Investigating the relationship between the conformation of nuclear membrane and the functions of chromatin

• Project/Area Number: 21200040
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
• Allocation Type: Single-year Grants
• Research Field: Cell biology; Applied genomics
• Research Institution: Hosei University (2011); Tohoku University (2009-2010)
• Principal Investigator: OHBA Tomoyuki 法政大学, 企画戦略本部, 特任准教授 (80380666)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥29,510,000 (Direct Cost: ¥22,700,000、Indirect Cost: ¥6,810,000); Fiscal Year 2011: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000); Fiscal Year 2010: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000); Fiscal Year 2009: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
• Keywords: 染色体構造 / 転写因子 / シグナル伝達 / 核構造 / 染色体 / IκB-ζ

Research Abstract

In order to control the activity of signal transduction components, they were expressed as mutated hormone binding domain of human estrogen receptor fusion proteins, whose activity is regulated by the addition of a small synthetic compound, 4-hydroxytamoxifen. These components show that the signaling pathway leading to nuclear factor-kB activation and the post-transcriptional activation bifurcates at IRAK1, suggesting a new pathway activated by IRAK1.

Research Products

• [Journal Article] (2012)
  • Author(s): Tomoyuki Ohba, Yujiro Ariga, Takashi Maruyama, Nha K. Truong, Jun-ichiro Inoue, Tatsushi Muta
  • Journal Title: FEBS Journal Volume: 279(2) Pages: 211-222
  • Peer Reviewed
• [Journal Article] Identification of interleukin-1 receptor-associated kinase 1 as a critical component that induces post-transcriptional activation of IκB-ζ (2012)
  • Author(s): Ohba T, Ariga Y, Maruyama T, Truong NK, Inoue J, Muta T
  • Journal Title: The FEBS Journal Volume: 279 Issue: 2 Pages: 211-222
  • DOI: 10.1111/j.1742-4658.2011.08416.x
  • Peer Reviewed
• [Presentation] Induction of IκB-ζ by Activations of Transcription and Post-transcriptional Regulation on B Cell Receptor Stimulation (2011)
  • Author(s): Hanihara F., Ohba T., Muta T.
  • Organizer: 第34回日本分子生物学
  • Place of Presentation: 横浜 パシフィコ横浜
  • Year and Date: 2011-12-14
• [Presentation] Induction of IkB-ζ by Activations of Transcription and Post-transcriptional Regulation on B Cell Receptor Stimulation (2011)
  • Author(s): Fumito Hanihara, Tomoyuki Ohba, Tatsushi Muta
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 神奈川県横浜市パシフィコ横浜
  • Year and Date: 2011-12-14
• [Presentation] IκB-ζ-deficiency in Non-lymphoid Cells Spontaneously Induces Autoimmune Reactions (2011)
  • Author(s): Okuma A., Ohba T., Ono M., Muta T.
  • Organizer: 第40回日本免疫学会総会
  • Place of Presentation: 千葉 幕張メッセ
  • Year and Date: 2011-11-28
• [Presentation] IkB-ζ-deficiency in Non-lympoid Cells Spontaneously Induces Autoimmune Reactions (2011)
  • Author(s): OKUMA Atsushi, OHBA Tomoyuki, ONO Masao, MUTA Tatsushi
  • Organizer: 第40回日本免疫学会総会
  • Place of Presentation: 千葉県千葉市幕張メッセ
  • Year and Date: 2011-11-28
• [Presentation] Chronic Skin Inflammation Developed in Mice Lacking IκB-ζ, Crucial NF-κB Regulator (2010)
  • Author(s): Okuma A., Ohba T., Muta T.
  • Organizer: Keystone Symposia Meeting NF-κB in Inflammation and Disease
  • Place of Presentation: Santa Fe, USA
  • Year and Date: 2010-01-07
