| Project/Area Number |
20K22685
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund |
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| Review Section |
0704:Neuroscience, brain sciences, and related fields
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| Research Institution | Kyoto University |
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Principal Investigator |
Liu Pin-Wu 京都大学, 医学研究科, 研究員 (60886563)
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| Project Period (FY) |
2020-09-11 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | Memory formation / Synaptic plasiticity / Super-resolution imaging / STORM / liquid phase separation / synaptic plasticity / memory formation / AMPAR / PSD / super-resolution imaging / Synapse / Synaptic plasticity / Memory / Super resolution / Postsynaptic Density |
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| Outline of Research at the Start |
Efficacy of synaptic transmission is dependent on the localization of synaptic proteins. Thus, elucidating the behavior of synaptic proteins is critical to understand memory formation and related disorders. We recently found that excitatory stimulation modifies protein interactions and re-organizes the sub-synaptic localization of proteins. In this study, we will use super-resolution microscopy in neurons to elucidate how sub-synaptic localization of synaptic proteins is regulated. The result will be a basis to treat memory-related mental disorders and neurodegenerative diseases.
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| Outline of Final Research Achievements |
To investigate the molecular mechanism underlying the memory formation, we focused on elucidating the behavior of synaptic proteins. Previously, we have reported the synaptic activity-dependent organization of nano-scale localization of postsynaptic proteins via liquid-liquid phase separation (LLPS), which explains the potentiation of synapse during memory formation.
To observe the detailed structure of synaptic protein clusters requires super-resolution imaging. Thus, to expand our research, we first set up the super-resolution microscopy with Nikon STORM system. However, to observe the interaction between two synaptic proteins, dual-channel super-resolution microscopy is required. So, we extend our system from single-channel to dual-channel by optimizing imaging buffer, upgrading laser power and modifying the pathway of laser beam. Overall, we established the dual-channel STORM system that allow us to observe the interaction among synaptic proteins at nano-scale level.
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| Academic Significance and Societal Importance of the Research Achievements |
Being able to study the interaction among synaptic proteins at nano-scale level by dual-channel STORM allows us to dig out the mechanism of synaptic plasticity which serves as molecular basis of memory that can lead medical invention of memory-related mental disorders and neurodegenerative diseases.
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