharm.okayama-u.ac.jp/lab/bukka/

    • Related Report
      2022 Annual Research Report 2021 Research-status Report 2020 Research-status Report
  • [Patent(Industrial Property Rights)] 光合成生物の形質転換体およびその用途2022

    • Inventor(s)
      須藤雄気、小島慧一
    • Industrial Property Rights Holder
      須藤雄気、小島慧一
    • Industrial Property Rights Type
      特許
    • Filing Date
      2022
    • Related Report
      2022 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 光合成生物の形質転換体およびその用途2021

    • Inventor(s)
      須藤雄気、小島慧一
    • Industrial Property Rights Holder
      須藤雄気、小島慧一
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2021-089800
    • Filing Date
      2021
    • Related Report
      2021 Research-status Report
  • [Patent(Industrial Property Rights)] 光変調装置及び集光装置2021

    • Inventor(s)
      渋川敦史、須藤雄気、ムサクジャング
    • Industrial Property Rights Holder
      渋川敦史、須藤雄気、ムサクジャング
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2021-153788
    • Filing Date
      2021
    • Related Report
      2021 Research-status Report
  • [Patent(Industrial Property Rights)] 光により細胞死を制御する方法2021

    • Inventor(s)
      須藤雄気、小島慧一、中尾新
    • Industrial Property Rights Holder
      須藤雄気、小島慧一、中尾新
    • Industrial Property Rights Type
      特許
    • Filing Date
      2021
    • Related Report
      2021 Research-status Report
    • Overseas
  • [Patent(Industrial Property Rights)] 光により細胞死を制御する方法2020

    • Inventor(s)
      須藤雄気、小島慧一、中尾新
    • Industrial Property Rights Holder
      須藤雄気、小島慧一、中尾新
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2020-196718
    • Filing Date
      2020
    • Related Report
      2020 Research-status Report

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Published: 2020-08-03   Modified: 2024-01-30  

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