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Identification of genomic radiation signature using long-read sequencing

Research Project

Project/Area Number 20K21718
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 58:Society medicine, nursing, and related fields
Research InstitutionNagasaki University

Principal Investigator

MITSUTAKE Norisato  長崎大学, 原爆後障害医療研究所, 教授 (50404215)

Co-Investigator(Kenkyū-buntansha) 鈴木 啓司  長崎大学, 原爆後障害医療研究所, 准教授 (00196809)
荻 朋男  名古屋大学, 環境医学研究所, 教授 (80508317)
Project Period (FY) 2020-07-30 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2021: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2020: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords放射線 / ゲノム / HPRT変異クローン / HPRTクローン
Outline of Research at the Start

放射線被ばくによって癌が発生するのは明らかであるが、あるひとつの癌が放射線で引き起こされたものか、その他の原因で起きたものかを区別する方法は今のところない。放射線はクラスター損傷と呼ばれる複雑な異常をDNAに引き起こし、マイクロホモロジーを伴う欠失や逆位を生じさせるとされるが、これらを全ゲノムレベルで解析する手法は今までなかった。本研究では、様々な線量の放射線を照射し樹立したHPRT変異細胞クローンと、新規技術であるロングリードシークエンシングを用い、上記の欠失や逆位を全ゲノムレベルで解析し、放射線刻印とも呼べる放射線特異的なゲノム変化の有無を明らかにする。

Outline of Final Research Achievements

Two different platforms of next generation sequencing, short- and long-read sequencing, revealed the following characteristics of radiation-induced genomic variants in human normal cells: 1) Deletions of various sizes increased in a dose-dependent manner. 2) At high doses, very long deletions (hundreds of thousands to megabases or more) occurred. 3) Many of the deletions had no homology sequence at break points. Deletions by high doses often contained a distinct sequence at the junction. 4) Single nucleotide substitutions also increased but were not dose dependent. 5) Single nucleotide substitutions contained mutational signatures suggestive of oxidative damage. However, we were unable to identify mutations that were completely specific to radiation exposure.

Academic Significance and Societal Importance of the Research Achievements

DNA配列決定法の技術的な進歩により、DNAの配列変化をゲノムワイドに調べることが可能となってきた。放射線被ばくは、発がんリスクを上昇させることは疫学的に明らかであり、放射線によるゲノム変異がその誘因であると考えられている。本研究において、ヒト正常培養細胞で、放射線によって生じるゲノム変異の特徴の多くが詳細に明らかになった。これらの異常が、発癌の第一歩となる可能性が高い。本研究成果は、放射線による生物学的影響、遺伝子異常、発癌などの他の研究にも活かせる基盤的データとなると思われる。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (30 results)

All 2023 2022 2021 2020

All Journal Article (19 results) (of which Int'l Joint Research: 9 results,  Peer Reviewed: 19 results,  Open Access: 14 results) Presentation (11 results) (of which Invited: 7 results)

  • [Journal Article] Technical Report: A Simple and Robust Real-Time Quantitative PCR Method for the Detection of Radiation-Induced Multiple Exon Deletions of the Human HPRT Gene2023

    • Author(s)
      Kawamura Kasumi、Suzuki Keiji、Mitsutake Norisato
    • Journal Title

      Radiation Research

      Volume: 199 Issue: 1 Pages: 83-88

    • DOI

      10.1667/rade-21-00047.1

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of radiation-induced micronuclei associated with premature senescence, and their selective removal by senolytic drug, ABT-2632022

    • Author(s)
      Suzuki Keiji、Kawamura Kasumi、Ujiie Risa、Nakayama Takahumi、Mitsutake Norisato
    • Journal Title

      Mutation Research/Genetic Toxicology and Environmental Mutagenesis

      Volume: 876-877 Pages: 503448-503448

    • DOI

      10.1016/j.mrgentox.2022.503448

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Clinicopathological Implications of the BRAFV600E Mutation in Papillary Thyroid Carcinoma of Ukrainian Patients Exposed to the Chernobyl Radiation in Childhood: A Study for 30 Years After the Accident2022

    • Author(s)
      Zurnadzhy L, Bogdanova T, Rogounovitch TI, Ito M, Tronko M, Yamashita S, Mitsutake N, Chernyshov S, Masiuk S, Saenko VA.
    • Journal Title

