Development of the mechanism of mammalian oocyte generation and novel utilization using large scale oocyte production
| Project/Area Number |
21200018
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Laboratory animal science
Applied animal science
|
|---|
| Research Institution | The Institute of Physical and Chemical Research |
|---|
Principal Investigator |
HONDA Arata 独立行政法人理化学研究所, 遺伝工学基盤技術室, 客員研究員 (10373367)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥28,990,000 (Direct Cost: ¥22,300,000、Indirect Cost: ¥6,690,000)
Fiscal Year 2011: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
Fiscal Year 2010: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2009: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
|
|---|
| Keywords | 卵子 / 発生工学 / 培養 / 生殖細胞 / マウス / 発育 / 成熟 / アポトーシス / 卵巣 / 莢膜幹細胞 / 大量調製 / 卵巣再構築 / 体外培養 / 体外発育 |
|---|
| Research Abstract |
Although neonatal mammalian ovaries contain many nongrowing primordial oocytes, most degenerate and only a few contribute to the oocyte pool in the mature ovary. If these arrested neonatal primordial oocytes could be rescued effectively, they would form a potentially valuable source for research, clinical, and zoological purposes. In this research, we developed a follicle-free culture system that enables a large number of arrested oocytes to develop in vitro. By selective culture of ovarian cells from newborn (2-4 days old) mice, spherical colonies consisting exclusively of primordial oocytes and putative thecal stem cells were obtained. Upon treatment with a stem cell factor (c-kit ligand), these embedded oocytes were released from the colonies. The released oocytes-more than 800 per animal-continued to develop without any supporting cells, formed a zona pellucida, and were able to fuse with spermatozoa. This culture system provides a unique experimental model for studying the mechanisms of oogenesis, as it can supply many granulosa cell-free oocytes at specific stages. We successfully generated MII oocytes using ectopic transplantation method that was constructed by in vitro cultured oocytes and neonatal ovarian somatic cells. Furthermore, we noticed a novel oocyte apoptosis mechanism which is controlled by oocyte autonomously.
|
Report
(4 results)
Research Products
(4 results)
-
-
[Journal Article] Large-scale production of growing oocytes in vitro from neonatal mouse ovaries2009
Author(s)
Arata Honda, Michiko Hirose, Kimiko Inoue, Hitoshi Hiura, Hiromi Miki, Narumi Ogonuki, Michihiko Sugimoto, Kuniya Abe, Mito Kanatsu-Shinohara, Tomohiro Kono, Takashi Shinohara, Atsuo Ogura
-
Journal Title
International Journal of Developmental Biology
Volume: 53
Pages: 605-613
Related Report
Peer Reviewed
-
-
