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Revealing the ecophysiologies of nitrous oxide-reducing bacteria towards the mitigation of greenhouse gas emissions in advanced nitrogen removal processes for wastewater treatment

Research Project

Project/Area Number 20KK0243
Research Category

Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 64:Environmental conservation measure and related fields
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

Terada Akihiko  東京農工大学, 工学(系)研究科(研究院), 教授 (30434327)

Co-Investigator(Kenkyū-buntansha) 黒岩 恵  東京農工大学, 工学(系)研究科(研究院), 助教 (00761024)
徳山 英昭  東京農工大学, 工学(系)研究科(研究院), 准教授 (10363029)
末永 俊和  広島大学, 先進理工系科学研究科(工), 助教 (80828377)
安田 昌平  東京農工大学, 学内共同利用施設等, 産学官連携研究員 (10910672)
Project Period (FY) 2020-10-27 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2023: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2022: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2021: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2020: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords亜酸化窒素 (N2O) / N2O還元細菌 / Anammox細菌 / N2O消費速度 / メタゲノム解析 / 脱窒細菌 / 集積培養 / 15Nトレーサー / 亜酸化窒素 / 15Nトレーサー法 / 嫌気性アンモニア酸化 / 脱窒遺伝子 / メタトランスクリプトーム解析 / ゲル固定化 / 動力学的解析 / メタゲノム / 転写活性 / 電子伝達 / 脱窒 / 高度窒素除去システム / 電子競合 / 遺伝子発現 / 高度窒素除去
Outline of Research at the Start

本研究は、強い温室効果を示すガスである亜酸化窒素(N2O)の排出を削減するため、N2Oを消費して無害な窒素ガスに変換可能な細菌群の種類、機能、性能を明らかにすることを目的とする。特に、窒素を除去する高度排水処理施設に潜む高活性N2O還元細菌を独自培養装置で獲得し、最先端の解析により機能解明を行う。さらに、細菌を高密度に固定化する技術を開発することで、排水処理施設からのN2O排出の削減を目指す。

Outline of Final Research Achievements

Energy-saving nitrogen removal from wastewaters occasionally offsets the mitigation of greenhouse gas nitrous-oxide (N2O) emissions. To break down the trade-off, this study aimed at exploring uncultured N2O-reducing bacteria, understanding their ecophysiologies, culturing them, and harnessing them to mitigate N2O emissions from engineered systems. A fine-tuned 15N tracer method disentangled the potential of N2O-reducing bacteria and their limiting factors. Metagenomic analysis revealed that the majority of N2O-reducing bacteria in energy-saving nitrogen removal processes have a non-denitrifying nature, lacking some denitrifying genes, and a trait possessing diversified genes to synthesize vitamin B12, essential for methionine biosynthesis. Long-term enrichment, followed by isolation, attained non-denitrifying N2O-reducing bacteria. Furthermore, a design of a suitable gel-entrapment technology immobilizing N2O-reducing bacteria for N2O mitigation was established.

Academic Significance and Societal Importance of the Research Achievements

富栄養化の原因物質の窒素化合物を省エネで除去可能なAnammoxプロセスは、場合によって強力な温室効果ガスである亜酸化窒素(N2O)の排出を増長させるトレードオフを有している。N2Oは排水処理施設のCO2フットプリントの大部分を占めることが知られており、排出削減が必要不可欠である。本研究では、省エネ型窒素除去プロセスにおいて安定的にN2Oを除去可能なN2O還元細菌の深層理解と有用種の獲得、および削減技術開発の基盤構築を達成した。このような成果により、従来の課題であった排水処理の省エネ化と温室効果ガスの排出削減を両立し、温室効果ガスの削減を達成する技術確立に寄与できることが期待される。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (17 results)

All 2024 2023 2022 2021 Other

All Int'l Joint Research (6 results) Journal Article (3 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (8 results) (of which Int'l Joint Research: 7 results,  Invited: 3 results)

  • [Int'l Joint Research] ダルムシュタット工科大学(ドイツ)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] ゴールウェイ大学(アイルランド)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] ダルムシュタット工科大学(ドイツ)