      Front. Med., 26 April 2022

      Volume: - Pages: 882727-882727

    • DOI

      10.3389/fmed.2022.882727

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The high degree of similarity in histopathological and clinical characteristics between radiogenic and sporadic papillary thyroid microcarcinomas in young patients2022

    • Author(s)
      Bogdanova Tetiana、Chernyshov Serhii、Zurnadzhy Liudmyla、Rogounovitch Tatiana I.、Mitsutake Norisato、Tronko Mykola、Ito Masahiro、Bolgov Michael、Masiuk Sergii、Yamashita Shunichi、Saenko Vladimir A.
    • Journal Title

      Frontiers in Endocrinology

      Volume: 13 Pages: 970682-970682

    • DOI

      10.3389/fendo.2022.970682

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Aicardi-Goutieres syndrome with SAMHD1 deficiency can be diagnosed by unscheduled DNA synthesis test2022

    • Author(s)
      Senju C, Nakazawa Y, Shimada M, Iwata D, Matsuse M, Tanaka K, Miyazaki Y, Moriwaki S, Mitsutake N, Ogi T.
    • Journal Title

      Frontiers in Pediatrics

      Volume: 10 Pages: 1048002-1048002

    • DOI

      10.3389/fped.2022.1048002

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Anti-CENP-C Antibody-Based Immunofluorescence Dicentric Assay: Radiation Dose-Response, Validation Studies, and Radiation Dose-Dependency on Sister Centromere Fluorescence2022

    • Author(s)
      Ujiie Risa、Kawamura Kasumi、Yamashita Shunichi、Mitsutake Norisato、Suzuki Keiji
    • Journal Title

      Radiation Research

      Volume: 199 Issue: 1 Pages: 74-82

    • DOI

      10.1667/rade-22-00050.1

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The relationship of the clinicopathological characteristics and treatment results of post-Chornobyl papillary thyroid microcarcinomas with the latency period and radiation exposure2022

    • Author(s)
      Bogdanova Tetiana、Chernyshov Serhii、Zurnadzhy Liudmyla、Rogounovitch Tatiana I.、Mitsutake Norisato、Tronko Mykola、Ito Masahiro、Bolgov Michael、Masiuk Sergii、Yamashita Shunichi、Saenko Vladimir A.
    • Journal Title

      Frontiers in Endocrinology

      Volume: 13 Pages: 1078258-1078258

    • DOI

      10.3389/fendo.2022.1078258

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Recent advances in radiobiology with respect to pleiotropic aspects of tissue reaction2021

    • Author(s)
      Suzuki Keiji、Amrenova Aidana、Mitsutake Norisato
    • Journal Title

      Journal of Radiation Research

      Volume: 62 Issue: Supplement_1 Pages: i30-i35

    • DOI

      10.1093/jrr/rraa086

    • NAID

      120007163282

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comprehensive analysis for detecting radiation-specific molecules expressed during radiation-induced rat thyroid carcinogenesis.2021

    • Author(s)
      Kurohama H, Matsuda K, Kishino M, Yoshino M, Yamaguchi Y, Matsuu-Matsuyama M, Kondo H, Mitsutake N, Kinoshita A, Yoshiura KI, Nakashima M.
    • Journal Title

      J Radiat Res.

      Volume: 62 Issue: Supplement_1 Pages: i78-i87

    • DOI

      10.1093/jrr/rraa139

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Molecular pathogenesis of pediatric thyroid carcinoma2021

    • Author(s)
      Mitsutake Norisato、Saenko Vladimir
    • Journal Title

      Journal of Radiation Research

      Volume: 62 Issue: Supplement_1 Pages: i71-i77

    • DOI

      10.1093/jrr/rraa096

    • NAID

      120007163284

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Cell competition between anaplastic thyroid cancer and normal thyroid follicular cells exerts reciprocal stress response defining tumor suppressive effects of normal epithelial tissue2021

    • Author(s)
      Amrenova A, Suzuki K, Saenko V, Yamashita S, Mitsutake N.
    • Journal Title

