A novel technique producing hybrid proteins by using homologous recombination in vitro
| Project/Area Number |
21200058
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied biochemistry
Chemistry related to living body
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
MIKAWA Tsutomu 独立行政法人理化学研究所, 遺伝制御科学特別研究ユニット, 専任研究員 (20321820)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥30,940,000 (Direct Cost: ¥23,800,000、Indirect Cost: ¥7,140,000)
Fiscal Year 2011: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2010: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2009: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
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| Keywords | バイオテクノロジー / タンパク質工学 / タンパク質 / 蛋白質 / 蛋白質工学 |
|---|
| Research Abstract |
The aim of this study is to reconstitute homologous recombination in vitro, which can be used to produce hybrid genes and proteins. In this study, all proteins which participate in homologous recombination were prepared. Using these proteins, each step of homologous recombination was reconstituted in vitro. After optimization of each step, whole homologous recombination system was successfully reconstituted in vitro.
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Report
(4 results)
Research Products
(95 results)
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[Journal Article] The double-stranded break-forming activity of plant SPO11s and a novel rice SPO11 revealed by a Drosophila bioassay2012
Author(s)
Yoshinori Shingu, Takeshi Tokai, Yasuo Agawa, Kentaro Toyota, Selina Ahamed, Makiko Kawagishi-Kobayashi, Akira Komatsu, Tsutomu Mikawa, Masa-Toshi Yamamoto, Kyo Wakasa, Takehiko Shibata, Kohji Kusano
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Journal Title
BMC molecular biology
Volume: vol.13
Pages: 1-1
Related Report
Peer Reviewed
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[Journal Article] NMR protein structure determination in living E. coli cells using non-linear sampling2011
Author(s)
Teppei Ikeya, Atsuko Sasaki, Daisuke Sakakibara, Yoshiki Shigemitsu, Junpei Hamatsu, Tomomi Hanashima, Masaki Mishima, Masatoshi Yoshimasu, Nobuhiro Hayashi, Tsutomu Mikawa, Daniel Nietlispach, Markus Walchli, Brian O. Smith, Masahiro Shirakawa, Peter Guntert, Yutaka Ito
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Journal Title
Nature Protocols
Volume: Vol.5, No.6
Pages: 1051-1060
Related Report
Peer Reviewed
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[Journal Article] DIDS, a chemical compound that inhibits RAD51-mediated homologous pairing and strand exchange2009
Author(s)
Takako Ishida, Yoshimasa Takizawa, Takashi Kainuma, Jin Inoue, Tsutomu Mikawa, Takehiko Shibata, Hidekazu Suzuki, Satoshi Tashiro, Hitoshi Kurumizaka
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Journal Title
Nucleic Acids Research
Volume: Vol.37, No.10
Pages: 3367-3376
Related Report
Peer Reviewed
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