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Establishment of a new paradigm in the phosphoinositide research

Research Project

Project/Area Number 21200075
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research a proposed research project)

Allocation TypeSingle-year Grants
Research Field Pathological medical chemistry
Biological pharmacy
Research InstitutionAkita University

Principal Investigator

SASAKI Junko  秋田大学, 大学院・医学系研究科, 講師 (30333371)

Co-Investigator(Kenkyū-buntansha) INOUE Takao  国立医薬品食品研究所, 主任研究官 (50361605)
Co-Investigator(Renkei-kenkyūsha) TAGUCHI Ryo  中部大学, 生命健康科学部, 教授 (20080210)
KOFUJI Satoshi  秋田大学, 大学院・医学系研究科, 助教 (00508153)
TAKASUGA Shunsuke  秋田大学, 大学院・医学系研究科, 助教 (90375262)
EGUCHI Satoshi  秋田大学, 大学院・医学系研究科, 助教 (70457117)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥28,730,000 (Direct Cost: ¥22,100,000、Indirect Cost: ¥6,630,000)
Fiscal Year 2011: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2010: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Fiscal Year 2009: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
Keywords分子病態学 / シグナル伝達 / 脂質 / 細胞・組織
Research Abstract

There are two isozymes (L-PIPase and P-PIPase) which dephosphorylate PI(3,4)P2. However the functions of L-PIPase and P-PIPase are unclear. In this study we showed that L-PIPase suppresses neuronal cell death after brain ischemia while P-PIPase contributes to malignant progression. We also found that each enzyme has different activities against several PI(3,4)P2 species. It is suggested that the different functions between L-PIPase and P-PIPase might be caused by the substrate specificity.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (36 results)

All 2011 2010 2009 Other

All Journal Article (25 results) (of which Peer Reviewed: 24 results) Presentation (4 results) Book (3 results) Remarks (4 results)

  • [Journal Article] Blockade of class IB phosphoinositide-3 kinase ameliorates obesity-induced inflammation and insulin resistance2011

    • Author(s)
      Kobayashi N, Ueki K, Okazaki Y, Iwane A, Kubota N, Ohsugi M, Awazawa M, Kobayashi M, Sasako T, Kaneko K, Suzuki M, Nishikawa Y, Hara K, Yoshimura K, Koshima I, Goyama s, Murakami K, Sasaki J, Nagai R, Kurokawa M, Sasaki T, Kadowaki T
    • Journal Title

      Proc.Natl.Acad.Sci.USA

      Volume: 108 Issue: 14 Pages: 5753-5758

    • DOI

      10.1073/pnas.1016430108

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Intracellular phosphatidylserine is essential for retrograde membrane traffic through endosomes2011

    • Author(s)
      Uchida, Y., Hasegawa, J., et al(22名中4番目)
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 108 Issue: 38 Pages: 15846

    • DOI

      10.1073/pnas.1109101108

    • NAID

      10030581253

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Pas-PI3K signaling pathway is involved in clathrin-independent endocytosis and the internalization of influenza viruses2011

    • Author(s)
      Fujioka, Y., Tsuda, M., et al(7名中4番目)
    • Journal Title

      PLoS One

      Volume: 6 Issue: 1 Pages: e16324-e16324

    • DOI

      10.1371/journal.pone.0016324

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Epimorphin protects hepatocytes from oxidative stress by inhibiting mitochondrial injury2011

    • Author(s)
      Kinoshita, N., Horie, Y., et al(11名中8番目)
    • Journal Title

      J. Gastroenterol Hepatol.

      Volume: 26 Issue: 1 Pages: 201-206

    • DOI

      10.1111/j.1440-1746.2010.06327.x

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Phosphoinositide 3-kinaseγ controls the intracellular localization of CpG to limit DNA-PKcs-dependent IL-10 production in macrophages2011

    • Author(s)
      Hazeki, K., Kametani, Y., et al, (11名中7番目)
    • Journal Title

      PLoS One.

