| Project/Area Number |
20K22969
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund |
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| Review Section |
0906:Surgery related to the biological and sensory functions and related fields
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2020-09-11 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | Newt / Regeneration / Scarless / Tissue / Transgenic / newt / regeneration / scarless / physiology / tissue / transgenic |
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| Outline of Research at the Start |
We aim to understand the molecular events (reprogramming/dedifferentiation, patterning, and anti-fibrotic regulation) occurring during early stages of newt tissue regeneration. Here we will develop new transgenic newt lines to study:
1) candidate genes involved in cell reprogramming /dedifferentiation as well as anti-fibrotic regulation in adult RPE cells. 2) Functional analysis of Newtic1 during limb regeneration. 3) Identification of specific cell types regulating Shh expression during limb regeneration patterning.
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| Outline of Final Research Achievements |
The adult newt can regenerate injured tissue without scars. During this startup research the results were the following:
LIMB REGENERATION: (1) Development of transgenic plasmids for Newtic-1 gene knockdown (inhibit), now being tested. (2) Development of an in vivo cellular tracking systems for newt red blood cells and patterning cells.
SCARLESS SKIN: (1) We studied scarless regeneration in different skin areas of the newt, results demonstrate the newt has unique skin cells for scarless healing. (2) We studied limb regeneration following skin grafts reposition, results show that skin maybe involved in limb patterning and finger patterning.
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| Academic Significance and Societal Importance of the Research Achievements |
In society, patients have low ability to regenerate injured tissue. However the adult newt is capable of amazing tissue regeneration. During this startup we established in vivo tracking systems and studied the newts unique skin cells during scarless wound healing and limb regeneration.
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