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Development of a bioactive ink library for functional three-dimensional tissue bioprinting

Research Project

Project/Area Number 20KK0112
Research Category

Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 27:Chemical engineering and related fields
Research InstitutionOsaka University

Principal Investigator

SAKAI SHINJI  大阪大学, 大学院基礎工学研究科, 教授 (20359938)

Co-Investigator(Kenkyū-buntansha) 小嶋 勝  大阪大学, 大学院基礎工学研究科, 准教授 (00533647)
中畑 雅樹  大阪大学, 大学院理学研究科, 助教 (40755641)
Project Period (FY) 2020-10-27 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2023: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2022: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2021: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2020: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsバイオプリンティング / 組織工学 / 再生医療 / 3Dプリント / ゲル / バイオインク / 3Dプリンティング
Outline of Research at the Start

3Dプリンティングは、複雑な内部構造を持つ構造体の造形を可能とする。特に、インクに細胞を含ませて用いるバイオプリンティングは、現状はまだ不可能なものの、従来技術では造形できなかった機能的な組織や臓器の代替物の作製を実現する技術として期待されている。そして、その実現には、プリンタの進化とともにインクの進化が不可欠である。本研究では、フランスの研究グループと共同研究にて、インクの進化に不可欠な新たな生物活性物質を天然資源の中から見出し、それに必要な機能を付与することで、それらの材料からなるインクライブラリを構築することに取り組む。

Outline of Final Research Achievements

3D printing enables the modeling of structures with complex internal structures. In particular, bioprinting, which uses cells in ink, is expected to be a technology that will enable the fabrication of functional tissue and organ substitutes that cannot be modeled with conventional technologies. In addition to the evolution of printers, the evolution of inks is indispensable to realize this technology. In this study, in collaboration with a research group in France, we investigated the possibility of finding new bioactive substances from natural resources, giving them the necessary functions, and obtaining new ink materials. As a result of this study, several polysaccharides were discovered, including polyglucuronic acid, and major results were obtained that led to patent applications and the publication of scientific papers.

Academic Significance and Societal Importance of the Research Achievements

3Dプリンティングは、複雑な内部構造を持つ構造体の造形を可能とする。特に、インクに細胞を含ませて用いるバイオプリンティングは、現状はまだ不可能なものの、従来技術では造形できなかった機能的な組織や臓器の代替物の作製を実現する技術として期待されている。特に、社会的には、この技術の成熟は移植待機患者の命を救うこと、医療コストの削減や治療の質の向上にも寄与すると期待されている。本研究の成果として有望なインク材料を獲得できたことは、バイオプリンティングに重要な前進をもたらすものと期待される。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (41 results)

All 2024 2023 2022 2021 Other

All Int'l Joint Research (3 results) Journal Article (15 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 15 results,  Open Access: 9 results) Presentation (21 results) (of which Int'l Joint Research: 6 results,  Invited: 4 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 2 results)

  • [Int'l Joint Research] Unversity of Indonesia(インドネシア)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] University of Clermont Auvergne/University of Picardy Jules Verne(フランス)

    • Related Report
      2022 Research-status Report
  • [Int'l Joint Research] University of Clermont Auvergne/University of Picardy Jules Verne(フランス)

    • Related Report
      2020 Research-status Report
  • [Journal Article] Enzymatically gellable chitosan inks with enhanced printability by chitosan nanofibers for 3D printing of wound dressings2024

    • Author(s)
      Sakai Shinji、Yamamoto Shota、Hirami Ryo、Hidaka Mitsuyuki、Chamara Manoj Lakmal Elvitigala Kelum
    • Journal Title

      European Polymer Journal

      Volume: 210 Pages: 112960-112960

    • DOI

      10.1016/j.eurpolymj.2024.112960

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of Chitosan Hydrogels Obtained through Phenol and Tripolyphosphate Anionic Crosslinking2024

    • Author(s)
      Mitsuyuki Hidaka, Masaru Kojima, Shinji Sakai, Cedric Delattre
    • Journal Title

      Polymers

      Volume: 16 Issue: 9 Pages: 1274-1274

    • DOI

      10.3390/polym16091274

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Tuning the crosslinking and degradation of hyaluronic acid/gelatin hydrogels using hydrogen peroxide for muscle cell sheet fabrication2023