      Volume: 6 Issue: 10 Pages: e26836-e26836

    • DOI

      10.1371/journal.pone.0026836

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Ras-PI3K signaling pathway is involved in clathrin-independent endocytosis and the internalization of influenza viruses.2011

    • Author(s)
      Fujioka, Y.
    • Journal Title

      PLoS One

      Volume: 6

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The PI(3,4)P2 phosphatase INPP4A is a suppressor of excitotoxic Neuronal Death2010

    • Author(s)
      Sasaki, J., Kofuji, S., et al(20名中1番目, 2番目, 9番目, 10番目)
    • Journal Title

      Nature

      Volume: 465 Issue: 7297 Pages: 497-501

    • DOI

      10.1038/nature09023

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Intracellular phospholipase A<sub>1</sub> and acyltransferase, which are involved in Caenorhabditis elegans stem cell divisions, determine the sn-1 fatty acyl chain of phosphatidylinositol2010

    • Author(s)
      Imae, R., Inoue, T., et al(8名中2番目)
    • Journal Title

      Mol. Biol. Cell

      Volume: 21 Issue: 18 Pages: 3114

    • DOI

      10.1091/mbc.e10-03-0195

    • URL

      https://pure.teikyo.jp/en/publications/57aa94b2-ab45-4806-8061-52a5ee7f657d

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Phosphatidylinositol-4-pho5-kinases and phosphatidylinositol 4,5-bisphosphate synthesis in the brain2010

    • Author(s)
      Volpicelli-Daley, L., A., Lucast, L., et al(9名中5番目)
    • Journal Title

      J. Biol. Chem.

      Volume: 285 Issue: 37 Pages: 28708-28714

    • DOI

      10.1074/jbc.m110.132191

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Decrease in membrane phospholipid unsaturation induces unfolded protein response2010

    • Author(s)
      Ariyama, H., Kono, N., et al(5名中4番目)
    • Journal Title

      J. Biol. Chem.

      Volume: 285 Issue: 29 Pages: 22027-22035

    • DOI

      10.1074/jbc.m110.126870

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Multivesicular body formation requires OSBP-related proteins and cholesterol2010

    • Author(s)
      Kobuna, H., Inoue, T., et al(7名中2番目)
    • Journal Title

      PLoS Genetics

      Volume: 6 Issue: 8 Pages: e1001055-e1001055

    • DOI

      10.1371/journal.pgen.1001055

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] LC3, a microtubule-associated protein1A/B light chain3, is involved in cytoplasmic lipid droplet formation2010

    • Author(s)
      Shibata, M., Yoshimura, K., et al(11名中6番目)
    • Journal Title

      Biochem. Biophys. Res. Commun.

      Volume: 393 Issue: 2 Pages: 274

    • DOI

      10.1016/j.bbrc.2010.01.121

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Two very long chain fatty acid acyl-CoA synthetase genes, acs-20 and acs-22, have roles in the cuticle surface barrier in Caenorhabditis elegans2010

    • Author(s)
      Kage-Nakadai, E., Kobuna, H., et al(7名中5番目)
    • Journal Title

      PLoS ONE

      Volume: 5 Issue: 1 Pages: e8857-e8857

    • DOI

      10.1371/journal.pone.0008857

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The PI(3,4)P2 phosphatase INPP4A is a suppressor of excitotoxic Neuronal Death.2010

    • Author(s)
      Sasaki, J.
    • Journal Title

      Nature

      Volume: 465 Pages: 497-501

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phosphatidylinositol-4-phosphate 5-kinases and phosphatidylinositol 4,5-bisphosphate synthesis in the brain.2010

    • Author(s)
      Volpicelli-Daley, L.A.
    • Journal Title

      J.Biol.Chem.

      Volume: 285 Pages: 28708-28714

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sex difference in the liver of hepatocyte-specific Pten-deficient mice : A model of nonalcoholic steatohepatitis2009

    • Author(s)
      Anezaki, Y., Ohshima, S., et al(14名中10番目)
    • Journal Title

      Hepatol. Res.

      Volume: 39 Issue: 6 Pages: 609-618

    • DOI

      10.1111/j.1872-034x.2009.00494.x

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Eicosapentaenoic acid ameliorates steatohepatitis and hepatocellular carcinoma in hepatocyte-specific Pten-deficient mice2009

    • Author(s)
      Ishii, H., Horie, Y., et al(14名中10番目)
    • Journal Title

      J. Hepatol.

      Volume: 50 Issue: 3 Pages: 562-571

    • DOI

      10.1016/j.jhep.2008.10.031

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mammalian phosphoinositide kinases and phosphatases2009

    • Author(s)
      Sasaki, T., Takasuga, S., Sasaki, J., Kofuji, S., Eguchi, S., Yamazaki, M. & Suzuki, A.
    • Journal Title

      Prog. Lipid Res.

