Osteosarcoma therapeutic strategy targeting anticancer drug resistance by mitochondrial transfer

• Project/Area Number: 20K21659
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 56:Surgery related to the biological and sensory functions and related fields
• Research Institution: Nara Medical University
• Principal Investigator: 國安 弘基 奈良県立医科大学, 医学部, 教授 (00253055)
• Co-Investigator(Kenkyū-buntansha): 谷 里奈 奈良県立医科大学, 医学部, 講師 (20783872) ; 岸 真五 奈良県立医科大学, 医学部附属病院, 研究員 (50790341)
• Project Period (FY): 2020-07-30 – 2024-03-31
• Project Status: Completed (Fiscal Year 2023)
• Budget Amount: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2022: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2021: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2020: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 骨肉腫 / ミトトランスファー / 薬剤耐性 / 幹細胞性 / NFｋBシグナル / HKGB1 / ミトコンドリア / ミトコンドリアトランスファー / HMGB1 / がん細胞 / ミトコンドリア移行 / 化学療法 / 間葉系幹細胞

Outline of Research at the Start

本研究では、抗がん剤処理を受けた骨肉腫細胞が間葉系幹細胞からミトコンドリア移行を受けることで、細胞死から免れ薬剤耐性を獲得するという現象の機序を解明し、その阻害法を発見することによりより有効な骨肉腫治療を可能にする。骨肉腫は、化学療法や分子標的治療の選択肢は未だに乏しく、手術的病変摘出への根治性の高い要求から機能温存に高いハードルが存在する。化学療法の奏効性を高めることは、骨肉腫に罹患する多くの若年者の身体機能の保全を可能にし、将来の社会的活動を担保することにつながると期待される。

Outline of Annual Research Achievements

本年度は、ヒト骨肉腫細胞を用いて、ミトトランスファーについて検討を行った。HMGB1は核内タンパクであるが、細胞障害に伴い酸化されて放出され、損傷組織での炎症、組織修復に関与する。悪性腫瘍においても抗がん剤治療後のがん組織にMSCを誘引し癌細胞の生存に寄与することが報告されているがその機序は明らかでない。Doxorubicin（DOX）およびCisplatin（CDDP）によって腫瘍細胞から放出される酸化型のHMGB1が、間葉系幹細胞(MSC)から骨肉腫細胞へのmitochondria移行(mitochondria transfer、MT)を誘導し耐性に寄与することを見出した。ヒト骨肉腫細胞SaOS2, U2OSとMSCを共培養し、DXR,CDDPで処理したところMTがcontrolに比べ、優位に誘導された。この時腫瘍内のmitochondria由来活性酸素は減少し、腫瘍細胞の耐性化がみられた。次にHMGB1抗体を作成した。SaOS2, U2OS骨肉腫細胞株を用いてこの抗体がHMGB1を中和するか検討した。骨肉腫細胞株をDOXで処理したところ上清のHMGB1濃度が上昇し、抗体を低下したことからDOX（1μM）によってHMGB1が放出され、抗体で中和されていると考えられた。この抗HMGB1抗体は抗がん剤処理時のMTの誘導を著しく抑制した。またこの際、MSCによるROSの減少、抗がん剤耐性が解除された。Mitochondria transferはdonor細胞のMiro1, Cx43の発現量と相関した。最後にMSCを還元型HMGB1及び酸化型HMGB1で処理したところ酸化型HMGB１でのみMT関連遺伝子の発現上昇が確認された。今回の結果から化学療法時の障害で放出される酸化型HMGB1が、MSCによるMTを誘導し抗がん剤抵抗性に寄与すると考えられた。

Research Products

• [Int'l Joint Research] Nantong University(中国)
• [Int'l Joint Research] Saveetha Dental College(インド)
• [Int'l Joint Research] 南通大学(中国)
• [Int'l Joint Research] 南通大学(中国)
• [Journal Article] Claudin-4: A New Molecular Target for Epithelial Cancer Therapy (2023)
  • Author(s): Fujiwara-Tani Rina、Mori Shiori、Ogata Ruiko、Sasaki Rika、Ikemoto Ayaka、Kishi Shingo、Kondoh Masuo、Kuniyasu Hiroki
  • Journal Title: International Journal of Molecular Sciences Volume: 24 Issue: 6 Pages: 5494-5494
  • DOI: 10.3390/ijms24065494
  • Peer Reviewed / Open Access
• [Journal Article] Short telomere lesions with dysplastic metaplasia histology may represent precancerous lesions of Helicobacter pylori-positive gastric mucosa (2023)
  • Author(s): Fujiwara-Tani R, Takagi T, Mori S, Kishi S, Nishiguchi Y, Sasaki T, Ikeda M, Nagai K, Bhawal UK, Ohmori H, Fujii K, Kuniyasu H
