Genomic epidemiological survey on cattle tick-borne diseases in Philippines and establishment of practical diagnosis

• Project/Area Number: 20KK0154
• Research Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 42:Veterinary medical science, animal science, and related fields
• Research Institution: Kagoshima University
• Principal Investigator: 田仲 哲也 鹿児島大学, 農水産獣医学域獣医学系, 教授 (00322842)
• Co-Investigator(Kenkyū-buntansha): 草木迫 浩大 北里大学, 獣医学部, 助教 (10838220) ; 二神 泰基 鹿児島大学, 農水産獣医学域農学系, 准教授 (60512027) ; 山崎 渉 京都大学, 東南アジア地域研究研究所, 教授 (70393262)
• Project Period (FY): 2020-10-27 – 2025-03-31
• Project Status: Granted (Fiscal Year 2023)
• Budget Amount: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2023: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2022: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2021: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2020: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
• Keywords: 牛マダニ媒介性感染症 / マダニ / ピロプラズマ症 / PCR / LAMP / RPA / ゲノム / ジェノタイプ

Outline of Research at the Start

マダニ媒介性感染症は、人や家畜に致死的な症状を引き起こし、地球規模で畜産業の脅威となっている。マダニ媒介性感染症の制御には、流行実態をグローバル(空間的)な視点により解明し、その地で現在流行しているマダニ媒介性感染症に適した「現地即応型」な診断対策を講じる必要がある。
本研究では、我が国と畜産・農産品の輸入貿易が盛んなフィリピンに着目した。当国における遺伝的に多様なマダニ媒介性病原体の集団を対象とし、ゲノム疫学的手法に基づくマダニ媒介性感染症の流行実態の解明と、ゲノム情報に立脚した現地即応型のマダニ媒介性感染症における診断法の構築を目指す。 本成果は畜産業向上のための「国際貢献」につながる。

Outline of Annual Research Achievements

マダニ媒介性感染症は、人や家畜に致死的な症状を引き起こし、地球規模で畜産業の脅威となっている。マダニ媒介性感染症の制御には、流行実態をグローバル(空間的)な視点により解明し、その地で現在流行しているマダニ媒介性感染症に適した「現地即応型」な診断対策を講じる必要がある。本研究では、我が国と畜産・農産品の輸入貿易が盛んなフィリピンに着目した。当国における遺伝的に多様なマダニ媒介性病原体の集団を対象とし、ゲノム疫学的手法に基づくマダニ媒介性感染症の流行実態の解明と、ゲノム情報に立脚した現地即応型のマダニ媒介性感染症における診断法の構築を目指す。本成果は畜産業向上のための国際貢献」につながる。本研究はフィリピンにおける牛マダニ媒介性感染症の流行実態を明らかにし、フィリピンの診断対策の向上に資することを最終目標とした。
【現地フィールドワーク 主担当：田仲・Galay】
DNAサンプル解析：2023年にフィリピン北部で採取した牛の血液DNAサンプルを用いて、牛ピロプラズマ症の遺伝子診断・解析を行った。
【国内ラボワーク 主担当：田仲・草木迫】
遺伝子診断法の構築：牛のピロプラズマ症について、現地で利用し易いLAMPやRPA解析用のプライマーを考案し、LAMP法やRPA法を確立した。

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

2023年8月に共同研究者であるフィリピン大学からGalay博士が来日したことと、2024年3月研究代表者である田仲がフィリピン大学を訪問し、本研究に関する研究打ち合わせを行うことができ、お互いの連携が確認できたため。

Strategy for Future Research Activity

【現地フィールドワーク 主担当：田仲・草木迫・Galay・Divina・Pilapil-Amante・Talactac】
・フィリピンにおける牛マダニ媒介性感染症の疫学調査：広範囲の疫学調査と主要流行マダニ媒介性病原体の特定
① 調査地域選定：フィリピン北部、中部、南部を調査地域として選定する。② サンプル採集：各地域において、牛・水牛農家を選定し、血液サンプルを採集し、PCRによる簡易診断を実施する。簡易診断結果に基づいて本格的調査の場所を決定する。③ 遺伝子診断：LAMPによる遺伝子診断を実施する。④ 流行株の特定と核酸の抽出：上記DNA診断結果に基づいて、主要流行株を特定する。次に、特定した牛・水牛より大量血液サンプルを採取後、全DNAを抽出し、日本に搬入する。
【国内ラボワーク 主担当：草木迫・二神・田仲】
・主要流行株の全ゲノム解読とデータベースの構築①次世代シークエンサによる各種病原体の全ゲノム解読：主要流行株の全ゲノムについて、次世代シークエンサーMiSeqやナノポアシークエンサーMinIONを用いて解読する。② 遺伝的多型の推定：ゲノム全体の遺伝的多型を最も良く反映する可変領域を全ゲノムの解析結果から特定する。③ データベースの解析・構築：ナノポアシークエンサーと次世代シークエンサーMiSeqで得られた結果から、主要流行株のゲノムデータベースを構築する。

Research Products

• [Int'l Joint Research] フィリピン大学・獣医学部/カヴィテ州立大学・獣医生命科学部(フィリピン)
• [Int'l Joint Research] フィリピン大学・獣医学部/カヴィテ州立大学・獣医生命科学部(フィリピン)