• [Presentation] Chronic Skin Inflammation Developed in Mice Lacking IκB-ζ, Crucial NF-κB Regulator (2010)
  • Author(s): 大熊敦史, 大場誠介, 牟田達史
  • Organizer: Keystone Symposium "NF-κB in Inflammation and Disease
  • Place of Presentation: サンタフェ、アメリカ
  • Year and Date: 2010-01-07
• [Presentation] Roles for Lymphocytes and Non-hematopoietic Cells in the Pathogenesis of Chronic Skin Inflammation in IκB-ζ-deficient Mice (2010)
  • Author(s): Okuma A., Ohba T., Muta T.
  • Organizer: The 11th Annual Meeting of The Society for Leukocyte Biology & The International Endotoxin and Innate Immunity Society
  • Place of Presentation: Vancouver, Canada
• [Presentation] Stimulus-specific Post-transcriptional Regulation of IκB-ζ in Innate and Adaptive Immune Responses (2010)
  • Author(s): Hanihara F., Takahashi Y., Ohba T., Muta T.
  • Organizer: The 11th annual IEIIS meeting
  • Place of Presentation: Vancouver, Canada
• [Presentation] Identification of the Toll-like receptor signaling components involved in the post-transcriptional regulation of IκB-ζ (2010)
  • Author(s): Ariga Y., Ohba T., Truong N. K., Muta T.
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: Kobe, Japan, Kobe Convention Center Complex
• [Presentation] Stimulus-specific post-transcriptional regulation of IκB-ζ, an essential regulator on inflammatory responses (2010)
  • Author(s): Hanihara F., Takahashi Y., T. Ohba T., Muta T.
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: Kobe, Japan, Kobe Convention Center Complex
• [Presentation] Chemokines induction of macrophages in response to dead cells (2010)
  • Author(s): Kimura T., Watanabe E., Hanihara F., Ohba T., Muta T.
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: Kobe, Japan, Kobe Convention Center Complex
• [Presentation] Analysis on the pathogenesis of chronic skin inflammation that spontaneously develops in IκB-ζ gene-deficient mice (2010)
  • Author(s): Okuma A., Ohba T., Ono M., Shibuya S., Aiba S., Muta T.
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: Kobe, Japan, Kobe Convention Center Complex
• [Presentation] Molecular Dissection of the MyD88-dependent Signaling that Leads to Specific mRNA Stabilization (2010)
  • Author(s): Ariga Y., Hanihara F., Ohba T., Muta T.
  • Organizer: Keystone Symposia Meeting Innate Immunity: Mechanisms Linking with Adaptive Immunity
  • Place of Presentation: Dublin, Ireland
• [Presentation] Stimulus-specific Post-transcriptional Regulation of IκB-ζ in Innate and Adaptive Immune Responses (2010)
  • Author(s): Fumito Hanihara, Yuta Takahashi, Tomoyuki Ohba, Tatsushi Muta
  • Organizer: The 11th Annual Meeting of The Society for Leukocyte Biology & The International Endotoxin and Innate Immunity Society
  • Place of Presentation: Vancouver, Canada
• [Presentation] Identification of the Toll-like receptor signaling components involved in the post-transcriptional regulation of IκB-ζ (2010)
  • Author(s): Yuziro Ariga, Tomoyuki Ohba, Truong N.K., Tatsushi Muta
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: Kobe, Japan(大場発表)
• [Presentation] Stimulus-specific post-transcriptional regulation of IκB-ζ, an essential regulator on inflammatory responses (2010)
  • Author(s): Fumito Hanihara, Yuta Takahashi, Tomoyuki Ohba, Tatsushi Muta
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: Kobe, Japan