  • Journal Title: International Journal of Molecular Sciences Volume: 24(4) Issue: 4 Pages: 3182-3182
  • DOI: 10.3390/ijms24043182
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 5-Aminolevrinic Acid Exhibits Dual Effects on Stemness in Human Sarcoma Cell Lines under Dark Conditions (2023)
  • Author(s): Horii Shohei、Mori Shiori、Ogata Ruiko、Nukaga Shota、Nishida Ryoichi、Kishi Shingo、Sasaki Rika、Ikemoto Ayaka、Owari Takuya、Maesaka Fumisato、Honoki Kanya、Miyake Makito、Tanaka Yasuhito、Fujimoto Kiyohide、Fujiwara-Tani Rina、Kuniyasu Hiroki
  • Journal Title: International Journal of Molecular Sciences Volume: 24 Issue: 7 Pages: 6189-6189
  • DOI: 10.3390/ijms24076189
  • Peer Reviewed / Open Access
• [Journal Article] Berberine Induces Combined Cell Death in Gastrointestinal Cell Lines (2023)
  • Author(s): Mori Shiori、Fujiwara-Tani Rina、Gyoten Momoko、Nukaga Shota、Sasaki Rika、Ikemoto Ayaka、Ogata Ruiko、Kishi Shingo、Fujii Kiyomu、Kuniyasu Hiroki
  • Journal Title: International Journal of Molecular Sciences Volume: 24 Issue: 7 Pages: 6588-6588
  • DOI: 10.3390/ijms24076588
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Lauric Acid Overcomes Hypoxia-Induced Gemcitabine Chemoresistance in Pancreatic Ductal Adenocarcinoma (2023)
  • Author(s): Takagi Tadataka、Fujiwara-Tani Rina、Mori Shiori、Kishi Shingo、Nishiguchi Yukiko、Sasaki Takamitsu、Ogata Ruiko、Ikemoto Ayaka、Sasaki Rika、Ohmori Hitoshi、Luo Yi、Bhawal Ujjal Kumar、Sho Masayuki、Kuniyasu Hiroki
  • Journal Title: International Journal of Molecular Sciences Volume: 24 Issue: 8 Pages: 7506-7506
  • DOI: 10.3390/ijms24087506
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Role of creatine shuttle in colorectal cancer cells (2023)
  • Author(s): Kita Mayu、Fujiwara-Tani Rina、Kishi Shingo、Mori Shiori、Ohmori Hitoshi、Nakashima Chie、Goto Kei、Sasaki Takamitsu、Fujii Kiyomu、Kawahara Isao、Bhawal Ujjal Kumar、Luo Yi、Kuniyasu Hiroki
  • Journal Title: Oncotarget Volume: 14 Issue: 1 Pages: 485-501
  • DOI: 10.18632/oncotarget.28436
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] ERVK13-1/miR-873-5p/GNMT Axis Promotes Metastatic Potential in Human Bladder Cancer though Sarcosine Production (2023)
  • Author(s): Kishi Shingo、Mori Shiori、Fujiwara-Tani Rina、Ogata Ruiko、Sasaki Rika、Ikemoto Ayaka、Goto Kei、Sasaki Takamitsu、Miyake Makito、Sasagawa Satoru、Kawaichi Masashi、Luo Yi、Bhawal Ujjal Kumar、Fujimoto Kiyohide、Nakagawa Hidemitsu、Kuniyasu Hiroki
  • Journal Title: International Journal of Molecular Sciences Volume: 24 Issue: 22 Pages: 16367-16367
  • DOI: 10.3390/ijms242216367
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Lovastatin Treatment Inducing Apoptosis in Human Pancreatic Cancer Cells by Inhibiting Cholesterol Rafts in Plasma Membrane and Mitochondria (2023)
  • Author(s): Gyoten Momoko、Luo Yi、Fujiwara-Tani Rina、Mori Shiori、Ogata Ruiko、Kishi Shingo、Kuniyasu Hiroki