• [Int'l Joint Research] フィリピン大学・獣医学部/カヴィテ州立大学・獣医生命科学部(フィリピン)
• [Int'l Joint Research] フィリピン大学・獣医学部/カビテ州立大学・獣医生命科学部(フィリピン)
• [Journal Article] First record of Alectorobius coniceps (Ixodoidea: Argasidae) and Dermacentor sp. (Ixodoidea: Ixodidae) in Pakistan (2024)
  • Author(s): Ali Abid、Khan Mehran、Ullah Zafar、Numan Muhammad、Tsai Kun-Hsien、Alouffi Abdulaziz、Almutairi Mashal M.、Tanaka Tetsuya
  • Journal Title: Frontiers in Veterinary Science Volume: 10 Pages: 1326734-1326734
  • DOI: 10.3389/fvets.2023.1326734
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular phylogenetic analysis of Echinococcus multilocularis from horses raised in Canada or Japan, using mitochondrial cytochrome b gene?targeted PCR (2024)
  • Author(s): Hifumi Tatsuro、Tanaka Tetsuya、Suzu Ichinosuke、Sato Miho、Akioka Kohei、Fujimata Chiaki、Shinkai Ryohei、Maeda Tsutomu、Kusakisako Kodai、Ikadai Hiromi、Miyoshi Noriaki
  • Journal Title: Food and Waterborne Parasitology Volume: 34 Pages: e00219-e00219
  • DOI: 10.1016/j.fawpar.2024.e00219
  • Peer Reviewed / Open Access
• [Journal Article] Molecular detection and characterization of Theileria annulata, Babesia bovis, and Babesia bigemina infecting cattle and buffalo in southern Egypt (2024)
  • Author(s): Mahmoud Hassan Y.A.H.、Rady Abdelrahman A.、Tanaka Tetsuya
  • Journal Title: Parasite Epidemiology and Control Volume: 25 Pages: e00340-e00340
  • DOI: 10.1016/j.parepi.2024.e00340
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular detection and diversity of tick-borne rickettsial pathogens in ticks collected from camel (Camelus dromedarius) in Upper Egypt (2024)
  • Author(s): Soliman Ahmed M.、Mahmoud Hassan Y.A.H.、Amer Moaz M.、Hifumi Tatsuro、Tanaka Tetsuya
  • Journal Title: Acta Tropica Volume: 253 Pages: 107172-107172
  • DOI: 10.1016/j.actatropica.2024.107172
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] First Report of Rickettsia conorii in Hyalomma kumari Ticks (2023)
  • Author(s): Ullah Shafi、Alouffi Abdulaziz、Almutairi Mashal M.、Islam Nabila、Rehman Gauhar、Ul Islam Zia、Ahmed Haroon、J?nior Itabajara da Silva Vaz、Labruna Marcelo B.、Tanaka Tetsuya、Ali Abid
  • Journal Title: Animals Volume: 13 Issue: 9 Pages: 1488-1488
  • DOI: 10.3390/ani13091488
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular detection and characterization of Anaplasma marginale infecting cattle, buffalo, and camel populations in southern Egypt (2023)
  • Author(s): Mahmoud Hassan Y. A. H.、Ali Alsagher O.、Tanaka Tetsuya
  • Journal Title: Frontiers in Veterinary Science Volume: 10 Pages: 1169323-1169323
  • DOI: 10.3389/fvets.2023.1169323
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular detection of Bartonella and Borrelia in pet dogs in Metro Manila and Laguna, Philippines (2023)
  • Author(s): Lee Chae Eun、Ikeda Jeong Hee、Manongdo Mikaella Andrea M.、Romerosa Dan Rica T.、Sandalo-De Ramos Kristina Andrea C.、Tanaka Tetsuya、Galay Remil L.
