Analysis of the mechanisms of the synapse formation of newborn neurons by developing mice with channelrhodopsin-2 only in newborn neuron in the dentate gyrus.
Project/Area Number |
21200016
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Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
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Allocation Type | Single-year Grants |
Research Field |
Neurophysiology and muscle physiology
Psychiatric science
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Research Institution | Gunma University |
Principal Investigator |
YASUDA Hiroki 群馬大学, 大学院・医学系研究科, 准教授 (60294071)
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Co-Investigator(Renkei-kenkyūsha) |
KANEKO Ryosuke 群馬大学, 大学院・医学系研究科, 助教 (40390695)
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Project Period (FY) |
2009 – 2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥30,940,000 (Direct Cost: ¥23,800,000、Indirect Cost: ¥7,140,000)
Fiscal Year 2011: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2010: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2009: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
|
Keywords | 神経新生 / 海馬歯状回 / チャネルロドプシン-2 / トランスジェニックマウス / 新生神経細胞 / チャネルロドプシン2 / 抗うつ剤 / ストレス / PKN / カンナビノイド / シナプス可塑性 |
Research Abstract |
We planned to develop a new genetically modified mice whose newborn neurons in the dentate gyrus in the hippocampus posess channelrhodopsin-2 (chr2) to analyze the mechanisms of synapse formation of newborn neurons. We are still in the middle of generating the chr2 mice we originally planned, instead we used another mice in which neurons with markers of inhibitory cells express channelrhodopsin-2, because newborn excitatory neurons in the dentate gyrus also have these markers until about three weeks after birth. We now check whether light stimulation on the dentate gyrus evoked EPSCs in pyramidal neurons in the CA3 region and inhibitory neurons in the hilus. Recent literature suggests that antidepressants improve depressed mood through increasing generation of newborn neurons in the dentate gyrus in the hippocampus, therefore we examined the effects of stress, which can cause depression, on the excitability of granule cells in the dentate gyrus. We found that forced swimming made granule neurons more excitable and now investigating the mechanisms that underlie the elevated excitability.
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Report
(4 results)
Research Products
(27 results)
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[Journal Article] Selective control of inhibitory synapse development by Slitrk3-PTPδ trans-synaptic interaction2012
Author(s)
Hideto Takahashi, Kei-ichi Katayama, Kazuhiro Sohya, Hiroyuki Miyamoto, Tuhina Prasad, Yoshifumi Matsumoto, Maya Ota, Hiroki Yasuda, Tadaharu Tsumoto, Jun Aruga, Ann Marie Craig
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Journal Title
Nature Neuroscience
Volume: 15
Issue: 3
Pages: 389-398
DOI
Related Report
Peer Reviewed
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[Presentation] REGULATORY ROLE OF DREBRIN IN HIPPOCAMPUSDEPENDENT LEARNING ISN-ESN-20112011
Author(s)
Shirao, T., Hanamura, K., Yasuda, H., Kajita, Y., Kamata, Y., Sekino, Y. and Kojima, N.
Organizer
(International Society for Neurochemistry and European Society for Neurochemistry) 23^<rd> Biennial Meeting
Place of Presentation
Athens, Greece
Year and Date
2011-08-30
Related Report
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[Presentation] REGULATORY ROLE OF DREBRIN IN HIPPOCAMPUSDEPENDENT LEARNING2011
Author(s)
Shirao, T., Hanamura, K., Yasuda, H., Kajita, Y., Kamata, Y., Sekino, Y., Kojima, N.
Organizer
ISN-ESN-2011 (International Society for Neurochemistry and European Society for Neurochemistry) 23^<rd> Biennial Meeting
Place of Presentation
Athens, Greece
Year and Date
2011-08-30
Related Report
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