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Analysis of the mechanisms of the synapse formation of newborn neurons by developing mice with channelrhodopsin-2 only in newborn neuron in the dentate gyrus.

Research Project

Project/Area Number 21200016
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)

Allocation TypeSingle-year Grants
Research Field Neurophysiology and muscle physiology
Psychiatric science
Research InstitutionGunma University

Principal Investigator

YASUDA Hiroki  群馬大学, 大学院・医学系研究科, 准教授 (60294071)

Co-Investigator(Renkei-kenkyūsha) KANEKO Ryosuke  群馬大学, 大学院・医学系研究科, 助教 (40390695)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥30,940,000 (Direct Cost: ¥23,800,000、Indirect Cost: ¥7,140,000)
Fiscal Year 2011: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2010: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2009: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
Keywords神経新生 / 海馬歯状回 / チャネルロドプシン-2 / トランスジェニックマウス / 新生神経細胞 / チャネルロドプシン2 / 抗うつ剤 / ストレス / PKN / カンナビノイド / シナプス可塑性
Research Abstract

We planned to develop a new genetically modified mice whose newborn neurons in the dentate gyrus in the hippocampus posess channelrhodopsin-2 (chr2) to analyze the mechanisms of synapse formation of newborn neurons. We are still in the middle of generating the chr2 mice we originally planned, instead we used another mice in which neurons with markers of inhibitory cells express channelrhodopsin-2, because newborn excitatory neurons in the dentate gyrus also have these markers until about three weeks after birth. We now check whether light stimulation on the dentate gyrus evoked EPSCs in pyramidal neurons in the CA3 region and inhibitory neurons in the hilus.
Recent literature suggests that antidepressants improve depressed mood through increasing generation of newborn neurons in the dentate gyrus in the hippocampus, therefore we examined the effects of stress, which can cause depression, on the excitability of granule cells in the dentate gyrus. We found that forced swimming made granule neurons more excitable and now investigating the mechanisms that underlie the elevated excitability.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (27 results)

All 2012 2011 2010 2009 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (18 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Selective control of inhibitory synapse development by Slitrk3-PTPδ trans-synaptic interaction2012

    • Author(s)
      Hideto Takahashi, Kei-ichi Katayama, Kazuhiro Sohya, Hiroyuki Miyamoto, Tuhina Prasad, Yoshifumi Matsumoto, Maya Ota, Hiroki Yasuda, Tadaharu Tsumoto, Jun Aruga & Ann Marie Craig
    • Journal Title

      Nature Neuroscience

      Volume: 15(3) Pages: 389-398

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Selective control of inhibitory synapse development by Slitrk3-PTPδ trans-synaptic interaction2012

    • Author(s)
      Hideto Takahashi, Kei-ichi Katayama, Kazuhiro Sohya, Hiroyuki Miyamoto, Tuhina Prasad, Yoshifumi Matsumoto, Maya Ota, Hiroki Yasuda, Tadaharu Tsumoto, Jun Aruga, Ann Marie Craig
    • Journal Title

      Nature Neuroscience

      Volume: 15 Issue: 3 Pages: 389-398

    • DOI

      10.1038/nn.3040

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of the Cluster Control Region for the Protocadherin-β Genes Located beyond the Protocadherin-γ Cluster.2011

    • Author(s)
      Shinnichi Yokota, Teruyoshi Hirayama, Keizo Hirano, Ryosuke Kaneko, Shunsuke Toyoda, Yoshimi Kawamura, Masumi Hirabayashi, Takahiro Hirabayashi and Takeshi Yagi
    • Journal Title

      J. Biol. Chem.

      Volume: 286 Pages: 31885-31895

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Frequent spontaneous seizures followed by spatial working memory/anxiety deficits in mice lacking sphingosine 1-phosphate receptor 2.2011

    • Author(s)
      Noriyuki Akahoshi, Yasuki Ishizaki, Hiroki Yasuda, Yoshiya L. Murashima, Toshikazu Shinba, Kaoru Goto, Toshiyuki Himi, Jerold Chun, Isao Ishii
    • Journal Title

      Epilepsy & Behavior

      Volume: 22(4) Pages: 659-665

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Frequent spontaneous seizures followed by spatial working memory/anxiety deficits in mice lacking sphingosine 1-phosphate receptor 22011