  • Journal Title: Veterinary World Volume: 16 Pages: 1546-1551
  • DOI: 10.14202/vetworld.2023.1546-1551
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular detection of Coxiella spp. in ticks (Ixodidae and Argasidae) infesting domestic and wild animals: with notes on the epidemiology of tick-borne Coxiella burnetii in Asia (2023)
  • Author(s): Ali Abid、Obaid Muhammad Kashif、Almutairi Mashal M.、Alouffi Abdulaziz、Numan Muhammad、Ullah Shafi、Rehman Gauhar、Islam Zia Ul、Khan Sher Bahadar、Tanaka Tetsuya
  • Journal Title: Frontiers in Microbiology Volume: 14 Pages: 1229950-1229950
  • DOI: 10.3389/fmicb.2023.1229950
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Association of SFG Rickettsia massiliae and Candidatus Rickettsia shennongii with Different Hard Ticks Infesting Livestock Hosts (2023)
  • Author(s): Shehla Shehla、Ullah Farman、Alouffi Abdulaziz、Almutairi Mashal M.、Khan Zaibullah、Tanaka Tetsuya、Labruna Marcelo B.、Tsai Kun-Hsien、Ali Abid
  • Journal Title: Pathogens Volume: 12 Issue: 9 Pages: 1080-1080
  • DOI: 10.3390/pathogens12091080
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] First report on tick-borne pathogens detected in ticks infesting stray dogs near butcher shops (2023)
  • Author(s): Ali Abid、Ullah Shafi、Numan Muhammad、Almutairi Mashal M.、Alouffi Abdulaziz、Tanaka Tetsuya
  • Journal Title: Frontiers in Veterinary Science Volume: 10 Pages: 1256871-126871
  • DOI: 10.3389/fvets.2023.1246871
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] First report on detection of Hepatozoon ayorgbor in Rhipicephalus haemaphysaloides and Hepatozoon colubri in Haemaphysalis sulcata and Hyalomma anatolicum: risks of spillover of Hepatozoon spp. from wildlife to domestic animals (2023)
  • Author(s): Tila Hadia、Khan Mehran、Almutairi Mashal M.、Alouffi Abdulaziz、Ahmed Haroon、Tanaka Tetsuya、Tsai Kun-Hsien、Ali Abid
  • Journal Title: Frontiers in Veterinary Science Volume: 10 Pages: 1255482-1255482
  • DOI: 10.3389/fvets.2023.1255482
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular Detection of Rickettsia hoogstraalii in Hyalomma anatolicum and Haemaphysalis sulcata: Updated Knowledge on the Epidemiology of Tick-Borne Rickettsia hoogstraalii (2023)
  • Author(s): Aneela Aneela、Almutairi Mashal M.、Alouffi Abdulaziz、Ahmed Haroon、Tanaka Tetsuya、da Silva Vaz Itabajara、Chang Shun-Chung、Chen Chien-Chin、Ali Abid
  • Journal Title: Veterinary Sciences Volume: 10 Issue: 10 Pages: 605-605
  • DOI: 10.3390/vetsci10100605
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Argas persicus and Carios vespertilionis Ticks Infesting Ducks, Domestic Fowls and Bats in Pakistan: First Report on Molecular Survey and Phylogenetic Position of Borrelia anserina (2023)
  • Author(s): Zahid Hafsa、Alouffi Abdulaziz、Almutairi Mashal M.、Ateeq Muhammad、Tanaka Tetsuya、Chang Shun-Chung、Chen Chien-Chin、Ali Abid
  • Journal Title: Veterinary Sciences Volume: 10 Issue: 10 Pages: 628-628
  • DOI: 10.3390/vetsci10100628
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] First Detection of Theileria sinensis-like and Anaplasma capra in Ixodes kashmiricus: With Notes on cox1-Based Phylogenetic Position and New Locality Records (2023)
  • Author(s): Numan Muhammad、Alouffi Abdulaziz、Almutairi Mashal M.、Tanaka Tetsuya、Ahmed Haroon、Akbar Haroon、Rashid Muhammad Imran、Tsai Kun-Hsien、Ali Abid
  • Journal Title: Animals Volume: 13 Issue: 20 Pages: 3232-3232
  • DOI: 10.3390/ani13203232
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular Survey of Rickettsia raoultii in Ticks Infesting Livestock from Pakistan with Notes on Pathogen Distribution in Palearctic and Oriental Regions (2023)
  • Author(s): Shehla Shehla、Almutairi Mashal M.、Alouffi Abdulaziz、Tanaka Tetsuya、Chang Shun-Chung、Chen Chien-Chin、Ali Abid
  • Journal Title: Veterinary Sciences Volume: 10 Issue: 11 Pages: 636-636
  • DOI: 10.3390/vetsci10110636
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] First Molecular-Based Confirmation of Dermacentor marginatus and Associated Rickettsia raoultii and Anaplasma marginale in the Hindu Kush Mountain Range (2023)
