Multi-disciplinary analysis ob 50-100nt-sized,small RNAs Universally found in mouse cells
Project/Area Number |
21200039
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Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
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Allocation Type | Single-year Grants |
Research Field |
Molecular biology
ゲノム情報科学
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Research Institution | University of Tsukuba (2011) 大学共同利用機関法人情報・システム研究機構(新領域融合研究センター、DBCLS) (2010) The Institute of Physical and Chemical Research (2009) |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
KAWAI Gota 千葉工業大学, 工学部, 教授 (70211860)
USHIDA Chisato 弘前大学, 農学生命科学部, 准教授 (50250593)
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Project Period (FY) |
2009 – 2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥30,940,000 (Direct Cost: ¥23,800,000、Indirect Cost: ¥7,140,000)
Fiscal Year 2011: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2010: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2009: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
|
Keywords | RNA / アンチセンスRNA / 低分子RNA / non-coding RNA / NMR / 立体構造 / センス-アンチセンス転写産物 / SAT(センス-アンチセンス転写産物) / non-codin RNA |
Research Abstract |
We performed genome-wide analysis of novel-sized (50-100nt) small RNAs that is originally discovered in the gene loci producing endogenous antisense RNA, based on the custom-made microarrays. The expression validation was analyzed by Northern hybridization. We developed a structural screening method by the in NMR tube transcription for the structural analysis of small RNA. We also performed a trial expression analysis of small RNAs using the next generation sequencers and structural clustering of the RNA sequences.
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Report
(4 results)
Research Products
(34 results)
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[Journal Article] Comprehensive expressional analyses of antisense transcripts in colon cancer tissues using artificial antisense probes2011
Author(s)
Saito R, Kohno K, Okada Y, Osada Y, Numata K, Kohama C, Watanabe K, Nakaoka H, Yamamoto N, Kanai A, Yasue H, Murata S, Abe K, Tomita M, Ohkohchi N, and Kiyosawa H
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Journal Title
BMC Medical Genomics
Volume: 4
Pages: 42-42
Related Report
Peer Reviewed
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[Journal Article] A small nucleolar RNA functions in rRNA processing in C. elegans2010
Author(s)
Hokii Y, Sasano Y, Sato M, Sakamoto H, Sakata K, Shingai R, Taneda A, Oka S, Himeno H, Muto A, Fujiwara T, and Ushida C
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Journal Title
Nucleic Acids Res.
Volume: 38
Pages: 5909-5918
Related Report
Peer Reviewed
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