    • Author(s)
      Elvitigala Kelum Chamara Manoj Lakmal、Mubarok Wildan、Sakai Shinji
    • Journal Title

      Soft Matter

      Volume: 19 Issue: 31 Pages: 5880-5887

    • DOI

      10.1039/d3sm00560g

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Visible light photocrosslinking of sugar beet pectin for 3D bioprinting applications2023

    • Author(s)
      Mubarok Wildan、Elvitigala Kelum Chamara Manoj Lakmal、Kotani Takashi、Sakai Shinji
    • Journal Title

      Carbohydrate Polymers

      Volume: 316 Pages: 121026-121026

    • DOI

      10.1016/j.carbpol.2023.121026

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Horseradish Peroxidase-Mediated Bioprinting via Bioink Gelation by Alternately Extruded Support Material2023

    • Author(s)
      Kotani Takashi、Mubarok Wildan、Hananouchi Takehito、Sakai Shinji
    • Journal Title

      ACS Biomaterials Science & Engineering

      Volume: 9 Issue: 10 Pages: 5804-5812

    • DOI

      10.1021/acsbiomaterials.3c00996

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fabrication of cell-laden microbeads and microcapsules composed of bacterial polyglucuronic acid2023

    • Author(s)
      Goto Ryota、Nakahata Masaki、Delattre C?dric、Petit Emmanuel、El Boutachfaiti Redouan、Sakai Shinji
    • Journal Title

      International Journal of Biological Macromolecules

      Volume: 244 Pages: 125481-125481

    • DOI

      10.1016/j.ijbiomac.2023.125481

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Development of non-adherent cell-enclosing domes with enzymatically cross-linked hydrogel shell2023

    • Author(s)
      Kazama Ryotaro、Sato Ryuta、Fujiwara Hiroyuki、Qu Yanfei、Nakahata Masaki、Kojima Masaru、Fujita Satoshi、Sakai Shinji
    • Journal Title

      Biofabrication

      Volume: 15 Issue: 1 Pages: 015002-015002

    • DOI

      10.1088/1758-5090/ac95ce

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Cell Dome as an Evaluation Platform for Organized HepG2 Cells2023

    • Author(s)
      Kazama Ryotaro、Fujita Satoshi、Sakai Shinji
    • Journal Title

      Cells

      Volume: 12 Issue: 1 Pages: 69-69

    • DOI

      10.3390/cells12010069

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] One-Step FRESH Bioprinting of Low-Viscosity Silk Fibroin Inks2022

    • Author(s)
      Sakai Shinji、Morita Takahiro
    • Journal Title

      ACS Biomaterials Science and Engineering

      Volume: 8 Issue: 6 Pages: 2589-2597

    • DOI

      10.1021/acsbiomaterials.2c00269

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Tuning Myogenesis by Controlling Gelatin Hydrogel Properties through Hydrogen Peroxide-Mediated Cross-Linking and Degradation2022

    • Author(s)
      Mubarok Wildan、Elvitigala Kelum Chamara Manoj Lakmal、Sakai Shinji
    • Journal Title

      Gels

      Volume: 8 Issue: 6 Pages: 387-387

    • DOI

      10.3390/gels8060387

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Human Umbilical Vein Endothelial Cells Form a Network on a Hyaluronic Acid/Gelatin Composite Hydrogel Moderately Crosslinked and Degraded by Hydrogen Peroxide2022

    • Author(s)
      Elvitigala Kelum Chamara Manoj Lakmal、Mubarok Wildan、Sakai Shinji
    • Journal Title

      Polymers

      Volume: 14 Issue: 22 Pages: 5034-5034

    • DOI

      10.3390/polym14225034

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Phenol-Grafted Alginate Sulfate Hydrogel as an Injectable FGF-2 Carrier2022

    • Author(s)
      Goto Ryota、Nakahata Masaki、Sakai Shinji
    • Journal Title

      Gels

      Volume: 8 Issue: 12 Pages: 818-818

    • DOI

      10.3390/gels8120818

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of phenol-grafted polyglucuronic acid and its application to extrusion-based bioprinting inks2022

    • Author(s)
      Sakai Shinji、Kotani Takashi、Harada Ryohei、Goto Ryota、Morita Takahiro、Bouissil Soukaina、Dubessay Pascal、Pierre Guillaume、Michaud Philippe、El Boutachfaiti Redouan、Nakahata Masaki、Kojima Masaru、Petit Emmanuel、Delattre C?dric
    • Journal Title