  • Journal Title: International Journal of Molecular Sciences Volume: 24 Issue: 23 Pages: 16814-16814
  • DOI: 10.3390/ijms242316814
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Suppressive GLI2 fragment enhances liver metastasis in colorectal cancer (2022)
  • Author(s): Ogata Ruiko、Mori Shiori、Ohmori Hitoshi、Kishi Shingo、Fujiwara-Tani Rina、Sasaki Takamitsu、Nishiguchi Yukiko、Nakashima Chie、Goto Kei、Kawahara Isao、Luo Yi、Kuniyasu Hiroki
  • Journal Title: Oncotarget Volume: 13 Issue: 1 Pages: 122-135
  • DOI: 10.18632/oncotarget.28170
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Sunitinib and Pterostilbene Combination Treatment Exerts Antitumor Effects in Gastric Cancer via Suppression of PDZD8 (2022)
  • Author(s): Hojo Yudai、Kishi Shingo、Mori Shiori、Fujiwara-Tani Rina、Sasaki Takamitsu、Fujii Kiyomu、Nishiguchi Yukiko、Nakashima Chie、Luo Yi、Shinohara Hisashi、Kuniyasu Hiroki
  • Journal Title: International Journal of Molecular Sciences Volume: 23 Issue: 7 Pages: 4002-4002
  • DOI: 10.3390/ijms23074002
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Gemcitabine Resistance in Pancreatic Ductal Carcinoma Cell Lines Stems from Reprogramming of Energy Metabolism (2022)
  • Author(s): Fujiwara-Tani Rina、Sasaki Takamitsu、Takagi Tadataka、Mori Shiori、Kishi Shingo、Nishiguchi Yukiko、Ohmori Hitoshi、Fujii Kiyomu、Kuniyasu Hiroki
  • Journal Title: International Journal of Molecular Sciences Volume: 23 Issue: 14 Pages: 7824-7824
  • DOI: 10.3390/ijms23147824
  • Peer Reviewed / Open Access
• [Journal Article] 5-Aminolevulinic acid overcomes hypoxia-induced radiation resistance by enhancing mitochondrial reactive oxygen species production in prostate cancer cells (2022)
  • Author(s): Owari Takuya、Tanaka Nobumichi、Nakai Yasushi、Miyake Makito、Anai Satoshi、Kishi Shingo、Mori Shiori、Fujiwara-Tani Rina、Hojo Yudai、Mori Takuya、Kuwada Masaomi、Fujii Tomomi、Hasegawa Masatoshi、Fujimoto Kiyohide、Kuniyasu Hiroki
  • Journal Title: British Journal of Cancer Volume: - Issue: 2 Pages: 350-363
  • DOI: 10.1038/s41416-022-01789-4
  • Peer Reviewed / Open Access
• [Journal Article] Oxidized high mobility group B‐1 enhances metastability of colorectal cancer via modification of mesenchymal stem/stromal cells (2022)
  • Author(s): Kishi Shingo、Fujiwara‐Tani Rina、Honoki Kanya、Sasaki Rika、Mori Shiori、Ohmori Hitoshi、Sasaki Takamitsu、Miyagawa Yoshihiro、Kawahara Isao、Kido Akira、Tanaka Yasuhito、Kuniyasu Hiroki
  • Journal Title: Cancer Science Volume: 113 Issue: 8 Pages: 2904-2915
  • DOI: 10.1111/cas.15400
  • Peer Reviewed / Open Access
• [Journal Article] An Axis between the Long Non-Coding RNA HOXA11-AS and NQOs Enhances Metastatic Ability in Oral Squamous Cell Carcinoma (2022)
  • Author(s): Nakashima Chie、Fujiwara-Tani Rina、Mori Shiori、Kishi Shingo、Ohmori Hitoshi、Fujii Kiyomu、Mori Takuya、Miyagawa Yoshihiro、Yamamoto Kazuhiko、Kirita Tadaaki、Luo Yi、Kuniyasu Hiroki
