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Quantitative characterization and molecular mechanism of endoplasmic reticulum dynamics

Research Project

Project/Area Number 21200065
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)

Allocation TypeSingle-year Grants
Research Field Plant molecular biology/Plant physiology
Cell biology
Research InstitutionKyoto University

Principal Investigator

UEDA Haruko  京都大学, 大学院・理学研究科, 特定准教授 (90402776)

Co-Investigator(Kenkyū-buntansha) YOKOTA Etsuo  兵庫県立大学, 大学院・生命理学研究科, 助教 (80212299)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥30,940,000 (Direct Cost: ¥23,800,000、Indirect Cost: ¥7,140,000)
Fiscal Year 2011: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2010: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2009: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
Keywords原形質流動 / 小胞体 / アクチン / ミオシン / オルガネラ
Research Abstract

Plants exhibit an ultimate case of the intracellular motility involving rapid organelle trafficking and continuous streaming of the endoplasmic reticulum (ER). To comprehensively analyze the ER movement, we developed software to generate a detailed velocity-distribution map. Streaming pattern of cytosolic GFP was similar to that of the ER-localized GFP, suggesting that a correlation and a causative relationship between the streaming of ER and cytosol. Furthermore, we elucidated a part of the molecular mechanisms of the ER streaming by analyzing ER-associated proteins responsible for ER streaming.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (32 results)

All 2012 2011 2010 2009 Other

All Journal Article (14 results) (of which Peer Reviewed: 14 results) Presentation (16 results) Remarks (2 results)

  • [Journal Article] Myosin XI-dependent formation of tubular structures from endoplasmic reticulum isolated from tobacco cultured BY-2 cells2011

    • Author(s)
      Yokota, E., Ueda, H., Hashimoto, K., Orii, H., Shimada, T., Hara-Nishimura, I., and Shimmen, T.
    • Journal Title

      Plant Physiol.

      Volume: 156 Pages: 129-143

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The myosin ATPase inhibitor, 2,3-butanedione 2-monoxime, prevents protein secretion by the basidiomycete Coprinopsis cinerea2011

    • Author(s)
      Hashimoto, K., Yokota, E., Shimmen, T., and Yoshida, M.
    • Journal Title

      Biotechnol. Lett.

      Volume: 33 Issue: 4 Pages: 769-775

    • DOI

      10.1007/s10529-010-0497-0

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Myosin XI-Dependent Formation of Tubular Structures from Endoplasmic Reticulum Isolated from Tobacco Cultured Cells, BY-2.2011

    • Author(s)
      Yokota E., et al
    • Journal Title

      Plant Physiol.

      Volume: (in press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis Qa-SNARE SYP2 proteins localized to different subcellular regions function redundantly in vacuolar protein sorting and plant development2010

    • Author(s)
      Shirakawa, M., Ueda, H., Shimada, T., Koumoto, Y., Shimada, T. L., Kondo, M., Takahashi, T., Okuyama, Y., Nishimura, M., and Hara-Nishimura, I.
    • Journal Title

      Plant J.

      Volume: 64 Issue: 6 Pages: 924-935

    • DOI

      10.1111/j.1365-313x.2010.04394.x

    • NAID

      130006994888

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Myosin-dependent endoplasmic reticulum motility and F-actin organization in plant cells2010

    • Author(s)
      Ueda, H., Yokota, E., Kutsuna, N., Shimada, T., Tamura, K., Shimmen, T., Hasezawa, S., Dolja, V. V., and Hara-Nishimura, I.
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 107 Issue: 15 Pages: 6894-6899

    • DOI

      10.1073/pnas.0911482107

    • NAID

      120002511346

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis Qa-SNARE SYP2 proteins localized to different subcellular regions function redundantly in vacuolar protein sorting and plant development.2010

    • Author(s)
      Shirakawa M., Ueda H., et al
    • Journal Title

      Plant J.

