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Development and application of innovative monitoring technologies of microorganisms: Challenge to eradication of waterborne infectious diseases in Asia

Research Project

Project/Area Number 20KK0090
Research Category

Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 22:Civil engineering and related fields
Research InstitutionHokkaido University

Principal Investigator

Satoh Hisashi  北海道大学, 工学研究院, 教授 (80326636)

Co-Investigator(Kenkyū-buntansha) 齋藤 伸吾  埼玉大学, 理工学研究科, 教授 (60343018)
山村 寛  中央大学, 理工学部, 教授 (40515334)
丁 青  中央大学, 理工学部, 助教 (70837476)
中屋 佑紀  北海道大学, 工学研究院, 助教 (60868735)
Project Period (FY) 2020-10-27 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2023: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2020: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Keywordsセンサ / 酵素活性 / 薬剤耐性菌 / 簡易測定 / 水系感染症 / 微生物モニタリング技術 / 飲料水
Outline of Research at the Start

革新的な3種の微生物モニタリング技術を開発する。これらを使ってスリランカとインドネシアの家庭で一般市民が飲料水中の微生物量を測定できる社会を実現する。日本の使用済み逆浸透膜を再利用し飲料水水質を向上する。これら研究を海外で行うことで、海外の共同研究者と強固な研究ネットワークを構築する。国内外の若手研究者と大学院生が現場で作業を共有し研究ネットワークを持続可能なものとする。

Outline of Final Research Achievements

Three innovative microbial monitoring techniques were developed. These were used to measure the microbial abundance of river water, which is the source of water supply in Sri Lanka. By conducting these studies overseas, we built a solid research network with overseas collaborators. We demonstrated that chlorine disinfection was effective in inactivating microorganisms. A simple technique was developed to determine whether a fecal contamination source was nearby.

Academic Significance and Societal Importance of the Research Achievements

糞便汚染源が近くにあるか否かを簡便に判定する技術を開発した。この技術により、ヒト、モノ、カネがない途上国で簡単に水の衛生学的安全性を判定できるようになり、途上国の人々の疾病を減らし、平均寿命を押し上げることに貢献できる。
海外の共同研究者と強固な研究ネットワークを構築した。これにより今後多くの留学生を日本に受け入れることができ、インドネシアスリランカの水環境保全や上下水道処理技術の高度化に貢献できる。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (27 results)

All 2023 2022 2021 2020 Other

All Int'l Joint Research (4 results) Journal Article (8 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 8 results,  Open Access: 5 results) Presentation (13 results) (of which Int'l Joint Research: 6 results) Remarks (2 results)

  • [Int'l Joint Research] University of Ruhuna(スリランカ)

    • Related Report
      2022 Research-status Report
  • [Int'l Joint Research] Institut Teknologi Bandung(インドネシア)

    • Related Report
      2022 Research-status Report
  • [Int'l Joint Research] University of Ruhuna(スリランカ)

    • Related Report
      2021 Research-status Report
  • [Int'l Joint Research] Institut Teknologi Bandung(インドネシア)

    • Related Report
      2021 Research-status Report
  • [Journal Article] A simple and rapid method for detecting fecal pollution in urban rivers by measuring the intrinsic β-D-glucuronidase activity of Escherichia coli2023

    • Author(s)
      Shayan Mohomed N.M.、Tanaka Yuna、Hirano Reiko、Nakaya Yuki、Satoh Hisashi
    • Journal Title

      Water Research

      Volume: 246 Pages: 120689-120689

    • DOI

      10.1016/j.watres.2023.120689

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Separate determination of arsenite and arsenate in groundwater samples by simple analytical methods2022

    • Author(s)
      Matsunaga Koji、Nakaya Yuki、Satoh Hisashi
    • Journal Title

      Water Supply

      Volume: 22 Issue: 10 Pages: 7635-7642

    • DOI

      10.2166/ws.2022.331

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Highly sensitive and homogeneous detection of unamplified RNA based on the light scattering properties of gold nanoparticle probes2022

    • Author(s)
      Satoh Hisashi、Nakajima Meri、Hirano Reiko、Nakaya Yuki
    • Journal Title

      Biosensors and Bioelectronics: X

      Volume: 12 Pages: 100249-100249

    • DOI

      10.1016/j.biosx.2022.100249

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Screening Antibiotic-Resistant <i>Escherichia coli</i> in Wastewater and River Water Using a Novel Simple Phenotypic Antibiotic-Susceptibility Testing Method2022

    • Author(s)
      Satoh Hisashi、Nagahashi Natsumi、Kikuchi Kai、Hirano Reiko
    • Journal Title

      ACS ES&T Water

      Volume: 2 Issue: 8 Pages: 1301-1308

    • DOI

      10.1021/acsestwater.1c00359

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of the simple analytical method for determination of arsenate(V) ion using fluorescence-labeled DNA and cerium oxide nanoparticles2022

    • Author(s)
      Koji Matsunaga, Hisashi Satoh, Reiko Hirano
    • Journal Title

      Water Supply

      Volume: 148 Issue: 5 Pages: 1-11

    • DOI

      10.2166/ws.2022.148

    • Related Report
      2022 Research-status Report 2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Simple enumeration of Escherichia coli concentrations in river water samples by measuring β-d-glucuronidase activities in a microplate reader2021

    • Author(s)
      Satoh Hisashi、Katayose Yutaka、Hirano Reiko
    • Journal Title

      Water Science and Technology

      Volume: 83 Issue: 6 Pages: 1399-1406

    • DOI

      10.2166/wst.2021.072

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Simple assay for colorimetric quantification of unamplified bacterial 16S rRNA in activated sludge using gold nanoprobes2021