  • Journal Title: International Journal of Molecular Sciences Volume: 23 Issue: 18 Pages: 10704-10704
  • DOI: 10.3390/ijms231810704
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The sustaining of fluorescence in photodynamic diagnosis after the administration of 5-aminolevulinic acid in carcinogen-induced bladder cancer orthotopic rat model and urothelial cancer cell lines (2021)
  • Author(s): Owari Takuya、Iwamoto Takashi、Anai Satoshi、Miyake Makito、Nakai Yasushi、Hori Shunta、Hara Takeshi、Ishii Takuya、Ota Urara、Torimoto Kazumasa、Kuniyasu Hiroki、Fujii Tomomi、Tanaka Nobumichi、Fujimoto Kiyohide
  • Journal Title: Photodiagnosis and Photodynamic Therapy Volume: 34 Pages: 102309-102309
  • DOI: 10.1016/j.pdpdt.2021.102309
  • Peer Reviewed
• [Journal Article] Role of Glycated High Mobility Group Box-1 in Gastric Cancer (2021)
  • Author(s): Kishi Shingo、Nishiguchi Yukiko、Honoki Kanya、Mori Shiori、Fujiwara-Tani Rina、Sasaki Takamitsu、Fujii Kiyomu、Kawahara Isao、Goto Kei、Nakashima Chie、Kido Akira、Tanaka Yasuhito、Luo Yi、Kuniyasu Hiroki
  • Journal Title: International Journal of Molecular Sciences Volume: 22 Issue: 10 Pages: 5185-5185
  • DOI: 10.3390/ijms22105185
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Safety and efficacy of an anti-claudin-5 monoclonal antibody to increase blood-brain barrier permeability for drug delivery to the brain in a non-human primate (2021)
  • Author(s): Tachibana K, Hashimoto Y, Shirakura K, Okada Y, Hirayama R, Iwashita Y, Nishino I, Ago Y, Takeda H, Kuniyasu H, Kondoh M
  • Journal Title: Journal of Controlled Release Volume: 336 Pages: 105-111
  • DOI: 10.1016/j.jconrel.2021.06.009
  • Peer Reviewed
• [Journal Article] Enhancement of Anti-Tumoral Immunity by β-Casomorphin-7 Inhibits Cancer Development and Metastasis of Colorectal Cancer (2021)
  • Author(s): Mori Shiori、Fujiwara-Tani Rina、Kishi Shingo、Sasaki Takamitsu、Ohmori Hitoshi、Goto Kei、Nakashima Chie、Nishiguchi Yukiko、Kawahara Isao、Luo Yi、Kuniyasu Hiroki
  • Journal Title: International Journal of Molecular Sciences Volume: 22 Issue: 15 Pages: 8232-8232
  • DOI: 10.3390/ijms22158232
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] BRAF Mutation Is Associated with Hyperplastic Polyp-Associated Gastric Cancer (2021)
  • Author(s): Fujiwara-Tani Rina、Okamoto Ayaka、Katsuragawa Hiroyuki、Ohmori Hitoshi、Fujii Kiyomu、Mori Shiori、Kishi Shingo、Sasaki Takamitsu、Nakashima Chie、Kawahara Isao、Hojo Yudai、Nishiguchi Yukiko、Mori Takuya、Mizumoto Takeshi、Nagai Kenta、Luo Yi、Kuniyasu Hiroki
  • Journal Title: International Journal of Molecular Sciences Volume: 22 Issue: 23 Pages: 12724-12724
  • DOI: 10.3390/ijms222312724
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Linoleic Acid Upregulates Microrna-494 to Induce Quiescence in Colorectal Cancer (2021)
