Control of Laminar flow in open space for subcellular operation
| Project/Area Number |
20K22555
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund |
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| Review Section |
0502:Inorganic/coordination chemistry, analytical chemistry, inorganic materials chemistry, energy-related chemistry, and related fields
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| Research Institution | Tokyo Metropolitan University |
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Principal Investigator |
MAO SIFENG 東京都立大学, 都市環境科学研究科, 助教 (40885315)
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| Project Period (FY) |
2020-09-11 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | Microfluidics, / Microchemical Pen, / Laminar flow, / Single cell, / Lactate, / Subcellular / 5-nozzle chemical pen / Laminar flow / Comsol simulation / Cell research / Cell staining / Microfluidics / Subcellular treatment |
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| Outline of Research at the Start |
We will develop new method for generating three-phase laminar flow to confine target reagent in limited region. This confined flow will be used to label desirable organelles, proteins and multiple types of molecules in defined position of a single cell.
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| Outline of Final Research Achievements |
Firstly, we developed a push-pull nozzle system for in situ single-cell stimulation and the real-time electrochemical monitoring of lactate
secreted from the cell. Glucose solution was continuously supplied to the cell and the lactate secreted from the cell was collected and transport to the aspiration channel for electrochemical detection. Rapid and sensitive detection of lactate changes from a single cell was achieved by amperometry. The results was pubilished on Analytical Chemistry.
Then, we developted a five-nozzle chemical pen to explore the communication between cells by precise manipulation of a part of the cell. By controlling the fluid velocity at each nozzle, a thin effective region was formed in the central region. The fluid in the effective region was stable and had high spatial resolutions enough to stimulate and operate a limited region of a single cell without affecting the other parts.
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| Academic Significance and Societal Importance of the Research Achievements |
We developed new technology for cell analysis. It will be a powerful tool for scientists to carry our single cell researches and cell molecular science.
And the achieved single cell analysis will benefit for disease diagnosis, especially for cancer diagnosis.
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Report
(2 results)
Research Products
(1 results)
