A novel proteomics approach for identification of protein-RNA complex associated with specific gene loci using a human artificial chromosome (HAC)
Project/Area Number |
21200038
|
Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
|
Allocation Type | Single-year Grants |
Research Field |
Applied genomics
Genetics/Genome dynamics
|
Research Institution | Tottori University |
Principal Investigator |
KUGOH Hiroyuki 鳥取大学, 大学院・医学系研究科, 准教授 (40225131)
|
Co-Investigator(Kenkyū-buntansha) |
NAKAYAMA Yuji 鳥取大学, 生命機能研究支援センター, 助教 (40432603)
|
Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥30,940,000 (Direct Cost: ¥23,800,000、Indirect Cost: ¥7,140,000)
Fiscal Year 2011: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2010: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2009: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
|
Keywords | 染色体工学 / HAC / LIT1 / CGG / Xist / ゲノム / ゲノムネットワーク / 染色体 / 免疫沈降 / RNA結合タンパク質 |
Research Abstract |
To recover the CHO chromosome that carry 3.5kb of the lacO repeat sequences in the cells, we carried out immunoprecipitation by using anti-GFP antibody after the introduction of the LacI-GFP vector. As a result, a large size of CHO chromosome was recovered by anti-GFP antibody. These results suggest that the possibility of recovery of the HAC by chromatin immunoprecipitation. In addition, our colleague has developed a new HAC vector (MI-HAC) with multi-integrase recombination sites. MI-HAC enables to introduce various large genes or genomic loci. Furthermore, we produced MI-HAC that contains the lacO repeat sequences and targeted gene loci (Xist, LIT1/KCNQ1OT1 and CGG repeat sequence).
|
Report
(4 results)
Research Products
(66 results)
-
[Journal Article] Electrophysiological properties of prion-positive cardiac progenitors derived from murine embryonic stem cells2012
Author(s)
Fujii H, Ikeuchi Y, Kurata Y, Ikeda N, Bahrudin U, Li P, Nakayama Y, Endo R, Hasegawa A, Morikawa K, Miake J, Yoshida A, Hidaka K, Morisaki T, Ninomiya H, Shirayoshi Y, Yamamoto K, Hisatome I
-
Journal Title
Circ J
Volume: 76(12)
Pages: 2875-83
NAID
Related Report
Peer Reviewed
-
[Journal Article] Down-regulation of MutS homolog 3 by hypoxia in human colorectal cancer2012
Author(s)
Li J, Koike J, Kugoh H, Arita M, Ohhira T, Kikuchi Y, Funahashi K, Takamatsu K, Boland CR, Koi M, Hemmi H
-
Journal Title
Biochim Biophys Acta -Molecular Cell Research
Volume: 1823(4)
Issue: 4
Pages: 889-899
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] SIRT2 downregulation in HeLa can induce p53 accumulation via p38 MAPK activation-dependent p300 decrease, eventually leading to apoptosis2011
Author(s)
Li YC, Matsumori H, Nakayama Y, Osaki M, Kojima H, Kurimasa A, Ito H, Mori S, Katoh M, Oshimura M, and Inoue T
-
Journal Title
Genes to Cells
Volume: 16
Pages: 34-45
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] Identification of the chromatin regions coated by non-coding Xist RNA2009
Author(s)
Murakami, K., Ohhira, T., Oshiro, E., Qi, D., Oshimura, M., Kugoh, H
-
Journal Title
Cytogenetic and Genome Research
Volume: 125(1)
Pages: 19-25
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Human artificial chromosomes for gene delivery and stable expression2012
Author(s)
M. Oshimura, Y. Kazuki, M. Hiratsuka, T. Ohbayashi, Y. Iida, N. Uno, E. Ueno, K. Ueda, T. Ohhira, H. Kurosaki, S. Abe, H. Kugoh
Place of Presentation
PepTalk, Coronado, CA, USA, HotelDel Coronado
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Evidence for a hTERT repressor gene on human chromosome 3p21.3 by using chromosome engineering2011
Author(s)
T. Ohhira, S. Abe, H. Tanaka, T. Notsu, S. Horike, D.L. Qi, C. Fujisaki, D. Gilly, M. Oshimura, H. Kugoh
Organizer
61^<th> American Society of Human Genetics (ASHG)
Place of Presentation
Montreal, Canada, Montreal Convention Centre
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Further investigation of IMMP2L2009
Author(s)
N. H. Sykes, A. T. Pagnamenta, G. Lunter, H. Kugoh, M. Oshimura, Elena Bacchelli, Elena Maestrini, A. J. Bailey, A. P. Monaco
Organizer
LRRN3 and DOCK4 as potential candidate genes for autism susceptibility
Place of Presentation
ASHG, Hawaii
Related Report
-
-
-
-
-
-