  • Author(s): Ogata Ruiko、Mori Shiori、Kishi Shingo、Sasaki Rika、Iwata Naoya、Ohmori Hitoshi、Sasaki Takamitsu、Nishiguchi Yukiko、Nakashima Chie、Goto Kei、Kawahara Isao、Fujiwara-Tani Rina、Kuniyasu Hiroki
  • Journal Title: International Journal of Molecular Sciences Volume: 23 Issue: 1 Pages: 225-225
  • DOI: 10.3390/ijms23010225
  • Peer Reviewed / Open Access
• [Journal Article] Targeting claudin‐4 enhances chemosensitivity in breast cancer (2020)
  • Author(s): Luo Yi、Kishi Shingo、Sasaki Takamitsu、Ohmori Hitoshi、Fujiwara‐Tani Rina、Mori Shiori、Goto Kei、Nishiguchi Yukiko、Mori Takuya、Kawahara Isao、Kondoh Masuo、Kuniyasu Hiroki
  • Journal Title: Cancer Science Volume: 111 Issue: 5 Pages: 1840-1850
  • DOI: 10.1111/cas.14361
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Evaluation of cancer-derived myocardial impairments using a mouse model (2020)
  • Author(s): Miyagawa Yoshihiro、Nukaga Shota、Mori Takuya、Fujiwara-Tani Rina、Fujii Kiyomu、Mori Shiori、Goto Kei、Kishi Shingo、Sasaki Takamitsu、Nakashima Chie、Ohmori Hitoshi、Kawahara Isao、Luo Yi、Kuniyasu Hiroki
  • Journal Title: Oncotarget Volume: 11 Issue: 41 Pages: 3712-3722
  • DOI: 10.18632/oncotarget.27759
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Anti-stem cell property of pterostilbene in gastrointestinal cancer cells. (2020)
  • Author(s): Mori S, Kishi S, Honoki K, Fujiwara-Tani R, Moriguchi T, Sasaki T, Fujii K, Tsukamoto S, Fujii H, Kido A, Tanaka Y, Luo Y, Kuniyasu H.
  • Journal Title: Int J Mol Sci. Volume: 21 Issue: 24 Pages: 9347-9347
  • DOI: 10.3390/ijms21249347
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Effect of Proton Pump Inhibitors on Colorectal Cancer (2020)
  • Author(s): Sasaki Takamitsu、Mori Shiori、Kishi Shingo、Fujiwara-Tani Rina、Ohmori Hitoshi、Nishiguchi Yukiko、Hojo Yudai、Kawahara Isao、Nakashima Chie、Fujii Kiyomu、Luo Yi、Kuniyasu Hiroki
  • Journal Title: International Journal of Molecular Sciences Volume: 21 Issue: 11 Pages: 3877-3877
  • DOI: 10.3390/ijms21113877
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Role of Nuclear Claudin-4 in Renal Cell Carcinoma (2020)
  • Author(s): Owari Takuya、Sasaki Takamitsu、Fujii Kiyomu、Fujiwara-Tani Rina、Kishi Shingo、Mori Shiori、Mori Takuya、Goto Kei、Kawahara Isao、Nakai Yasushi、Miyake Makito、Luo Yi、Tanaka Nobumichi、Kondoh Masuo、Fujimoto Kiyohide、Kuniyasu Hiroki
  • Journal Title: International Journal of Molecular Sciences Volume: 21 Issue: 21 Pages: 8340-8340
  • DOI: 10.3390/ijms21218340
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Malic enzyme 1 is associated with tumor budding in oral squamous cell carcinomas. (2020)
  • Author(s): Nakashima C, Kirita T, Yamamoto K, Mori S, Luo Y, Sasaki T, Fujii K, Ohmori H, Kawahara I, Mori T, Goto K, Kishi S, Fujiwara-Tani R, Kuniyasu H
  • Journal Title: Int J Mol Sci Volume: 21 Issue: 19 Pages: 7149-7149
  • DOI: 10.3390/ijms21197149
