| Budget Amount *help |
¥30,940,000 (Direct Cost: ¥23,800,000、Indirect Cost: ¥7,140,000)
Fiscal Year 2011: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2010: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2009: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
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| Research Abstract |
The aim of this study is to reconstitute homologous recombination in vitro, which can be used to produce hybrid genes and proteins. In this study, all proteins which participate in homologous recombination were prepared. Using these proteins, each step of homologous recombination was reconstituted in vitro. After optimization of each step, whole homologous recombination system was successfully reconstituted in vitro.
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