• [Presentation] Chemokines induction of macrophages in response to dead cells (2010)
  • Author(s): Toshifumi Kimura, Emi Watanabe, Fumito Hanihara, Tomoyuki Ohba, Tatsushi Muta
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: Kobe, Japan
• [Presentation] Analysis on the pathogenesis of chronic skin inflammation that spontaneously develops in IκB-ζ gene-deficient mice (2010)
  • Author(s): Atsushi Okuma, Tomoyuki Ohba, Tatsushi Muta
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: Kobe, Japan
• [Presentation] Molecular Dissection of the MyD88-dependent Signaling that Leads to Specific mRNA Stabilization (2010)
  • Author(s): Yuziro Ariga, Fumito Hanihara, Tomoyuki Ohba, Tatsushi Muta
  • Organizer: Keystone Symposia Meeting Innate Immunity : Mechanisms Linking with Adaptive Immunity
  • Place of Presentation: Dublin, Ireland
• [Presentation] Mechanisms for stimulus-specific post-transcriptional regulation that determines induction of ＩκB-ζ, an essential regulator for selective gene expression on inflammatory responses (2009)
  • Author(s): 埴原文人、高橋優太、大場誠介、牟田達史
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜 パシフィコ横浜
  • Year and Date: 2009-12-12
• [Presentation] Mechanisms for stimulus-specific post-transcriptional regulation that determines induction of IκB-z, an essential regulator for selective gene expression on inflammatory responses (2009)
  • Author(s): 埴原文人、高橋優太、大場誠介、牟田達史
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] エストロゲン受容体との融合タンパク質を活用したToll-like Receptorシグナル伝達因子の活性操作系の確立 (2009)
  • Author(s): 有賀裕二郎、大場誠介、Nha K. Truong、牟田達史
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜 パシフィコ横浜
  • Year and Date: 2009-12-10
• [Presentation] エストロゲン受容体との融合タンパク質を活用した Toll-like Receptor シグナル伝達因子の活性操作系の確立 (2009)
  • Author(s): 有賀裕二郎、大場誠介、Nha K.Truong、牟田達史
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] IκB-ζ結合タンパク質としてのTAB2 の同定とそのIκB-ζ依存性転写活性化における関与 (2009)
  • Author(s): 大坪茂記、有賀裕二郎、大場誠介、牟田達史
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜 パシフィコ横浜
  • Year and Date: 2009-12-09
• [Presentation] IκB-ζ結合タンパク質としてのTAB2の同定とそのIκB-ζ依存性転写活性化における関与 (2009)
  • Author(s): 大坪茂記、有賀裕二郎、大場誠介、牟田達史
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-09
• [Presentation] IκB-ζ遺伝子欠損マウスにおける慢性皮膚炎の発症機序の解析 (2009)
  • Author(s): 大熊敦史、大場誠介、小野栄夫、渋谷佐和子、相場節也、牟田達史
  • Organizer: 第39回日本免疫学会総会
  • Place of Presentation: 大阪 大阪国際会議場
  • Year and Date: 2009-12-04
• [Presentation] IκB-ζ遺伝子欠損マウスにおける慢性皮膚炎の発症機序の解析 (2009)
  • Author(s): 大熊敦史、大場誠介、小野栄夫、渋谷佐和子、相場節也、牟田達史
  • Organizer: 第39回日本免疫学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-04
• [Presentation] Mechanism for Stimulus-specific Post-transcriptional Regulation of IκB-ζ, which Controls Selective Gene Expression in Inflammatory Responses (2009)
  • Author(s): 埴原文人、高橋優太、大場誠介、牟田達史
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸 神戸国際会議場
  • Year and Date: 2009-10-23
• [Presentation] Mechanism for Stimulus-specific Post-transcriptional Regulation of I□B-□, which Controls Selective Gene Expression in Inflammatory Responses (2009)
  • Author(s): 埴原文人、高橋優太、大場誠介、牟田達史
  • Organizer: 第82回日本生化学会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-23
• [Presentation] IκB-ζ遺伝子欠損マウスにおける慢性皮膚炎の発症機序の解析 (2009)
  • Author(s): 大熊敦史、大場誠介、小野栄夫、渋谷佐和子、相場節也、牟田達史
  • Organizer: 第6回東北大学バイオサイエンスシンポジウム
  • Place of Presentation: 仙台
  • Year and Date: 2009-06-16