    • Author(s)
      Noriyuki Akahoshi, Yasuki Ishizaki, Hiroki Yasuda, Yoshiya L.Murashima, Toshikazu Shinba, Kaoru Goto, Toshiyuki Himi, Jerold Chun, Isao Ishii
    • Journal Title

      Epilepsy & Behavior

      Volume: 22 Issue: 4 Pages: 659-665

    • DOI

      10.1016/j.yebeh.2011.09.002

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression levels of Protocadherin-α transcripts are decreased by nonsense-mediated mRNA decay with frameshift mutations and by high DNA methylation in their promoter regions.2009

    • Author(s)
      Ryosuke Kaneko, Masahumi Kawaguchi, Tomoko Toyama, Yusuke Taguchi, Takeshi Yagi
    • Journal Title

      Gene

      Volume: 430 Pages: 86-94

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Presentation] 発達期海馬長期抑圧誘発におけるカルシウムソースのスイッチ Turning on of CICR in LTD induction after critical period of NR2 subunits switch2011

    • Author(s)
      安田 浩樹
    • Organizer
      第34回日本神経科学学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2011-09-16
    • Related Report
      2011 Final Research Report
  • [Presentation] 発達期海馬長期抑圧誘発におけるカルシウムソースのスイッチ2011

    • Author(s)
      安田浩樹
    • Organizer
      第34回日本神経科学学会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2011-09-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Lrfn2/SALM1 による海馬興奮性シナプスの機能制御とその遺伝子欠損マウスにみられた行動異常2011

    • Author(s)
      守村 直子, 安田 浩樹, 山田 一之, 富岡 直子, 片山 圭一, 山口 和彦, 太田 摩耶, 神谷 明子, 有賀 純
    • Organizer
      第34回日本神経科学学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2011-09-15
    • Related Report
      2011 Final Research Report
  • [Presentation] Lrfn2/SALM1による海馬興奮性シナプスの機能制御とその遺伝子欠損マウスにみられた行動異常2011

    • Author(s)
      守村直子, 安田浩樹, 山田一之, 富岡直子, 片山圭一, 山口和彦, 太田摩耶, 神谷明子, 有賀純
    • Organizer
      第34回日本神経科学学会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2011-09-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] REGULATORY ROLE OF DREBRIN IN HIPPOCAMPUSDEPENDENT LEARNING ISN-ESN-20112011

    • Author(s)
      Shirao, T., Hanamura, K., Yasuda, H., Kajita, Y., Kamata, Y., Sekino, Y. and Kojima, N.
    • Organizer
      (International Society for Neurochemistry and European Society for Neurochemistry) 23^<rd> Biennial Meeting
    • Place of Presentation
      Athens, Greece
    • Year and Date
      2011-08-30
    • Related Report
      2011 Final Research Report
  • [Presentation] REGULATORY ROLE OF DREBRIN IN HIPPOCAMPUSDEPENDENT LEARNING2011

    • Author(s)
      Shirao, T., Hanamura, K., Yasuda, H., Kajita, Y., Kamata, Y., Sekino, Y., Kojima, N.
    • Organizer
      ISN-ESN-2011 (International Society for Neurochemistry and European Society for Neurochemistry) 23^<rd> Biennial Meeting
    • Place of Presentation
      Athens, Greece
    • Year and Date
      2011-08-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] Isoform conversion of drebrin is required for hippocampus-dependent learning and synaptic plasticity in the elderly ドレブリンのアイソフォーム変換は海馬依存的学習とシナプス可塑性に必要である2011

    • Author(s)
      児島 伸彦、安田 浩樹、花村 健次、白尾 智明
    • Organizer
      第84回日本薬理学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2011-03-23
    • Related Report
      2011 Final Research Report
  • [Presentation] ドレブリンのアイソフォーム変換は海馬依存的学習とシナプス可塑性に必要である2011

    • Author(s)
      児島伸彦、安田浩樹, 等
    • Organizer
      第84回日本薬理学会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2011-03-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] Neuron-specific drebrin isoform is necessary for hippocampus-dependent learning in adulthood but not in young age of mice2010

    • Author(s)
      N. KOJIMA, H. YASUDA, K. HANAMURA, T. SHIRAO
    • Organizer
      Neuroscience 2010 Society for Neuroscience 40 th Annual Meeting
    • Place of Presentation
      San Diego Convention Center, San Diego, USA
    • Year and Date
      2010-11-16
    • Related Report
      2011 Final Research Report
  • [Presentation] Neuron-specific drebrin isoform is necessary for hippocampus-dependent learning in adulthood but not in young age of mice2010

