Photoresponsive RNA-binding ligands for regulation of RNA functions

• Project/Area Number: 21200042
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
• Allocation Type: Single-year Grants
• Research Field: Living organism molecular science; Chemistry related to living body
• Research Institution: Osaka University
• Principal Investigator: DHONO Chikara 大阪大学, 産業科学研究所, 准教授 (60420395)
• Co-Investigator(Renkei-kenkyūsha): NAKATANI Kazuhiko 大阪大学, 産業科学研究所, 教授 (70237303)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥30,810,000 (Direct Cost: ¥23,700,000、Indirect Cost: ¥7,110,000); Fiscal Year 2011: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000); Fiscal Year 2010: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000); Fiscal Year 2009: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
• Keywords: 分子認識 / ケミカルバイオロジー / バイオテクノロジー / RNA / DNA / ミスマッチ / 分子糊 / 光スイッチ / 生体認識・機能化学 / RNA結合リガンド

Research Abstract

Recent studies have revealed that RNA molecules directly involve in modulation of a wide variety of cellular functions. We have developed a series of small synthetic ligands that can bind to specific RNA structures and regulate the RNA-related functions. Ligand binding induced the structural change of target mRNA and resulted in translational regulation of the gene expression through a riboswitch-type regulatory mechanism.

Research Products

• [Journal Article] Naphthyridine tetramer with a preorganized structure for 1:1 binding to a CGG/CGG sequence (2012)
  • Author(s): Dohno, C.; Kohyama, I.; Hong, C; Nakatani, K.
  • Journal Title: Nucleic.Acids Res. Volume: 40 Issue: 6 Pages: 2771-2781
  • DOI: 10.1093/nar/gkr1148
  • Peer Reviewed
• [Journal Article] Naphthyridine tetramer with a preorganized structure for 1:1 binding to a CGG/CGG sequence (2011)
  • Author(s): Dohno, C.; Kohyama, I.; Hong, C.; Nakatani, K.
  • Journal Title: Nucleic. Acids. Res. Volume: 40 Pages: 2771-2781
  • Peer Reviewed
• [Journal Article] Control of DNA hybridization by photoswitchable molecular glue (2011)
  • Author(s): Dohno, C.; Nakatani, K.
  • Journal Title: Chem. Soc. Rev. Volume: 40 Pages: 5718-5729
  • Peer Reviewed
• [Journal Article] Molecular-Glue-Triggered DNA Assembly To Form a Robust and Photoresponsive Nano-Network (2011)
  • Author(s): Wang, C.; Pu, F.; Lin, Y.; Ren, J.; Dohno, C.; Nakatani K.; Qu, X.
  • Journal Title: Chem. Eur. J. Volume: 17 Pages: 8189-8197
  • Peer Reviewed
• [Journal Article] Ligand Inducible Assembly of a DNA Tetrahedron (2011)
  • Author(s): Dohno, C.; Atsumi, H.; Nakatani, K.
  • Journal Title: Chem. Commun. Volume: 47 Pages: 3499-3501
  • Peer Reviewed
• [Journal Article] Control of DNA hybridization by photoswitchable molecular glue (2011)
  • Author(s): Dohno, C.; Nakatani, K.
  • Journal Title: Chem.Soc.Rev. Volume: 40 Issue: 12 Pages: 5718-5729
  • DOI: 10.1039/c1cs15062f
  • Peer Reviewed
• [Journal Article] Molecular-Glue-Triggered DNA Assembly to Form a Robust and Photore sponsive Nano-Network (2011)
  • Author(s): Wang, C.; Pu, F.; Lin, Y.; Ren, J.; Dohno, C.; Nakatani K.; Qu, X.
  • Journal Title: Chem.Eur.J. Volume: 17 Issue: 29 Pages: 8189-8197
  • DOI: 10.1002/chem.201100194
  • Peer Reviewed
• [Journal Article] Ligand Inducible Assembly of a DNA Tetrahedron (2011)
  • Author(s): Dohno, C., et al.
  • Journal Title: Chem.Commun. Volume: 47 Pages: 3499-3501
  • Peer Reviewed
• [Journal Article] Discrimination of N6-methyl Adenine in a Specific DNA Sequence (2010)
  • Author(s): Dohno, C., et al.
  • Journal Title: Chem.Commun. Volume: 46 Pages: 5530-5532
  • Peer Reviewed
• [Journal Article] A Light-Driven, Supramolecular Optical Switch (2009)
  • Author(s): Uno, S.; Dohno, C.; Bittermann, H.; Malinovskii, V. L.; Haner, R.; Nakatani, K.
  • Journal Title: Angew. Chem. Int. Ed. Volume: 48 Pages: 7362-7365
  • Peer Reviewed
• [Journal Article] Photoswitchable Unsymmetrical Ligand for DNA Hetero-Mismatches (2009)
  • Author(s): Dohno, C.; Yamamoto, T.; Nakatani, K.
  • Journal Title: Eur. J. Org. Chem. Pages: 4051-4058
  • Peer Reviewed