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Ring box protein-1 is associated with a poor prognosis and tumor progression in esophageal cancer (2020)
  • Author(s): Kunishige Tomohiro、Migita Kazuhiro、Matsumoto Sohei、Wakatsuki Kohei、Nakade Hiroshi、Miyao Shintaro、Kuniyasu Hiroki、Sho Masayuki
  • Journal Title: Oncology Letters Volume: 20 Issue: 3 Pages: 2919-2927
  • DOI: 10.3892/ol.2020.11840
  • Peer Reviewed / Open Access
• [Journal Article] Clostridium perfringens enterotoxin induced claudin-4 to activate YAP in oral squamous cell carcinomas. (2020)
  • Author(s): Nakashima C, Yamamoto K, Kishi S, Sasaki T, Ohmori H, Fujiwara-Tani R Mori S, Kawahara I, Nishiguchi Y, Mori T, Kondoh M, Luo Y, Kirita T, Kuniyasu H
  • Journal Title: Oncotarget Volume: 11 Issue: 4 Pages: 309-321
  • DOI: 10.18632/oncotarget.27424
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Diabetes mellitus is associated with liver metastasis of colorectal cancer through production of biglycan-rich cancer stroma (2020)
  • Author(s): Fujiwara-Tani Rina、Sasaki Takamitsu、Fujii Kiyomu、Luo Yi、Mori Takuya、Kishi Shingo、Mori Shiori、Matsushima-Otsuka Sayako、Nishiguchi Yukiko、Goto Kei、Kawahara Isao、Kondoh Masuo、Sho Masayuki、Kuniyasu Hiroki
  • Journal Title: Oncotarget Volume: 11 Issue: 31 Pages: 2982-2994
  • DOI: 10.18632/oncotarget.27674
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Combined administration of lauric acid and glucose improved cancer‐derived cardiac atrophy in a mouse cachexia model (2020)
  • Author(s): Nukaga Shota、Mori Takuya、Miyagawa Yoshihiro、Fujiwara‐Tani Rina、Sasaki Takamitsu、Fujii Kiyomu、Mori Shiori、Goto Kei、Kishi Shingo、Nakashima Chie、Ohmori Hitoshi、Kawahara Isao、Luo Yi、Kuniyasu Hiroki
  • Journal Title: Cancer Science Volume: 111 Issue: 12 Pages: 4605-4615
  • DOI: 10.1111/cas.14656
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Role of Clostridium perfringens Enterotoxin on YAP Activation in Colonic Sessile Serrated Adenoma/Polyps with Dysplasia (2020)
  • Author(s): Fujiwara-Tani Rina、Fujii Kiyomu、Mori Shiori、Kishi Shingo、Sasaki Takamitsu、Ohmori Hitoshi、Nakashima Chie、Kawahara Isao、Nishiguchi Yukiko、Mori Takuya、Sho Masayuki、Kondoh Masuo、Luo Yi、Kuniyasu Hiroki
  • Journal Title: International Journal of Molecular Sciences Volume: 21 Issue: 11 Pages: 3840-3840
  • DOI: 10.3390/ijms21113840
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Safety assessment of modifying the blood-brain barrier by targeting claudin-5 in a cynomolgus monkey model. (2023)
  • Author(s): Kuzu T, Hashimoto Y, Wakayama E, Nishino I, Tachibana K, Okada Y, Hirayama R, Kuniyasu H, Kondoh M
  • Organizer: Society of Toxicology (SOT) 62nd Annual Meeting and ToxExpo, Nashville, Tennessee, USA, 2023.3.19-23
  • Int'l Joint Research
• [Presentation] Lauric acid enhances gemcitabine chemosensitivity by targeting hypoxia-induced mitochondrial dysfunction and stemness in pancreatic ductal adenocarcinoma. (2023)