  • Author(s): Ahmad Iftikhar、Ullah Shafi、Alouffi Abdulaziz、Almutairi Mashal M.、Numan Muhammad、Tanaka Tetsuya、Chang Shun-Chung、Chen Chien-Chin、Ali Abid
  • Journal Title: Animals Volume: 13 Issue: 23 Pages: 3686-3686
  • DOI: 10.3390/ani13233686
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular evidence of Borrelia theileri and closely related Borrelia spp. in hard ticks infesting domestic animals (2023)
  • Author(s): Khan Mehran、Almutairi Mashal M.、Alouffi Abdulaziz、Tanaka Tetsuya、Chang Shun-Chung、Chen Chien-Chin、Ali Abid
  • Journal Title: Frontiers in Veterinary Science Volume: 10 Pages: 1297928-1297928
  • DOI: 10.3389/fvets.2023.1297928
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] First report of spotted fever group Rickettsia aeschlimannii in Hyalomma turanicum, Haemaphysalis bispinosa, and Haemaphysalis montgomeryi infesting domestic animals: updates on the epidemiology of tick-borne Rickettsia aeschlimannii (2023)
  • Author(s): Majid Abdul、Almutairi Mashal M.、Alouffi Abdulaziz、Tanaka Tetsuya、Yen Tsai-Ying、Tsai Kun-Hsien、Ali Abid
  • Journal Title: Frontiers in Microbiology Volume: 14 Pages: 1283814-1283814
  • DOI: 10.3389/fmicb.2023.1283814
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Phylogenetic Position of Haemaphysalis kashmirensis and Haemaphysalis cornupunctata, with Notes on Rickettsia spp. (2023)
  • Author(s): Khan, S. M., Khan, M., Alouffi, A., Almutairi, M. M., Numan, M., Ullah, S., Obaid, M. K., Islam, Z. U., Ahmed, H., Tanaka, T. and Ali, A.
  • Journal Title: Genes Volume: 14 (2) Issue: 2 Pages: 360-360
  • DOI: 10.3390/genes14020360
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Clinical and pathological findings in fatal cases of severe fever with thrombocytopenia syndrome with high viremia in cats (2023)
  • Author(s): Matsuu, A., Hatai, H., Hifumi, T., Hamakubo, E., Take, M., Tanaka, T., Momoi, Y., Endo, Y., Koyoshi, A., Kamikubo, Y., Kamatsuki, R., Kurusu, N., Tabata, H., Matsuyama, H., Yanaidani, M., Kawabata, M. and Kawabata, T.
  • Journal Title: Top. Companion Anim. Med. Volume: 52 Pages: 100756-100756
  • DOI: 10.1016/j.tcam.2022.100756
  • Peer Reviewed
• [Journal Article] Rapid detection of alveolar echinococcosis in hepatic nodules of horses by recombinase polymerase amplification assay . (2023)
  • Author(s): Hifumi, T., Tanaka, T., Sato, M., Akioka, K., Fujimata, C. and Miyoshi, N.
  • Journal Title: Vet. Anim. Sci. Volume: 20 Pages: 100291-100291
  • DOI: 10.1016/j.vas.2023.100291
  • Peer Reviewed / Open Access
• [Journal Article] Molecular survey and genetic characterization of Anaplasma marginale in ticks collected from livestock hosts in Pakistan (2022)
  • Author(s): Khan, Z., Shehla, S., Alouffi, A., Kashif Obaid, M., Zeb Khan, A., Almutairi, MM., Numan, M., Aiman, O., Alam, S., Ullah, S., Zaman Safi, S., Tanaka, T. and Ali, A.
  • Journal Title: Animals (Basel) Volume: 12 (13) Issue: 13 Pages: 1708-1708
  • DOI: 10.3390/ani12131708
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Spatio-temporal patterns of ticks and molecular survey of Anaplasma marginale, with notes on their phylogeny (2022)
  • Author(s): Alam, S., Khan, M., Alouffi, A., Almutairi, M. M., Ullah, S., Numan, M., Islam, N., Khan, Z., Aiman, O., Zaman Safi, S., Tanaka, T. and Ali, A.
  • Journal Title: Microorganisms Volume: 10 (8) Issue: 8 Pages: 1663-1663
  • DOI: 10.3390/microorganisms10081663
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular characterization and assessment of risk factors associated with Theileria annulate infection (2022)
  • Author(s): Ullah, K., Numan, M., Alouffi, A., Almutairi, M. M., Zahid, H., Khan, M., Islam, Z. U., Kamil, A., Safi, S. Z., Ahmed, H., Tanaka, T. and Ali, A.
  • Journal Title: Microorganisms Volume: 10 ( 8 ) Issue: 8 Pages: 1614-1614
  • DOI: 10.3390/microorganisms10081614
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Polymorphism in the immunogenic sequences of Rhipicephalus microplus clade c (2022)
  • Author(s): Zeb, I., Almutairi, M. M., Alouffi, A., Islam, N., Parizi, L. F., Safi, S. Z., Tanaka, T., da Silva Vaz, I Jr. and Ali, A.