      PLoS One

      Volume: 4 Issue: 4 Pages: 1-10

    • DOI

      10.1371/journal.pone.0249059

    • NAID

      120007150307

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Major Oncogenic Drivers and Their Clinicopathological Correlations in Sporadic Childhood Papillary Thyroid Carcinoma in Belarus2021

    • Author(s)
      Rogounovitch Tatiana I.、Mankovskaya Svetlana V.、Fridman Mikhail V.、Leonova Tatiana A.、Kondratovitch Victor A.、Konoplya Natalya E.、Yamashita Shunichi、Mitsutake Norisato、Saenko Vladimir A.
    • Journal Title

      Cancers

      Volume: 13 Issue: 13 Pages: 3374-3374

    • DOI

      10.3390/cancers13133374

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Preoperative detection of the TERT promoter mutations in papillary thyroid carcinomas2021

    • Author(s)
      Nakao T, Matsuse M, Saenko V, Rogounovitch T, Tanaka A, Suzuki K, Higuchi M, Sasai H, Sano T, Hirokawa M, Miyauchi A, Kawakami A, Mitsutake N.
    • Journal Title

      Clinical Endocrinology

      Volume: 95 Issue: 5 Pages: 790-799

    • DOI

      10.1111/cen.14567

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] The BRAF V600E Mutation Is Not a Risk Factor for More Aggressive Tumor Behavior in Radiogenic and Sporadic Papillary Thyroid Carcinoma at a Young Age.2021

    • Author(s)
      Zurnadzhy L, Bogdanova T, Rogounovitch TI, Ito M, Tronko M, Yamashita S, Mitsutake N, Chernyshov S, Masiuk S, Saenko VA.
    • Journal Title

      Cancers (Basel).

      Volume: 13 Issue: 23 Pages: 6038-6038

    • DOI

      10.3390/cancers13236038

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Position paper from the Japan Thyroid Association task force on the management of low-risk papillary thyroid microcarcinoma (T1aN0M0) in adults2021

    • Author(s)
      Kazuhiko Horiguchi, Yusaku Yoshida, Kenji Iwaku, Naoya Emoto, Toshihiko Kasahara, Junichiro Sato, Hiroki Shimura, Hisakazu Shindo, Satoru Suzuki, Hidekazu Nagano, Fumihiko Furuya, Noriko Makita, Fumihiko Matsumoto, Katsunori Manaka, Norisato Mitsutake, Megumi Miyakawa, Susumu Yokoya and Iwao Sugitani
    • Journal Title

      Endocrine Journal

      Volume: 68 Issue: 7 Pages: 763-780

    • DOI

      10.1507/endocrj.EJ20-0692

    • NAID

      130008068266

    • ISSN
      0918-8959, 1348-4540
    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Papillary thyroid carcinoma in Ukraine after Chernobyl and in Japan after Fukushima: different histopathological scenarios.2020

    • Author(s)
      Bogdanova TI, Saenko VA, Hashimoto Y, Hirokawa M, Zurnadzhy LY, Hayashi T, Ito M, Iwadate M, Mitsutake N, Rogounovitch T, Sakamoto A, Naganuma H, Miyauchi A, Tronko MD, Thomas G, Yamashita S, Suzuki S:
    • Journal Title

      Thyroid

      Volume: 308 Issue: 9 Pages: 1-13

    • DOI

      10.1089/thy.2020.0308

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The Clinicopathological Results of Thyroid Cancer With BRAFV600E Mutation in the Young Population of Fukushima.2020

    • Author(s)
      Iwadate M, Mitsutake N, Matsuse M, Fukushima T, Suzuki S, Matsumoto Y, Ookouchi C, Mizunuma H, Nakamura I, Nakano K, Sakamoto A, Hirokawa M, Ito M, Naganuma H, Hashimoto Y, Shimura H, Yamashita S, Suzuki S
    • Journal Title

      J Clin Endocrinol Metab

      Volume: 105 Issue: 12 Pages: 573-573

    • DOI

      10.1210/clinem/dgaa573

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Digenic mutations in ALDH2 and ADH5 impair formaldehyde clearance and cause a multisystem disorder, AMeD syndrome2020

    • Author(s)
      Oka Y, Hamada M, Nakazawa Y, ..., Tanaka M, Suganami T, Nakada S, Mitsutake N, Hara Y, Kato K, ..., Kaneko H, Matsumoto N, Matsuda F, Matsuo K, Takahashi Y, Mashimo T, Kojima S, Ogi T
    • Journal Title