      Carbohydrate Polymers

      Volume: 277 Pages: 118820-118820

    • DOI

      10.1016/j.carbpol.2021.118820

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Modulation of Cell-Cycle Progression by Hydrogen Peroxide-Mediated Cross-Linking and Degradation of Cell-Adhesive Hydrogels2022

    • Author(s)
      Mubarok Wildan、Elvitigala Kelum Chamara Manoj Lakmal、Nakahata Masaki、Kojima Masaru、Sakai Shinji
    • Journal Title

      Cells

      Volume: 11 Issue: 5 Pages: 881-881

    • DOI

      10.3390/cells11050881

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Freeform 3D Bioprinting Involving Ink Gelation by Cascade Reaction of Oxidase and Peroxidase: A Feasibility Study Using Hyaluronic Acid-Based Ink2021

    • Author(s)
      Sakai Shinji、Harada Ryohei、Kotani Takashi
    • Journal Title

      Biomolecules

      Volume: 11 Issue: 12 Pages: 1908-1908

    • DOI

      10.3390/biom11121908

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 3D bioprinting of sugar beet pectin obtained through horseradish peroxidase-catalyzed crosslinking2024

    • Author(s)
      平見 凌、堀口 一樹、小嶋 勝、境 慎司
    • Organizer
      化学工学会第89年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 酵素反応で得られるゲルとそれを使った3Dバイオプリント2024

    • Author(s)
      境 慎司
    • Organizer
      2023年度第7回広島大学大学院医系科学研究科・大学病院合同FD
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] The Effect of Oxidative Degradation of Phenolated Hyaluronic Acid on HUVEC Network Formation2023

    • Author(s)
      Elvitigala Kelum Chamara Manoj Lakmal, Wildan Mubarok, 堀口一機、小嶋 勝、境 慎司
    • Organizer
      化学工学会第54回秋季大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Human Umbilical Vein Endothelial Cells Behaviour on Hyaluronic Acid-based Hydrogel Obtained via Hydrogen Peroxide-Mediated Crosslinking and Degradation2023

    • Author(s)
      Elvitigala Kelum Chamara Manoj Lakmal, Wildan Mubarok, Horiguchi Ikki、Kojima Masaru、Sakai Shinji
    • Organizer
      8th International Symposium on Biomedical engineering 2023
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The Impact of Tyramine-Modified Hyaluronic Acid on Human Umbilical Vein Endothelial Cell Network Formation2023

    • Author(s)
      Elvitigala Kelum Chamara Manoj Lakmal, 堀口一機、小嶋 勝、境 慎司
    • Organizer
      化学工学会第89年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] バイオインク混合のための3Dプリンタ用バブルマイクロミキサの開発2023

    • Author(s)
      日髙光将, 小嶋勝, 堀口一樹, 境慎司
    • Organizer
      化学工学会第89年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] インクと交互に吐出された支持材料からの物質供給によるゲル化を利用した3Dバイオプリンティング手法の開発2023

    • Author(s)
      粉谷 聖、堀口 一樹、小嶋 勝、境 慎司
    • Organizer
      化学工学会第89年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 3D Bioprinting Technologies Based on Enzymatic Hydrogelation2023

    • Author(s)
      Shinji Sakai
    • Organizer
      8th International Symposium on Biomedical engineering 2023
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ポリグルクロン酸の細胞包括マイクロ粒子への応用2023

    • Author(s)
      後藤良太, Cdric Delattre, 堀口一樹, 小嶋勝, 境慎司
    • Organizer
      化学工学会第88年会
    • Related Report
      2022 Research-status Report
  • [Presentation] 高度な細胞培養足場構築を目的としたマルチマテリアル3Dバイオプリンティング用単一ノズルの開発2022

    • Author(s)
      日髙光将, Zhang Colin, 小嶋勝, 中畑雅樹, 堀口 一樹, 岡野 泰典, 境慎司
    • Organizer
      化学工学会第53回秋季大会
    • Related Report
      2022 Research-status Report
  • [Presentation] Human umbilical vein endothelial cell behavior on Gelatin-Ph/HA-Ph composite hydrogel obtained via2022