      Volume: 64 Pages: 924-935

    • NAID

      130006994888

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Myosin-dependent endoplasmic reticulum motility and F-actin organization in plant cells.2010

    • Author(s)
      Ueda, H., et al.
    • Journal Title

      Proc.Natl.Acad.Sci.U.S.A. 107

      Pages: 6894-6899

    • NAID

      120002511346

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Vacuolar SNAREs function in the formation of the leaf vascular network by regulating auxin distribution2009

    • Author(s)
      Shirakawa, M.*, Ueda, H.*, Shimada, T., Nishiyama, C., and Hara-Nishimura, I.
    • Journal Title

      Plant Cell Physiol

      Volume: 50 Issue: 7 Pages: 1319-1328

    • DOI

      10.1093/pcp/pcp076

    • NAID

      10027341930

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] GNOM-LIKE1/ERMO1 and SEC24a/ERMO2 are required for maintenance of endoplasmic reticulum morphology in Arabidopsis thaliana2009

    • Author(s)
      Nakano, R. T., Matsushima, R., Ueda, H., Tamura, K., Shimada, T., Li, L., Hayashi, Y., Kondo, M., Nishimura, M., and Hara-Nishimura, I.
    • Journal Title

      Plant Cell

      Volume: 21 Issue: 11 Pages: 3672-3685

    • DOI

      10.1105/tpc.109.068270

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The putative RNA-processing protein, THO2, is a microtubule-associated protein in tobacco2009

    • Author(s)
      Hamada, T., Igarashi, H., Taguchi, R., Fujiwara, M., Fukao, Y., Shimmen, T., Yokota, E., and Sonobe. S.
    • Journal Title

      Plant Cell Physiol

      Volume: 50 Issue: 4 Pages: 801-811

    • DOI

      10.1093/pcp/pcp024

    • NAID

      10027339590

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] An isoform of myosin XI is responsible for the translocation of endoplasmic reticulum in tobacco cultured BY-2 cells2009

    • Author(s)
      Yokota, E., Ueda, S., Tamura, K., Orii, H., Uchi, S., Sonobe, S., Hara-Nishimura, I., and Shimmen, T.
    • Journal Title

      J. Exp. Bot.

      Volume: 60 Pages: 197-212

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The preprophase band is a localized center of clathrin-mediated endocytosis in late prophase cells of the onion cotyledon epidermis2009

    • Author(s)
      Karahara, I., Suda, J., Tahara, H., Yokota, E., Shimmen, T., Misaki, K., Yonemura, S., Murata, T., Staehelin, L. A., and Mineyuki, Y.
    • Journal Title

      Plant J.

      Volume: 57 Pages: 819-831

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Vacuolar SNARES function in the formation of the leaf vascular network by regulating auxin distribution.2009

    • Author(s)
      Shirakawa, M., Ueda, H., et al.
    • Journal Title

      Plant Cell Phvsiol. 50

      Pages: 1319-1328

    • NAID

      10027341930

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] GNOM-LIKE1/ERMO1 and SEC24a/ERMO2 are required for maintenance of endoplasmic reticulum morphology in Arabidopsis thaliana.2009

    • Author(s)
      Nakano, R.T., et al.
    • Journal Title

      Plant Cell 21

      Pages: 3672-3685

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 植物細胞における小胞体ダイナミクス2012

    • Author(s)
      上田晴子・他
    • Organizer
      第14回オルガネラワークショップ
    • Place of Presentation
      京都大学
    • Year and Date
      2012-03-15
    • Related Report
      2011 Final Research Report
  • [Presentation] 植物細胞における小胞体ダイナミクス2012

    • Author(s)
      上田晴子
    • Organizer
      第14回オルガネラワークショップ
    • Place of Presentation
      京都大学(京都)(招待講演)
    • Year and Date
      2012-03-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] 植物細胞におけるミオシン依存的な小胞体流動とアクチン繊維束の組織化2011

    • Author(s)
      上田晴子・他
    • Organizer
      第84回日本生化学会大会(シンポジウム : アクチンとミオシンの意外なしくみとはたらき)
    • Place of Presentation
      京都国際会館
    • Year and Date
      2011-09-21
    • Related Report
      2011 Final Research Report
  • [Presentation] 植物細胞におけるミオシン依存的な小胞体流動とアクチン繊維束の組織化2011