• [Journal Article] The Effect of Linker Length on Binding Affinity of a Photoswitchable Molecular Glue for DNA (2009)
  • Author(s): Dohno, C.; Uno, S.; Sakai, S.; Oku, M.; Nakatani, K.
  • Journal Title: Bioorg. Med. Chem. Volume: 17 Pages: 2536-2543
  • Peer Reviewed
• [Journal Article] The effect of linker length on binding affinity of a photoswitchable molecular glue for DNA (2009)
  • Author(s): C.Dohno, et.al.
  • Journal Title: Bioorg.Med.Chem. Volume: 17 Pages: 2536-2543
  • Peer Reviewed
• [Journal Article] Photoswitchable Unsymmetrical Ligand for DNA Hetero-Mismatches (2009)
  • Author(s): C.Dohno, et.al.
  • Journal Title: Eur.J.Org.Chem. Pages: 4051-4058
  • Peer Reviewed
• [Journal Article] A Light-Driven, Supramolecular Optical Switch (2009)
  • Author(s): S.Uno, et.al.
  • Journal Title: Angew.Chem.Int.Ed. Volume: 48 Pages: 7362-7365
  • Peer Reviewed
• [Presentation] Design and synthesis of RNA binding ligand for regulating gene expression (2012)
  • Author(s): Dohno, C. (代表)
  • Organizer: 243rd American Chemical Society National Meeting
  • Place of Presentation: サンディエゴ(米国)
  • Year and Date: 2012-03-27
• [Presentation] Design and synthesis of RNA binding ligand for regulating gene expression (2012)
  • Author(s): Chikara Dohno, Izumi Kohyama, Kazuhiko Nakatani
  • Organizer: 243rd American Chemical Society National Meeting
  • Place of Presentation: San Diego (USA)
  • Year and Date: 2012-03-27
• [Presentation] Naphthyridine tetramer functions as a molecular glue for DNA and RNA (2011)
  • Author(s): Dohno, C. (代表)
  • Organizer: 第38回国際核酸化学シンポジウム
  • Place of Presentation: 北海道大学(札幌)
  • Year and Date: 2011-11-09
• [Presentation] Photoswitchable molecular glue for hybridization of nucleic acids (2011)
  • Author(s): Dohno, C. (代表)
  • Organizer: XVth Symposium on Chemistry of Nucleic Acid Components
  • Place of Presentation: チェスキークルムロフ(チェコ)
  • Year and Date: 2011-06-07
• [Presentation] Photoswitchable molecular glue for DNA Nanotechnology (2011)
  • Author(s): Dohno, C. (代表)
  • Organizer: International Symposium: Advanced Science and Technology for Single Molecular Analysis of DNA and Related Molecules (ISSMA2011)
  • Place of Presentation: 京都国際会議場(京都)(招待講演)
  • Year and Date: 2011-01-25
• [Presentation] Photoswitchable molecular glue for DNA Nanotechnology (2011)
  • Author(s): Chikara Dohno
  • Organizer: International Symposium : Advanced Science and Technology for Single Molecular Analysis of DNA and Related Molecules (ISSMA2011)
  • Place of Presentation: 京都国際会議場(京都)(招待講演)
  • Year and Date: 2011-01-25
• [Presentation] Photoswitching of DNA hybridization by a small synthetic ligand (2010)
  • Author(s): Dohno, C. (代表)
  • Organizer: The International Chemical Congress of Pacific Basin Societies (Pacifichem2010)
  • Place of Presentation: ホノルル(米国)
  • Year and Date: 2010-12-17
• [Presentation] Recognition of nucleic acids by tetrameric naphthyridine derivatives (2010)
  • Author(s): Kohyama, I
  • Organizer: The International Chemical Congress of Pacific Basin Societies (Pacifichem2010)
  • Place of Presentation: ホノルル(米国)
  • Year and Date: 2010-12-17
• [Presentation] Binding of tetrameric naphthyridine derivatives to DNA containing a GG-mismatch (2010)
  • Author(s): Dohno, C. (代表)
  • Organizer: 第37回国際核酸化学シンポジウム
  • Place of Presentation: はまぎんホール(横浜)
  • Year and Date: 2010-11-11
• [Presentation] 光応答性分子糊を用いたDNA光スイッチングデバイス (2010)
  • Author(s): 堂野主税(代表)
  • Organizer: 日本化学会第90春季年会
  • Place of Presentation: 近畿大学(大阪府)
  • Year and Date: 2010-03-27
• [Presentation] Binding of tetrameric naphthyridine derivatives to DNA containing a GG-mismatch (2010)
  • Author(s): Izumi Kohyama
  • Organizer: 第37回国際核酸化学シンポジウム(ISNAC2010)
  • Place of Presentation: はまぎんホール(横浜)
• [Presentation] 核酸二次構造安定性を大きく変化させる新規光応答性小分子リガンド (2009)
  • Author(s): 堂野主税(代表)
  • Organizer: 第24回生体機能関連化学シンポジウム
  • Place of Presentation: 九州大学(福岡県)(招待講演)
  • Year and Date: 2009-09-13
• [Presentation] 核酸二次構造安定性を大きく変化させる新規光応答性小分子リガンド (2009)
  • Author(s): 堂野主税
  • Organizer: 第24回生体機能関連化学シンポジウム
  • Place of Presentation: 九州大学(福岡県)
  • Year and Date: 2009-09-13