  • Journal Title: Vaccines Volume: 10 ( 11 ) Issue: 11 Pages: 1909-1909
  • DOI: 10.3390/vaccines10111909
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Description of male, redescription of female, host record, and phylogenetic position of Haemaphysalis danieli (2022)
  • Author(s): Ahmad, I., Ullah, S., Alouffi, A., Almutairi, M. M., Khan, M., Numan, M., Safi, S. Z., Chitimia-Dobler, L., Tanaka, T. and Ali, A.
  • Journal Title: Pathogens Volume: 11 (12) Issue: 12 Pages: 1495-1495
  • DOI: 10.3390/pathogens11121495
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular Survey and Spatial Distribution of Rickettsia spp. in Ticks Infesting Free-Ranging Wild Animals in Pakistan (2017-2021) (2022)
  • Author(s): Ali, A., Shehla, S., Zahid, H., Ullah, F., Zeb, I., Ahmed, H., da Silva Vaz, I Jr. and Tanaka, T.
  • Journal Title: Pathogens Volume: 11 Issue: 2 Pages: 162-162
  • DOI: 10.3390/pathogens11020162
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Rapid Spread of Severe Fever with Thrombocytopenia Syndrome Virus by Parthenogenetic Asian Longhorned Ticks (2022)
  • Author(s): Zhang, X., Zhao, C., Cheng, C., Zhang, G., Yu, T., Lawrence, K., Li, H., Sun, J., Yang, Z., Ye, L., Chu, H., Wang, Y., Han, X., Jia, Y., Fan, S., Kanuka, H., Tanaka, T., Jenkins, C., Gedye, K., Chandra, S., Price, D. C., Liu, Q., Choi, Y. K., Zhan, X., Zhang, Z. and Zheng, A.
  • Journal Title: Emerging Infectious Diseases Volume: 28 Issue: 2 Pages: 363-372
  • DOI: 10.3201/eid2802.211532
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Host Immune Responses to Salivary Components - A Critical Facet of Tick-Host Interactions (2022)
  • Author(s): Ali, A., Zeb, I., Alouffi, AS., Zahid, H., Almutairi, M., Alshammari, FA., Termignoni, C., da Silva Vaz, I Jr. and Tanaka, T.
  • Journal Title: Frontiers in Cellular and Infection Microbiology Volume: 12 Pages: 809052-809052
  • DOI: 10.3389/fcimb.2022.809052
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular Detection and Characterization of Babesia and Theileria in Cattle and Water Buffaloes from Southern Luzon, Philippines (2022)
  • Author(s): Prado, I. C. B., Capuno Jr, L. X. B., Collera, P. D. L. P., Cabralda, A. P. D., De Ramos, K. A. S., Bernardo, J. M. G., Divina, B. P., Masatani, T., Tanaka, T. and Galay, R. L.
  • Journal Title: Microorganisms Volume: 10 Issue: 4 Pages: 678-678
  • DOI: 10.3390/microorganisms10040678
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Morphological identification and molecular characterization of economically important ticks (Acari: Ixodidae) from North and North-Western Egypt (2022)
  • Author(s): Senbill, H., Tanaka, T., Karawia, D., Rahman, S., Zeb, J., Sparagano, O., Baruah, A.
  • Journal Title: Acta Trop. Volume: 231 Pages: 106438-106438
  • DOI: 10.1016/j.actatropica.2022.106438
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Chromosome-Level Genome Sequence of Aspergillus puulaauensis MK2, a Fungus Isolated from a Dead Hard Tick (2021)
  • Author(s): Futagami, T., Mori, K., Kadooka, C., Niihara, H., Tashiro, K., Tamaki, H. and Tanaka, T.
  • Journal Title: Microbiology Resource Announcements Volume: 10 Issue: 36
  • DOI: 10.1128/mra.00372-21
  • Peer Reviewed
• [Journal Article] Development of a loop-mediated isothermal amplification (LAMP) assay targeting the mitochondrial cytochrome b gene for the rapid detection of alveolar echinococcosis in hepatic nodules of horses (2021)
  • Author(s): Hifumi, T., Akioka, K., Tanaka, T. and Miyoshi, N.
