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Marine environmental DNA metabarcoding: understanding oceanic islands and mainland sites across anthropogenic gradients in two hemispheres

Research Project

Project/Area Number 20KK0164
Research Category

Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 45:Biology at organismal to population levels and anthropology, and related fields
Research InstitutionUniversity of the Ryukyus

Principal Investigator

REIMER JD  琉球大学, 理学部, 教授 (20452956)

Co-Investigator(Kenkyū-buntansha) 成瀬 貫  琉球大学, 熱帯生物圏研究センター, 准教授 (30398309)
櫛田 優花  立正大学, 地球環境科学部, 助教 (90914341)
MASUCCI GIOVANNI・DIEGO  琉球大学, 理工学研究科, 博士研究員 (80890053)
Project Period (FY) 2020-10-27 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2023: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2022: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2021: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2020: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
KeywordseDNA / degradation / anthropogenic impacts / coral reefs / marine biodiversity / coral / fish / oceanic islands / Ogasawara / marine ecosystem / anthropogenic impact / oceanic island / biodiversity / anthropogenic pressure / marine ecosystems
Outline of Research at the Start

We will utilize environmental DNA data over four years from pristine and anthropogenically impacted sites in Australia and Japan to 1) examine total coral community biodiversity from microbes to megafauna, 2) examine how biodiversity in oceanic regions is linked with mainland sites, and 3) examine how human impacts affect biodiversity and linkages between these sites.

Outline of Final Research Achievements

To investigate whether oceanic islands experience the same patterns of degradation as mainland sites, we examined the environmental DNA of sites on the Japanese mainland and Ogasawara. We conducted field work in Ogasawara with Australian collaborators and collected eDNA samples around southern Japan. Our dataset shows that human-impacted sites are not necessarily less diverse, but rather may have different biodiversity. Initial analysis shows that the impacted sites have commonalities across different regions and sites, indicating that perhaps anthropogenic impacts are all altering marine ecosystems in specific ways, suggesting that a general theory of marine ecosystem degradation due to anthropogenic impacts may be within reach.

Academic Significance and Societal Importance of the Research Achievements

Our analyses indicate there are common denominators to impacted sites across different sites, showing that anthropogenic impacts all change marine ecosystems in particular ways, and there may be some general theory of marine ecosystem degradation under anthropogenic influences within reach.

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (18 results)

All 2024 2023 2022 2020 Other

All Int'l Joint Research (10 results) Journal Article (3 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (5 results) (of which Int'l Joint Research: 2 results,  Invited: 4 results)

  • [Int'l Joint Research] University of Newcastle/Griffith University(オーストラリア)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] Australian Museum/University of Newcastle/Curtin University(オーストラリア)

    • Related Report
      2022 Research-status Report
  • [Int'l Joint Research] Victoria University of Wellington/Environmental Protection Authority/Cawthorn Institute(ニュージーランド)

    • Related Report
      2022 Research-status Report
  • [Int'l Joint Research] University of Leeds/University of Essex(英国)

    • Related Report
      2022 Research-status Report
  • [Int'l Joint Research] Australian Museum/University of Newcastle/Curtin University(オーストラリア)

    • Related Report
      2021 Research-status Report
  • [Int'l Joint Research] Victoria University of Wellington/Environmental Protection Authority(ニュージーランド)

    • Related Report
      2021 Research-status Report
  • [Int'l Joint Research] University of Leeds(英国)

    • Related Report
      2021 Research-status Report
  • [Int'l Joint Research] Australian Museum/University of Newcastle/Curtin University(オーストラリア)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] Victoria University of Wellington/Environmental Protection Authority(ニュージーランド)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] University of Leeds(英国)

    • Related Report
      2020 Research-status Report
  • [Journal Article] Fish and coral assemblages of a highly isolated oceanic island: The first e<scp>DNA</scp> survey of the Ogasawara Islands2024

    • Author(s)
      Acikbas Ayse Haruka Oshima、Narisoko Haruhi、Huerlimann Roger、Nishitsuji Koki、Satoh Noriyuki、Reimer James Davis、Ravasi Timothy
    • Journal Title

      Environmental DNA

      Volume: 6 Issue: 1

    • DOI

      10.1002/edn3.509

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Can environmental DNA unlock the mysteries of biodiversity on coral reefs?2023

    • Author(s)
      Reimer James Davis、Goesser Fabian
    • Journal Title

      Proceedings of the Royal Society B: Biological Sciences

      Volume: 290 Issue: 1998

    • DOI

      10.1098/rspb.2023.0605

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Environmental DNA can act as a biodiversity barometer of anthropogenic pressures in coastal ecosystems2020

    • Author(s)
      DiBattista Joseph D.、Reimer James D.、Stat Michael、Masucci Giovanni D.、Biondi Piera、De Brauwer Maarten、Wilkinson Shaun P.、Chariton Anthony A.、Bunce Michael
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 8365-8365

    • DOI

      10.1038/s41598-020-64858-9

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Using eDNA to understand the diversity of and anthropogenic impacts on coral reefs in southern Japan2023

    • Author(s)
      JD Reimer and colleagues
    • Organizer
      eDNA Society
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] Marine biodiversity patterns in an anthropogenically impacted oceanic archipelago: The first eDNA survey of the Ogasawara Islands2023

    • Author(s)
      AHO Acikbas et al. (incl. JD Reimer)
    • Organizer
      1st Australian & New Zealand eDNA conference
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] Okinawa, Japan: A Model Location for Examining Anthropogenic Impacts on Coral Reefs2022

    • Author(s)
      JD Reimer et al.
    • Organizer
      Nha Trang University public lecture
    • Related Report
      2022 Research-status Report
    • Invited
  • [Presentation] Okinawa, Japan: A Model Location for Examining Anthropogenic Impacts on Coral Reefs2022

    • Author(s)
      James Davis Reimer, Hirotaka Yamagiwa, Katie Midori Cook, Joseph D. DiBattista, Megan Huggett, Michael Stat, James Cant, Anthony A. Chariton, Maarten De Brauwer, Shaun P. Wilkinson, Giovanni Diego Masucci, Piera Biondi, Stuart Ross, Lee Hui Yian Theodora, Rick D. Stuart-Smith, Michael Bunce, Maria Beger
    • Organizer
      The 2nd International Conference on Biodiversity, Ecology and Conservation of Marine Ecosystems
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] サンゴ礁の礫の潜在的重要性と環境DNA技術2022

    • Author(s)
      James Davis Reimer, Piera Biondi, Giovanni Masucci
    • Organizer
      さんご i n サンゴ礁ウィーク 2 0 2 2 の海オンラインフエスタ
    • Related Report
      2021 Research-status Report
    • Invited

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Published: 2020-10-29   Modified: 2026-01-16  

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