  • Author(s): Takagi T, Fujiwara-Tani R, Mori S, Sho M, Kuniyasu H
  • Organizer: American Association for Cancer Research Annual Meeting 2023, Orlando, Florida, USA, 2023.4.14-19
  • Int'l Joint Research
• [Presentation] Pterostilbene enhances anticancer effect of lapatinib in gastric cancer cell lines (2023)
  • Author(s): Nishiguchi Y, Rina Fujiwara-Tani, Shiori Mori, Sho M, Hiroki Kuniyasu
  • Organizer: International Gastric Cancer Congress 2023, Yokohama, Japan, 2023.6.14-17
  • Int'l Joint Research
• [Presentation] Caprylic acid alleviates cachexia-induced myocardial damage by inhibiting HMGB1 via ketone body. (2023)
  • Author(s): Nukaga S, Rina Fujiwara-Tani R, Miyagawa Y, Kawahara I, Nishida R, Mori T, Goto K, Kishi S, Mori S, Fujii K, Ohmori H, Kuniyasu H
  • Organizer: MASCC 2023 Annual Meeting, Nara, Japan, 2023.6.22-24
  • Int'l Joint Research
• [Presentation] Lovastatin induces novel cell death due to impaired mitochondria iron utilization specifically in osteosarcoma cells. (2023)
  • Author(s): Kishi S, Mori S, Honoki K, Fujii H, Tsukamoto S, Sasagawa S, Nakagawa H, Kuniyasu H, Tanaka Y
  • Organizer: Annual Meeting of Connective Tissue Oncology Society, Dublin, Ireland, 2023.11.1-4
  • Int'l Joint Research
• [Presentation] Safety assessment of modifying the blood-brain barrier by targeting claudin-5 in a cynomolgus monkey model. (2023)
  • Author(s): Kuzu T, Hashimoto Y, Wakayama E, Nishino I, Tachibana K, Okada Y, Hirayama R, Kuniyasu H, Kondoh M
  • Organizer: Society of Toxicology (SOT) 62nd Annual Meeting and ToxExpo
  • Int'l Joint Research
• [Presentation] Lauric acid enhances gemcitabine chemosensitivity by targeting hypoxia-induced mitochondrial dysfunction and stemness in pancreatic ductal adenocarcinoma. (2023)
  • Author(s): Takagi T, Fujiwara-Tani R, Mori S, Sho M, Kuniyasu H
  • Organizer: American Association for Cancer Research Annual Meeting 2023
  • Int'l Joint Research
• [Presentation] Pterostilbene enhances anticancer effect of lapatinib in gastric cancer cell lines. (2023)
  • Author(s): Nishiguchi Y, Rina Fujiwara-Tani, Shiori Mori, Sho M, Hiroki Kuniyasu
  • Organizer: International Gastric Cancer Congress 2023, Yokohama, Japan, 2023.6.14-17
  • Int'l Joint Research
• [Presentation] 酸化型HMGB1による間葉系幹細胞からのミトコンドリア移行は大腸癌細胞の薬剤耐性をもたらす (2023)
  • Author(s): 佐々木里歌、岸真五、森汐莉、谷里奈、國安弘基
  • Organizer: 第112回日本病理学会総会
• [Presentation] Dual inhibition of direct metabolic features targets osteosarcoma stem cells. (2021)
  • Author(s): Kishi S, Kanya Honoki K, Mori S, Kuniyasu H, Tanaka Y
  • Organizer: Asia Pacific Musculoskeletal Tumor Society 2021
  • Int'l Joint Research
• [Presentation] PterostilbeneはF0-F1ATPase阻害作用をもち骨肉腫細胞特異的にmitochondria毒性をもたらす (2021)
  • Author(s): 岸 真五、森 汐莉、朴木寛弥、谷 里奈、大森 斉、藤井 澄、佐々木里歌、藤井宏真、塚本真治、田中康仁、國安弘基
