|Budget Amount *help
¥510,900,000 (Direct Cost: ¥393,000,000、Indirect Cost: ¥117,900,000)
Fiscal Year 2013: ¥122,720,000 (Direct Cost: ¥94,400,000、Indirect Cost: ¥28,320,000)
Fiscal Year 2012: ¥123,500,000 (Direct Cost: ¥95,000,000、Indirect Cost: ¥28,500,000)
Fiscal Year 2011: ¥128,050,000 (Direct Cost: ¥98,500,000、Indirect Cost: ¥29,550,000)
Fiscal Year 2010: ¥136,630,000 (Direct Cost: ¥105,100,000、Indirect Cost: ¥31,530,000)
|Outline of Final Research Achievements
A powerful means for understanding the mechanism of a protein molecular machine is single-molecule physiology where one directly watches the behaviors of individual molecules under a microscope. One may apply a force to learn from the response of the machine, but, in most cases so far, the force was in an impeding direction. Here, we propose to reach a better understanding by applying an external force to a machine “to let it work” properly. With this and other approaches we have been able to elucidate the mechanism of ATP synthesis effected by reverse rotation of a rotary molecular motor, and various mechanistic aspects of rotary and linear molecular motors, protein machines that work on DNA, and the supramolecular machinery for cell division.