    • Author(s)
      Nakajima Meri、Hirano Reiko、Okabe Satoshi、Satoh Hisashi
    • Journal Title

      Chemosphere

      Volume: 263 Pages: 128331-128331

    • DOI

      10.1016/j.chemosphere.2020.128331

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Development of a Paper-based Analytical Chip for the Detection of Bacterial 16S rRNA in Wastewater Samples2020

    • Author(s)
      NAKAJIMA Meri、ISHIDA Akihiko、TOKESHI Manabu、SATOH Hisashi
    • Journal Title

      BUNSEKI KAGAKU

      Volume: 69 Issue: 12 Pages: 715-722

    • DOI

      10.2116/bunsekikagaku.69.715

    • NAID

      130008021457

    • ISSN
      0525-1931
    • Year and Date
      2020-12-05
    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Presentation] A simple method for detecting fecal pollution in rivers by measuring β-D-glucuronidase activity2023

    • Author(s)
      Hisashi Satoh, Yuna Tanaka, Mohomed N. M. Shayan, Reiko Hirano, Yuki Nakaya
    • Organizer
      2023 Conference on Water & Wastewater Management, with special focus on Developing Countries
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Bacterial community structures and their regrowth in the urban rivers to which chlorinated reclaimed water is discharged from municipal wastewater treatment plants, 2023 Conference on Water & Wastewater Management2023

    • Author(s)
      Mohomed Shayan, Yuki Nakaya, Hisashi Satoh
    • Organizer
      2023 Conference on Water & Wastewater Management, with special focus on Developing Countries
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Prevalence of Antibiotic Resistant Genes (ARG) in Urban Rivers2022

    • Author(s)
      Mohomed Niyaz Mohomed Shayan, Takeshiro Onodera, Ryuta Miyajima, Yuya Akazawa, Hisashi Satoh
    • Organizer
      International conference on water convention of the Singapore International Water Week (SIWW, 2022)
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] 新規簡易微生物検出法による水道原水モニタリングの検討2022

    • Author(s)
      深田翔介,佐藤 久,平野麗子
    • Organizer
      第56回日本水環境学会年会
    • Related Report
      2021 Research-status Report
  • [Presentation] 金ナノ粒子プローブを用いた環境中の病原体の検出2022

    • Author(s)
      中島芽梨,平野麗子,中屋佑紀,佐藤 久
    • Organizer
      第56回日本水環境学会年会
    • Related Report
      2021 Research-status Report
  • [Presentation] 光導波路分光装置を用いた高感度簡易核酸モニタリング法の開発2021

    • Author(s)
      中島芽梨・中屋佑紀・佐藤久・平野麗子・高橋浩三
    • Organizer
      第 58 回環境工学研究フォーラム
    • Related Report
      2021 Research-status Report
  • [Presentation] Development of a simple analytical method for determination of arsenate using FAM-labeled DNA and cerium oxide nanoparticles2021

    • Author(s)
      Koji Matsunaga, Hisashi Satoh
    • Organizer
      Pacifichem 2021
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] Development of the simple analytical method for determination of Arsenate(V) using fluorescein-labeled DNA and cerium oxide nanoparticles2021

    • Author(s)
      Koji Matsunaga, Hisashi Satoh
    • Organizer
      International Conference on the challenges in Environmental Science and Engineering (CESE 2021)
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] Simple assay for spectroscopic quantification of 16S rRNA of ammonia oxidizing bacteria using DNA-modified gold nanoparticles2021

    • Author(s)
      Meri Nakajima, Hisashi Satoh
    • Organizer
      International Conference on the challenges in Environmental Science and Engineering (CESE 2021)
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] Legionella pneumophila と特異的に結合する新規DNAアプタマーの探索2021

    • Author(s)
      松永 光司、齋藤 慎吾、平野 麗子、佐藤 久
    • Organizer
      第55回日本水環境学会年会
    • Related Report
      2020 Research-status Report
  • [Presentation] 光導波路分光装置を用いた下水処理プロセスの微生物モニタリング2021

    • Author(s)
      中島芽梨、平野麗子、高橋浩三、佐藤久
    • Organizer
      第55回日本水環境学会年会
    • Related Report
      2020 Research-status Report
  • [Presentation] 新規簡易大腸菌分析技術を用いた都市河川の糞便汚染源の解明2021

    • Author(s)
      宮島龍太、赤澤優弥、小野寺岳史郎、佐藤久
    • Organizer
      第55回日本水環境学会年会
    • Related Report
      2020 Research-status Report
  • [Presentation] 都市河川における病原遺伝子および薬剤耐性遺伝子の分布2021

    • Author(s)
      小野寺岳史郎、岩﨑隼、平野麗子、佐藤久
    • Organizer
      第55回日本水環境学会年会
    • Related Report
      2020 Research-status Report
  • [Remarks] 佐藤久研究グループWebsite

    • URL

      https://www.eng.hokudai.ac.jp/labo/aqua/contents/HisashiSatoh/index-HisashiSatoh.html

    • Related Report
      2023 Annual Research Report
  • [Remarks] 北大 佐藤久研究グループ

    • URL

      https://www.eng.hokudai.ac.jp/labo/aqua/contents/HisashiSatoh/index-HisashiSatoh.html

    • Related Report
      2022 Research-status Report 2021 Research-status Report 2020 Research-status Report

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Published: 2020-10-29   Modified: 2025-01-30  

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