  • Organizer: 第110回日本病理学会総会
• [Presentation] HMGB1は間葉系幹細胞から5-fluorouracilにより損傷を受けた癌細胞へのミトコンドリア輸送を制御する (2021)
  • Author(s): 佐々木里歌、岸 真五、森 汐莉、谷 里奈、佐々木隆光、羅 奕、國安弘基
  • Organizer: 第110回日本病理学会総会
• [Presentation] Oxidized HMGB1 induces drug-resistance of colon cancer cells through mitochondrial transfer from mesenchymal stem cells. (2021)
  • Author(s): Sasaki R, Kishi S, Mori S, Fujiwara-Tani R, Kuniyasu H
  • Organizer: 80th Annual Meeting of Japanese Association for Cancer
• [Presentation] F0-F1 ATPase is a therapeutic target for osteosarcoma without cardiotoxicity. (2021)
  • Author(s): Kishi S, Honoki K, Mori S, Fujiwara-Tani R, Kido A, Fujii H, Tsukamoto S, Tanaka Y, Kuniyasu H
  • Organizer: 80th Annual Meeting of Japanese Association for Cancer
• [Presentation] がん悪液質における心筋ミトコンドリア機能障害に対する中鎖脂肪酸の影響 (2020)
  • Author(s): 額賀翔太、大森 斉、森 拓也、宮川良博、後藤 桂、川原 勲、谷 里奈、岸 真吾、森 汐莉、國安弘基
  • Organizer: 第109回日本病理学会総会
• [Presentation] 小胞体－ミトコンドリア繋留分子PDZD8の抑制は胃癌に細胞死をもたらす (2020)
  • Author(s): 北條雄大、岸 真五、森 汐莉、谷 里奈、篠原 尚、國安弘基
  • Organizer: 第109回日本病理学会総会
• [Presentation] HMGB1は間葉系幹細胞から5-fluorouracilにより損傷を受けた癌細胞へのミトコンドリア輸送を制御する (2020)
  • Author(s): 佐々木里歌、岸真吾、森汐莉、谷 里奈、國安弘基
  • Organizer: 第109回日本病理学会総会
• [Presentation] PDZD8による小胞体-ミトコンドリアの繋留は骨肉腫細胞においてミトコンドリアの活性を制御する (2020)
  • Author(s): 岸真吾、森汐莉, 谷里奈, 森拓也, 宮川良博, 額賀翔太, 川原勲, 羅奕, 朴木寛容, 國安弘基
  • Organizer: 第109回日本病理学会総会
• [Presentation] HMGB1 regulates mitochondrial transfer from mesenchymal stem cells to cancer cells damaged by 5-fluorouracil (2020)
  • Author(s): Sasaki R, Kishi S, Mori S, Fijiwara-Tani R, Sasaki T, Luo Y, Kuniyasu H
  • Organizer: 79th Annual Meeting of Japanese Association for Cancer
• [Presentation] Mitochondria metabolism is correlated with gemcitabine resistance in MIA-PaCa-2. (2020)
  • Author(s): Fujiwara-Tani R, Sasaki T, Mori S, Kishi S, Kuniyasu H
  • Organizer: 79th Annual Meeting of Japanese Association for Cancer
• [Presentation] The creatine kinase is a metabolic vulnerability in Osteosarcoma. (2020)
  • Author(s): Kishi S, Mori S, Fujiwara-Tani R, Hojo Y, Owar T, Mori T, Nukaga S, Kita M, Sasaki R, Honoki K, Kuniyasu H
  • Organizer: 79th Annual Meeting of Japanese Association for Cancer
• [Presentation] Effects of medium-chain fatty acids on cancer-derived myocardial atrophy (2020)
  • Author(s): Nukaga S, Mori T, Luo Y, Kawahara I, Miyagawa Y, Goto K, Fujiwara-Tani R, Kishi S, Mori S, Ohmori H, Kuniyasu H
  • Organizer: 79th Annual Meeting of Japanese Association for Cancer
• [Book] Claudin-4 as a molecular target for cancer therapy. (2023)
  • Author(s): Fujiwara-Tani R, Mori S, Ogata R, Sasaki R, Ikemoto A, Kishi S, Kondoh M, Kuniyasu H
  • Publisher: MDPI
• [Remarks] 奈良県立医科大学分子病理学講座
  • URL: https://molepath.naramed-u.ac.jp/
• [Remarks] 奈良県立医科大学分子病理学講座
  • URL: http://www.naramed-u.ac.jp/~molepath/
• [Remarks] 奈良県立医科大学分子病理学
  • URL: http://www.naramed-u.ac.jp/~molepath/