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Development of artificial mitochondria with cell-symbiosis ability

Research Project

Project/Area Number 20K21872
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 90:Biomedical engineering and related fields
Research InstitutionHokkaido University

Principal Investigator

Yamada Yuma  北海道大学, 薬学研究院, 准教授 (60451431)

Project Period (FY) 2020-07-30 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2021: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2020: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords薬物送達システム / ミトコンドリア / 共生 / 移植 / オルガネラ製剤
Outline of Research at the Start

細胞内部の異常ミトコンドリアの割合が増加すると、正常な細胞機能を維持する事が困難となり疾患を発症すると考えらえている。従って、ミトコドリアの質・量を制御する技術はミトコンドリアを標的とした革新的治療法の創出にも貢献する。本申請研究では、細胞内のミトコドリアの質・量を制御することが可能となる、細胞共生能を有する『人工ミトコンドリア』の開発を進める。本研究で開発する人工ミトコンドリアは、オルガネラ製剤という新しい領域を拓き、根本的な治療が難しいミトコンドリア病などの治療に大きく貢献する事が期待される。

Outline of Final Research Achievements

Mitochondria transplantation therapy, in which mitochondria are transplanted into diseased cells, could be a very useful, but there is a problem in that optimal symbiosis (cellular internalization and mitochondrial communication) for therapeutic efficacy has not been achieved. The aim of this research was to develop artificial mitochondria with enhanced symbiotic ability for use in therapeutic applications. This research proposal includes (i) the formulation of artificial mitochondria and the optimization of their intracellular dynamics, (ii) validation of their therapeutic efficiency using a model of mitochondrial disease cells. A number of results regarding the development of artificial mitochondria have been achieved and evaluated in preliminary experiments.

Academic Significance and Societal Importance of the Research Achievements

本研究で開発を進めた人工ミトコンドリアは、オルガネラ製剤という新たな領域を拓き、根本的な治療が難しいミトコンドリア病などの治療に大きく貢献する事が期待される。さらに、組織・個体レベルでの人工ミトコンドリアの移植が実現すれば新たなモデル動物を作出し、ミトコンドリアが関連する個体レベルの生命現象の生体制御研究を世界に先駆けて着手する事が可能になる。本申請研究で得た知見は、我が国の科学技術発展および新薬開発にも大きく貢献するが期待される。

Report

(3 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • Research Products

    (17 results)

All 2022 2021 2020 Other

All Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 3 results) Presentation (9 results) (of which Int'l Joint Research: 3 results,  Invited: 5 results) Remarks (2 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Journal Article] Transplantation of MITO cells, mitochondria activated cardiac progenitor cells, to the ischemic myocardium of mouse enhances the therapeutic effect2022

    • Author(s)
      Daisuke Sasaki, Jiro Abe, Atsuhito Takeda, Hideyoshi Harashima and Yuma Yamada
    • Journal Title

      Scientific Reports

      Volume: 12 Issue: 1 Pages: 4344-4357

    • DOI

      10.1038/s41598-022-08583-5

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Resveratrol-Encapsulated Mitochondria-Targeting Liposome Enhances Mitochondrial Respiratory Capacity in Myocardial Cells2021

    • Author(s)
      Tsujioka Takao、Sasaki Daisuke、Takeda Atsuhito、Harashima Hideyoshi、Yamada Yuma
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 23 Issue: 1 Pages: 112-112

    • DOI

      10.3390/ijms23010112

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Challenges in promoting mitochondrial transplantation therapy.2020

    • Author(s)
      Yamada Y, Ito M, Arai M, Hibino M, Tsujioka T, Harashima H
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 21 Issue: 17 Pages: 6365-6365

    • DOI

      10.3390/ijms21176365

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Power of mitochondrial drug delivery systems to produce innovative nanomedicines2020

    • Author(s)
      Yamada Y, Satrialdi, Hibino M, Sasaki D, Jiro A, Harashima H
    • Journal Title

      Adv. Drug. Deliv. Rev.

      Volume: 154-155 Pages: 187-209

    • DOI

      10.1016/j.addr.2020.09.010

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] ミトコンドリア機能を操作するDrug Delivery System2021

    • Author(s)
      山田 勇磨、原島秀吉
    • Organizer
      第30回日本バイオイメージング学会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Mitochondria-activated myocardial progenitor cell (MITO cell) transplantation therapy in a mouse model of ischemia and reperfusion2021

    • Author(s)
      Sasaki D, Yamada Y, Abe J, Takeda A, Harashima H
    • Organizer
      54th Annual meeting for European Paediatric and Congenital cardiology
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ミトコンドリア運搬用DDS (Trans MIT system)の開発2021

    • Author(s)
      山田勇磨、日比野光恵、伊藤百、荒井愛永、佐々木大輔、真栄城正寿、渡慶次学、太田善浩、原島秀吉
    • Organizer
      日本薬剤学会第36年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ヒトミトコンドリア強化心筋幹細胞を用いた、急性心不全モデルラットに対する心筋幹細胞移植療法の治療効果の検討2021

    • Author(s)
      佐々木大輔、山田勇磨、後藤悠太、白石真大、武田充人、原島秀吉
    • Organizer
      日本薬剤学会第36年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ミトコンドリア人工共生による細胞機能制御の試み2021

    • Author(s)
      山田勇磨、日比野光恵、伊藤百、荒井愛永、佐々木大輔、真栄城正寿、渡慶次学、太田善浩、原島秀吉
    • Organizer
      第37回日本DDS学会学術集会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 希少疾患の未来について考える 患者とともに挑むイノベーション2021

    • Author(s)
      山田 勇磨
    • Organizer
      iPark Frontier Talk
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] Mito-porter, Investigation of Drug Delivery System Targeting Mitochondria.2020

    • Author(s)
      Yamada Y, Harashima H.
    • Organizer
      The 84th annual scinetific meeting of the japanse circulation society
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] ミトコンドリア標的型ナノ医薬品の創製を目指して2020

    • Author(s)
      山田 勇磨
    • Organizer
      第35回日本小児神経学会北海道地方会
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] The challenge of developing mitochondrial nano medicine based on MITO-Porter Technology2020

    • Author(s)
      Yamada Y, Harashima H.
    • Organizer
      JSPCC-AHA 2020
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research / Invited
  • [Remarks] 北海道大学 大学院薬学研究院 薬剤分子設計学研究室HP

    • URL

      https://www.pharm.hokudai.ac.jp/yakusetu/

    • Related Report
      2021 Annual Research Report 2020 Research-status Report
  • [Remarks] 北海道大学 大学院薬学研究院 HP

    • URL

      https://www.pharm.hokudai.ac.jp/

    • Related Report
      2021 Annual Research Report 2020 Research-status Report
  • [Patent(Industrial Property Rights)] 単離ミトコンドリアを内包した脂質膜ベースの小胞2020

    • Inventor(s)
      菅沼正司, 山田勇磨,原島秀吉, 他
    • Industrial Property Rights Holder
      菅沼正司, 山田勇磨,原島秀吉, 他
    • Industrial Property Rights Type
      特許
    • Filing Date
      2020
    • Related Report
      2020 Research-status Report
    • Overseas
  • [Patent(Industrial Property Rights)] 単離ミトコンドリアの再構成用の凍結乾燥製剤2020

    • Inventor(s)
      山田勇磨, 佐々木大輔, 原島秀吉
    • Industrial Property Rights Holder
      山田勇磨, 佐々木大輔, 原島秀吉
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2020-122400
    • Filing Date
      2020
    • Related Report
      2020 Research-status Report

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Published: 2020-08-03   Modified: 2023-01-30  

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