| Budget Amount *help |
¥205,920,000 (Direct Cost: ¥158,400,000、Indirect Cost: ¥47,520,000)
Fiscal Year 2013: ¥41,600,000 (Direct Cost: ¥32,000,000、Indirect Cost: ¥9,600,000)
Fiscal Year 2012: ¥41,600,000 (Direct Cost: ¥32,000,000、Indirect Cost: ¥9,600,000)
Fiscal Year 2011: ¥41,600,000 (Direct Cost: ¥32,000,000、Indirect Cost: ¥9,600,000)
Fiscal Year 2010: ¥41,600,000 (Direct Cost: ¥32,000,000、Indirect Cost: ¥9,600,000)
Fiscal Year 2009: ¥39,520,000 (Direct Cost: ¥30,400,000、Indirect Cost: ¥9,120,000)
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| Outline of Final Research Achievements |
We studied phototropin-mediated signaling components in guard cells as a model case for plant light signaling. We showed that phosphatidic acid, a phospholipid second messenger produced by ABA, inhibited protein phosphatase 1 (PP1), a positive regulator of stomatal blue light signaling. The result suggests that PP1 is a crosstalk point between blue light and ABA signaling. We identified autophosphorylation sites in phot2 by mass analysis and showed the phosphorylation of Ser-761 and Ser-763 is required for the signaling. We found a novel protein kinase named BLUS1 as a substrate for phototropins and demonstrated phosphorylation of Ser-348 in response to blue light. We demonstrated that the phosphorylation of Ser-348 is essential for the signaling and that the Ser-348 is directly phosphorylated by phototropins. We showed the expression of 11 isoforms of the H+-ATPases in guard cells and identified the isoform responsible for blue light-dependent stomatal opening as AHA1.
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