    • Related Report
      2022 Research-status Report
  • [Int'l Joint Research] ダルムシュタット工科大学(ドイツ)

    • Related Report
      2021 Research-status Report
  • [Int'l Joint Research] デンマーク工科大学(デンマーク)

    • Related Report
      2021 Research-status Report
  • [Int'l Joint Research] コロンビア大学(米国)

    • Related Report
      2021 Research-status Report
  • [Journal Article] Complete genome sequence of Afipia carboxidovorans strain SH125, a non-denitrifying nitrous oxide-reducing bacterium isolated from anammox biomass2024

    • Author(s)
      Oba Kohei、Yasuda Shohei、Terada Akihiko
    • Journal Title

      Microbiology Resource Announcements

      Volume: 13 Issue: 3

    • DOI

      10.1128/mra.01279-23

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Quest for Nitrous Oxide-reducing Bacteria Present in an Anammox Biofilm Fed with Nitrous Oxide2024

    • Author(s)
      Oba Kohei、Suenaga Toshikazu、Yasuda Shohei、Kuroiwa Megumi、Hori Tomoyuki、Lackner Susanne、Terada Akihiko
    • Journal Title

      Microbes and Environments

      Volume: 39 Issue: 1 Pages: n/a

    • DOI

      10.1264/jsme2.ME23106

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Combination of 15N Tracer and Microbial Analyses Discloses N2O Sink Potential of the Anammox Community2021

    • Author(s)
      Suenaga Toshikazu、Ota Takumi、Oba Kohei、Usui Kentaro、Sako Toshiki、Hori Tomoyuki、Riya Shohei、Hosomi Masaaki、Chandran Kartik、Lackner Susanne、Smets Barth F.、Terada Akihiko
    • Journal Title

      Environmental Science & Technology

      Volume: 55 Issue: 13 Pages: 9231-9242

    • DOI

      10.1021/acs.est.1c00674

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] ガス透過膜を用いた集積培養装置の亜酸化窒素還元細菌群の集積化培養への利用2024

    • Author(s)
      大場 康平、寺田 昭彦
    • Organizer
      化学工学会第89年会
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Effect of Calcium Alginate Gel Concentration on Mitigation of N2O Emission by Gel Entrapped Azospira sp. strain I13: A Biokinetic Study2023

    • Author(s)
      Jannah Salsabil, Tokuyama Hideaki, Terada Akihiko
    • Organizer
      Water Technology Conference 2023 (online)
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Organic carbon species selects predominant N2O-reducing bacteria: The insights from biokinetics and genomics of canonical denitrifying bacteria2023

    • Author(s)
      Terada Akihiko
    • Organizer
      18th IWA Leading Edge Conference on Water and Wastewater Technologies
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Phylogeny, abundance, and function of N2O-reducing bacteria in an anammox biofilm2023

    • Author(s)
      Oba Kohei, Kuroiwa Megumi, Terada Akihiko
    • Organizer
      The Eighth International Conference on Nitrification and Related Processes
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Anammoxプロセスの温室効果ガス削減に貢献するN2O還元細菌の探索と機能解明2023

    • Author(s)
      大場康平, 黒岩恵, 堀知行, 寺田昭彦
    • Organizer
      第57回日本水環境学会年会
    • Related Report
      2022 Research-status Report
  • [Presentation] A15N tracer method reveals canonical denitrifiers, Azospira spp., as a nitrous oxide consumer in the presence of nitrate2022

    • Author(s)
      Kohei Oba, Toshikazu Suenaga, Megumi Kuroiwa, Shohei Riya, Akihiko Terada
    • Organizer
      18th International Symposium on Microbial Ecology
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] Harnessing microbial communities for bioenergy recovery;greenhouse gas mitigations2022

    • Author(s)
      Akihiko Terada
    • Organizer
      The 6th International Conference on Integrated and Innovative Solutions for Circular Ecolony
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] A novel biofilm reactor using a gas-permeable membrane for cost-effective nitrogen removal: Challenges and opportunities2021

    • Author(s)
      Akihiko Terada
    • Organizer
      Sustainable Development on Water Reclamation and Reuse
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited

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Published: 2020-10-29   Modified: 2025-01-30  

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