  • Journal Title: Veterinary Parasitology Volume: 299 Pages: 109573-109573
  • DOI: 10.1016/j.vetpar.2021.109573
  • Peer Reviewed
• [Journal Article] Molecular prevalence of Anaplasma marginale and Ehrlichia in domestic large ruminants and Rhipicephalus (Boophilus) microplus ticks from southern Luzon, Philippines. (2021)
  • Author(s): Remil Galay, Carina R Llaneta, Maria Karla Faye Monreal, Antero Armero, Arinanne Bel Baluyut, Czarina Marie Regino, Kristina Andrea Sandalo, Billy Vidina, Melbourne Talactac, Lennox Tapawan, Maarten Czar Mojares, Cherry Alvarez, Emmanuel Mago, Noemi Encarnacion, Masako Andoh, Tetsuya Tanaka
  • Journal Title: Frontiers in Veterinary Science Volume: 8 Pages: 746705-746705
  • DOI: 10.3389/fvets.2021.746705
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Life cycle of the tick, Haemaphysalis (Kaiseriana) bispinosa (Acari: Ixodidae) under laboratory conditions (2021)
  • Author(s): Senbill, H., Borah, D. K., Baruah, A., Tanaka,T. and Rahman, S.
  • Journal Title: International Journal of Acarology Volume: 48 Issue: 1 Pages: 7-14
  • DOI: 10.1080/01647954.2021.2009566
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] An Ixodes scapularis glutathione S-transferase plays a role in cell survival and viability during Langat virus infection of a tick cell line (2021)
  • Author(s): Hernandez Emmanuel Pacia、Talactac Melbourne Rio、Vitor Rodel Jonathan Santos、Yoshii Kentaro、Tanaka Tetsuya
  • Journal Title: Acta Tropica Volume: 214 Pages: 105763-105763
  • DOI: 10.1016/j.actatropica.2020.105763
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Determination of resistance status to amitraz in the cattle tick Rhipicephalus (Boophilus) microplus from Luzon, Philippines through bioassay and molecular analysis (2021)
  • Author(s): Alota S. L., Edquiban, T., Galay, R. L., Bernardo, J. M., Sandalo, K. A. C., Divina, B. P. and Tanaka, T.
  • Journal Title: Exp. Appl. Acarol. Volume: 83 Issue: 3 Pages: 399-409
  • DOI: 10.1007/s10493-021-00593-8
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The antiviral immunity of ticks against transmitted viral pathogens (2021)
  • Author(s): Talactac Melbourne Rio、Hernandez Emmanuel Pacia、Hatta Takeshi、Yoshii Kentaro、Kusakisako Kodai、Tsuji Naotoshi、Tanaka Tetsuya
  • Journal Title: Developmental & Comparative Immunology Volume: 119 Pages: 104012-104012
  • DOI: 10.1016/j.dci.2021.104012
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Molecular detection of Rickettsia spp. and Coxiella burnetii in cattle, water buffaloes, and Rhipicephalus (Boophilus) microplus ticks in Luzon Island of the Philippines (2020)
  • Author(s): Remil Galay, Melbourne Talactac, Bea Ambita, Dawn Chu, Lali dela Costa, Cinnamon Salangsang, Darwin Caracas, Florante Generoso, Jonathan Babelonia, Joeneil Vergano, Lena Berana, Kristina Sandalo, Billy Divina, Cherry Alvarez, Emman Mago, Masako Andoh, Tetsuya Tanaka
  • Journal Title: Trop. Med. Infect. Dis. Volume: 5(2) Issue: 2 Pages: 54-54
  • DOI: 10.3390/tropicalmed5020054
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Development of a Loop-mediated isothermal amplification (LAMP) assay targeting the citrate synthase gene for detection of Ehrlichia canis in dogs (2020)
  • Author(s): Chua, A. P. B., Galay, R. L., Tanaka, T. and Yamazaki, W.
  • Journal Title: Vet. Sci. Volume: 7 Issue: 4 Pages: 156-156
  • DOI: 10.3390/vetsci7040156
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Relationship between hepatic grayish-white solid nodules found in horses imported from Canada and larval Echinococcus multilocularis infection (2020)
  • Author(s): Hifumi, T., Tanaka, T., Hernandez, EP., Akioka, K., Yamada, K., Imamura, Y., Hatai, H. and Miyoshi, N.
