| Project/Area Number |
21200036
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
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| Allocation Type | Single-year Grants |
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| Research Field |
Virology
Nanomaterials/Nanobioscience
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| Research Institution | The University of Tokyo |
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Principal Investigator |
YONEDA Misako 東京大学, 医科学研究所, 准教授 (40361620)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIYUKI Tomoko 東京大学, 医科学研究所, 特任研究員 (50610630)
OMI Mio 東京大学, 医科学研究所, 特任研究員 (00526767)
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| Co-Investigator(Renkei-kenkyūsha) |
YASUDA Kenji 東京医科歯科大学, 生体材料工学研究所, 教授 (20313158)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥30,940,000 (Direct Cost: ¥23,800,000、Indirect Cost: ¥7,140,000)
Fiscal Year 2011: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2010: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2009: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
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| Keywords | 細胞 / 麻疹ウイルス / ニパウイルス / 膜たんぱく / アクセサリー蛋白 / 病原性 / 細胞内局在 / 個体内伝播 / 神経細胞 / AMCA培養系 |
|---|
| Research Abstract |
Both Measles and Nipah virus causes severe acute or late-onset encephalitis in human. In this study, we aimed to investigate the mechanisms of measles and nipah virus spread between neural cells. We established the AMCA (on-chip agarose microchamber array) system for primary neural cells, and elucidated the propagation manner of MV to neighboring cells. By using fluorescent labeled- viral protein, a new domain, which was important for interaction of NiV N and P protein, was identified. Further, we investigated NiV spread in African green monkey by using EGFP-expressing recombinant NiV. The results indicated that lethal infection to CNS occurred after systemic infection.
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