    • Author(s)
      N.KOJIMA, H.YASUDA, et al.
    • Organizer
      Neuroscience 2010 Society for Neuroscience 40^<th> Annual Meeting
    • Place of Presentation
      San Diego Convention Center, San Diego, USA
    • Year and Date
      2010-11-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] ドレブリンアイソフォーム変換不全マウスにおける海馬シナプス可塑性と海馬依存的学習の障害2010

    • Author(s)
      児島 伸彦, 安田 浩樹, 花村 健次, 白尾 智明
    • Organizer
      Neuro2010 第 33 回日本神経科学学会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2010-09-04
    • Related Report
      2011 Final Research Report
  • [Presentation] ドレブリンアイソフォーム変換不全マウスにおける海馬シナプス可塑性と海馬依存的学習の障害2010

    • Author(s)
      児島伸彦、安田浩樹, 等
    • Organizer
      Neuro2010第33回日本神経科学学会
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Year and Date
      2010-09-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] Lrfn2/SALM1, a synaptic leucine-rich repeat transmembrane molecule is required for proper synapse maturation and brain function2009

    • Author(s)
      Naoko Morimura, Maya Ota, Hiroki Yasuda, Kei-ichi Katayama, Naoko Hara, Kazuyuki Yamada, Kazuhiko Yamaguchi, Jun Aruga
    • Organizer
      The 32 nd Annual Meetings of the Japan Neuroscience Society P1-b23
    • Place of Presentation
      名古屋国際会議場、Nagoya
    • Year and Date
      2009-09-17
    • Related Report
      2011 Final Research Report
  • [Presentation] Lrfn2/SALM1, a synaptic leucine-rich repeat transmembrane molecule is required for proper synapse maturation and brain function2009

    • Author(s)
      守村直子、太田摩耶、安田浩樹, 他
    • Organizer
      第32回日本神経科学大会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-09-17
    • Related Report
      2009 Annual Research Report
  • [Presentation] Synaptic maturation by PKN during development2009

    • Author(s)
      Hiroki Yasuda, Yishitaka Ono, Hideyuki Mukai
    • Organizer
      The 32 nd Annual Meetings of the Japan Neuroscience Society P1-b23
    • Place of Presentation
      名古屋国際会議場、Nagoya
    • Year and Date
      2009-09-16
    • Related Report
      2011 Final Research Report
  • [Presentation] Synaptic maturation by PKN during development2009

    • Author(s)
      安田浩樹
    • Organizer
      第32回日本神経科学大会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-09-16
    • Related Report
      2009 Annual Research Report
  • [Presentation] SPECIFIC ROLE OF NEURONAL ISOFORM OF DREBRIN IN HIPPOCAMPAL SYNAPTIC PLASTICITY AND FEAR MEMORY2009

    • Author(s)
      Nobuhiko Kojima, Hiroki Yasuda, Kenji Hanamura, Hiroyuki Yamazaki, Tomoaki Shirao
    • Organizer
      The XXXVI International Congress of Physiological Sciences (IUPS2009) P1PM-7-6 (Kyoto)
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2009-07-28
    • Related Report
      2011 Final Research Report
  • [Presentation] SPECIFIC ROLE OF NEURONAL ISOFORM OF DREBRIN IN HIPPOCAMPAL SYNAPTIC PLASTICITY AND FEAR MEMORY2009

    • Author(s)
      Kojima, Nobuhiko, Hiroki Yasuda, et al.
    • Organizer
      The XXXVI International Congress of Physiological Sciences(IUPS2009)
    • Place of Presentation
      京都
    • Year and Date
      2009-07-28
    • Related Report
      2009 Annual Research Report
  • [Book] Electrophysiological technique for analysis of synaptic function of PKN1 in hippocampus. Protein Kinase Technologies, Neuromethods vol.68, (Mukai H, eds.)2012

    • Author(s)
      Hiroki Yasuda and Hideyuki Mukai
    • Publisher
      Springer, 2012
    • Related Report
      2011 Final Research Report
  • [Book] Neuromethods : Protein Kinase Technologies2011

    • Author(s)
      H.Yasuda, H.Mukai
    • Publisher
      Springer(in press)
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://ercgsm.dept.med.gunma-u.ac.jp/?page_id=541

    • Related Report
      2011 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2018-03-28  

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