  • Journal Title: Can. Vet. J. Volume: 62 Pages: 285-288
  • Peer Reviewed
• [Presentation] マダニ媒介性動物感染症の簡易迅速的な診断法の構築 (2024)
  • Author(s): 田仲哲也
  • Organizer: 令和5年度東南アジア地域研究研究所共同利用・共同研究拠点「グローバル共生に向けた東南アジア地域研究の国際共同研究拠点」年次研究成果発表会
  • Invited
• [Presentation] 鹿児島県大隅地域から検出されたRickettsia spp.におけるgltA, ompB, htrA遺伝子の分子疫学的特徴 (2023)
  • Author(s): 鈴 一之将, 木村仁美, 芦原誠一, 榮村奈緒子, 一二三達郎, 田仲哲也
  • Organizer: 令和5年度日本獣医公衆衛生学会(九州地区)
• [Presentation] 日本の食用馬の多包虫症における多包条虫のミトコンドリア・シトクロームb(cob)遺伝子を標的とした分子系統解析 (2023)
  • Author(s): 佐藤美穂, 一二三達郎, 田仲哲也, 鈴 一之将, 秋岡幸兵, 藤又千晶, 新飼竜平, 前田 勉, 草木迫浩大, 筏井宏実, 三好宣彰
  • Organizer: 令和5年度日本獣医公衆衛生学会(九州地区)
• [Presentation] 日本の食用馬における多包虫症のミトコンドリア・シトクロームb(cob)遺伝子に基づく分子疫学的特徴 (2023)
  • Author(s): 榮村奈緒子, 鈴 一之将, 横山雅大, 木村仁美, 芦原誠一, 畑 邦彦, 一二三達郎, 田仲哲也
  • Organizer: 第75回日本寄生虫学会南日本支部大会・第72回日本衛生動物学会南日本支部大会合同大会
• [Presentation] マダニから検出されたRickettsia spp.におけるgltA, ompB, htrA遺伝子の分子疫学的特徴 (2023)
  • Author(s): 鈴 一之将, 木村仁美, 芦原誠一, 榮村奈緒子, 一二三達郎, 田仲哲也
  • Organizer: 第75回日本寄生虫学会南日本支部大会・第72回日本衛生動物学会南日本支部大会合同大会
• [Presentation] リコンビナーゼポリメラーゼ増幅法を用いた馬の多包虫症における迅速診断法の確立 (2023)
  • Author(s): 佐藤美穂, 一二三達郎, 田仲哲也, 秋岡幸兵, 藤又千晶, 三好宣彰
  • Organizer: 鹿大細菌カンファレンス(第7回研究会)
• [Presentation] ナノポアシークエンサーを用いたマダニ種の同定とリケッチア種の迅速な塩基配列解読法の構築 (2023)
  • Author(s): 鈴 一之将, 木村仁美, 芦原誠一, 榮村奈緒子, 一二三達郎, 田仲哲也
  • Organizer: 鹿大細菌カンファレンス(第7回研究会)
• [Presentation] マダニ媒介性動物感染症の簡易迅速的な診断法の構築 (2023)
  • Author(s): 田仲哲也
  • Organizer: 令和4年度東南アジア地域研究研究所共同利用・共同研究拠点「グローバル共生に向けた東南アジア地域研究の国際共同研究拠点」年次研究成果発表会
  • Invited
• [Presentation] 鹿児島県大隅地域の異なる環境におけるマダニ類の出現状況とリケッチア保有率 (2023)
  • Author(s): 榮村奈緒子, 横山雅大, 畑邦彦, 芦原誠一, 鈴一之将, 田仲哲也
  • Organizer: 第70回日本生態学会大会
• [Presentation] リコンビナーゼポリメラーゼ増幅を用いた牛の小型ピロプラズマ病の迅速的な遺伝子検出法の開発 (2023)
  • Author(s): 樋ノ口峰大, 叶 有斗, 志賀 慧, 堀江真行, 一二三達郎, 田仲哲也
  • Organizer: 第92回日本寄生虫学会大会
• [Presentation] 馬の多包虫症におけるリコンビナーゼポリメラーゼ増幅法を用いた迅速な診断方法の開発 (2022)
  • Author(s): 佐藤美穂, 一二三達郎, 田仲哲也, 秋岡幸兵, 藤又千晶, 三好宣彰
  • Organizer: 令和4年度日本獣医公衆衛生学会(九州地区)
• [Presentation] リコンビナーゼポリメラーゼ増幅法を用いた牛のTheileria orientalis遺伝子型の同定 (2022)
  • Author(s): 樋ノ口 峰大, 叶 有斗, 志賀 慧, 堀江真行, 一二三 達郎, 田仲哲也
  • Organizer: 令和4年度日本産業動物獣医学会(九州地区)
• [Presentation] 鹿児島県大隅地域におけるRickettsia spp.の疫学調査ならびにgltA遺伝子を標的とした分子系統解析 (2022)
  • Author(s): 鈴 一之将, 横山雅大, 芦原誠一, 榮村奈緒子, 田仲哲也
  • Organizer: 74回日本寄生虫学会南日本支部大会・第71回日本衛生動物学会南日本支部大会合同大会
• [Presentation] 馬の多包虫症の迅速な検出を目的としたリコンビナーゼポリメラーゼ増幅法による新しい診断法の開発 (2022)
  • Author(s): 一二三達郎, 田仲哲也, 佐藤美穂, 秋岡幸兵, 藤又千晶, 三好宣彰
  • Organizer: 第74回日本寄生虫学会南日本支部大会・第71回日本衛生動物学会南日本支部大会合同大会
• [Presentation] 牛のTheileria orientalisにおけるリコンビナーゼポリメラーゼ増幅法を用いた迅速的な診断法の開発 (2022)