    • Author(s)
      Elvitigala Kelum Chamara Manoj Lakmal, Wildan Mubarok, 堀口一機, 小嶋 勝, 境 慎司
    • Organizer
      第74回日本生物工学会大会
    • Related Report
      2022 Research-status Report
  • [Presentation] 新規多糖の骨組織工学への応用2022

    • Author(s)
      後藤良太, Cedric Delattre, 堀口 一樹, 小嶋 勝, 境 慎司
    • Organizer
      化学工学会第53回秋季大会
    • Related Report
      2022 Research-status Report
  • [Presentation] 支持材料とインクの交互積層による可視光架橋3Dバイオプリンティング手法の開発2022

    • Author(s)
      粉谷 聖, 堀口 一樹, 小嶋 勝, 境 慎司
    • Organizer
      化学工学会第53回秋季大会
    • Related Report
      2022 Research-status Report
  • [Presentation] 酵素を使った3Dバイオプリンティング2021

    • Author(s)
      境 慎司
    • Organizer
      日本バイオマテリアル学会 2021年度 九州ブロック学術講演会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] 酵素を使った医療・ヘルスケアのためのものづくり2021

    • Author(s)
      境 慎司
    • Organizer
      第15回 メタボロームシンポジウム
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] 3Dバイオプリンティングによる水性二相系インクを用いたヒドロゲルファイバーの作製2021

    • Author(s)
      森田 崇裕・中畑 雅樹・小嶋 勝・境 慎司
    • Organizer
      化学工学会第 52 回秋季大会
    • Related Report
      2021 Research-status Report
  • [Presentation] マイクロ流体デバイスを用いた気泡を孔源とする多孔質ヒドロゲル構造体の作製2021

    • Author(s)
      原田 涼平・ 中畑 雅樹・ 小嶋 勝・ 境 慎司
    • Organizer
      化学工学会第 52 回秋季大会
    • Related Report
      2021 Research-status Report
  • [Presentation] Biocompatible solar hydrogelation mediated by resorcinol-formaldehyde resins and horseracdih peroxidase2021

    • Author(s)
      KITATANI Yuki, SHIRAISHI Yasuhiro, NAKAHATA Masaki, KOJIMA Masaru, HIRAI Takayuki, SAKAI Shinji
    • Organizer
      Materials Research Meeting 2021
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] Photocurable chitosan ink with rapid gelation for extrusion-based/vat polymerization-based printings2021

    • Author(s)
      Hidaka Mitsyuki, Kojim Masaru, Nakahata Masaki and Sakai Shinji
    • Organizer
      Materials Research Meeting 2021
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] Influence of Hydrogen Peroxide-Mediated Crosslinking and Polymer Degradation to Cells Adhesion2021

    • Author(s)
      Wildan Mubarok, Masaki Nakahata, Masaru Kojima, Shinji Sakai
    • Organizer
      第8回アジアバイオマテリアル学会
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] Modulation of Cells Adherence by Hydrogen Peroxide-Mediated Crosslinking and Polymer Degradation2021

    • Author(s)
      Wildan Mubarok, Masaki Nakahata, Masaru Kojima, Shinji Sakai
    • Organizer
      The 26th Symposium of Young Asian Biological Engineers’ Community
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Patent(Industrial Property Rights)] POLYMERIC COMPOUND OF GLUCURONIC ACID WITH PHENOLIC GROUPS, GEL-FORMING COMPOSITION COMPRISING SUCH A COMPOUND AND METHOD FOR PRODUCING THE SAME2023

    • Inventor(s)
      C DELATTRE, S SAKAI et al
    • Industrial Property Rights Holder
      クレルモン・オーベルニュ大学, 大阪大学 他
    • Industrial Property Rights Type
      特許
    • Filing Date
      2023
    • Related Report
      2023 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] POLYMERIC COMPOUND OF GLUCURONIC ACID WITH PHENOLIC GROUPS, GELFORMING COMPOSITION COMPRISING SUCH A COMPOUND AND METHOD FOR PRODUCING THE SAME2021

    • Inventor(s)
      DELATTRE C, SAKAI S, et al
    • Industrial Property Rights Holder
      DELATTRE C, SAKAI S, et al
    • Industrial Property Rights Type
      特許
    • Filing Date
      2021
    • Related Report
      2021 Research-status Report
    • Overseas

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Published: 2020-10-29   Modified: 2025-01-30  

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