    • Author(s)
      上田晴子
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都国際会館(京都)(招待講演)
    • Year and Date
      2011-09-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] Myosin XI drives endoplasmic reticulum motility and organizes F-actin orientations2011

    • Author(s)
      Ueda, H. et al.
    • Organizer
      18^<th> International Botanical Congress (Symposium: Cellular Dynamics)
    • Place of Presentation
      Melbourne, Australia
    • Year and Date
      2011-07-26
    • Related Report
      2011 Final Research Report
  • [Presentation] Myosin XI drives endoplasmic reticulum motility and organizes F-actin orientations2011

    • Author(s)
      Haruko Ueda
    • Organizer
      18th International Botanical Congress
    • Place of Presentation
      Melbourne, Australia(招待講演)
    • Year and Date
      2011-07-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] 小胞体流動に異常を示す変異体の解析2011

    • Author(s)
      上田晴子・他
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      東北大学
    • Related Report
      2011 Final Research Report
  • [Presentation] 植物細胞における小胞体流動メカニズムとその意義2010

    • Author(s)
      上田晴子・他
    • Organizer
      日本植物学会近畿支部大会
    • Place of Presentation
      京都大学
    • Year and Date
      2010-11-20
    • Related Report
      2011 Final Research Report
  • [Presentation] 植物細胞における小胞体流動メカニズムとその意義2010

    • Author(s)
      上田晴子・他
    • Organizer
      第74回日本植物学会大会(シンポジウム : 画像情報から植物を定量的に捉える)
    • Place of Presentation
      中部大学(春日井市)
    • Year and Date
      2010-09-09
    • Related Report
      2011 Final Research Report
  • [Presentation] 植物細胞における小胞体流動~定量解析と生理学的意義~2010

    • Author(s)
      上田晴子
    • Organizer
      日本植物学会第74回大会
    • Place of Presentation
      中部大学
    • Year and Date
      2010-09-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] 植物細胞における小胞体流動~定量解析と生理学的意義~2010

    • Author(s)
      上田晴子・他
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本大学
    • Year and Date
      2010-03-21
    • Related Report
      2011 Final Research Report
  • [Presentation] アクチン繊維束の細胞内配向におけるミオシンXIの役割2010

    • Author(s)
      上田晴子, 横田悦雄, 朽名夏麿, 田村謙太郎, 嶋田知生, 新免輝男, 馳澤盛一郎, 西村いくこ
    • Organizer
      第51回 日本植物生理学会
    • Place of Presentation
      熊本大学
    • Year and Date
      2010-03-21
    • Related Report
      2009 Annual Research Report
  • [Presentation] ミオシンは光と重力に対する環境応答のブレーキとして働く2010

    • Author(s)
      岡本圭史, 上田晴子, 田村謙太郎, 嶋田知生, 豊田正嗣, 鈴木友美, 長谷あきら, 田坂昌生, 森田(寺尾)美代, 西村いくこ
    • Organizer
      第51回 日本植物生理学会
    • Place of Presentation
      熊本大学
    • Year and Date
      2010-03-21
    • Related Report
      2009 Annual Research Report
  • [Presentation] ER motility and F-actin organization mediated by plant-specific myosins2010

    • Author(s)
      Ueda, H. et al.
    • Organizer
      The 2010 Cold Spring Harbor Asia Conference
    • Place of Presentation
      Suzhou, China
    • Related Report
      2011 Final Research Report
  • [Presentation] ER motility and F-actin organization mediated by plant-specific myosins2010

    • Author(s)
      Ueda, H. et al.
    • Organizer
      21st International Conference on Arabidopsis Research
    • Place of Presentation
      Yokohama, Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] ER motility and F-actin organization mediated by plant-specific myosins2010

    • Author(s)
      Haruko Ueda
    • Organizer
      21st International Conference on Arabidopsis Research
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.kyoto-u.ac.jp/ja/news_data/h/h1/news6/2009/100323_1.htm

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.kyoto-u.ac.jp/ja/news_data/h/h1/news6/2009/100323_1.htm

    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2018-03-28  

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