  • Author(s): 樋ノ口 峰大, 叶 有斗, 志賀 慧, 堀江真行, 一二三 達郎, 田仲哲也
  • Organizer: 鹿大細菌カンファレンス(第6回研究会)
• [Presentation] 大隅地域におけるRickettsia spp.の疫学調査ならびにgltA遺伝子を標的とした分子系統解析 (2022)
  • Author(s): 鈴 一之将, 横山雅大, 芦原誠一, 榮村奈緒子, 一二三達郎, 田仲哲也
  • Organizer: 鹿大細菌カンファレンス(第6回研究会)
• [Presentation] Development of a real-time recombinase polymerase amplification (RPA) for the rapid detection of alveolar echinococcosis in hepatic nodules of horses (2022)
  • Author(s): Tatsuro Hifumi, Tetsuya Tanaka, Miho Sato, Kohei Akioka, Chiaki Fujimata, Noriaki Miyoshi
  • Organizer: The 21st Federation of Asian Veterinary Associations(FAVA) Congress
  • Int'l Joint Research
• [Presentation] 馬の多包虫症におけるLAMP法を用いた迅速的な診断方法の開発 (2022)
  • Author(s): 一二三達郎, 秋岡幸兵, 田仲哲也, 三好宣彰
  • Organizer: 令和3年度日本獣医師会獣医学術学会年次大会 日本獣医師会
  • Invited
• [Presentation] 高濃度のウイルス血症を伴った猫の致死的SFTSにおける臨床および病理学的解析 (2021)
  • Author(s): 松鵜 彩, 畑井 仁, 一二三達郎, 田仲哲也, 濱久保咲夢, 嶽 真帆, 桃井康之, 遠藤泰之, 川畑貴裕
  • Organizer: 第3回SFTS研究会学術集会
• [Presentation] 鹿児島県におけるTheileria orientalisの分子系統解析 (2021)
  • Author(s): 樋ノ口 峰大, 叶 有斗, 志賀 慧, 一二三 達郎, 田仲哲也
  • Organizer: 第73回日本寄生虫学会南日本支部大会・第70回日本衛生動物学会南日本支部大会合同大会
• [Presentation] 馬の多包虫症を迅速に検出するための多包条虫のミトコンドリア・シトクロムb遺伝子を標的とするLAMP法の開発 (2021)
  • Author(s): 一二三達郎, 秋岡幸兵, 田仲哲也, 三好宣彰
  • Organizer: 第73回日本寄生虫学会南日本支部大会・第70回日本衛生動物学会南日本支部大会合同大会
• [Presentation] 馬の多包虫症におけるLAMP法を用いた迅速的な診断方法の開発 (2021)
  • Author(s): 一二三達郎, 秋岡幸兵, 田仲哲也, 三好宣彰
  • Organizer: 令和3年度日本獣医公衆衛生学会(九州地区) 一般社団法人宮崎県獣医師会
• [Presentation] Promoter analysis of elongation factor 1-alpha (EF1α) derived from Haemaphysalis longicornis (2021)
  • Author(s): Hernandez Emmanuel Pacia, 川田逸人, 山本大介, 加藤大智, 松林 誠, 辻 尚利, 田仲哲也, 八田岳士 .
  • Organizer: 第90回日本寄生虫学会・第32回日本臨床寄生虫学会合同大会
• [Presentation] マダニフェリチンの機能解明 (2021)
  • Author(s): 田仲哲也
  • Organizer: 病害動物の生理分子生物談話会(第29回)
  • Invited
• [Presentation] マダニ胚由来細胞におけるフタトゲチマダニプロモータ活性の評価 (2020)
  • Author(s): Hernandez Emmanuel Pacia, 草木迫浩大, 八田岳士, 田仲哲也
  • Organizer: 第43回日本分子生物学会年会
• [Remarks] 鹿児島大学共同獣医学部獣医学科病態予防獣医学講座感染症学分野
  • URL: http://www.vet.kagoshima-u.ac.jp/kadai/V-Infection/index.html
• [Remarks] 研究者総覧
  • URL: https://ris.kuas.kagoshima-u.ac.jp/html/100004666_ja.html
• [Remarks] ResearchGate
  • URL: https://www.researchgate.net/profile/Tetsuya-Tanaka-4
• [Remarks] Researchmap
  • URL: https://researchmap.jp/read0065424?lang=ja
• [Remarks] 研究者総覧
  • URL: https://ris.kuas.kagoshima-u.ac.jp/search?m=home&l=ja
• [Remarks] 鹿児島大学 共同獣医学部獣医学科 病態予防獣医学講座 感染症学分野
  • URL: http://www.vet.kagoshima-u.ac.jp/kadai/V-Infection/index.html