      Volume: 48 Issue: 6 Pages: 307-343

    • DOI

      10.1016/j.plipres.2009.06.001

    • Related Report
      2011 Final Research Report
  • [Journal Article] Sequential regulation of DOCK2 dynamics by two phospholipids during neutrophil chemotaxis2009

    • Author(s)
      Fukui Y(著者16名、16番目)
    • Journal Title

      Science

      Volume: 324 Issue: 5925 Pages: 384-387

    • DOI

      10.1126/science.1170179

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional analysis of GS28, an intra-Golgi SNARE, in Caenorhabditis elegans2009

    • Author(s)
      Maekawa, M., Inoue, T., et al(7名中2番目)
    • Journal Title

      Genes to Cells

      Volume: 14 Issue: 8 Pages: 1003

    • DOI

      10.1111/j.1365-2443.2009.01325.x

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Normal formation of a subset of intestinal granules in Caenorhabditis elegans requires ATP-binding cassette transporters HAF-4 and HAF-9, which are highly homologous to human lysosomal peptide transporter TAP-like2009

    • Author(s)
      Kawai, H., Tanji, T., et al(13名中6番目)
    • Journal Title

      Mol. Biol. Cell

      Volume: 20 Issue: 12 Pages: 2979

    • DOI

      10.1091/mbc.e08-09-0912

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nematode homologue of PQBP1, a mental retardation causative gene, is involved in lipid metabolism2009

    • Author(s)
      Takahashi, K., Yoshina, S., et al(9名中5番目)
    • Journal Title

      PLoS ONE

      Volume: 4 Issue: 1 Pages: e4104-e4104

    • DOI

      10.1371/journal.pone.0004104

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mammalian phosphoinositide kinases and phosphatases2009

    • Author(s)
      Sasaki, T.
    • Journal Title

      Prog.Lipid Res. 48

      Pages: 307-343

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sex difference in the liver of hepatocyte-specific Pten-deficient mice : A model of nonalcoholic steatohepatitis2009

    • Author(s)
      Anezaki, Y.
    • Journal Title

      Hepatol.Res. 39

      Pages: 609-618

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Eicosapentaenoic acid ameliorates steatohepatitis and hepatocellular carcinoma in hepatocyte-specific Pten-deficient mice2009

    • Author(s)
      Ishii, H.
    • Journal Title

      J.Hepatol. 50

      Pages: 562-571

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] The lipid phosphatase INPP4A prevents striatal neurons from excitotoxic death2010

    • Author(s)
      佐々木純子
    • Organizer
      BMB2010
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-08
    • Related Report
      2011 Final Research Report
  • [Presentation] Identification of enzymes involved in fatty acid remodeling of phosphatidylinositol2010

    • Author(s)
      井上貴雄
    • Organizer
      BMB2010
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-08
    • Related Report
      2011 Final Research Report
  • [Presentation] The lipid phosphatase INPP4A prevents striatal neurons from excitotoxic death2010

    • Author(s)
      佐々木純子
    • Organizer
      BMB2010
    • Place of Presentation
      ポートピアホテル(兵庫県)
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] リン脂質アシルトランスフェラーゼの包括的解析2009

    • Author(s)
      井上貴雄
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2009-10-24
    • Related Report
      2011 Final Research Report
  • [Book] ホスファチジルイノシトール3,4-二リン酸脱リン酸化酵素INPP4Aは、興奮毒性による神経細胞死の抑制因子である、細胞工学29(9)2010

    • Author(s)
      佐々木純子、浅沼研、佐々木雄彦
    • Publisher
      学研メディカル秀潤社
    • Related Report
      2011 Final Research Report
  • [Book] ホスホイノシタイドホスファターゼの異常と病態、実験医学28(20)2010

    • Author(s)
      佐々木純子、小藤智史、佐々木雄彦
    • Publisher
      羊土社
    • Related Report
      2011 Final Research Report
  • [Book] ホスファチシルイノシトールの脂肪酸組成を規定する酵素群の同定、実験医学28(20)2010

    • Author(s)
      井上 貴雄、新井 洋由
    • Publisher
      羊土社
    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.med.akita-u.ac.jp/~bisei/

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.med.akita-u.ac.jp/~bisei/

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.med.akita-u.ac.jp/~bisei/

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.med.akita-u.ae.jp/~bisei/

    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2018-03-28  

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