      Science Advances

      Volume: 6 Issue: 51 Pages: 7197-7197

    • DOI

      10.1126/sciadv.abd7197

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Targeted Nuclear Irradiation with an X-Ray Microbeam Enhances Total JC-1 Fluorescence from Mitochondria2020

    • Author(s)
      Kaminaga K, Hamada R, Usami N, Suzuki K, Yokoya A
    • Journal Title

      Radiation Research

      Volume: 194 Issue: 5 Pages: 511-518

    • DOI

      10.1667/rr15110.1

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Presentation] 放射線照射によるHPRT変異クローンのロングリード解析2022

    • Author(s)
      光武 範吏、鈴木 啓司、河村 河村 香寿美、三嶋 博之、吉浦 孝一郎
    • Organizer
      日本放射線影響学会第65回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] TERTと甲状腺癌の高度悪性化2021

    • Author(s)
      光武 範吏
    • Organizer
      第94回日本内分泌学会学術総会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] 小児・若年者甲状腺癌の遺伝子解析2021

    • Author(s)
      光武 範吏、松瀬美智子、岩館 学、サエンコ ウラジミール、鈴木 眞一、山下 俊一
    • Organizer
      第34回日本臨床内科医学会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] TERTの転写制御の違いと甲状腺癌悪性度との関連2021

    • Author(s)
      松瀬美智子、酒匂あやか、サエンコ ウラジミール、山下俊一、光武範吏
    • Organizer
      第64回日本甲状腺学会学術集会
    • Related Report
      2021 Research-status Report
  • [Presentation] 甲状腺乳頭癌におけるTERT mRNAのスプライシングバリアントとTERTプロモーター変異・臨床病理学的指標との関連2021

    • Author(s)
      酒匂あやか、松瀬美智子、サエンコ ウラジミール、西原永潤、廣川満良、宮内昭、川上純、光武範吏
    • Organizer
      第64回日本甲状腺学会学術集会
    • Related Report
      2021 Research-status Report
  • [Presentation] 増悪する甲状腺乳頭癌を予測できる分子マーカー:さらなる高精度化と細胞診への応用2020

    • Author(s)
      松瀬 美智子, Vladimir Saenko, 中尾 朋恵, 山下 俊一, 光武 範吏
    • Organizer
      第63回 日本甲状腺学会学術総会
    • Related Report
      2020 Research-status Report
  • [Presentation] 遺伝子変異からみた甲状腺がんの診断:穿刺吸引細胞診におけるTERTプロモーター変異の検出2020

    • Author(s)
      光武 範吏
    • Organizer
      第30回 臨床内分泌代謝Update
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] チェルノブイリと福島の甲状腺がんにおける様々な病理学的シナリオ2020

    • Author(s)
      サエンコ ウラジミール, ボグダノワ タチアナ, 橋本 優子, 廣川 満良, リュドミラ ズルナドジ, 林 俊哲, 伊東 正博, 岩館 学, 光武 範吏, ログノビッチ タチアナ, 坂本 穆彦, 長沼 廣, 宮内 昭, トロンコ ミコラ, トマス ジュラルディン, 山下 俊一, 鈴木 眞一
    • Organizer
      第63回 日本放射線影響学会 年次大会
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] チェルノブイリ原発事故後と福島県における小児・若年者甲状腺癌における遺伝子変異2020

    • Author(s)
      光武 範吏, 松瀬 美智子, 岩館 学, 山下 俊一, サエンコ ウラジミール, 鈴木 眞一
    • Organizer
      第63回 日本放射線影響学会 年次大会
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] 乳頭癌の遺伝子異常2020

    • Author(s)
      光武 範吏
    • Organizer
      第32回 日本内分泌外科学会総会
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] 小児・若年者甲状腺がんと成人甲状腺がんの遺伝子変異の相違2020

    • Author(s)
      光武 範吏, 松瀬 美智子, 岩館 学, サエンコ ウラジミール, 鈴木 眞一, 山下 俊一
    • Organizer
      第93回 日本内分泌学会学術総会
    • Related Report
      2020 Research-status Report
    • Invited

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Published: 2020-08-03   Modified: 2024-01-30  

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